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InvisibleNecroMyce
Mostly Ghostly
I'm a teapot


Registered: 05/12/13
Posts: 747
Possible contam? weird black spot
    #19010784 - 10/21/13 09:22 PM (10 years, 4 months ago)

So i inoculated jars today around 3:00pm and it is now 8pm and at one of the injection points there is some weird black spot. i have a picture. but at the same time the syringe solution had a giant spore tissue, and i shook it up so that it would break up and not get clogged in the needle. and the only thing i can think that the weird black spot would be other than a random contam would be either the spore tissue i broke up, or it could be black marks from the needle when i heated it up red hot before injection. I always wipe off the tip of my needle after flame sterilization but could of forgotten one time.



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InvisibleShr00mZ
Space Invader


Registered: 04/15/08
Posts: 1,454
Re: Possible contam? weird black spot [Re: NecroMyce]
    #19010848 - 10/21/13 09:34 PM (10 years, 4 months ago)

Carbon off the needle you burned with ur lighter....

Who whips the needle with alcohol anyways???


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Edited by Shr00mZ (10/21/13 09:35 PM)


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OfflineSynKyd
ctrl-alt-delite
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Registered: 09/27/13
Posts: 1,554
Loc: ૐ
Last seen: 2 years, 10 months
Re: Possible contam? weird black spot [Re: Shr00mZ]
    #19010953 - 10/21/13 09:58 PM (10 years, 4 months ago)

:whathesaid:

you don't need to wipe after flaming the needle from what I've read-

:sun:


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InvisibleNecroMyce
Mostly Ghostly
I'm a teapot


Registered: 05/12/13
Posts: 747
Re: Possible contam? weird black spot [Re: SynKyd]
    #19011430 - 10/21/13 11:36 PM (10 years, 4 months ago)

Its more of a OCD thing cuz i figured eventually some sort of black markings might end up in the cake sub. But as long as its fine, its okay with me.


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
Trusted Cultivator
Re: Possible contam? weird black spot [Re: NecroMyce]
    #19011448 - 10/21/13 11:41 PM (10 years, 4 months ago)

Wiping a flame sterilized tool with an unsterile substance like alcohol is like wiping shit on your face after a shower.

Chemicals sanitize, heat sterilizes....so we use heat, and heat alone to sterilize our tools.


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InvisibleNecroMyce
Mostly Ghostly
I'm a teapot


Registered: 05/12/13
Posts: 747
Re: Possible contam? weird black spot [Re: PussyFart]
    #19011474 - 10/21/13 11:47 PM (10 years, 4 months ago)

hahah alright thanks guys


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