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NecroMyce
Mostly Ghostly



Registered: 05/12/13
Posts: 747
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Possible contam? weird black spot
#19010784 - 10/21/13 09:22 PM (10 years, 4 months ago) |
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So i inoculated jars today around 3:00pm and it is now 8pm and at one of the injection points there is some weird black spot. i have a picture. but at the same time the syringe solution had a giant spore tissue, and i shook it up so that it would break up and not get clogged in the needle. and the only thing i can think that the weird black spot would be other than a random contam would be either the spore tissue i broke up, or it could be black marks from the needle when i heated it up red hot before injection. I always wipe off the tip of my needle after flame sterilization but could of forgotten one time.
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Shr00mZ
Space Invader


Registered: 04/15/08
Posts: 1,454
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Re: Possible contam? weird black spot [Re: NecroMyce]
#19010848 - 10/21/13 09:34 PM (10 years, 4 months ago) |
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Carbon off the needle you burned with ur lighter....
Who whips the needle with alcohol anyways???
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Edited by Shr00mZ (10/21/13 09:35 PM)
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SynKyd
ctrl-alt-delite



Registered: 09/27/13
Posts: 1,554
Loc: ૐ
Last seen: 2 years, 10 months
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Re: Possible contam? weird black spot [Re: Shr00mZ]
#19010953 - 10/21/13 09:58 PM (10 years, 4 months ago) |
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you don't need to wipe after flaming the needle from what I've read-
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NecroMyce
Mostly Ghostly



Registered: 05/12/13
Posts: 747
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Re: Possible contam? weird black spot [Re: SynKyd]
#19011430 - 10/21/13 11:36 PM (10 years, 4 months ago) |
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Its more of a OCD thing cuz i figured eventually some sort of black markings might end up in the cake sub. But as long as its fine, its okay with me.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Re: Possible contam? weird black spot [Re: NecroMyce]
#19011448 - 10/21/13 11:41 PM (10 years, 4 months ago) |
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Wiping a flame sterilized tool with an unsterile substance like alcohol is like wiping shit on your face after a shower.
Chemicals sanitize, heat sterilizes....so we use heat, and heat alone to sterilize our tools.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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NecroMyce
Mostly Ghostly



Registered: 05/12/13
Posts: 747
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Re: Possible contam? weird black spot [Re: PussyFart]
#19011474 - 10/21/13 11:47 PM (10 years, 4 months ago) |
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hahah alright thanks guys
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