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Enigma1
Positive



Registered: 08/15/13
Posts: 977
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My fractional sterilization experiment results
#19012897 - 10/22/13 10:12 AM (10 years, 3 months ago) |
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I read about this and I do have a PC I just did it out of curiosity and for grins and giggles. It did work the first time I tried 1 8oz jar WBS. I am not pleased with one run. So I tried 3 quart jars the second time I was extra careful with the prep unlike th he first time I basically just carelessly stuck the syringe .The results where contam contam contam.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 16 hours
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Re: My fractional sterilization experiment results [Re: Enigma1]
#19012904 - 10/22/13 10:13 AM (10 years, 3 months ago) |
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--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Enigma1
Positive



Registered: 08/15/13
Posts: 977
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Re: My fractional sterilization experiment results [Re: Enigma1]
#19012917 - 10/22/13 10:17 AM (10 years, 3 months ago) |
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So if you are new do not have a PC and would like to try grains Go ahead and buy pre sterilized grains from a sponsor unless you have the spores and time to waste.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 16 hours
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Re: My fractional sterilization experiment results [Re: Enigma1]
#19012960 - 10/22/13 10:34 AM (10 years, 3 months ago) |
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Or buy just a PC....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: My fractional sterilization experiment results [Re: PussyFart]
#19012989 - 10/22/13 10:41 AM (10 years, 3 months ago) |
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Nice to see someone confirm what most of us suspected
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sytar
Radiant


Registered: 09/01/13
Posts: 381
Last seen: 9 years, 5 months
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Re: My fractional sterilization experiment results [Re: Pastywhyte] 1
#19013004 - 10/22/13 10:44 AM (10 years, 3 months ago) |
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In my microbiology labs I feel like we didn't go through half the song and dance shroomerites go through and yet I never saw anyone contam an agar plate. :?
-------------------- I post from my phone. Excuse the typos and autocorrects.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 16 hours
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Re: My fractional sterilization experiment results [Re: sytar]
#19013038 - 10/22/13 10:53 AM (10 years, 3 months ago) |
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In your labs you keep cultures for 3-4 days max most likely....you guys can get away with less than stellar sterile procedures.
Like for example using a bunsen burner and calling the air around it clean.....it may be clean enough for a 4 day old culture, but not for plates that we keep for months at a time.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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