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Invisiblemaynardjameskeenan
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Psilocybe silvatica * 1
    #19001057 - 10/19/13 07:16 PM (10 years, 3 months ago)

I found these in a bog, growing out of leaf litter of hardwoods and other woody debris. I think they might be silvatica, they certainly look like a Psilocybe to me. The spore prints are purple.







































Cluster around bushes are trees mostly.


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19001211 - 10/19/13 07:50 PM (10 years, 3 months ago)

This is a cool find.:thumbup:

:thisisheavyman:

:cheersyoufuck:


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Offlinethetonebone72
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Re: Psilocybe silvatica [Re: paracelsus]
    #19001251 - 10/19/13 07:57 PM (10 years, 3 months ago)

I don't have any knowledge of P. silvatica and the resources seem deficient. They sure do look psilocybe! 

Did you peel back the pellicle? Don't see any bluing on the cap margins but I do see that little bit on the stem butt.

:popcorn:


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OfflineAlan RockefellerM
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Re: Psilocybe silvatica [Re: thetonebone72]
    #19001258 - 10/19/13 07:59 PM (10 years, 3 months ago)

Are they staining blue?

Please save the collection for scientific study!


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OfflineEddeee
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Re: Psilocybe silvatica [Re: thetonebone72]
    #19001262 - 10/19/13 08:00 PM (10 years, 3 months ago)



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Don't read books study life then write books
we are nothing but atoms trying to figure out what atoms are.



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Invisiblemaynardjameskeenan
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Re: Psilocybe silvatica [Re: thetonebone72]
    #19001271 - 10/19/13 08:02 PM (10 years, 3 months ago)

Quote:

thetonebone72 said:
I don't have any knowledge of P. silvatica and the resources seem deficient. They sure do look psilocybe! 

Did you peel back the pellicle? Don't see any bluing on the cap margins but I do see that little bit on the stem butt.

:popcorn:



They do have a pellicle but it's super-thin and really hard to separate. I haven't noticed much bluing really on the caps either, a few of the cut-stems and basal-mycelium (in the right light) do kinda look blue in person.


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Invisiblemaynardjameskeenan
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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19001329 - 10/19/13 08:14 PM (10 years, 3 months ago)

Quote:

Alan Rockefeller said:
Are they staining blue?

Please save the collection for scientific study!



I was thinking of seeing if the OSU herbarium took type collections.
I really should send them out to someone who is good at micrographs, I still suck.
I will send some to the address you gave me, thank for your help.


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Edited by Alan Rockefeller (10/20/13 07:05 AM)


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Offlinecanid
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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19001348 - 10/19/13 08:18 PM (10 years, 3 months ago)

Hell, I'd be pleased to do the microscopy if I were quite competent and had the time. And I probably do have the time, lol.


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Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.


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Offlineknarkkorven
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Re: Psilocybe silvatica [Re: canid]
    #19002458 - 10/20/13 01:56 AM (10 years, 3 months ago)

Interesting find! I don't know if it's silvatica but it sure looks like a Psilocybe!


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OfflineAlan RockefellerM
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Re: Psilocybe silvatica [Re: knarkkorven]
    #19002873 - 10/20/13 07:12 AM (10 years, 3 months ago)

You should ask Joe Ammirati o study it.  And send some to Virginia Ramirez for good measure.  She is one of the best in the world with regards to Psilocybe and Deconica. 


But really the best place for this collection is the Institute of Ecology in Xalapa.

Have you considered P. liniformans?


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OfflineTetragammatron
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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19002881 - 10/20/13 07:18 AM (10 years, 3 months ago)

Handsome shrooms.

:popcorn:


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Offlinejet li
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Re: Psilocybe silvatica [Re: Tetragammatron]
    #19002936 - 10/20/13 07:51 AM (10 years, 3 months ago)

Re: P. liniformans - would be the first to my knowledge of P. liniformans in wood substrate.


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Offlinehidenseek1
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Re: Psilocybe silvatica [Re: jet li]
    #19003004 - 10/20/13 08:24 AM (10 years, 3 months ago)

can you get in trouble for sending mushrooms for study if they were active and got opened at like customs or something?


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Offlinepsylosymonreturns
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Re: Psilocybe silvatica [Re: hidenseek1]
    #19003150 - 10/20/13 09:35 AM (10 years, 3 months ago)

Quote:

hidenseek1 said:
can you get in trouble for sending mushrooms for study if they were active and got opened at like customs or something?




Fuck that , its for science. Id do it in a second.




Cool find May!! I am not sure if this fits for P silvatica. But rad none the less.


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OfflineAlan RockefellerM
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Re: Psilocybe silvatica [Re: hidenseek1]
    #19003242 - 10/20/13 10:09 AM (10 years, 3 months ago)

Quote:

hidenseek1 said:
can you get in trouble for sending mushrooms for study if they were active and got opened at like customs or something?




You could, but it is super unlikely.  Customs is used to finding big bags of cubes.  They typically do nothing for sending small amounts of drugs.  Also you do not need to put your real return address.  I asked Laura Guzman about this and she said it was totally legal for her to receive Psilocybe in the mail.  The Institutr of Ecology and most other herbariums have a permit to have psilocybin mushrooms.    Also in the usa, the drug laws only apply if you know you have drugs.  If you do not know if they are active or not, you have a good defense in court.  The reason for this is that mens rea is an element of the offense that must be proven by the government to get a conviction.

Regarding the pellicle, that mostly applies to liberty caps.  It is super easy to peel a liberty cap pellicle and significantly more difficult for other Psilocybe species.  Many non-psilocybes have a seperable pellicle, Russula for example.


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Offlinepsylosymonreturns
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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19003250 - 10/20/13 10:12 AM (10 years, 3 months ago)

The pellicle is prevelant in P pelliculosa so I would expect the same for P silvatica.

Hey nice to see you around Alan!!! :smile:


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InvisibleJoust
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Re: Psilocybe silvatica [Re: psylosymonreturns]
    #19003272 - 10/20/13 10:21 AM (10 years, 3 months ago)

hmm, there appears to be bluing, you should send one to me if you have enough.
Very cool!


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~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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OfflineAlan RockefellerM
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Re: Psilocybe silvatica [Re: psylosymonreturns]
    #19003290 - 10/20/13 10:30 AM (10 years, 3 months ago)

Quote:

psylosymonreturns said:

Fuck that , its for science. Id do it in a second.






Apparently you already have, as you were given an honorable mention in Virginia's recent Psilocybe phylogeny paper.  :smile:


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Offlinepsylosymonreturns
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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19003310 - 10/20/13 10:38 AM (10 years, 3 months ago)

Quote:

Alan Rockefeller said:
Quote:

psylosymonreturns said:

Fuck that , its for science. Id do it in a second.






Apparently you already have, as you were given an honorable mention in Virginia's recent Psilocybe phylogeny paper.  :smile:




Oh wow that is so cool!!! :smile:


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Re: Psilocybe silvatica [Re: psylosymonreturns]
    #19003450 - 10/20/13 11:23 AM (10 years, 3 months ago)

Maybe it's just me, but they look like ovoids...


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Re: Psilocybe silvatica [Re: divo]
    #19003658 - 10/20/13 12:15 PM (10 years, 3 months ago)

I have yet to find a Psilocybe or Deconica without a easily discernible pellicle.


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OfflineHashfinger
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Re: Psilocybe silvatica [Re: jet li]
    #19003679 - 10/20/13 12:20 PM (10 years, 3 months ago)

I vote liniformans.


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Species List (Georgia): Psilocybe caerulescens/weilii, Psilocybe atlantis/galindoi, Psilocybe cubensis, Psilocybe ovoideocystidiata, Psilocybe caerulipes, Psilocybe semilanceata, Psilocybe fagicola, Copelandia cyanescens, Panaeolus cinctulus, Panaeolus fimicola, Panaeolus olivaceus, Gymnopilus luteofolius, Gymnopilus aeruginosus, Gymnopilus junonius, Pluteus salicinus

(Ohio): Psilocybe ovoideocystidiata, Psilocybe caerulipes, Pluteus cyanopus, Pluteus salicinus sensu lato..., Panaeolus cinctulus, Gymnopilus luteus, Gymnopilus luteofolius, Gymnopilus junonius, Gymnopilus aeruginosus


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OfflineJoie
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Re: Psilocybe silvatica [Re: jet li]
    #19003722 - 10/20/13 12:31 PM (10 years, 3 months ago)

Quote:

jet li said:
I have yet to find a Psilocybe or Deconica without a easily discernible pellicle.



The appropriately named Deconica apelliculosa.


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OfflineJoie
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Re: Psilocybe silvatica [Re: Joie]
    #19003739 - 10/20/13 12:34 PM (10 years, 3 months ago)

A quick check later, Deconica montana also doesn't have a seperable pellicle.


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OfflineLepkaun
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Re: Psilocybe silvatica [Re: Joie]
    #19003880 - 10/20/13 01:05 PM (10 years, 3 months ago)

:popcorn:


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Offlinejet li
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Re: Psilocybe silvatica [Re: Lepkaun]
    #19003898 - 10/20/13 01:09 PM (10 years, 3 months ago)

I guess I haven't found those lol.  Also, he found these in a clearcut, woody substrate right?  So, isn't P. liniformans out?  Then again, P. stuntzii and P. baeocystis are multi-habitat species, so why not P. liniformans?


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Re: Psilocybe silvatica [Re: jet li]
    #19004220 - 10/20/13 02:33 PM (10 years, 3 months ago)

:popcorn: intresting


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Ps. Cubensis                                        Ps. Cyanescens                                      Ps. Stuntzii  *GrowLog*
   
                                                                                                      :sasquatch::mushroomgrow::leaf::mushroomgrow::leaf::mushroomgrow:
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Invisiblemaynardjameskeenan
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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19389808 - 01/08/14 06:14 PM (10 years, 22 days ago)

Quote:

Alan Rockefeller said:
You should ask Joe Ammirati o study it.  And send some to Virginia Ramirez for good measure.  She is one of the best in the world with regards to Psilocybe and Deconica. 


But really the best place for this collection is the Institute of Ecology in Xalapa.

Have you considered P. liniformans?



Hey so, I still haven't sent any of these off yet but I'd like to. I know it's been awhile but I get sidetracked easily. It hasn't been a huge priority.
Since my season is over with and I have nothing better to do I'd like to send them off to someone who can work a microscope better than me. I'm kinda worried about sending them out of the country just in case they are active.

Anyway I took some more picture of them today.








Notice how distant the stem is from the gills? All of them are like this too.
I think they may be a Deconica because they smell nothing like a Psilocybe, they smell kinda like old leaves.


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OfflineAlan RockefellerM
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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19390062 - 01/08/14 07:15 PM (10 years, 22 days ago)

Quote:

maynardjameskeenan said:
I'm kinda worried about sending them out of the country just in case they are active.







You don't have to put your real return address on it.


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Invisiblemaynardjameskeenan
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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19390071 - 01/08/14 07:18 PM (10 years, 22 days ago)

Quote:

Alan Rockefeller said:
Quote:

maynardjameskeenan said:
I'm kinda worried about sending them out of the country just in case they are active.







You don't have to put your real return address on it.



I'll send some to Mexico I suppose.
If you get me arrested my wife will gut you. :grin:


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OfflineAlan RockefellerM
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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19390085 - 01/08/14 07:21 PM (10 years, 22 days ago)

You are not going to get arrested for mailing around scientific collections.  The mail police are used to finding gallon bags of cubes, they are not going to care about a gram or so...


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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19390680 - 01/08/14 09:17 PM (10 years, 22 days ago)

This collection has been on my mind heavily lately.  I really want to know what they are!


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19391979 - 01/09/14 03:34 AM (10 years, 22 days ago)

:popcorn: :rockon:


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OfflineHarryL
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Re: Psilocybe silvatica [Re: Bubbles85]
    #19392834 - 01/09/14 09:41 AM (10 years, 22 days ago)

Interesting....

Isn't the lack of blue bruising on the stem and cap a case for them not being psilocybe? Yes, I know pells and liberty caps don't always bruise blue but this is a substantial mushroom....

Did the cap change color when dried?

First glance... I would have said ovoid myself but... There's Like 200 species of psilocybe and I have only seen a handful of those.... 

Say in a bog?  How about p. tampanensis? Or related species... Rare...

Maybe we should have a pool to guess what they are...


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Re: Psilocybe silvatica [Re: HarryL]
    #19392887 - 01/09/14 09:53 AM (10 years, 22 days ago)

I never saw this, throw one in the mail for me!:super:


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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OfflineAlonso CortesPerez
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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19393293 - 01/09/14 11:33 AM (10 years, 21 days ago)

Notice how distant the stem is from the gills? All of them are like this too.
I think they may be a Deconica because they smell nothing like a Psilocybe, they smell kinda like old leaves.





Hello, I would like to study this collection if you agree. I want to know if it is a Psilocybe or Deconica.
Psilocybe caerulipes from Mexico, very little farinaceous smell and almost no blue stain. It is possible that it is a species of Psilocybe.
Great find!


Edited by Alonso CortesPerez (01/09/14 11:41 AM)


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Invisiblemaynardjameskeenan
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Re: Psilocybe silvatica [Re: Alonso CortesPerez]
    #19394247 - 01/09/14 03:34 PM (10 years, 21 days ago)

Quote:

Alonso CortesPerez said:
Notice how distant the stem is from the gills? All of them are like this too.
I think they may be a Deconica because they smell nothing like a Psilocybe, they smell kinda like old leaves.





Hello, I would like to study this collection if you agree. I want to know if it is a Psilocybe or Deconica.
Psilocybe caerulipes from Mexico, very little farinaceous smell and almost no blue stain. It is possible that it is a species of Psilocybe.
Great find!



I'll absolutely send some to you, it's an honor.
I think Alan gave me your address.
I'll send sample out to you and Joust before the end of the weekend. :smile:
Would 5 fruit bodies be enough?


--------------------
May you be filled with loving kindness.
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May you be peaceful and at ease.
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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19394613 - 01/09/14 04:58 PM (10 years, 21 days ago)

I have a bunch from the first time I found them if you need more.  I'm excited to see the ball rolling on this one again.


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Invisiblemaynardjameskeenan
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Re: Psilocybe silvatica [Re: nomadbrad]
    #19395289 - 01/09/14 06:47 PM (10 years, 21 days ago)

I'm sure this doesn't help much, I got a few pictures of the spores and gill section (I can't make of any structures other than spores).
How to I use my stage micrometer to make measurements?
When I use the 100/1.25 OIL magnification it just looks shitty and blurry, I can't make anything out.
I'll keep trying.



Edited by maynardjameskeenan (01/09/14 06:49 PM)


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19395332 - 01/09/14 06:54 PM (10 years, 21 days ago)

You don't use a stage micrometer to make measurements, you use it to calibrate your reticle (Or software).


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Re: Psilocybe silvatica [Re: Byrain]
    #19395372 - 01/09/14 07:01 PM (10 years, 21 days ago)

Quote:

Byrain said:
You don't use a stage micrometer to make measurements, you use it to calibrate your reticle (Or software).



How is what I'm trying to get at? I don't have a reticle so I'll probably be using Piximètre.


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19395377 - 01/09/14 07:01 PM (10 years, 21 days ago)

I created this for my friends. I consider you one of them.


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Re: Psilocybe silvatica [Re: The Lightning]
    #19395433 - 01/09/14 07:11 PM (10 years, 21 days ago)

Quote:

The Lightning said:
I created this for my friends. I consider you one of them.



This is exceedingly helpful, thank you sir :hatsoff:

Will I still be able to make out structures (ie. cystidia, Basidia) without dye?


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19395440 - 01/09/14 07:12 PM (10 years, 21 days ago)

I've never used Piximètre, someone else certainly knows.

@The Lightning, does that actually contain a section on calibrating your scope for Piximètre?  I'm not seeing it.

Quote:

maynardjameskeenan said:
Will I still be able to make out structures (ie. cystidia, Basidia) without dye?




Yes, thin!!! sections are key.


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Re: Psilocybe silvatica [Re: Byrain]
    #19395494 - 01/09/14 07:23 PM (10 years, 21 days ago)

Quote:

Will I still be able to make out structures (ie. cystidia, Basidia) without dye?




HI. Normally, we use the term "stain(s)" instead of dye, unless dye is being used as the stain. The best stain you can find easily might be Congo Red. It's in the link. If you're waiting for Congo Red, KOH is an awesome rehydration liquid that allows enough visibility most of the time to make observations of cystidia. I really recommend KOH and Congo Red in separate, amber dropper bottles. Check out that *ink.

Quote:

@The Lightning, does that actually contain a section on calibrating your scope for Piximètre?  I'm not seeing it.




Not yet. Alan, if you would like to add that to the document, please let me know. If Alan doesn't add it, someone else feeling the need can provide the instructions without cost and I will add them.


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Re: Psilocybe silvatica [Re: The Lightning] * 1
    #19396113 - 01/09/14 09:45 PM (10 years, 21 days ago)

Did anyone consider Hypholoma for this?


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Re: Psilocybe silvatica [Re: inski]
    #19396293 - 01/09/14 10:32 PM (10 years, 21 days ago)

Did you have a species in mind? I can't seem to find one that resembles it yet.


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Re: Psilocybe silvatica [Re: inski]
    #19396846 - 01/10/14 12:37 AM (10 years, 21 days ago)

Quote:

inski said:
Did anyone consider Hypholoma for this?



I could see that. I dont think it is a Psilocybe species myself.


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~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: nomadbrad]
    #19397306 - 01/10/14 04:45 AM (10 years, 21 days ago)

Quote:

nomadbrad said:
Did you have a species in mind? I can't seem to find one that resembles it yet.




H. olivaceotinctum might be a suggestion, considering the habitat.


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Re: Psilocybe silvatic [Re: Anglerfish]
    #19397855 - 01/10/14 08:37 AM (10 years, 21 days ago)

Quote:

Anglerfish said:
H. olivaceotin
Quote:

nomadbrad said:
Did you have a species in mind? I can't seem to find one that resembles it yet.




H. olivaceotinctum might be a suggestion, considering the habitat.





The habitat was a bog that drains every year and it's surrounded by a diverse hardwood forest consisting of mostly maples, cotton woods, alders and oaks.  The few conifers that are there are dying or already dead.  :confused:


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Re: Psilocybe silvatic [Re: nomadbrad]
    #19397926 - 01/10/14 09:02 AM (10 years, 21 days ago)

Quote:

nomadbrad said:
The habitat was a bog




That kind of heightens the chance for one of the smaller Hypholoma species, I guess.


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Re: Psilocybe silvatic [Re: Anglerfish]
    #19399050 - 01/10/14 01:18 PM (10 years, 20 days ago)

yeah it's very possible. it's time,  I need a scope haha


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Re: Psilocybe silvatica [Re: nomadbrad]
    #19399517 - 01/10/14 02:47 PM (10 years, 20 days ago)

Quote:

nomadbrad said:
Did you have a species in mind? I can't seem to find one that resembles it yet.



No, I don't know the Hypholoma in that part of the world, it's best to determine genus first then look at the species known from that area.

Look for chrysocystidia in the hymenium and a subcellular layer below the pileipellis, if these are present then Hypholoma is likely.


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Re: Psilocybe silvatica [Re: inski]
    #19400365 - 01/10/14 05:33 PM (10 years, 20 days ago)

:notsureif:
I can't tell if this is a spore or not...


Can I temporarily substitute food coloring for stain?


Edited by maynardjameskeenan (01/10/14 05:36 PM)


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19400391 - 01/10/14 05:41 PM (10 years, 20 days ago)

The somewhat circular, brown objects are spores. Some of them appear crushed in the above pic.

When viewing brown colored spores, you should be able to get away with just using water. Try this: Take a single gill (nothing more) - and place it on a slide. Add two drops of water. Let it sit for a few seconds then grab some tweezers and remove the gill from the slide entirely. Hopefully some spores will still be on the slide. Add one more drop of water before placing a cover slip onto the area where the gill was.

Then view for spores.


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19401101 - 01/10/14 08:49 PM (10 years, 20 days ago)

Stains are entirely optional for standard microscopy, they become necessary only when trying to view obscure features like the spore nuclei in Entomophthora species or maybe to get good pictures of tiny hyaline Clitocybe spores...  How are you hydrating your slides?  Your section looks kind of thick, make it thinner, as thin as you can make it and then some.  Small sections are easier to make thin than big ones, it takes time to perfect so try not to get discouraged. :smile:


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Re: Psilocybe silvatica [Re: Byrain]
    #19401921 - 01/11/14 12:40 AM (10 years, 20 days ago)

Funny this summer I worked a 2 month contract with this lady, who during the year teaches entry biology or someshit in this college . Well after I got to know her a bit I find out she teaches microscope basics !! :wtf:
And shes stoked Im into mycology. So I'm like " YES this is my chance to learn this shit once and for all". So I brought my scope to work and she was going to give me a lesson but it turned out everything I subconsciously learned over the last year reading posts had actually sunk in and she was quite impressed.Because i have been really unsure if I was doing it right and I was. :smile:
I went for a quick walk at lunch, found a few specimens. Set up my scope,pulled out a fresh razor blade, cut the mushroom in half and sliced a thin slice of gill,dropped it on a fresh slide, dropped a bit of water on it and we were viewing spores in 2 minutes.
She was like, what the fuck , you seem to know what your doing already!! :lol: She said I was more with it then any noob she'd taught , so thanks dudes , its all because of you. I havent been in a class room since 1997 so Ive been a bit put off by it.
I need to do it more though, I still am super green. I am in the same boat as Maynard and am married with rug rats so time is hard to come by.
I need to figure out how to take pics somehow and I would like to be able to measure spores.


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Re: Psilocybe silvatica [Re: psylosymonreturns]
    #19401932 - 01/11/14 12:44 AM (10 years, 20 days ago)

Oh and how about H marginatum.


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Re: Psilocybe silvatica [Re: psylosymonreturns]
    #19401956 - 01/11/14 12:51 AM (10 years, 20 days ago)

Quote:

psylosymonreturns said:
Funny this summer I worked a 2 month contract with this lady, who during the year teaches entry biology or someshit in this college . Well after I got to know her a bit I find out she teaches microscope basics !! :wtf:
And shes stoked Im into mycology. So I'm like " YES this is my chance to learn this shit once and for all". So I brought my scope to work and she was going to give me a lesson but it turned out everything I subconsciously learned over the last year reading posts had actually sunk in and she was quite impressed.Because i have been really unsure if I was doing it right and I was. :smile:
I went for a quick walk at lunch, found a few specimens. Set up my scope,pulled out a fresh razor blade, cut the mushroom in half and sliced a thin slice of gill,dropped it on a fresh slide, dropped a bit of water on it and we were viewing spores in 2 minutes.
She was like, what the fuck , you seem to know what your doing already!! :lol: She said I was more with it then any noob she'd taught , so thanks dudes , its all because of you. I havent been in a class room since 1997 so Ive been a bit put off by it.
I need to do it more though, I still am super green. I am in the same boat as Maynard and am married with rug rats so time is hard to come by.
I need to figure out how to take pics somehow and I would like to be able to measure spores.



:congrats::heart:


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: psylosymonreturns]
    #19402372 - 01/11/14 04:44 AM (10 years, 20 days ago)

Quote:

psylosymonreturns said:
Oh and how about H marginatum.




Stems look off, they should be silky-fibrillose.
Caps should be conical to bell shaped, and with a obvious white margin.

I also never saw H. marginatum striate like that.

Although nothing is impossible, I don't think it is very likely.


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Re: Psilocybe silvatica [Re: Anglerfish]
    #19403082 - 01/11/14 10:23 AM (10 years, 20 days ago)

Stipe fibrils easily wash off and are removed with handling . It's not H capnoides or fasiculare so I just guessed the more likely Hyphloma , there's only so many in the PNW.


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Re: Psilocybe silvatica [Re: The Lightning]
    #19405001 - 01/11/14 05:58 PM (10 years, 19 days ago)

Quote:

The Lightning said:
I created this for my friends. I consider you one of them.




helluva page!!

i found it a couple months ago all on my ow...google

pleased to be able to compliment the author of it!  the effort is to be really commended.

was there ever a sticky thread of 'great links' for this forum?  if so, this should be included, if not... a good place to start one.
a subsection for id keys would be sweet too, just sayin


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Re: Psilocybe silvatica [Re: RuralAnomaly]
    #19405751 - 01/11/14 08:45 PM (10 years, 19 days ago)

Thank you for the warmth, RuralAnomaly. I'm glad you've found it of use.


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Re: Psilocybe silvatica [Re: The Lightning]
    #19418775 - 01/14/14 06:21 PM (10 years, 16 days ago)

I think I may have found some Basidium.
I know pretty much nothing about what I'm doing so please go easy on me.





I think these might be cell walls or something.



You can see the nucleus of these spores.





So I just cut a super small piece of the gill off and kept cutting it into smaller section until I could do it no more.
I mounted(?) it in water and I noticed the spores were shooting off the gill when I put it under the scope, never realized they did that.

Anyway, I sent out sample to Joust, Alonso, and one other person Alan suggested yesterday.
So for now you can look at my shitty microscopy. It's hard to see anything other than spore without stain I think.


Edited by maynardjameskeenan (01/14/14 06:27 PM)


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19418924 - 01/14/14 06:44 PM (10 years, 16 days ago)

Looks like a mixture of basidia and basidioles in the first two photos. Nice! A lot of the "nuclei" you are seeing may be oil droplets.


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Re: Psilocybe silvatica [Re: suchen]
    #19419023 - 01/14/14 07:01 PM (10 years, 16 days ago)

Quote:

suchen said:
Looks like a mixture of basidia and basidioles in the first two photos. Nice! A lot of the "nuclei" you are seeing may be oil droplets.



I didn't use any oil, I just put a small water droplet under the coverslip.


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19419034 - 01/14/14 07:03 PM (10 years, 16 days ago)

The spores have oil droplets inside them naturally. Not sure whether they are actually "oil", but that is what they are called.


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The bulb is the bulb.

The volva is the volva.

They have a very long term realtionship, but they’re “just friends.”


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Re: Psilocybe silvatica [Re: suchen]
    #19419040 - 01/14/14 07:03 PM (10 years, 16 days ago)

Quote:

suchen said:
The spores have oil droplets inside them naturally. Not sure whether they are actually "oil", but that is what they are called.



O okay, I didn't know that.
:dunce:


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19419061 - 01/14/14 07:08 PM (10 years, 16 days ago)

Those big blogs may be nuclei though like you said. I am unsure.


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Re: Psilocybe silvatica [Re: suchen]
    #19419086 - 01/14/14 07:12 PM (10 years, 16 days ago)

Is it normal for the Basidium to eject spore when you put water on the gills?
It was really cool looking, I wish I had gotten a video of it, it looked kinda like mini-fireworks.


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19420043 - 01/14/14 10:23 PM (10 years, 16 days ago)

Quote:

maynardjameskeenan said:
Is it normal for the Basidium to eject spore when you put water on the gills?






No, I have never see basidia eject spores.

The spores look about the right shape for P. silvatia, P. pelliculosa has longer spores and P. semiinconspicua has wider spores.


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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19420068 - 01/14/14 10:28 PM (10 years, 16 days ago)

Quote:

Alan Rockefeller said:
Quote:

maynardjameskeenan said:
Is it normal for the Basidium to eject spore when you put water on the gills?






No, I have never see basidia eject spores.

The spores look about the right shape for P. silvatia, P. pelliculosa has longer spores and P. semiinconspicua has wider spores.





:popcorn:  I can barely stand the anticipation :rotfl:


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Re: Psilocybe silvatica [Re: nomadbrad]
    #19420093 - 01/14/14 10:34 PM (10 years, 16 days ago)

Quote:

nomadbrad said:
Quote:

Alan Rockefeller said:
Quote:

maynardjameskeenan said:
Is it normal for the Basidium to eject spore when you put water on the gills?






No, I have never see basidia eject spores.

The spores look about the right shape for P. silvatia, P. pelliculosa has longer spores and P. semiinconspicua has wider spores.





:popcorn:  I can barely stand the anticipation :rotfl:



:justcantwait:


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19423781 - 01/15/14 04:58 PM (10 years, 15 days ago)

I've noticed that if I crush the sample under the coverslip I can see things much better.
cystidia maybe :shrug:






The basidium look like they all have two sterigma



I'll try to get the micrometer and piximetre setup so I can start measuring stuff.


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19423875 - 01/15/14 05:15 PM (10 years, 15 days ago)

:popcorn:


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19424037 - 01/15/14 05:45 PM (10 years, 15 days ago)

Quote:

maynardjameskeenan said:
I've noticed that if I crush the sample under the coverslip I can see things much better.





:emotionalmoment:

They grow up so fast.


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The bulb is the bulb.

The volva is the volva.

They have a very long term realtionship, but they’re “just friends.”


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19424139 - 01/15/14 06:12 PM (10 years, 15 days ago)

Quote:

maynardjameskeenan said:
I've noticed that if I crush the sample under the coverslip I can see things much better.
cystidia maybe :shrug:






The cystidia maybe are basidia and basidioles. 
I see both two and 4 spored basidia in your last photo.


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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19439027 - 01/18/14 05:59 PM (10 years, 12 days ago)

These were the photos I took of this species when I first found them.  I knew they were strange and interesting, luckily I showed MJK and he assured me that we needed to document them and have them looked at.

They look like a different species in every pic.



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Re: Psilocybe silvatica [Re: nomadbrad]
    #19440268 - 01/18/14 11:14 PM (10 years, 12 days ago)

beautiful!


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19440288 - 01/18/14 11:19 PM (10 years, 12 days ago)

Super cool!! Thanks for sharing more pics! I cant wait to see some results .


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OfflineDr.Tooty
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Re: Psilocybe silvatica [Re: psylosymonreturns]
    #19440406 - 01/18/14 11:49 PM (10 years, 12 days ago)

Sweet, lets you see some good variances.


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Re: Psilocybe silvatica [Re: Dr.Tooty]
    #19442082 - 01/19/14 11:22 AM (10 years, 12 days ago)

:popcorn::popcorn::popcorn:


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Re: Psilocybe silvatica [Re: Lepkaun]
    #19443232 - 01/19/14 03:32 PM (10 years, 11 days ago)

i will be :popcorn::takingnotes::crazy2: tonight... got em


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19444516 - 01/19/14 08:44 PM (10 years, 11 days ago)

:wooyeah:


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Re: Psilocybe silvatica [Re: rhizoRider]
    #19445781 - 01/20/14 02:00 AM (10 years, 11 days ago)

Good day/night everyone! I haven't posted on here in a long time, but this thread rocks, props to the op! :rockon:


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Re: Psilocybe silvatica [Re: kyguy]
    #19446456 - 01/20/14 07:53 AM (10 years, 11 days ago)

:popcorn:


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Re: Psilocybe silvatica [Re: Joust]
    #19447927 - 01/20/14 02:18 PM (10 years, 10 days ago)

Quote:

Joust said:
i will be :popcorn::takingnotes::crazy2: tonight... got em



:idhitit:


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Re: Psilocybe silvatica [Re: Lepkaun]
    #19450014 - 01/20/14 09:42 PM (10 years, 10 days ago)

Quote:

Lepkaun said:
Quote:

Joust said:
i will be :popcorn::takingnotes::crazy2: tonight... got em



:idhitit:



im working on it...not at all. actualy just got done writing 8 pages  for class. going to watch archer. then probably after !


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19450295 - 01/20/14 10:30 PM (10 years, 10 days ago)

Joust! :psycrankey:  too much :whatever:

I still think your rad  :popcorn:

Seriously though..... :grin:


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Re: Psilocybe silvatica [Re: nomadbrad]
    #19450382 - 01/20/14 10:45 PM (10 years, 10 days ago)

:justcantwait:  :standingby:


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Re: Psilocybe silvatica [Re: nomadbrad]
    #19450726 - 01/21/14 12:04 AM (10 years, 10 days ago)

Quote:

nomadbrad said:
These were the photos I took of this species when I first found them.  I knew they were strange and interesting, luckily I showed MJK and he assured me that we needed to document them and have them looked at.





Oh ok, have you mailed them to Mexico yet?  That is really where they need to go, I can have the world experts examine them and save the collection for ever. 

Also you can mail it to me and I will check it out, but I am kind of a dumbass compared to the Mexicans....

If you mail it to both places we can work on it together and get you something resembling an answer.



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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19450758 - 01/21/14 12:08 AM (10 years, 10 days ago)

Quote:

maynardjameskeenan said:

Anyway, I sent out sample to Joust, Alonso, and one other person Alan suggested yesterday.





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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19450887 - 01/21/14 12:37 AM (10 years, 10 days ago)

Ok I guess I will just have to wait then.  It usually takes about a month for the mail to arrive in Mexico, so it might be there around now.

Did you label it in a way that they will know what it is when they see it, or will I have to be like...hey man, did any mushrooms come in the mail recently?  Must be a maynardjameskeenan collection...

All of the collections should have the mushroomobserver observation number written on the bag, that way they will know what it is and customs won't.  I told them to expect mushrooms in the mail with numbers written on them, so they will know exactly what to do if they get a package like that...


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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19451138 - 01/21/14 01:52 AM (10 years, 10 days ago)

Quote:

Alan Rockefeller said:
Ok I guess I will just have to wait then.  It usually takes about a month for the mail to arrive in Mexico, so it might be there around now.

Did you label it in a way that they will know what it is when they see it, or will I have to be like...hey man, did any mushrooms come in the mail recently?  Must be a maynardjameskeenan collection...

All of the collections should have the mushroomobserver observation number written on the bag, that way they will know what it is and customs won't.  I told them to expect mushrooms in the mail with numbers written on them, so they will know exactly what to do if they get a package like that...






With the package I included a note; on it was the mushroomobserver.org observation number, the date of the collection, and what state and county they were found in.
I put them in a zip-lock bag and put them in a DVD case so they won't get damaged in en route, they are also in a bubble-wrap envelope.

I sent out three packages, One to whoever Womperknicker is, Joust and one to el Instituto de Ecologia.


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19451186 - 01/21/14 02:15 AM (10 years, 10 days ago)

I have received mine, but felt guilty for not finishing my last project, i may have done enough work to move ID it now. Ill get to this soon so at the very least we can see what we are looking at as far as sizes and basic micro features. I wish i were a special mexican mycologist...


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19451264 - 01/21/14 02:57 AM (10 years, 10 days ago)

You'll always be my special Mexican mycologist  :hugitout:


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Re: Psilocybe silvatica [Re: Joust]
    #19451280 - 01/21/14 03:09 AM (10 years, 10 days ago)

Quote:

Joust said:
I wish i were a special mexican mycologist...




Yes I don't know about that, the problem is that they do not actually get paid.  So yea, its a cool job studying Psilocybes, but you show up every day and the paycheck never comes, so how cool is it really?    Well the cool thing is that they do it for the love of mushrooms, so whatever you send will be fully appreciated.

So maybe you wish you were there and maybe you don't, but it's not a job that I would take.


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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19451291 - 01/21/14 03:17 AM (10 years, 10 days ago)

Quote:

Alan Rockefeller said:
So maybe you wish you were there and maybe you don't, but it's not a job that I would take.




Ok I am going to revise this post.  It is an awesome job, and I would take it, in fact I did for three weeks last month.  But then I left.

Its a lot of work.  There are a lot of type collections.  Some of them only have a few micrograms of material.

So yes, it is the most important work in the world, paid or not.  Is it my job?  I don't know, I hope Alonso does it.  That will save me 30 years of work.


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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19452551 - 01/21/14 12:06 PM (10 years, 9 days ago)

:seriousthankyou: for your dedication Alan!
And :bow2: :hatsoff: special Mexican mycologist.


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Re: Psilocybe silvatica [Re: Lepkaun]
    #19464937 - 01/23/14 09:26 PM (10 years, 7 days ago)



8.07 5.19
7.97 5.00
7.85 4.98
7.77 5.19
7.36 5.21
7.82 5.03
7.70 5.46
8.00 4.96
7.77 5.27
7.74 5.12

7.4 [7.7 ; 7.9] 8.2 x 4.8 [5 ; 5.2] 5.4 µm
Q = 1.4 [1.5 ; 1.6] 1.7 ; N = 10 ; C = 95%
Me = 7.8 x 5.1 µm ; Qe = 1.5



8.16 4.78
8.12 4.80
7.92 4.98
7.52 5.12
7.82 5.08
8.16 5.44
8.05 4.78
7.97 4.82
8.25 5.03
7.83 4.53
8.34 5.00
8.71 5.19
8.40 4.86
7.50 4.37
8.12 5.31
8.08 4.82

7.5 [7.9 ; 8.2] 8.7 x 4.4 [4.8 ; 5.1] 5.5 µm
Q = 1.5 [1.6 ; 1.7] 1.8 ; N = 16 ; C = 95%
Me = 8.1 x 4.9 µm ; Qe = 1.6

"The spores are dark purplish brown, (6.6)8.5 — 9.5(11) x (3.8)4 — 5.5(6) µm from 4-spored basidia, which are sometimes 2-spored."
http://en.wikipedia.org/wiki/Psilocybe_silvatica


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Edited by maynardjameskeenan (01/23/14 09:50 PM)


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19465358 - 01/23/14 11:00 PM (10 years, 7 days ago)

much better, ill break out mine tonight... doing better in school, been taking a sabatical fom mushies lol


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19465918 - 01/24/14 02:13 AM (10 years, 7 days ago)

Those spores look collapsed, perhaps they were not rehydrated properly?  I wonder if they would have the same measurements if rehydrated in KOH or isopropanol?


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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19465977 - 01/24/14 03:00 AM (10 years, 7 days ago)

I can do alcohol tomorrow.


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19467309 - 01/24/14 12:07 PM (10 years, 6 days ago)

yeah, ill measure my collections after my big test today, i ended up witing a paper instead of working on the collection. Ive just been so swamped. I havent even touched my new camera and lens


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19467831 - 01/24/14 01:59 PM (10 years, 6 days ago)

Your priorities are in the right place, school/work/family always comes first.


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19468665 - 01/24/14 05:30 PM (10 years, 6 days ago)

mushrooms = school
mushrooms = friends
mushrooms = family
i want mushrooms to = work


im free for the weekend!


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19481382 - 01/27/14 01:31 PM (10 years, 3 days ago)

:whathesaid::lahey:


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Re: Psilocybe silvatica [Re: Lepkaun]
    #19481679 - 01/27/14 02:44 PM (10 years, 3 days ago)

shit guys im working on it, my hand got a bad burn this weekend


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19482163 - 01/27/14 04:16 PM (10 years, 3 days ago)

:burn:
Lol jk, thanks for all your help my friend!
Just do what you comfortably can
I'm in no hurry for anything.


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19482470 - 01/27/14 05:30 PM (10 years, 3 days ago)

i am, i wanna know, "I will do it tonight'!:lol::shrug:

But actaullyi probably will, im really pissed off so i probably will.


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19482473 - 01/27/14 05:30 PM (10 years, 3 days ago)

I get the joke now :super::lmafo::facepalm3:


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19489123 - 01/28/14 10:53 PM (10 years, 2 days ago)

OKOKOKOKOKOKOKOKOKOKOKOKOKKKOKOKKOKOKKKOKOKOKKOk...... im doing it... now.. right this second im getting off my bed, well to the side of my bed... and scoping.... im excited


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19489170 - 01/28/14 11:04 PM (10 years, 2 days ago)

:standingby:


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19489227 - 01/28/14 11:16 PM (10 years, 2 days ago)

Quote:

maynardjameskeenan said:
:standingby:



:lol:


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19489340 - 01/28/14 11:38 PM (10 years, 2 days ago)



8.71 5.00
8.66 5.03
7.92 5.01
8.38 5.31
8.39 5.42
8.49 5.75
7.68 4.71
8.29 5.01
8.52 4.64
8.84 5.16
8.60 5.08
8.34 5.03
7.93 4.71
8.11 4.98
9.62 5.48
8.71 4.82
8.03 4.61
8.52 5.13
8.45 5.82
8.69 5.65
9.12 4.95
8.18 5.39
6.27 5.53

7.2 [8.1 ; 8.6] 9.6 x 4.5 [5 ; 5.3] 5.8 µm
Q = 1.3 [1.6 ; 1.7] 1.9 ; N = 23 ; C = 95%
Me = 8.4 x 5.1 µm ; Qe = 1.6


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OfflineAnglerfishM
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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19489940 - 01/29/14 05:29 AM (10 years, 2 days ago)

What do you think?

I'm tempted to say the spore shape looks off for Psilocybe silvatica.

It does, however, along with the measurements, look quite similar
to that of Hypholoma dispersum:

http://www.flickr.com/photos/19369983@N06/8451326755/

Mind you, this is my vague and inexperienced opinion.

Did you find cystidia?


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Re: Psilocybe silvatica [Re: Anglerfish]
    #19490232 - 01/29/14 08:21 AM (10 years, 2 days ago)

Quote:

Anglerfish said:
What do you think?

I'm tempted to say the spore shape looks off for Psilocybe silvatica.

It does, however, along with the measurements, look quite similar
to that of Hypholoma dispersum:

http://www.flickr.com/photos/19369983@N06/8451326755/

Mind you, this is my vague and inexperienced opinion.

Did you find cystidia?




This unknown was found in hardwoods not conifer.  There was no flushing of green on any of the specimens which we had at all stages of development. Also our collections of this mushroom all had a distinct spore covered annular zone, just a few inconsistencies with name this as Hypholoma dispersum.  Then again I am way more inexperienced then you are and the few descriptions of Hypholoma dispersum I could find were not complete.


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Re: Psilocybe silvatica [Re: nomadbrad]
    #19491329 - 01/29/14 12:59 PM (10 years, 1 day ago)

I've seen Hypholoma dispersum a few times in clear-cuts, I don't think theses are them, I'm no expert on the matter.
I'm just guessing these are a Psilocybe or Deconica because that's what the look most like to me, macroscopically speaking :shrug:
What should the spore shape of silvatica look like?
We'll know soon enough, Joust has a sample, Alonzo also has some, and they are much more experienced at what to look for then myself.
Until we have some solid micrographs, everything is conjecture.


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19491432 - 01/29/14 01:27 PM (10 years, 1 day ago)

Quote:

maynardjameskeenan said:
I'm just guessing these are a Psilocybe or Deconica because that's what the look most like to me, macroscopically speaking :shrug:




Well they surely look like good contenders. Although it would be good to eliminate any
possible competition, like similar looking Hypholoma sp.

Quote:


What should the spore shape of silvatica look like?




(1) Not being too easy with the terminology of shapes, and (2) having very little to go by
conserning P. silvatica, I'd say they are not ellipsoid in the way these are - they are narrower
and more pointed in the ends. But again, without the necessary experience and so little
to compare by I wouldn't try to make any secure statements.

Quote:


We'll know soon enough, Joust has a sample, Alonzo also has some, and they are much more experienced at what to look for then myself.
Until we have some solid micrographs, everything is conjecture.




True. Can't wait for more information.


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Re: Psilocybe silvatica [Re: Anglerfish]
    #19492250 - 01/29/14 04:33 PM (10 years, 1 day ago)

:popcorn:


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Re: Psilocybe silvatica [Re: Lepkaun]
    #19492467 - 01/29/14 05:07 PM (10 years, 1 day ago)

BTW - where is Workman...?


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Re: Psilocybe silvatica [Re: Anglerfish]
    #19492622 - 01/29/14 05:33 PM (10 years, 1 day ago)

spores are odd, havent done much, but they are brown and small.:lol:
im taking a time lapse so im waiting to measure... laptop is remote


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

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Re: Psilocybe silvatica [Re: Joust]
    #19492664 - 01/29/14 05:38 PM (10 years, 1 day ago)

Quote:

Joust said:
spores are odd, havent done much, but they are brown and small.:lol:
im taking a time lapse so im waiting to measure... laptop is remote




Efffff.....BROWN!?!  God damnit joust don't put the cart before the horse :laugh2:  I'm totally kidding.  No rush Mr. time lapse, you can't rush something like that.


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Re: Psilocybe silvatica [Re: nomadbrad]
    #19494144 - 01/29/14 10:32 PM (10 years, 1 day ago)

If the spore deposit is brown and not at all purple-brown then also consider Phaeogalera.


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Re: Psilocybe silvatica [Re: inski]
    #19494177 - 01/29/14 10:44 PM (10 years, 1 day ago)

Quote:

inski said:
If the spore deposit is brown and not at all purple-brown then also consider Phaeogalera.



wow im almost certain thats what it is! wow... Ill post up micro soon, though i havent compared with anything yet


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19494361 - 01/29/14 11:41 PM (10 years, 1 day ago)

Ok guys the spores are more pale than i thought, I think we can rule out psilocybe and deconica. I think. Im still working on it as we speak. I wanted to thank MJK FOR SENDING PLENTY OF MATERIAL. Makes it so much easier, great sections! im stoked.4 spored basidia, some are really long some not so much. Its hard to seperate basidioled from any cystidia at this point. looking for some cheilo now


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

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Re: Psilocybe silvatica [Re: Joust]
    #19494403 - 01/29/14 11:51 PM (10 years, 1 day ago)

cheilocystidia are narrowly utriform. Making any pleuro look like basidioles.

I also found a nice colorful golden cystida, not sure if its actually chrysocystidia. but im pretty certain it is.


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

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Re: Psilocybe silvatica [Re: Joust]
    #19494489 - 01/30/14 12:14 AM (10 years, 1 day ago)

caulo cheilo pleuro chryso are all there,


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

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Re: Psilocybe silvatica [Re: Joust]
    #19494691 - 01/30/14 01:41 AM (10 years, 1 day ago)

Look at the pileipellis, if the surface consists of cylindrical hyphae that are lying flat and just below that layer there are inflated hyphae, chrysocystidia are present and the spores are brownish in deposit, smooth walled with an apical germ pore then it is likely a Hypholoma species.

Are you using K0H to mount your sections?


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Re: Psilocybe silvatica [Re: inski]
    #19496895 - 01/30/14 02:51 PM (10 years, 20 hours ago)

:crankey: I'd like to see some micrographs instead of just unfounded statements. 
I realize you maybe busy, so take your time if necessary.


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19497092 - 01/30/14 03:21 PM (10 years, 20 hours ago)

You can't imagine how long it takes to post useful micrographs, patience is necessary!


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Re: Psilocybe silvatica [Re: Byrain]
    #19497131 - 01/30/14 03:29 PM (10 years, 19 hours ago)

:goodluck::popcorn:


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Re: Psilocybe silvatica [Re: Lepkaun]
    #19497390 - 01/30/14 04:18 PM (10 years, 19 hours ago)

nah more like TIMe lapse is using my measuring comp. i will upload today its all done.


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19497391 - 01/30/14 04:19 PM (10 years, 19 hours ago)

Quote:

maynardjameskeenan said:
:crankey: I'd like to see some micrographs instead of just unfounded statements. 
I realize you maybe busy, so take your time if necessary.



it was more notes for my sake


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19497497 - 01/30/14 04:41 PM (10 years, 18 hours ago)

Quote:

maynardjameskeenan said:
:crankey: I'd like to see some micrographs instead of just unfounded statements. 
I realize you maybe busy, so take your time if necessary.




holy fuck bud! :lol:


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Re: Psilocybe silvatica [Re: psylosymonreturns]
    #19498058 - 01/30/14 06:56 PM (10 years, 16 hours ago)

Quote:

psylosymonreturns said:
Quote:

maynardjameskeenan said:
:crankey: I'd like to see some micrographs instead of just unfounded statements. 
I realize you maybe busy, so take your time if necessary.




holy fuck bud! :lol:



I apologize if that came off as krass, I just didn't want it to be a "that's all she wrote" without any hard evidence.

Joust if you're finding these things, I don't understand why you're not just taking pictures of it and posting them, the hard part is finding them right?
I'm relatively new to the microscopy thing, so maybe I'm wrong IDK... :shrug:


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19498083 - 01/30/14 07:03 PM (10 years, 16 hours ago)

Quote:

maynardjameskeenan said:
Quote:

psylosymonreturns said:
Quote:

maynardjameskeenan said:
:crankey: I'd like to see some micrographs instead of just unfounded statements. 
I realize you maybe busy, so take your time if necessary.




holy fuck bud! :lol:



I apologize if that came off as krass, I just didn't want it to be a "that's all she wrote" without any hard evidence.

Joust if you're finding these things, I don't understand why you're not just taking pictures of it and posting them, the hard part is finding them right?
I'm relatively new to the microscopy thing, so maybe I'm wrong IDK... :shrug:



I am taking pictures, i have to use both a mac and a pc to measure them, edit them, and i take the pics with a adapter on my t3i, and upload them, while trying to find an ID.

Hypholoma olivaceotinctum slightly smaller spore spore length... though from talking to others, it seems like the original description might have been off. I dont have the monograph though... i need it.


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19498188 - 01/30/14 07:27 PM (10 years, 15 hours ago)



--------------------
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_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19498193 - 01/30/14 07:27 PM (10 years, 15 hours ago)

I think this was the coolest one


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

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Re: Psilocybe silvatica [Re: Joust]
    #19498388 - 01/30/14 08:05 PM (10 years, 15 hours ago)

Nice work joust! That micrograph you posted here is super awesome!

Spore color purplish to black with a bright reddish brown stem. :confused:


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Re: Psilocybe silvatica [Re: nomadbrad]
    #19498429 - 01/30/14 08:15 PM (10 years, 15 hours ago)




This is the best example of the spore print color, the picture I took on foil are too dark to see very well.
I still have that piece of that foil laying around, the spore in person look brown-purple to me. :shrug:


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Re: Psilocybe silvatica [Re: nomadbrad]
    #19498863 - 01/30/14 09:32 PM (10 years, 13 hours ago)

Quote:

nomadbrad said:
Nice work joust! That micrograph you posted here is super awesome!

Spore color purplish to black with a bright reddish brown stem. :confused:



ahh shit, maybe not... I dont have the KEY! AHHH


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19498994 - 01/30/14 10:03 PM (10 years, 13 hours ago)

Quote:

Joust said:
Quote:

nomadbrad said:
Nice work joust! That micrograph you posted here is super awesome!

Spore color purplish to black with a bright reddish brown stem. :confused:



ahh shit, maybe not... I dont have the KEY! AHHH




It's all good, I'm just playing devils advocate here for the sake of an accurate ID.  How do you go about getting the key?  Is it possible your keying out the wrong genus?  I'm sorry if that sounds stupid but I'm not 100% sure microscopically what makes that a for sure Hypholoma. Maybe enlighten me kind sir? 


If your sick of this I understand dude! Sorry. :heart:


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Re: Psilocybe silvatica [Re: nomadbrad]
    #19499024 - 01/30/14 10:11 PM (10 years, 13 hours ago)



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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19499332 - 01/30/14 11:45 PM (10 years, 11 hours ago)

im not certain at all. I really know nothing of micro features besides the genus psilocybe. i have only been scoping for like a year... so i need more exp to say, thats why i try to post them up and then have byrain tell me the genus.... the physical work is done at least


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19499496 - 01/31/14 01:13 AM (10 years, 10 hours ago)

Has anyone though of maybe you "scopers" put a list of specific species that are of particular interest in finding, and give details on their habitat, fruiting temps, etc.  Perhaps you may find some adventurous people who would keep an eye out, or actively look for these specific types.


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Re: Psilocybe silvatica [Re: Dren]
    #19499500 - 01/31/14 01:18 AM (10 years, 10 hours ago)

im reading this now if anyone is interested
http://www.scielo.br/scielo.php?pid=S0102-33062007000300008&script=sci_arttext
and now this, trying to find features of hypholoma
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449000/
cool stuff, wish i had more time.. :sad:


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


Edited by Joust (01/31/14 01:26 AM)


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OfflineDren
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Re: Psilocybe silvatica [Re: Joust]
    #19499508 - 01/31/14 01:28 AM (10 years, 9 hours ago)

I don't think I can quite make it down to brazil on the weekends. :grin:


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Re: Psilocybe silvatica [Re: Dren]
    #19502745 - 01/31/14 06:46 PM (9 years, 11 months ago)

I am going to look for chrysocystidia, which separates Hypholoma from Psilocybe


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19502758 - 01/31/14 06:51 PM (9 years, 11 months ago)

Quote:

Joust said:
I am going to look for chrysocystidia, which separates Hypholoma from Psilocybe




I was hoping you would, I wasn't convinced of the one you might of found.  You da man joust, thanks for dedicating time to our collection.  I really hope to meet you someday.

Dude, I've learned soooo much from this thread because I haven't got the answer yet and I've been reading and reading and defining terms like crazy.  I'm hoping this lasts a little longer! :rotfl:


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Re: Psilocybe silvatica [Re: nomadbrad]
    #19502767 - 01/31/14 06:53 PM (9 years, 11 months ago)

Quote:

nomadbrad said:
Quote:

Joust said:
I am going to look for chrysocystidia, which separates Hypholoma from Psilocybe




I was hoping you would, I wasn't convinced of the one you might of found.  You da man joust, thanks for dedicating time to our collection.  I really hope to meet you someday.

Dude, I've learned soooo much from this thread because I haven't got the answer yet and I've been reading and reading and defining terms like crazy.  I'm hoping this lasts a little longer! :rotfl:



yeah i know right! let me know if you find a good key.


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: nomadbrad]
    #19502780 - 01/31/14 06:56 PM (9 years, 11 months ago)

We should still gets some results from Alonzo so this bus isn't pulling over anytime soon :wink:


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19507560 - 02/01/14 06:45 PM (9 years, 11 months ago)

K i spend three more hours chugging along on it today.


Managed to find out theres either no pleurocystidia, or that the pluerocystidia is very small rembling basidioles, but theres quite a few of them. I didnt find any chrysocystidia, so I think that rules out Hypholoma, I would check any mycelium at the base for Stropharia Acanthocytes, I dont its that genus though...
Freaking A is this just Psilocybe silvatica, i was just reviewing and its pretty freaking close....


Heres all the micrographs.


































--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


Edited by Joust (02/01/14 07:08 PM)


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Re: Psilocybe silvatica [Re: Joust]
    #19507899 - 02/01/14 07:59 PM (9 years, 11 months ago)

Quote:

Joust said:

Freaking A is this just Psilocybe silvatica, i was just reviewing and its pretty freaking close....





:solidnod:  :fuckinawesome:  :likeaboss:
I'm excited to hear you say this, welcome to the dark-side
The habitat and description seems very similar to P. silvatica IMO
Exciting stuff!


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Re: Psilocybe silvatica [Re: Joust]
    #19507913 - 02/01/14 08:03 PM (9 years, 11 months ago)

If these really all Psilocybe silvatica, then all the pictures up on your guide for Psilocybe silvatica are probably Psilocybe pelliculosa.


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Re: Psilocybe silvatica [Re: kcrocker802]
    #19507921 - 02/01/14 08:05 PM (9 years, 11 months ago)

Quote:

kcrocker802 said:
If these really all Psilocybe silvatica, then all the pictures up on your guide for Psilocybe silvatica are probably Psilocybe pelliculosa.



I'm almost sure that the "Psilocybe silvatica" pictures in PMOTW are of P. pelliculosa.


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May you be filled with loving kindness.
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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19508036 - 02/01/14 08:35 PM (9 years, 11 months ago)

Quote:

maynardjameskeenan said:
Quote:

kcrocker802 said:
If these really all Psilocybe silvatica, then all the pictures up on your guide for Psilocybe silvatica are probably Psilocybe pelliculosa.



I'm almost sure that the "Psilocybe silvatica" pictures in PMOTW are of P. pelliculosa.



yes... i agree!


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19508046 - 02/01/14 08:37 PM (9 years, 11 months ago)

Its just weird, i wish i knew more about general micro features of genera, Its either Psilocybe silvatica or not even in the genus psilocybe haha


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19508516 - 02/01/14 10:47 PM (9 years, 11 months ago)

Well that's not very conclusive then! :lol: Alan! Where are you?


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Re: Psilocybe silvatica [Re: Joust]
    #19508541 - 02/01/14 10:52 PM (9 years, 11 months ago)

You should try to get some 1000x cystidia pics in a crush mount.  Start with nothing but tiny!!!!! piece of the gill face and then smash it! You can then repeat that with the gill edge.


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Re: Psilocybe silvatica [Re: Byrain]
    #19508570 - 02/01/14 10:58 PM (9 years, 11 months ago)

Quote:

Byrain said:
You should try to get some 1000x cystidia pics in a crush mount.  Start with nothing but tiny!!!!! piece of the gill face and then smash it! You can then repeat that with the gill edge.



my sections were well enough to crush with my fine metal tip. I observed tons of cheilo, but no real pleuro, besides those little guys, i even did what i used to do (the method you stated above) to make sure i was isolating everything and i just got these guys as pleuro



see the cell there in the middle


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Byrain]
    #19508580 - 02/01/14 11:00 PM (9 years, 11 months ago)

Quote:

Byrain said:
You should try to get some 1000x cystidia pics in a crush mount.  Start with nothing but tiny!!!!! piece of the gill face and then smash it! You can then repeat that with the gill edge.



i can either get 800x or 2000x, the ones above are 800x i can look at 1000x but i cant take micrographs cuz my adapter is at 20x not 10


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19508601 - 02/01/14 11:03 PM (9 years, 11 months ago)

Get out your point & shoot and aim it down the eyepiece?


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Re: Psilocybe silvatica [Re: Byrain]
    #19508615 - 02/01/14 11:05 PM (9 years, 11 months ago)

Quote:

Byrain said:
Get out your point & shoot and aim it down the eyepiece?



cant do measurements that way though. im not sure what you are wanting, cystidia measurements or are you wanting to see cheilo and pleruo morphology, cheilo is easy to capture, but i spend 2 hours looking for pleuro and only found those little guys.

Thanks for the input Byrain, just trying to work something out here. Soon ill be able to use the nice university scopes!~!


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19508638 - 02/01/14 11:10 PM (9 years, 11 months ago)

Ill do my best to get some micrographs out of cytidia


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19508677 - 02/01/14 11:19 PM (9 years, 11 months ago)

Both would be nice, but I was thinking morphology.  Look for more of these cells, are they not pleurocystidia?



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Re: Psilocybe silvatica [Re: Byrain]
    #19508692 - 02/01/14 11:23 PM (9 years, 11 months ago)

Quote:

Byrain said:
Both would be nice, but I was thinking morphology.  Look for more of these cells, are they not pleurocystidia?





those are all on the gill edge, those are all Cheilocystidia, thats the sterile band.


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19508699 - 02/01/14 11:24 PM (9 years, 11 months ago)

Ah shit, I thought that was labeled pleurocystidia, I should go to bed...

Yea, your pleurocystidia images look like basidioles.


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Re: Psilocybe silvatica [Re: Byrain]
    #19508718 - 02/01/14 11:28 PM (9 years, 11 months ago)

Quote:

Byrain said:
Ah shit, I thought that was labeled pleurocystidia, I should go to bed...

Yea, your pleurocystidia images look like basidioles.



thats what i thought tooo. check this out!

heres the 2000x CHEILOcystidia :lol::heart:







--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19508722 - 02/01/14 11:30 PM (9 years, 11 months ago)

Nice. :thumbup:


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Re: Psilocybe silvatica [Re: Byrain]
    #19508739 - 02/01/14 11:38 PM (9 years, 11 months ago)

Quote:

Byrain said:
Nice. :thumbup:



some of them look like thumb ups haha


They sure do look like psilocybe cheilo to my eye. Alonso is going to be helpful on this one, i asked him about it.


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust] * 1
    #19508784 - 02/01/14 11:57 PM (9 years, 11 months ago)

:trippinbawelz::hotidea:

This is awesome news. Although I just toured 5 micro breweries this evening and tried 40+ beers I'm still stoked and coherent enough to understand the ramifications of not finding chrysostidia!

Joust thanks for not just dicking this specimen off as Hypholoma! Haha love you dude :heart:


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Re: Psilocybe silvatica [Re: nomadbrad]
    #19508827 - 02/02/14 12:08 AM (9 years, 11 months ago)

Quote:

nomadbrad said:
:trippinbawelz::hotidea:

This is awesome news. Although I just toured 5 micro breweries this evening and tried 40+ beers I'm still stoked and coherent enough to understand the ramifications of not finding chrysostidia!

Joust thanks for not just dicking this specimen off as Hypholoma! Haha love you dude :heart:



Oh i wasnt about to, that was my initial thought, i knew i had more work to do, but one can only micro for so long, it takes a few micro sessions, usually easch session i try and change parameters of veiwing. I typically use Congo red stain or Phyloxen or whatever its called, I toured... my personal herbariaum of palnts tonight. :flyhigh: so im less coherant than you! woop. spellign, bet you guys didnt know im dyslexic, and im pretty sure it gets worse as i smoked pot. So yeah. now i just suck at math and backwards things and wirte wierd sometimes. But i digress....

This last time i used KOH and just used that,. Next time ill hydrate with some other stain and maybe use gsm again, mount differently for sure... but there will likely be a third micro if Alan has the time to chime in.


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: psylosymonreturns]
    #19509304 - 02/02/14 04:05 AM (9 years, 11 months ago)

Quote:

psylosymonreturns said:
Alan Where are you?





I just got back from a party in Berkeley.  I gave my Psilocybe talk earlier in the day in exchange for free admission, and later in the night I brought a microscope.  They set me up in a party bus next to a fortune teller lady.  So I got to hear all the peoples fortunes as I scoped spores from Mexico.  She was a really nice fortune teller and told everyone good things about their future.    Here is a pic from 30 minutes ago, after the fortune teller left...




I studied the type collection of Psilocybe silvatica two months ago and will look for my notes.


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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19510147 - 02/02/14 09:38 AM (9 years, 11 months ago)

Yes you did didn't you! This could get interesting!


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Re: Psilocybe silvatica [Re: psylosymonreturns]
    #19510663 - 02/02/14 11:47 AM (9 years, 11 months ago)

Nice scope!


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19510772 - 02/02/14 12:13 PM (9 years, 11 months ago)

Cool scope Alan! :popcorn:


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Re: Psilocybe silvatica [Re: Lepkaun]
    #19511969 - 02/02/14 04:28 PM (9 years, 11 months ago)

But Joust, those pics are stellar bud!! Nice job!


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Re: Psilocybe silvatica [Re: psylosymonreturns]
    #19512050 - 02/02/14 04:45 PM (9 years, 11 months ago)

Quote:

psylosymonreturns said:
But Joust, those pics are stellar bud!! Nice job!



:whathesaid:
These mircographs look amazing, far better than I expected :bow2: 
I'm sorry for being a dick about you not posting any a few days ago.
I can get a little irritable at times, I'm working on it.

Are you taking the pictures with you DSLR and then Photoshopping them?
The resolution looks huge compared to the ones that goes in my eye piece.

What did you use to rehydrate the mushrooms in the last set of pictures, is it just water?
Is that what a crush mount is supposed to look like?


--------------------
May you be filled with loving kindness.
May you be well.
May you be peaceful and at ease.
May you be happy.



AMU Q&A


Edited by maynardjameskeenan (02/02/14 04:47 PM)


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19513870 - 02/03/14 12:22 AM (9 years, 11 months ago)

Quote:

maynardjameskeenan said:
Quote:

psylosymonreturns said:
But Joust, those pics are stellar bud!! Nice job!



:whathesaid:
These mircographs look amazing, far better than I expected :bow2: 
I'm sorry for being a dick about you not posting any a few days ago.
I can get a little irritable at times, I'm working on it.

Are you taking the pictures with you DSLR and then Photoshopping them?
The resolution looks huge compared to the ones that goes in my eye piece.

What did you use to rehydrate the mushrooms in the last set of pictures, is it just water?
Is that what a crush mount is supposed to look like?




I will take a video of how i do things... its way to hard to show and i have a few tricks ive developed thanks to hearing what others do....


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

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Re: Psilocybe silvatica [Re: Joust]
    #19513874 - 02/03/14 12:24 AM (9 years, 11 months ago)

Quote:

Joust said:
Quote:

maynardjameskeenan said:
Quote:

psylosymonreturns said:
But Joust, those pics are stellar bud!! Nice job!



:whathesaid:
These mircographs look amazing, far better than I expected :bow2: 
I'm sorry for being a dick about you not posting any a few days ago.
I can get a little irritable at times, I'm working on it.

Are you taking the pictures with you DSLR and then Photoshopping them?
The resolution looks huge compared to the ones that goes in my eye piece.

What did you use to rehydrate the mushrooms in the last set of pictures, is it just water?
Is that what a crush mount is supposed to look like?




I will take a video of how i do things... its way to hard to show and i have a few tricks ive developed thanks to hearing what others do....



Its difficult to work with things while the time lapse is going, its done tonight or tomorrow...


RANDOM NEWS! I have a meeting with the president of the Pacific Northwest Key Council tomorrow morning to asses my collections! woo!


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19515678 - 02/03/14 12:39 PM (9 years, 11 months ago)

:smile:
Quote:

maynardjameskeenan said:
Quote:

Alonso CortesPerez said:
Notice how distant the stem is from the gills? All of them are like this too.
I think they may be a Deconica because they smell nothing like a Psilocybe, they smell kinda like old leaves.





Hello, I would like to study this collection if you agree. I want to know if it is a Psilocybe or Deconica.
Psilocybe caerulipes from Mexico, very little farinaceous smell and almost no blue stain. It is possible that it is a species of Psilocybe.
Great find!



I'll absolutely send some to you, it's an honor.
I think Alan gave me your address.
I'll send sample out to you and Joust before the end of the weekend. :smile:
Would 5 fruit bodies be enough?





Thank you very much maynardjameskeenan for the samples. For me it is also an honor to study these collections are very interesting. I hope soon to share with you mircographs of this collection.


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Re: Psilocybe silvatica [Re: Joust] * 1
    #19515688 - 02/03/14 12:41 PM (9 years, 11 months ago)

:smile:
Quote:

Joust said:
Quote:

Byrain said:
Ah shit, I thought that was labeled pleurocystidia, I should go to bed...

Yea, your pleurocystidia images look like basidioles.



thats what i thought tooo. check this out!

heres the 2000x CHEILOcystidia :lol::heart:












Joust Great Work!
I think it is not Psilocybe silvatica. The type of P. silvatica has cheilocystidia more similar to Those of P. caerulipes.
Saludos!


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Re: Psilocybe silvatica [Re: Alonso CortesPerez]
    #19517248 - 02/03/14 06:25 PM (9 years, 11 months ago)

Thanks Alonso!
Thank you for the input! i was doubtful it was Psilocybe silvatica. Interested to see if you find any pleurocystidia!
Good luck!


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19525294 - 02/05/14 12:46 PM (9 years, 11 months ago)

I am so confused as to what this is.  No one knows still?


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Re: Psilocybe silvatica [Re: kcrocker802]
    #19525989 - 02/05/14 03:07 PM (9 years, 11 months ago)

Quote:

kcrocker802 said:
I am so confused as to what this is.  No one knows still?





Probably a Deconica, however if there is chrysocystidia it is Hypholoma.


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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19526055 - 02/05/14 03:21 PM (9 years, 11 months ago)

It looks and seems like a Deconica to me, they are by far the biggest Deconica I've ever seen in person though...
Alan, do you know of a Deconica key that I can use?
The only ones that come up on Google aren't very good-
http://www.pnwherbaria.org/m/datasets/fungi/lists/pnw/strophariaceae.htm
http://www.svims.ca/council/Stroph_I.htm


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19526155 - 02/05/14 03:43 PM (9 years, 11 months ago)

Interesting.  I wish I could afford a microscope and get into all this stuff.  Gotta get back to school while I am still young and play with the scopes they have there.


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Re: Psilocybe silvatica [Re: kcrocker802]
    #19526340 - 02/05/14 04:12 PM (9 years, 11 months ago)

If you are in school you can always use their scopes.

Schools have all sorts of equipment. Just ask.


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Re: Psilocybe silvatica [Re: Ganzig]
    #19526581 - 02/05/14 04:57 PM (9 years, 11 months ago)

I plan on going back to school and studying at least some mycology mixed with simple bio, some ecology, and environmental education.  Also, take some political theory, and philosophy while I am at it.  Just getting residency in Washington for a price reduction right now.


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Re: Psilocybe silvatica [Re: kcrocker802]
    #19526919 - 02/05/14 06:15 PM (9 years, 11 months ago)

Good, i am glad we are moving away  from Psilocybe on this one. I really didnt think it was P silvatica.  :shrug:


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Re: Psilocybe silvatica [Re: psylosymonreturns]
    #19527038 - 02/05/14 06:41 PM (9 years, 11 months ago)

I think the habitat matches well for P. silvatica
-"It is gregarious but not cespitose on wood debris or on wood chips or in well-decayed conifer substratum or among fallen leaves of hardwoods from the end of September until December".

As does the macroscopic description
-"The cap is 0.8— 2(2.5) cm broad and is obtusely conic, becoming broadly campanulate to broadly conic, often with an acute umbo. It is tawny dark brown when moist, fading to pale yellowish brown or grayish brown. It is even to striatulate when moist, smooth, hygrophanous, and viscid when moist from a thin gelatinous pellicle that is barely separable, if at all. It sometimes has a grayish-green tinge along the margin. It fades to pale buff.
The gills are adnate to adnexed, close to subdistant, narrow to moderately broad, and dull grayish brown to cinnamon brown at first then smoky brown at maturity, edges remaining whitish.
The spores are dark purplish brown, (6.6)8.5 — 9.5(11) x (3.8)4 — 5.5(6) µm from 4-spored basidia, which are sometimes 2-spored.
The stipe is 2— 8 cm long by 1— 3 mm thick, equal to slightly enlarged at the base, tubular, and somewhat flexuous. It is pallid to brownish beneath a silky fibrillose covering. It becomes darker towards the base, partial veil poorly developed, cortinate, thin to obscure, and soon absent. It is slightly bluish-green at base.
It has a farinaceous taste and odor
".
besides the farinaceous taste and odor and the bluing base.

the microscopy is similar too 
-"Microscopic features: Pleurocystidia are absent. The cheilocystidia are 24 — 34(40) x 4.4 — 6.6(8.8) µm, fusoid ventricose to lageniform, with a long flexuous neck, and are1.6 — 2.2 µm thick."
The spore are close the right size, Pleurocystidia is absent,
cheilocystidia are a  slightly different shape.

Who knows, maybe Psilocybe silvatica is a misidentified Deconica, time will tell.
I am excited to see what Alonzo comes up with, he's basically a master in the genus


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19527413 - 02/05/14 07:52 PM (9 years, 11 months ago)

Quote:

maynardjameskeenan said:
cheilocystidia are a  slightly different shape.

Who knows, maybe Psilocybe silvatica is a misidentified Deconica, time will tell.
I am excited to see what Alonzo comes up with, he's basically a master in the genus





The cheilocystidia are actually a pretty different shape.  P. silvatica has cheilocystidia that are almost lanceolate.


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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19527502 - 02/05/14 08:08 PM (9 years, 11 months ago)

Something like that couldn't differ between collections, Phenotypes and what-not? I know sometimes macroscopic feature are different from one another.
I'm a total greenhorn when it comes to microscopy, forgive my ignorance.


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19527606 - 02/05/14 08:28 PM (9 years, 11 months ago)

Quote:

maynardjameskeenan said:
Something like that couldn't differ between collections, Phenotypes and what-not? I know sometimes macroscopic feature are different from one another.
I'm a total greenhorn when it comes to microscopy, forgive my ignorance.



they are pretty different you should look at alans picture of the cheilo on... i believe it was caerulipes, should have the same type of cystidia, i think thats what Alan said.


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19527656 - 02/05/14 08:39 PM (9 years, 11 months ago)

Wait so, the type collection for Psilocybe silvatica was collected in New York state and looks identical to P. caerulipes, microscopically?


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19527668 - 02/05/14 08:43 PM (9 years, 11 months ago)

Plot twist?


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Re: Psilocybe silvatica [Re: kcrocker802]
    #19527694 - 02/05/14 08:48 PM (9 years, 11 months ago)

Have there been confirmed (with microscopy) collections of P. silvatica in the PNW?


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19527752 - 02/05/14 08:59 PM (9 years, 11 months ago)

Is the description of Psilocybe silvatica on wikipedia from PMOTW or is it the original.  It isn't cited.


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Re: Psilocybe silvatica [Re: kcrocker802]
    #19527879 - 02/05/14 09:28 PM (9 years, 11 months ago)

It probably can from; Guzman, G. The Genus Psilocybe: or
Guzman, G., Jott, J. Boydston, S.H. Pollock. 1976 Psychotropic mycoflora of Washington, Idaho, Oregon, California and British Columbia

:shrug:
I'm just guessing, PMOTW doesn't list which species the "works cited" came from, it just rattles off references in no particular order alphabetically- by name of author.


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Edited by maynardjameskeenan (02/05/14 09:58 PM)


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19527994 - 02/05/14 09:52 PM (9 years, 11 months ago)

Quote:

maynardjameskeenan said:
It probably can from; Guzman, G. The Genus Psilocybe: or
Guzman, G., Jott, J. Boydston, S.H. Pollock. 1976 Psychotropic mycoflora of Washington, Idaho, Oregon, California and British Columbia

:shrug:
I'm just guessing, PMOTW doesn't list which species the "works cited" came from, it just rattles of references in no particular order.



I was talking to Fred Rhodes and he said that he finds pelliculosa in the deep forest. I have never seen them there, although i guess thats a thing. which ill investigate this next year. Maybe he found silvatica. There are many pelliculosa collections at WWB. Ill micro them at some point.


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Psilocybe silvatica [Re: Joust]
    #19528088 - 02/05/14 10:15 PM (9 years, 11 months ago)

Besides the collection that describes this mushroom, is there any other collections where someone can definitely say "this is Psilocybe silvatica"!? I know Alan said that there was a mix-up with it and Psilocybe pelliculosa in some lab, but most of the pictures I see online of the species looks more like pelliculosa, which you and I know for a fact- can varies greatly from place to place. Also the original collection being from New York state and looking just like Psilocybe caerulipes-microscopically sounds fishy to me. I can easily see the mushrooms that I found not be silvatica, but this seems like a convoluted mess.
-Forgive me for my dazed rant  :stoned2:


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19528297 - 02/05/14 11:17 PM (9 years, 11 months ago)

You might be on to something.  I remember reading somewhere maybe recent, but maybe in the archives that someone found that many collections of preserved Ps. pelliculosa were in fact Ps. silvatica.  The old range for Ps. caerulipes was definitely south of New York before it was sorted properly with Ps. ovoideocystidiata.


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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19529745 - 02/06/14 09:58 AM (9 years, 11 months ago)

Quote:

maynardjameskeenan said:
Besides the collection that describes this mushroom, is there any other collections where someone can definitely say "this is Psilocybe silvatica"!?





Yes, the collection that Jan sequenced came from Scott Redhead and was collected just north of Michigan.  The sequence data shows that it is different from P. caerulipes and P. pelliculosa.


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Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19530855 - 02/06/14 02:23 PM (9 years, 11 months ago)

Is there any good documentation of this find on the internet? On this site or elsewhere?  I would love to see pictures.  So no one is aware of P. silvatica being confirmed on the West Coast?


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Deconica? [Re: maynardjameskeenan]
    #19916461 - 04/29/14 11:41 AM (9 years, 8 months ago)

Tenía mucho trabajo pendiente y por fin pude estudiar esta colección con mucho cuidado. Muchas gracias a Maynardjameskeenan.
Busqué por mucho tiempo y solo pude encontrar pleurocystidia en tres basidiomas, los demás basidomas no tienen este tipo de pleurocystidia.
Creo que tal vez es una especie de Deconica, tal vez análisis de ADN ayuden para saber si pertenece a Deconica.

pleurocystidia like Chrysocystidia?



Pleurocystidia



Pleurocystidia


Pleuro


Pleuro


pleuro


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Re: Deconica? [Re: Alonso CortesPerez] * 1
    #19916578 - 04/29/14 12:12 PM (9 years, 8 months ago)

Nice micro! I assume you are THE Alonso, so I would expect nothing less. I haven't been following this thread, but I think I'll back track and have a looksy!


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Re: Deconica? [Re: Alonso CortesPerez]
    #19916664 - 04/29/14 12:32 PM (9 years, 8 months ago)

Quote:

Alonso CortesPerez said:
Tenía mucho trabajo pendiente y por fin pude estudiar esta colección con mucho cuidado. Muchas gracias a Maynardjameskeenan.
Busqué por mucho tiempo y solo pude encontrar pleurocystidia en tres basidiomas, los demás basidomas no tienen este tipo de pleurocystidia.
Creo que tal vez es una especie de Deconica, tal vez análisis de ADN ayuden para saber si pertenece a Deconica.

pleurocystidia like Chrysocystidia?



Pleurocystidia



Pleurocystidia


Pleuro


Pleuro


pleuro



You're amazing! Muchas gracias :hatsoff:


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Re: Deconica? [Re: maynardjameskeenan]
    #19916685 - 04/29/14 12:39 PM (9 years, 8 months ago)

But it looked blue :frown:


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Deconica? [Re: Joust]
    #19917188 - 04/29/14 02:48 PM (9 years, 8 months ago)

Monta en agua o hidroxido de potasio?

La forma de las esporas no es muy subrhomboid, hay muchas especies Deconica con esa forma de esporas?

Creo que con chrysocystidia y que forma esporas, Hypholoma es muy probable.

Quote:

Joust said:
But it looked blue :frown:




No vi ningun azul en el OP.


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Re: Deconica? [Re: Alan Rockefeller]
    #19917499 - 04/29/14 04:05 PM (9 years, 8 months ago)

Cuarta imagen hacia abajo en la base?


must not be, eyes....:shrug:


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_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Re: Deconica? [Re: Joust]
    #19917557 - 04/29/14 04:23 PM (9 years, 8 months ago)

Quote:

Joust said:
Cuarta imagen hacia abajo en la base?






No lo veo azul alli.


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Re: Deconica? [Re: Alan Rockefeller]
    #19917660 - 04/29/14 04:51 PM (9 years, 8 months ago)

mi casa o en la suya, el SIDA para la vida que es tu decisión.
:trollhide:


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Offlinenomadbrad
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Re: Deconica? [Re: maynardjameskeenan]
    #19919018 - 04/29/14 09:27 PM (9 years, 8 months ago)

Can somebody help me translate this?  I've been wondering for several months what I found.


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Offlineraceme
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Re: Deconica? [Re: nomadbrad]
    #19919063 - 04/29/14 09:42 PM (9 years, 8 months ago)

Quote:

Alonso CortesPerez said:
I had a lot of work to get through, but I finally was able to  study this collection carefully.  Thank you MaynardJamesKeenan.  After mush searching I was only able to find pleurocystidia in three of the basidiocarps, and none of the rest had this type of pleurocystidia.  I believe it is a species of Deconica, and DNA analysis would help to tell is it belongs there.






Quote:

Alan Rockefeller said:

Did you mount it in water, or potassium hydroxide?

The shape of the spores isn't very subrhomboid, are there many species of Deconica with similarly shaped spores?

I think that, given the chrysocystidia and the shape of the spores, Hypholoma is more likely.
Quote:

Joust said:
But it looked blue :frown:




I didn't see any blue in the el OP.




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InvisibleJoust
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Re: Deconica? [Re: raceme] * 1
    #19919457 - 04/29/14 11:35 PM (9 years, 8 months ago)

Hypholoma! that seems to fit should dig around these collections next time, there are a few really cool sclerotia forming species in Strophariaceae...


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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OfflineAlan RockefellerM
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Re: Deconica? [Re: nomadbrad]
    #19920190 - 04/30/14 03:14 AM (9 years, 8 months ago)

Quote:

nomadbrad said:
Can somebody help me translate this?





You should probably just learn spanish, we are going to be speaking a lot of it in the next few years because it just so happens that the countries that have the most interesting mushrooms tend to have spanish as the official language.

I don't know why this is, appears that spain conquered pretty much everything south of texas....so....


I can go mushroom hunting in California and find one really interesting collection per year, or go mushroom hunting in southern Mexico and find one really interesting collection per day.  I know spanish is hard, I struggle with it every day....but...it does work very well and it is a really fun language.


The best way to learn is to copy/paste into translate.google.com.  Another really fun and educational thing to do is edit the spanish wikipedia.  A lot of the mushroom articles there need help : )



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InvisibleHunter hunter
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Re: Deconica? [Re: Alan Rockefeller]
    #19921194 - 04/30/14 10:58 AM (9 years, 8 months ago)

Wow.  I'm glad I found this post today.  I hate waiting around for the answer. 


Friggin cool find Maynard.  Those would have stumped a noob.  If I found those in the past I would have lemon teked .5 of a gram to check for any activity.  I know that's dumb but that's how I learned.


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    Eat the meat that’s at your feet.


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Invisiblemaynardjameskeenan
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Re: Deconica? [Re: Hunter hunter]
    #19921652 - 04/30/14 12:51 PM (9 years, 8 months ago)

https://www.duolingo.com/

IOS
Android
It's free, the setup and UI are really good too.

Learning a second language is really good for your brain and improves cognition.
http://www.spring.org.uk/2013/09/10-superb-psychological-advantages-of-learning-another-language.php


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AMU Q&A


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OfflineAlan RockefellerM
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Re: Deconica? [Re: maynardjameskeenan]
    #25322813 - 07/12/18 06:19 PM (5 years, 6 months ago)

Today I got a DNA sequence from this collection and it matches 100% with Hypholoma marginatum.

Congratuations to Inski for first guessing the genus and to psylosymonreturns for being the first to mention the species.

Sequence: https://mushroomobserver.org/sequence/show_sequence/1371


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