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OfflineJahLambsbread
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Registered: 08/08/10
Posts: 684
Last seen: 6 years, 3 months
a few cloning questions
    #19011090 - 10/21/13 10:32 PM (10 years, 3 months ago)

I have petri dishes with pre-made agar.  What is isolation and when do you need to do it.  Do you put the saran wrap over the petri dish first or after you see growth? and does it need to be at a certain temperature and/or humidity to grow optimally?


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OfflinePussyFart
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Re: a few cloning questions [Re: JahLambsbread]
    #19011139 - 10/21/13 10:41 PM (10 years, 3 months ago)

http://www.mushroomvideos.com/Mushroom-Cloning

http://www.mushroomvideos.com/Strain-Isolation

http://www.mushroomvideos.com/Master-Culture-Slants

Humidity does not matter at this point, room temperature, or up to 80F is fine.

Saran wrap works to seal the two halves, or parafilm.

They should be kept wrapped at all times.


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