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Offlineitsaconspiracy
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Shiitake grow part.2 sad update:(
    #18960928 - 10/10/13 06:56 PM (10 years, 4 months ago)

o.k the first grow didn't go so well because of a contaminated syringe ,but hopefully this one goes better.

here is the syringe in question that was used now empty


cakes in my SAB before inoculation

and cakes after inoculation


All cakes are in the traditional PF cake style hope i see some growth soon:bigyesnod:


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Edited by itsaconspiracy (10/18/13 05:18 PM)


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Offlineakalais
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Re: Shiitake grow part.2 [Re: itsaconspiracy]
    #18963695 - 10/11/13 12:09 PM (10 years, 4 months ago)

Cool glovebox.

Keep us updated!


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Offlineitsaconspiracy
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Re: Shiitake grow part.2 [Re: akalais]
    #18973252 - 10/13/13 05:48 PM (10 years, 4 months ago)

so far no signs of growth unlike last time the contam started to show its ugly head around days 2-4 so  if i don't see by day five i might be safe oh ya the LC was sent as a replacement for the last one by TSD.


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Offlineitsaconspiracy
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Re: Shiitake grow part.2 [Re: itsaconspiracy]
    #18996535 - 10/18/13 05:24 PM (10 years, 4 months ago)

well a sad update there contaminated again with the same blue green fungus here's some pics



but damn it i ain't giving up on the shiitake i eventually succeed just I'm looking for a print or a syringe from a sponser instead of a lc cause so far liquid cultures suck:mad:


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Invisibleliamtheloser
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Re: Shiitake grow part.2 [Re: itsaconspiracy]
    #18996627 - 10/18/13 05:39 PM (10 years, 4 months ago)

Yeah,  get a strain from someone else.  I got a contaminated culture from TSD too.  Seems sloppy to me even though they sent me a replacement.

I have Shiitake on agar if you feel comfortable doing agar work


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Offlineitsaconspiracy
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Re: Shiitake grow part.2 [Re: liamtheloser]
    #18996684 - 10/18/13 05:55 PM (10 years, 4 months ago)

Quote:

I have Shiitake on agar if you feel comfortable doing agar work



sadly I think i'm too much of a noob to mess with agar:sad:

Quote:

liamtheloser said: I got a contaminated culture from TSD too.  Seems sloppy to me even though they sent me a replacement.





and this is the replacement for the last culture they sent me and just like the last one it contaminated with the same type of fungus.


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OfflineForresterM
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Re: Shiitake grow part.2 [Re: itsaconspiracy]
    #18996956 - 10/18/13 07:10 PM (10 years, 4 months ago)

If you can buy a plastic tote and cut two half-circles in it, you can mess with agar.  I fought it for so long and in the end everyone was right, it's really not that hard.  Yeah you'll have some contamination with a shitty still air box but more often than not it works out fine.

Especially if you do like I did and use those little flat square-ish mason jars instead of petris.  Then you don't even need para-film.  I've had very few problems since I switched to those.


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlineitsaconspiracy
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Re: Shiitake grow part.2 [Re: Forrester]
    #18996992 - 10/18/13 07:16 PM (10 years, 4 months ago)

:hmm: o.k can you show me a pic of the mason jars and I'll take a look for them when i get to work. and I'll give it a try why not worst case scenario is another contam.


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OfflineForresterM
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Re: Shiitake grow part.2 [Re: itsaconspiracy]
    #18997085 - 10/18/13 07:34 PM (10 years, 4 months ago)



Most Wal-marts should have 'em, (made by Ball but I can't remember for the life of me what they're called) and you can see on the lid (wide-mouth for scale) all I use is a 1/4" or so hole with a filter patch siliconed in. 
I'm no agar expert just a noob like you and I've done really well with these.  Tape over the hole and PC with the agar in it so no need to pour agar inside a SAB and risk contam there.  Just pull off the tape while it's still hot for minimal condensation (you still get some, just pour it out inside your SAB before you throw your wedge in).


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlineitsaconspiracy
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Re: Shiitake grow part.2 [Re: Forrester]
    #18997100 - 10/18/13 07:37 PM (10 years, 4 months ago)

cool thanks Forrester I'll look(work at a wal-mart) I'll find them if their in there:yesnod:


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OfflineForresterM
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Re: Shiitake grow part.2 [Re: itsaconspiracy]
    #18997198 - 10/18/13 07:57 PM (10 years, 4 months ago)

Sweet you already got the silicon there then too :thumbup:

Agar is kinda expensive but a little goes a long way when you're working small scale ($20 or so should get you all you need for quite a while, I got from out-grow).  Malt extract is cheap and you need very little.  Grab a scalpel handle and 100 sterile blades on amazon for like $14 and that's pretty much all you need.  Like I said I'm not one of the experts but I can help ya with what works for me (technique wise and such), being pretty new at it as well, if you decide to go the agar route.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinedeadmandave
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Re: Shiitake grow part.2 [Re: Forrester]
    #18998702 - 10/19/13 07:34 AM (10 years, 4 months ago)

hey Forrester, I have seen those jars and they are tempting...
Do you use a filter disc under that hole? Is the hole only to help with condensation?

I buy agar agar powder at the asain mart for 1.50 for 25 grams. For 1 liter of water I use 20 grams agar, add 10 grams light malt extract, teaspoon of yeast and the mushrooms love it :biggrin: 

Someday i will try potato water agar.


Edited by deadmandave (10/19/13 07:35 AM)


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OfflineForresterM
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Re: Shiitake grow part.2 [Re: deadmandave]
    #18998758 - 10/19/13 08:20 AM (10 years, 4 months ago)

I just cut a little square out of a filter disc and silicon in under, that way I can make 4 lids out of each filter disc.  Hole is mainly for gas exchange and yeah it helps let some of the condensation out while its cooling.

I so wish we had an asian market around here!  Good point, that's definitely the place to go for agar if you have access to one.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineJeff
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Re: Shiitake grow part.2 [Re: Forrester]
    #18998792 - 10/19/13 08:40 AM (10 years, 4 months ago)

Just make sure it 100% agar.  Some of the stuff in Asian markets have preservatives.


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Invisibleliamtheloser
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Re: Shiitake grow part.2 [Re: Jeff]
    #18999625 - 10/19/13 12:47 PM (10 years, 4 months ago)

Telephone brand agar is 100% agar, no sugar, no sulphites.

I get mine from asian market or vitamin store.  NOW foods makes agar that's pretty affordable.  I bought pretty much a lifetime supply a while back for 20 dollars.  It's available cheap if you know where to look :wink:

I bought 100 polypropylene plates for 20 bucks, that should last me a while, I premix and PC them too since I don't have a flow hood.  I also don't buy the normal nutrients, plenty of crap in your cupboards at home that will serve as nutrients for your agar.  AKA corn syrup, molasses, sawdust, nutrient yeast, yeast, potato flakes, etc, etc, etc.


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Offlineitsaconspiracy
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Re: Shiitake grow part.2 [Re: Forrester]
    #19000834 - 10/19/13 06:11 PM (10 years, 4 months ago)

Quote:

Forrester said:
Sweet you already got the silicon there then too :thumbup:

Agar is kinda expensive but a little goes a long way when you're working small scale ($20 or so should get you all you need for quite a while, I got from out-grow).  Malt extract is cheap and you need very little.  Grab a scalpel handle and 100 sterile blades on amazon for like $14 and that's pretty much all you need.  Like I said I'm not one of the experts but I can help ya with what works for me (technique wise and such), being pretty new at it as well, if you decide to go the agar route.




ya i got four of the jars(because that all they had ,but soupost to get more on Thursday:thumbup:) and thanks for all the info if i have anymore question i will definitely ask:bigyesnod: thank for being so helpful.

Quote:

liamtheloser said:
Telephone brand agar is 100% agar, no sugar, no sulphates.

I get mine from asian market or vitamin store.  NOW foods makes agar that's pretty affordable.  I bought pretty much a lifetime supply a while back for 20 dollars.  It's available cheap if you know where to look

I bought 100 polypropylene plates for 20 bucks, that should last me a while, I premix and PC them too since I don't have a flow hood.  I also don't buy the normal nutrients, plenty of crap in your cupboards at home that will serve as nutrients for your agar.  AKA corn syrup, molasses, sawdust, nutrient yeast, yeast, potato flakes, etc, etc, etc.






i look in the asian section of wal-mart and i didn't see any agar and i looked in the vegetarian section too ,but still no agar:shrug:


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Invisibleliamtheloser
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Re: Shiitake grow part.2 [Re: itsaconspiracy]
    #19002063 - 10/19/13 11:14 PM (10 years, 4 months ago)

You wont find agar at walmart, you'd have to go to an actual asian grocery store.


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Offlineitsaconspiracy
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Re: Shiitake grow part.2 [Re: liamtheloser]
    #19004831 - 10/20/13 05:04 PM (10 years, 4 months ago)

humm i'll try and find one.


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Re: Shiitake grow part.2 [Re: itsaconspiracy]
    #19006092 - 10/20/13 09:43 PM (10 years, 4 months ago)



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Offlineitsaconspiracy
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Re: Shiitake grow part.2 [Re: CAP_TURTLE]
    #19009602 - 10/21/13 05:35 PM (10 years, 4 months ago)

heck ya 4 bucks plus shipping is hard to beat:awesomenod:


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