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InvisibleOgla
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Registered: 02/16/04
Posts: 11,320
sclerotia of galindoi forming in LC?
    #19004276 - 10/20/13 02:49 PM (10 years, 4 months ago)

has this ever happened to anyone?  got little dark spots forming which i think is sclerotia or contam.


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Invisiblestonesun
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Re: sclerotia of galindoi forming in LC? [Re: Ogla]
    #19004366 - 10/20/13 03:14 PM (10 years, 4 months ago)

Yes, it happened to me before,
although I have to clarify that the LC was already over six months old.
A solid mycelium mat started to form on the top of the liquid, followed
by sclerotia.
So not IN liquid culture, but rather ON it.


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InvisibleOgla
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Registered: 02/16/04
Posts: 11,320
Re: sclerotia of galindoi forming in LC? [Re: stonesun]
    #19004439 - 10/20/13 03:32 PM (10 years, 4 months ago)

Yeah.  Lately ive been having what looks like contam ob the surface of the liquid culture but is actually mycelium growing on top of it.  If i leave it around awhile, which often happens , it forms a thicker upper layer.  Im using karo and malt agar for my LCs.  Seems to work great


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InvisibleOgla
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Registered: 02/16/04
Posts: 11,320
Re: sclerotia of galindoi forming in LC? [Re: Ogla]
    #19004459 - 10/20/13 03:36 PM (10 years, 4 months ago)

this is wikidzon,




This is Galindoi..




looks like contam, but i dont know if it is


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InvisibleOgla
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Registered: 02/16/04
Posts: 11,320
Re: sclerotia of galindoi forming in LC? [Re: Ogla]
    #19004501 - 10/20/13 03:46 PM (10 years, 4 months ago)

here is the current instance of the wikidzon., this jar btw was the least thick of the two which i threw out the thicker one.  Has a layer of mycelium like super thick on top



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