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Offlinesaltyd
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Registered: 05/13/11
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How to not run out of spawn?
    #19001820 - 10/19/13 10:12 PM (10 years, 4 months ago)

This is my first attempt at doing a bulk grow, and I want to save some spawn so I can keep using it for new batches, kind of like a sourdough starter.  Is this possible, or does the spawn go bad?  What's the best way of ensuring that I have a constant supply of spawn on hand and I don't have to inoculate new jars all over again when I want to make a new batch?


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Invisiblehamloaf
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Re: How to not run out of spawn? [Re: saltyd]
    #19001853 - 10/19/13 10:21 PM (10 years, 4 months ago)

Master culture slants.


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Invisiblemaddchef
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Re: How to not run out of spawn? [Re: saltyd]
    #19001857 - 10/19/13 10:22 PM (10 years, 4 months ago)

Search G2G and also agar or LC


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflinePussyFart
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Re: How to not run out of spawn? [Re: maddchef]
    #19001868 - 10/19/13 10:25 PM (10 years, 4 months ago)

Quote:

maddchef said:
Search G2G and also agar



:whathesaid:


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Invisiblemaddchef
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Re: How to not run out of spawn? [Re: PussyFart]
    #19001870 - 10/19/13 10:26 PM (10 years, 4 months ago)

Lol nice edit. Guess I shouldn't say lc around a noob


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisiblehamloaf
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Loc: Oklahoma. Flag
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Re: How to not run out of spawn? [Re: maddchef]
    #19001881 - 10/19/13 10:28 PM (10 years, 4 months ago)

Nah, master culture slants are the way to have a constant and fresh source of inoculant without having to inoculate with spores over and over.  G2G & LC's are the way to expand culture.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


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Invisiblemaddchef
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Re: How to not run out of spawn? [Re: hamloaf]
    #19001891 - 10/19/13 10:31 PM (10 years, 4 months ago)

I agree with you but If he's asking how to not run out of spawn, I doubt he's going to delve into making a slant, could be wrong though but we rarely see any who jump in the deep end early and can actually swim.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisiblehamloaf
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Registered: 12/23/09
Posts: 20,455
Loc: Oklahoma. Flag
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Re: How to not run out of spawn? [Re: hamloaf]
    #19001913 - 10/19/13 10:37 PM (10 years, 4 months ago)

Take a live piece of culture out of the slant and inoculate agar mediums preferably in Petri dishes.  Cut wedges of the live cuture from the Petri dish and inoculate sterilized jars of grain.  The jars inoculated with the live culture on agar wedge will be your mother jars.  Once the mother jars are fully colonized spread them out into sterilized recieving grain jars and repeat as needed/wanted.


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InvisibleBass808
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Registered: 10/19/13
Posts: 32
Loc: Underground
Re: How to not run out of spawn? [Re: hamloaf]
    #19049763 - 10/28/13 11:47 PM (10 years, 3 months ago)

Im kinda in the same situtation as the OP, noc'd 12 WBS jars, waiting on first growth. I threw a old qt of colonized wbs outside a few weeks ago, some good storms coming thru im hoping:) Maybe ill have a pinset shortly. Im looking at trying my hand at agar.


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