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InvisibleMUSH HEAD420
Kush Commander
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Registered: 09/14/08
Posts: 921
Gymnopilus Mycelium
    #18997222 - 10/18/13 08:01 PM (10 years, 4 months ago)

I inoculated some wbs with some gym spores from a recent find (in my sig). My question is, I have noticed growth at 5 days around the sterilized cotton swabs I used to collect the spores off the glass plate with.

I did all this as sterile as possible, the only contam risk was the spore prints and yes I understand that was a big one but I used a large quantity of spore prints in hope that the mycelium  would overtake any contams originating on the swab.

I do not have a good enough cam to take pics of the growth as it is small, so I will describe it for now until it takes off.

The growth starts right around all four swab locations, on the grain right next to the swab I can see what looks like very small fluffy white dots that sort of merge and out from that is white fibers about .05-.1 mm thick, like realy thin coten fibers, radiating 1 inch from the swab. Going from grain to grain.

Thank you for any input : )


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Offlinedeadmandave
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Registered: 02/16/10
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Re: Gymnopilus Mycelium [Re: MUSH HEAD420]
    #18998710 - 10/19/13 07:40 AM (10 years, 4 months ago)

cant tell you what you are growing, hopefully it's gymnopilus. what i do know is that when you are taking spores from a print its a good idea to take a small sample size, this way you decrease the chance of picking up contam spores as well.

as you look at a print you will see the spores, it is thousands of spores stacked up allowing you to see them, so really there is no need to swipe up a big portion of the print. A small swipe with an inoculation loop is generally all that is necessary.


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InvisibleMUSH HEAD420
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Registered: 09/14/08
Posts: 921
Re: Gymnopilus Mycelium [Re: deadmandave]
    #18999128 - 10/19/13 10:40 AM (10 years, 4 months ago)

If it is gym myc can I just sit around and wait for a contam to show, then take a piece of myc without contam and start a new jar with it?

If this jar does not contam I will shit bricks.. lol

I do not plan to have agar and dishes for at least a month, and I cant wait that long, at least by the time I have my agar and dishes I may have some live myc to throw in the incubator.


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