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OfflineInsider
Just a random platypus

Registered: 10/18/13
Posts: 56
Loc: Under the sea! Under the ...
Last seen: 9 years, 2 months
Mycelium or contamination?
    #18996812 - 10/18/13 06:29 PM (10 years, 3 months ago)

Hi, Shroomery.
I've been lurking for a while but this is my first post so I will go directly to it:

About a week and a half ago, I made some baby food jars with PDA. Steam sterilized them for 90 minutes and let them to rest overnight just so they would solidify. The next day I inoculated one of them with a P. Cubensis spore syringe and let the jar be for maybe 4 days or so. Two days ago I noticed a growth of sorts inside the jar, but I couldn't see clearly because of the size of it and I couldn't open it because I didn't have a still air box yet (I inoculated it by Lysoling everything around me, cracking the lid just a tiny bit and releasing a drop of spore solution into it).

Today I got a SAB, opened the jar inside of it and found what's in the pics. Also inocculated another jar with the same spore syringe just to see what happens.

I don't know if it is bacterial contamination since I have seen it in agar and doesn't look like the one in that jar, also it took a while to grow. Another jar of the same batch got contaminated with some yellowish stuff and doesn't look like it either.

So what does it look like to you? Myc:thumbup: or trash:thumbdown:?

Thanks. :mushroom2:

inb4: Yes, I know, my sterile techique sucked (sucks).




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OfflineMrcloudy
Stranger than you.
Male User Gallery


Registered: 10/01/13
Posts: 2,889
Loc: Northeast US Flag
Last seen: 3 months, 19 days
Re: Mycelium or contamination? [Re: Insider]
    #18996976 - 10/18/13 07:13 PM (10 years, 3 months ago)

Looks like possibly bacteria.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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Offlinesilverstem
Caps & Stems
Male


Registered: 10/12/13
Posts: 900
Loc: jordan Flag
Last seen: 6 years, 5 months
Re: Mycelium or contamination? [Re: Mrcloudy]
    #18997263 - 10/18/13 08:12 PM (10 years, 3 months ago)

doesnt look like myc


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Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????


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OfflineInsider
Just a random platypus


Registered: 10/18/13
Posts: 56
Loc: Under the sea! Under the ...
Last seen: 9 years, 2 months
Re: Mycelium or contamination? [Re: silverstem]
    #18997910 - 10/18/13 11:00 PM (10 years, 3 months ago)

So should I just toss it away or wait to see if spores germinate?


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Offlinesilverstem
Caps & Stems
Male


Registered: 10/12/13
Posts: 900
Loc: jordan Flag
Last seen: 6 years, 5 months
Re: Mycelium or contamination? [Re: Insider]
    #18998144 - 10/19/13 12:36 AM (10 years, 3 months ago)

toss it and buy petri dishes... retry and do it the way the tek says to do it....


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Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????


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