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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 5 months
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
#19007290 - 10/21/13 06:49 AM (10 years, 4 months ago) |
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Quote:
skippydude said:
Quote:
Pestilence said:
Quote:
skippydude said: If you made LC without a PC, it's not very likely to be clean 
Actually, the ingredients we use for LC are not filled with different types of endospores and such, so we don't have to pressure cook liquid cultures. A simple 30 min. boil will do. 
If ingredients are exposed to air at anytime during the process, prior to sterilization, then they have been exposed to endospores.
What about pf-tek jars? they get exposed to open air when you mix verm and brf. they still do fine without a pc..
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skippydude
Myco-curious



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Re: Is this Mycelium (or what) in LC? (pictures) [Re: spacechildo]
#19007325 - 10/21/13 07:10 AM (10 years, 4 months ago) |
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Quote:
spacechildo said:
Quote:
skippydude said:
Quote:
Pestilence said:
Quote:
skippydude said: If you made LC without a PC, it's not very likely to be clean 
Actually, the ingredients we use for LC are not filled with different types of endospores and such, so we don't have to pressure cook liquid cultures. A simple 30 min. boil will do. 
If ingredients are exposed to air at anytime during the process, prior to sterilization, then they have been exposed to endospores.
What about pf-tek jars? they get exposed to open air when you mix verm and brf. they still do fine without a pc..
PF jars are pasteurized, A process that leaves behind beneficial bacteria which battles the trich for you
Edited by skippydude (10/21/13 07:14 AM)
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Stromrider
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Registered: 06/02/13
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
#19007336 - 10/21/13 07:17 AM (10 years, 4 months ago) |
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PF jars are sterilized not pasteurized
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skippydude
Myco-curious



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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Stromrider]
#19007339 - 10/21/13 07:19 AM (10 years, 4 months ago) |
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Quote:
Stromrider said: PF jars are sterilized not pasteurized
They have to reach temps of 251F to be considered sterilized> How are you doing that on the stove top?
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Stromrider
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Stromrider]
#19007341 - 10/21/13 07:19 AM (10 years, 4 months ago) |
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Quote:
If ingredients are exposed to air at anytime during the process, prior to sterilization, then they have been exposed to endospores.
This is not really accurate
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skippydude
Myco-curious



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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
#19007345 - 10/21/13 07:21 AM (10 years, 4 months ago) |
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You can steam sterilize but, it takes about a week to kill all the endospores. They have to germinate to be killed.
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Stromrider
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
#19007347 - 10/21/13 07:22 AM (10 years, 4 months ago) |
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Quote:
skippydude said:
Quote:
Stromrider said: PF jars are sterilized not pasteurized
They have to reach temps of 251F to be considered sterilized> How are you doing that on the stove top?
You don't have to reach temps of 250 to sterilize. Killing endospores requires these high temps and grains are usually heavily laden with them and that is why we have to pc them. Things like brf and verm aren't full of endospores therefore they don't require those high temps for sterilization
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skippydude
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Stromrider]
#19007354 - 10/21/13 07:24 AM (10 years, 4 months ago) |
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Quote:
Stromrider said:
Quote:
If ingredients are exposed to air at anytime during the process, prior to sterilization, then they have been exposed to endospores.
This is not really accurate
??? Open air = possible endospores, anyday of the week! What is this test Skippy day? lol
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Stromrider
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
#19007356 - 10/21/13 07:25 AM (10 years, 4 months ago) |
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Possible yes Probable no
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skippydude
Myco-curious



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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Stromrider]
#19007360 - 10/21/13 07:28 AM (10 years, 4 months ago) |
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Quote:
Stromrider said: Possible yes Probable no
Well said
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Stromrider
This must be the place



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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
#19007367 - 10/21/13 07:32 AM (10 years, 4 months ago) |
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thanks I enjoyed our discussion
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spacechildo
proletarians rise up



Registered: 01/24/13
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude] 1
#19007594 - 10/21/13 09:18 AM (10 years, 4 months ago) |
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Quote:
skippydude said:
If ingredients are exposed to air at anytime during the process, prior to sterilization, then they have been exposed to endospores.
brf+verm is mixed in open air. they do get sterilized by steaming in a pot. if your statement was true pf-tek would need a pc too, wouldn't it?
btw,
Quote:
skippydude said: You can steam sterilize but, it takes about a week to kill all the endospores. They have to germinate to be killed.
it takes 8 hrs to steam grains in a normal pot.
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Stromrider
This must be the place



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Re: Is this Mycelium (or what) in LC? (pictures) [Re: spacechildo]
#19007652 - 10/21/13 09:43 AM (10 years, 4 months ago) |
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Quote:
spacechildo said: it takes 8 hrs to steam grains in a normal pot.
Still not a good idea. I say get a job, save some money, and buy a pc you lazy hippy!
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skippydude
Myco-curious



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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Stromrider]
#19007704 - 10/21/13 10:01 AM (10 years, 4 months ago) |
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Back to the original question Here is a LC that looks very similar to yours
 Made with LME & Dextrose which caramelized a bit in the PC It tests clean, so as previously stated, "You gotta throw some on grain to know for sure"
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Stromrider
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
#19007715 - 10/21/13 10:05 AM (10 years, 4 months ago) |
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True there is only one way to know 4 sure
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Enigma1
Positive



Registered: 08/15/13
Posts: 977
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Stromrider]
#19008689 - 10/21/13 02:00 PM (10 years, 4 months ago) |
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greenery1
Stranger

Registered: 06/11/13
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Enigma1]
#19032360 - 10/25/13 06:08 PM (10 years, 3 months ago) |
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Knocked up some BRF today, what I discovered was that the mycilium gets stuck in the syringe when trying to suck it up (18 gauge needle), I couldn't! had to take water from around it - I did do 4 new LC's last weekend and was pleased to see the two new GT jars both producing MC in 5 days. So I used those for the inoculation, and did 1 using that old GT in the original pics as a test.
So I guess one question: is the MC spread throughout the whole jar, or can you miss it if not stuck into the MC cloud?
Another issue that I realized is if you are sucking up 8-10 CC for a few jars of BRF, your supply runs out fairly quick...so I'm going to make a bigger jar of LC, also tipping the jar to reach the water is a bit disconcerting...
When mixing the VErm with water 2:1, I found quite a bit of water came out when I squeezed a handful, so I add more Verm, was still a problem, but then I was wondering if I wasn't squeezing it too hard, first time, so not sure if it was ok or not. It seemed fine after adding the BRF.
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blueconfusion
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
#19032497 - 10/25/13 06:40 PM (10 years, 3 months ago) |
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when i started making large LCs is when i ran into problems...
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skippydude
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: blueconfusion]
#19032909 - 10/25/13 08:02 PM (10 years, 3 months ago) |
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Put a marble or a piece of broken glass in your LC jug, this will help, when shaking the jar, to break up the mycelium mass when ready for use. If you don't get a little of the white stuff in each item you knock up, it is possible that nothing will grow. I've had it happen on grain and petri dishes. Go to a 14 gauge needle if you can, at least 16 gauge for LCs as 18s clog very easy.
 I use a heavy duty inoculation port, an 18 gauge needle with a .02 micron Whatman syringe filter for GE. Then draw the cultures with a separate 14g or 16g I have a magnetic stir plate so I now use that to break up the myc. Before that I was able to shake these jars with a marble in it without wetting the filter(bacteria swims right through a wet GE filter)
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greenery1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
#19043594 - 10/28/13 12:17 AM (10 years, 3 months ago) |
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Thanks Skippy. Most of my jars are growing after 2 days, a few had nothing so I re-shot them with MC, checked the syringe before to make sure I could see bits floating around it.
Is that a homemade port there in the picture? Haven't seen one like that.
I was planning to do bulk from the BRF cakes, any suggestions on a tek to follow? I was going to try Coir/Verm...
Edited by greenery1 (10/28/13 01:30 AM)
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