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mellowparty
legitimate researcher


Registered: 05/17/09
Posts: 18,467
Last seen: 10 years, 2 months
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I need a molecular biologist
#18998343 - 10/19/13 02:00 AM (10 years, 3 months ago) |
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Yeah yeah I know its not pubscum topic but le pube owns other boards in terms of traffic.
Anyway my question is about protein blotting. Y'all know what western blotting does but my retarded supervisor said there is no eastern blotting. He said that because he has read like 2-3 textbooks for our lectures and doesn't mention it cause its not mentioned there (he's a real dick too)
Anyway if eastern blots are used to detect post-translational modifications like acetylation, acylation (myristoylation, palmitoylation), alkylation, arginylation, ADP-Ribosylation, biotinylation, formylation, geranylgeranylation, glutamylation, glycosylation, glycylation, hydroxylation, isoprenylation, lipoylation, methylation, nitroalkylation, phosphopantetheinylation, phosphorylation, prenylation, selenation, S-nitrosylation, succinylation, sulfation, transglutamination, ubiquitination and so.
Wouldn't that make western blotting with anti-phosphosite antibodies essentially an eastern blot and not technically a western blot?
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Sophistic Radiance
Free sVs!



Registered: 07/11/06
Posts: 43,135
Loc: Center of the Universe
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Re: I need a molecular biologist [Re: mellowparty]
#18998345 - 10/19/13 02:03 AM (10 years, 3 months ago) |
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Quote:
mellowparty said: Yeah yeah I know its not pubscum topic but le pube owns other boards in terms of traffic.
Anyway my question is about protein blotting. Y'all know what western blotting does but my retarded supervisor said there is no eastern blotting. He said that because he has read like 2-3 textbooks for our lectures and doesn't mention it cause its not mentioned there (he's a real dick too)
Anyway if eastern blots are used to detect post-translational modifications like acetylation, acylation (myristoylation, palmitoylation), alkylation, arginylation, ADP-Ribosylation, biotinylation, formylation, geranylgeranylation, glutamylation, glycosylation, glycylation, hydroxylation, isoprenylation, lipoylation, methylation, nitroalkylation, phosphopantetheinylation, phosphorylation, prenylation, selenation, S-nitrosylation, succinylation, sulfation, transglutamination, ubiquitination and so.
Wouldn't that make western blotting with anti-phosphosite antibodies essentially an eastern blot and not technically a western blot?
Don't western blots just read out the concentration of polypeptides within a certain length range? I suppose you could derive the same information from a western as from an eastern, but only under the right circumstances.
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mellowparty
legitimate researcher


Registered: 05/17/09
Posts: 18,467
Last seen: 10 years, 2 months
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You could do that with a western yes if you integrate the pixels in terms of a 16 bit grayscale with respect to the membrane surface after development. The thing is I've used antiphospho antibodies and always called it a western blot, now I feel like I've been deluding myself with the nomenclature of these teks.
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Le_Canard
The Duk Abides


Registered: 05/16/03
Posts: 94,392
Loc: Earthfarm 1
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Re: I need a molecular biologist [Re: mellowparty] 1
#18998361 - 10/19/13 02:15 AM (10 years, 3 months ago) |
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I stay up all night sometimes worrying about these blots. I CURSE THEE, BLOTS!
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mellowparty
legitimate researcher


Registered: 05/17/09
Posts: 18,467
Last seen: 10 years, 2 months
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Re: I need a molecular biologist [Re: Le_Canard] 1
#18998367 - 10/19/13 02:18 AM (10 years, 3 months ago) |
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Are you worried about blotting proteins on your underwear cause yeah that happens sometimes
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Le_Canard
The Duk Abides


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Posts: 94,392
Loc: Earthfarm 1
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Re: I need a molecular biologist [Re: mellowparty] 1
#18998373 - 10/19/13 02:21 AM (10 years, 3 months ago) |
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I just did that. Mission accomplished.
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Sophistic Radiance
Free sVs!



Registered: 07/11/06
Posts: 43,135
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Re: I need a molecular biologist [Re: mellowparty]
#19001669 - 10/19/13 09:38 PM (10 years, 3 months ago) |
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Quote:
mellowparty said: Are you worried about blotting proteins on your underwear cause yeah that happens sometimes 
That is a "southern blot".
-------------------- Enlil said: You really are the worst kind of person.
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mellowparty
legitimate researcher


Registered: 05/17/09
Posts: 18,467
Last seen: 10 years, 2 months
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that could be an inside a joke.
"What did you do last night?" "I did a southern in my pants"
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