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lonelyjew
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Registered: 05/02/13
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Reinoculate forgot to shake syringe?
#18996940 - 10/18/13 07:08 PM (10 years, 4 months ago) |
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alright so sort of panicking i did everything by the book inoculated in a SAB but i forgot to shake the syringe?? should i reinoculate or will this increase risk of contams by too much?
i used about a cc per pint jar (.5cc for 6 of them then reinoculated them with another .5cc and the other 6 i just put in a full 1-1.5cc)
what do you guys think!!!
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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Eveniftheskyfalls
Even if the sky falls



Registered: 09/08/13
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Re: Reinoculate forgot to shake syringe? [Re: lonelyjew]
#18997022 - 10/18/13 07:21 PM (10 years, 4 months ago) |
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Im just a noob but i think your fine, the spores are microscopic.I inoculated 10 jars and 5 of which i didnt shake the syringe.and theyre all growing healthy mycelium
-------------------- Sanity and Happiness are an impossible combination. Even when the sky falls, Ill still be high Anything illegal or has to do with mushrooms that i talk about is completely 100% fictional and is not true. I dont even visit this site. ( I am a proud provider and receiver of positive vibes ) "Reading a trip report is funny. Its like reading a book. You get to visualize the words... Only the visualization is always of some dude starring at the ceiling for a few hours"- Eveniftheskyfalls Progress in life since I found this site (look below)
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lonelyjew
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thats a little comforting still an experienced opinion is welcome.
should i reinoculate just in case or is contam to risky?
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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SynKyd
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Registered: 09/27/13
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Re: Reinoculate forgot to shake syringe? [Re: lonelyjew]
#18997139 - 10/18/13 07:45 PM (10 years, 4 months ago) |
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you should be fine. I didn't shake mine much and in 30 jars through 5 syringes over 3 separate innoculations and I have 30 for 30 growth with zero contams so far.
-------------------- New inclusive poop emojis from Apple!
   
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Eveniftheskyfalls
Even if the sky falls



Registered: 09/08/13
Posts: 449
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Re: Reinoculate forgot to shake syringe? [Re: lonelyjew]
#18997147 - 10/18/13 07:47 PM (10 years, 4 months ago) |
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Personally, I would wait a day or two to see if it starts to grow mycelium, And if it doesnt then either restart and make new jars or steam sterilize again. Or pressure cook. Depending on what you have. Good luck!
-------------------- Sanity and Happiness are an impossible combination. Even when the sky falls, Ill still be high Anything illegal or has to do with mushrooms that i talk about is completely 100% fictional and is not true. I dont even visit this site. ( I am a proud provider and receiver of positive vibes ) "Reading a trip report is funny. Its like reading a book. You get to visualize the words... Only the visualization is always of some dude starring at the ceiling for a few hours"- Eveniftheskyfalls Progress in life since I found this site (look below)
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lonelyjew
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Re: Reinoculate forgot to shake syringe? [Re: SynKyd]
#18997154 - 10/18/13 07:48 PM (10 years, 4 months ago) |
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alright thats a relief how long till i should see first signs of growth? temperature is at a minimum of 75 I'll try and keep it at 80 and if its been a week and no signs of growth should i reinoculate if i see no contams?
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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lonelyjew
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Re: Reinoculate forgot to shake syringe? [Re: lonelyjew]
#18997161 - 10/18/13 07:49 PM (10 years, 4 months ago) |
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alright thanks i'll give it 3 days to be safe and just PC them again
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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Eveniftheskyfalls
Even if the sky falls



Registered: 09/08/13
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Re: Reinoculate forgot to shake syringe? [Re: lonelyjew]
#18997174 - 10/18/13 07:52 PM (10 years, 4 months ago) |
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Well i saw growth after about 2 days but i heard it can take up to two weeks to see any signs.If you have a magnifying loop, you can look at the glass and see little tiny white hairs. I did atleast, but I would deff re-sterilize them atleast
-------------------- Sanity and Happiness are an impossible combination. Even when the sky falls, Ill still be high Anything illegal or has to do with mushrooms that i talk about is completely 100% fictional and is not true. I dont even visit this site. ( I am a proud provider and receiver of positive vibes ) "Reading a trip report is funny. Its like reading a book. You get to visualize the words... Only the visualization is always of some dude starring at the ceiling for a few hours"- Eveniftheskyfalls Progress in life since I found this site (look below)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Quote:
Eveniftheskyfalls said: Well i saw growth after about 2 days but i heard it can take up to two weeks to see any signs.If you have a magnifying loop, you can look at the glass and see little tiny white hairs. I did atleast, but I would deff re-sterilize them atleast
If I saw growth after 2 days I would suspect contams The fastest germination I ever saw was on agar and took 4 days from a really fresh print. OP if you don't see anything after 2 weeks you should start over.
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lonelyjew
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Re: Reinoculate forgot to shake syringe? [Re: Pastywhyte]
#18997224 - 10/18/13 08:02 PM (10 years, 4 months ago) |
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alright then thanks for all the help
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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Eveniftheskyfalls
Even if the sky falls



Registered: 09/08/13
Posts: 449
Last seen: 8 years, 1 month
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Re: Reinoculate forgot to shake syringe? [Re: Pastywhyte]
#18997228 - 10/18/13 08:03 PM (10 years, 4 months ago) |
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Quote:
Pastywhyte said:
Quote:
Eveniftheskyfalls said: Well i saw growth after about 2 days but i heard it can take up to two weeks to see any signs.If you have a magnifying loop, you can look at the glass and see little tiny white hairs. I did atleast, but I would deff re-sterilize them atleast
If I saw growth after 2 days I would suspect contams The fastest germination I ever saw was on agar and took 4 days from a really fresh print. OP if you don't see anything after 2 weeks you should start over.
I used a syringe that had x4 the amount of spores in it, i also inoculated my jars right after cooking them, which was a very noob move but yeah. I saw little tiny white hairs with a magnifying loop after 2 days
-------------------- Sanity and Happiness are an impossible combination. Even when the sky falls, Ill still be high Anything illegal or has to do with mushrooms that i talk about is completely 100% fictional and is not true. I dont even visit this site. ( I am a proud provider and receiver of positive vibes ) "Reading a trip report is funny. Its like reading a book. You get to visualize the words... Only the visualization is always of some dude starring at the ceiling for a few hours"- Eveniftheskyfalls Progress in life since I found this site (look below)
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lonelyjew
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also is it safe to handle them without gloves or should i really wear gloves when handling them, also do they need to be in a dark place during colonization?
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Reinoculate forgot to shake syringe? [Re: lonelyjew]
#18997270 - 10/18/13 08:13 PM (10 years, 4 months ago) |
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Quote:
Eveniftheskyfalls said: I used a syringe that had x4 the amount of spores in it, i also inoculated my jars right after cooking them, which was a very noob move but yeah. I saw little tiny white hairs with a magnifying loop after 2 days
More spores is not necessarily better Many molds have tiny white hairs I'm sure if OP could take a pic through a loop you could solve this for us. Not saying it didn't happen but it sure isn't the norm 
Quote:
lonelyjew said: also is it safe to handle them without gloves or should i really wear gloves when handling them, also do they need to be in a dark place during colonization?
You don't need gloves but you should not be handling them much either. You don't want to disturb the dry verm layer so don't touch them anymore than is absolutely necessary. Ambient light on a 12/12 cycle is beneficial at all stages.
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lonelyjew
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Last seen: 3 years, 2 months
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Re: Reinoculate forgot to shake syringe? [Re: Pastywhyte]
#18997287 - 10/18/13 08:16 PM (10 years, 4 months ago) |
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You don't need gloves but you should not be handling them much either. You don't want to disturb the dry verm layer so don't touch them anymore than is absolutely necessary. Ambient light on a 12/12 cycle is beneficial at all stages.
almighty thanks!! oh and its WBS not BRF
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Reinoculate forgot to shake syringe? [Re: lonelyjew]
#18997345 - 10/18/13 08:28 PM (10 years, 4 months ago) |
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Quote:
lonelyjew said: almighty thanks!! oh and its WBS not BRF
Nice jumping right into grains Sorry to assume you were doing cakes. Just give em some time. Really you don't want too many spores in there anyway, less is more when it comes to ms
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Eveniftheskyfalls
Even if the sky falls



Registered: 09/08/13
Posts: 449
Last seen: 8 years, 1 month
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Re: Reinoculate forgot to shake syringe? [Re: Pastywhyte]
#18997355 - 10/18/13 08:29 PM (10 years, 4 months ago) |
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Quote:
Pastywhyte said:
Quote:
Eveniftheskyfalls said: I used a syringe that had x4 the amount of spores in it, i also inoculated my jars right after cooking them, which was a very noob move but yeah. I saw little tiny white hairs with a magnifying loop after 2 days
More spores is not necessarily better Many molds have tiny white hairs I'm sure if OP could take a pic through a loop you could solve this for us. Not saying it didn't happen but it sure isn't the norm 
Only the jars growing rhizomorphic had the little white hairs, theyre all growing good around 50% colonization. Sorry if im misleading him im really new to all this
-------------------- Sanity and Happiness are an impossible combination. Even when the sky falls, Ill still be high Anything illegal or has to do with mushrooms that i talk about is completely 100% fictional and is not true. I dont even visit this site. ( I am a proud provider and receiver of positive vibes ) "Reading a trip report is funny. Its like reading a book. You get to visualize the words... Only the visualization is always of some dude starring at the ceiling for a few hours"- Eveniftheskyfalls Progress in life since I found this site (look below)
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lonelyjew
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Re: Reinoculate forgot to shake syringe? [Re: Pastywhyte]
#18997365 - 10/18/13 08:31 PM (10 years, 4 months ago) |
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hey no problem you had no way of knowing!!
that sounds good, im probably just a little over paranoid because its my first grow
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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Eveniftheskyfalls
Even if the sky falls



Registered: 09/08/13
Posts: 449
Last seen: 8 years, 1 month
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Re: Reinoculate forgot to shake syringe? [Re: lonelyjew]
#18997373 - 10/18/13 08:32 PM (10 years, 4 months ago) |
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Sorry haha! and good luck!
-------------------- Sanity and Happiness are an impossible combination. Even when the sky falls, Ill still be high Anything illegal or has to do with mushrooms that i talk about is completely 100% fictional and is not true. I dont even visit this site. ( I am a proud provider and receiver of positive vibes ) "Reading a trip report is funny. Its like reading a book. You get to visualize the words... Only the visualization is always of some dude starring at the ceiling for a few hours"- Eveniftheskyfalls Progress in life since I found this site (look below)
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lonelyjew
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Posts: 300
Last seen: 3 years, 2 months
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Re: Reinoculate forgot to shake syringe? [Re: lonelyjew]
#18997380 - 10/18/13 08:33 PM (10 years, 4 months ago) |
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and could i get optimal times for shaking the grain jars? there is so much conflicting info out there.
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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lonelyjew
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Registered: 05/02/13
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Last seen: 3 years, 2 months
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Quote:
Eveniftheskyfalls said: Sorry haha! and good luck!
thanks!!
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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