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Offlineblueconfusion
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: blueconfusion]
    #18999571 - 10/19/13 12:34 PM (10 years, 4 months ago)

Just a for instance


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Offlinegreenery1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: blueconfusion]
    #19000471 - 10/19/13 04:32 PM (10 years, 4 months ago)

I was hoping someone here could tell me that! :smile:


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Offlineblueconfusion
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19000659 - 10/19/13 05:24 PM (10 years, 4 months ago)

knock up some brf or grain...:shrug:


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InvisibleEnigma1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: blueconfusion]
    #19001495 - 10/19/13 08:53 PM (10 years, 4 months ago)

Quit being scared and test it . If it's contaminated it's contaminated.


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Offlinegreenery1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Enigma1]
    #19002887 - 10/20/13 07:25 AM (10 years, 4 months ago)

need to get vermiculite, local hydroponics only sells it in huge amounts...


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Offlineblueconfusion
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19002969 - 10/20/13 08:07 AM (10 years, 4 months ago)

i buy huge bags http://www.menards.com/main/building-materials/insulation/loose-fill/vermiculite-insulation/p-1485908-c-5777.htm here it's actually the same stuff nothing nasty and costs less than if you were to buy it in gardening section...i compared:dancer:


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Offlinegreenery1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: blueconfusion]
    #19003008 - 10/20/13 08:28 AM (10 years, 4 months ago)

Hmm thats actually about the same size, but here it is 40USD. They also have perlite the same size, said they would give me half of each if I wanted. I guess you do go through it pretty fast so maybe I'll just go for that.


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Offlineblueconfusion
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19003037 - 10/20/13 08:43 AM (10 years, 4 months ago)

like i said $40 at the hydro store (everything will be marked up there) or $14 in the building materials section...just gotta do a little looking is all :shrug:


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Offlineskippydude
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19003084 - 10/20/13 09:03 AM (10 years, 4 months ago)

Quote:

greenery1 said:
ah...I wasn't aware that mycilium could be contaminated even if it is growing.



LCs can carry contamination even if they are fluffy white myc growing in a crystal clear solution and must be  grown out to test.

I've had LCs that look like yours from carmelization of the sugars in the PC that grew clean  and I've had them that look like yours that were dirty
Even with a microscope contamination may not be visible. I've had bacteria stowe away within the mycelium, where it  grew clean looking on agar and spoiled every jar of grain I put it in.


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Offlineblueconfusion
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19003125 - 10/20/13 09:20 AM (10 years, 4 months ago)

just to reiterate LCs are flawed especially when doing MS with a cube.  if you want more spawn do G2G transfers it's a hard lesson to learn but there is not as much guessing, or if you can do MS to agar which is even better do that.  LCs in theory are great and very appealing but if you don't have your technique down i wouldn't recommend that, cause now really all you can do is grow out what you have and hope for the best...


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Offlinegreenery1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: blueconfusion]
    #19004318 - 10/20/13 03:01 PM (10 years, 4 months ago)

I was originally going to do MS to agar instead of LC, but I was reading that agar is for strain isolation, and LC would be better if you just wanted to give your BRF a jump start when inoculating. I see now that is just as risky as just knocking up the BRF directly?

After BRF i was going to go to casing, my first go at it so I'm just using a pot at the moment to sterilize the LC and BRF cakes. If successful will invest in a PC, but if unsuccessful, um, will also invest in a PC... :smile:


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Offlineskippydude
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19004634 - 10/20/13 04:16 PM (10 years, 4 months ago)

If you made LC without a PC, it's not very likely to be clean :frown:
Not having a PC kinda restricts you to the PF tek using MS syringes made from spore prints.

If you wish to go further in the hobby you'll need about a 20q PC
It's a $100 investment that you will never regret, I wore out my $100 Presto and picked up a $325 AA. I know it's wrong to have feelings for inanimate objects, but I still get a little chubber when using that "All American 930"


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Offlinegreenery1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19004683 - 10/20/13 04:28 PM (10 years, 4 months ago)

ah i see, thanks. I used a rice cooker (giving about 15 psi?). i was sure I read that LC could also be done that way, along with PF.


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OfflineISuckAtHalo
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19004697 - 10/20/13 04:31 PM (10 years, 4 months ago)

Quote:

skippydude said:
, but I still get a little chubber when using that "All American 930"



:whatwhatwhat:


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InvisiblePestile

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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19004728 - 10/20/13 04:40 PM (10 years, 4 months ago)

Quote:

skippydude said:
If you made LC without a PC, it's not very likely to be clean :frown:





Actually, the ingredients we use for LC are not filled with different types of endospores and such, so we don't have to pressure cook liquid cultures. A simple 30 min. boil will do. :smile:


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Offlineskippydude
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19005041 - 10/20/13 06:01 PM (10 years, 4 months ago)

Quote:

greenery1 said:
ah i see, thanks. I used a rice cooker (giving about 15 psi?). i was sure I read that LC could also be done that way, along with PF.



If you are at 15 psi then you are indeed pressure cooking :thumbup:


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Offlineskippydude
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Pestile]
    #19005075 - 10/20/13 06:06 PM (10 years, 4 months ago)

Quote:

Pestilence said:
Quote:

skippydude said:
If you made LC without a PC, it's not very likely to be clean :frown:





Actually, the ingredients we use for LC are not filled with different types of endospores and such, so we don't have to pressure cook liquid cultures. A simple 30 min. boil will do. :smile:



If ingredients are exposed to air at anytime during the process, prior to sterilization, then they have been exposed to endospores.


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InvisiblePestile

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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19005161 - 10/20/13 06:23 PM (10 years, 4 months ago)

The point is that there is no grain core that needs to be heated up so pressure is not needed to sterilize the LC.
I could find a RR quote somewhere, but I really don't want to. :smile:


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Offlineskippydude
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Pestile]
    #19005349 - 10/20/13 06:56 PM (10 years, 4 months ago)

An endospore has a hard outer shell on it that requires 251F to penetrate wether it is in, on or around anything, grain has nothing to do with it.
These things survive space travel and boiling water won't kill all of them.
If your not having issues I'd say are working in a pretty sanitary environment and have been lucky.


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InvisiblePestile

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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19007111 - 10/21/13 04:39 AM (10 years, 4 months ago)

Quote:

skippydude said:
An endospore has a hard outer shell on it that requires 251F to penetrate wether it is in, on or around anything, grain has nothing to do with it.
These things survive space travel and boiling water won't kill all of them.
If your not having issues I'd say are working in a pretty sanitary environment and have been lucky.




You are right. I had some info mixed up, sorry about that. :smile:


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