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OfflineSchmendrick
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Anyone ever done this with Agar wedge?
    #18995461 - 10/18/13 12:44 PM (10 years, 4 months ago)

I am NOT saying it is the best way to do it... but it has worked really well for me, and I am just wondering if anyone else has even tried it...

I have had really fast colonization times (~5 days to 100% colonization) in 1-quart WBS jars by taking the plunger out of a syringe, shoving a colonized Agar wedge into it, shooting the Agar into the jar (it comes out like silly string), then shaking the jar.

Obviously all of this is done in a clean environment with gloves, etc. etc.  My quart jars have silicone-filled injection ports in the lids, and my needles are not small.  I flush the syringe out with alcohol between injections.

The only species I have done it with is Panaeolus Cyanescens.


Edited by Schmendrick (10/18/13 01:26 PM)


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OfflineMush4Brains
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Registered: 07/31/13
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Re: Anyone ever done this with Agar wedge? [Re: Schmendrick]
    #18995491 - 10/18/13 12:51 PM (10 years, 4 months ago)

It seems unnecessary and adds contamination vectors in my opinion :shrug:, but if it works for you, go for it.


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OfflineSchmendrick
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Re: Anyone ever done this with Agar wedge? [Re: Mush4Brains]
    #18995500 - 10/18/13 12:53 PM (10 years, 4 months ago)

Quote:

Mush4Brains said:
It seems unnecessary and adds contamination vectors in my opinion :shrug:, but if it works for you, go for it.




Agreed re: contamination - at least it is probably no better than the regular way of doing it, which is why I am not actually recommending it...  Just curious if anyone else has ever had the idea.

The whole reason I did it in the first place was because I hate opening jars even for an instant to put the wedge in... I sort of feel like I can control the sterility of the syringe better than I can the open mouth of the jar.


Edited by Schmendrick (10/18/13 01:04 PM)


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OfflinePussyFart
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Re: Anyone ever done this with Agar wedge? [Re: Schmendrick]
    #18995599 - 10/18/13 01:17 PM (10 years, 4 months ago)

Quote:

Schmendrick said:
at least it is probably no better than the regular way of doing it



No, it's far worse.

Quote:

Schmendrick said:
I flush the syringe out with alcohol between injections.




Alcohol does not sterilize, so the potential for cross contamination just skyrocket.

We sterilize our tools with heat between transfers, not sanitize them.

And this all just seems like a big waste of time anyways.

Just cut a wedge and drop it in a jar....it's so rediculously simple that people go out of their way to make it complicated.


Edited by PussyFart (10/18/13 01:18 PM)


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OfflineMush4Brains
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Re: Anyone ever done this with Agar wedge? [Re: PussyFart]
    #18995624 - 10/18/13 01:21 PM (10 years, 4 months ago)

OP, you really shouldn't delete your posts.  It's good discussion, even if it was a silly thought.


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OfflineSchmendrick
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Re: Anyone ever done this with Agar wedge? [Re: Mush4Brains]
    #18995634 - 10/18/13 01:23 PM (10 years, 4 months ago)

Quote:

Mush4Brains said:
OP, you really shouldn't delete your posts.  It's good discussion, even if it was a silly thought.




I absolutely hate the thought that some newbie might come in here just skimming through the forums, see this idea and latch onto it without reading the rest of the discussion.  There are too many bad ideas floating around here already.  I don't intend to add to their number.


Edited by Schmendrick (10/18/13 01:25 PM)


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OfflinePussyFart
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Re: Anyone ever done this with Agar wedge? [Re: Schmendrick]
    #18995644 - 10/18/13 01:25 PM (10 years, 4 months ago)

ORIGINAL POST

Quote:

Schmendrick said:
I am NOT saying it is the best way to do it... but it has worked really well for me, and I am just wondering if anyone else has even tried it...

I have had really fast colonization times (~5 days to 100% colonization) in 1-quart WBS jars by taking the plunger out of a syringe, shoving a colonized Agar wedge into it, shooting the Agar into the jar (it comes out like silly string), then shaking the jar.

Obviously all of this is done in a clean environment with gloves, etc. etc.  My quart jars have silicone-filled injection ports in the lids, and my needles are not small.  I flush the syringe out with alcohol between injections.

The only species I have done it with is Panaeolus Cyanescens.





At least my back button still works....

:rules:      2.  Do not ask a question, and then when you get your answer, delete your original post as if we're your personal unpaid research assistants.  For every poster, there's 1000 lurkers who use the search engine to find their answers and never post.  Don't screw them by deleting your question, thus making the search engine return crap.  Users who violate this rule will be banned for at least 3 days.


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OfflineSchmendrick
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Re: Anyone ever done this with Agar wedge? [Re: PussyFart]
    #18995653 - 10/18/13 01:27 PM (10 years, 4 months ago)

Ok fine.  I UN-deleted it.  If you want to take the chance of someone grabbing onto a bad idea and screwing up a grow, that's on you.


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OfflineMush4Brains
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Re: Anyone ever done this with Agar wedge? [Re: Schmendrick]
    #18995666 - 10/18/13 01:29 PM (10 years, 4 months ago)

If somebody uses an untested, unproven idea that was said to be unnecessary and open to contamination issues in the first reply, that's on them :thumbup:.


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OfflineSchmendrick
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Re: Anyone ever done this with Agar wedge? [Re: Mush4Brains]
    #18995697 - 10/18/13 01:35 PM (10 years, 4 months ago)

Quote:

Mush4Brains said:
If somebody uses an untested, unproven idea that was said to be unnecessary and open to contamination issues in the first reply, that's on them :thumbup:.




I'm sure it happens all the time.  There are a thousand 'teks' out there, and 992 of them are ill conceived.  It takes some serious reading to even start to sort the nonsense from the proven methods.  Why should I add more nonsense to the pot?


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OfflinePussyFart
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Re: Anyone ever done this with Agar wedge? [Re: Schmendrick]
    #18995730 - 10/18/13 01:42 PM (10 years, 4 months ago)

Quote:

Schmendrick said:
I'm sure it happens all the time.  There are a thousand 'teks' out there, and 992 of them are ill conceived.



This is hardly a tek.

ANd it has been shot down, for good reason.

If they read past your first post, they won't even attempt it.


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OfflineSchmendrick
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Re: Anyone ever done this with Agar wedge? [Re: PussyFart]
    #18995767 - 10/18/13 01:51 PM (10 years, 4 months ago)

Quote:

Notahacker420 said:
Quote:

Schmendrick said:
I'm sure it happens all the time.  There are a thousand 'teks' out there, and 992 of them are ill conceived.



This is hardly a tek.

ANd it has been shot down, for good reason.

If they read past your first post, they won't even attempt it.




You know, the way you phrase things is really important in communication... there are many better ways you could have said that. You really came off as a jerk.

Maybe it's what you're going for, but it just makes me want to close down this account and go back to lurking instead of trying to be an active member of the community.


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OfflineMush4Brains
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Re: Anyone ever done this with Agar wedge? [Re: Schmendrick]
    #18995774 - 10/18/13 01:52 PM (10 years, 4 months ago)

Being real has the side effect of sometimes seeming like a jerk.  It happens to me 24/7.


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OfflineSchmendrick
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Re: Anyone ever done this with Agar wedge? [Re: Mush4Brains]
    #18995804 - 10/18/13 02:02 PM (10 years, 4 months ago)

Quote:

Mush4Brains said:
Being real has the side effect of sometimes seeming like a jerk.  It happens to me 24/7.




I know, I have the same problem when people come to me with what I consider to be stupid questions and ideas regarding MY area of expertise (IT).  This whole mushroom thing is obviously NOT my area of expertise at the moment, and I understand that it's tough to answer the same stupid questions over and over again, and to listen to asinine ideas day in and day out from newbie know-it-alls... but if you can't do it with at least a little tact, then maybe you shouldn't be doing it at all.


Edited by Schmendrick (10/18/13 02:02 PM)


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Invisibledusttodust


Registered: 11/26/12
Posts: 491
Re: Anyone ever done this with Agar wedge? [Re: Schmendrick]
    #18995815 - 10/18/13 02:04 PM (10 years, 4 months ago)

Quote:

Schmendrick said:
Quote:

Notahacker420 said:
Quote:

Schmendrick said:
I'm sure it happens all the time.  There are a thousand 'teks' out there, and 992 of them are ill conceived.



This is hardly a tek.

ANd it has been shot down, for good reason.

If they read past your first post, they won't even attempt it.




You know, the way you phrase things is really important in communication... there are many better ways you could have said that. You really came off as a jerk.

Maybe it's what you're going for, but it just makes me want to close down this account and go back to lurking instead of trying to be an active member of the community.



being short and concise is good and apropriate, why share unneeded informations? I actually like Notahackers aproach when helping others. Mind that he said nothing offensive and if you feel offended its due to your misinterpretation.

and by the way he actually took his time to answer your question and you are angry because he didnt go flattering you while correcting your mistakes.


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OfflineSchmendrick
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Re: Anyone ever done this with Agar wedge? [Re: dusttodust]
    #18995822 - 10/18/13 02:06 PM (10 years, 4 months ago)

See previous comment.  I don't require flattery.  I require not being downright rude about it.


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InvisiblePastywhyteMDiscord
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Re: Anyone ever done this with Agar wedge? [Re: Schmendrick]
    #18995881 - 10/18/13 02:20 PM (10 years, 4 months ago)

Better get a thick skin if yer gonna hang out in cultivation :lol: Hacker may teach with a hammer but he isn't the only one that does. Pretty much the one rule of thumb here is that you better be able to back up anything you say with pics, science, links, and logic or you gonna be butthurt all day. Even a friendly disagreement here can look like a war sometimes. But if ya can man up a little bit, you can learn a lot :awesomenod:

In regard to your OP and your fear of contams from opening the lid to drop in a wedge, understand that most contams travel on air currents. In a properly constructed and prepared SAB this risk is quite minimal. Under these conditions the next most likely vector for contams IMO is contact. In this case even the contact of the needle to SHIP has a greater risk of carrying a contam into your jar than dropping in a wedge. I find that inoculation with a wedge is one of the most clean and reliable methods there is. Personally I dislike anything in a syringe myself, and the few times that I feel the need to use one to inoculate, I don't even use a SHIP, I just crack the lid and squirt the solution through that, same as if I were inoculating with a wedge.


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OfflineSchmendrick
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Re: Anyone ever done this with Agar wedge? [Re: Pastywhyte]
    #18995932 - 10/18/13 02:35 PM (10 years, 4 months ago)

Quote:

Pastywhyte said:
In regard to your OP and your fear of contams from opening the lid to drop in a wedge, understand that most contams travel on air currents. In a properly constructed and prepared SAB this risk is quite minimal. Under these conditions the next most likely vector for contams IMO is contact. In this case even the contact of the needle to SHIP has a greater risk of carrying a contam into your jar than dropping in a wedge. I find that inoculation with a wedge is one of the most clean and reliable methods there is. Personally I dislike anything in a syringe myself, and the few times that I feel the need to use one to inoculate, I don't even use a SHIP, I just crack the lid and squirt the solution through that, same as if I were inoculating with a wedge.




^^ That's a good response.  It explains why it's a bad idea all around without being rude.  Shooting people down, concisely or not, just makes it less likely that person will post in the future.

Thank you for your response, Pastywhyte.


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InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: Anyone ever done this with Agar wedge? [Re: Schmendrick]
    #18995939 - 10/18/13 02:37 PM (10 years, 4 months ago)

The idea has its flaws, sure, but I like it. :smile:


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OfflinebEelzeBosS
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Registered: 06/15/13
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Re: Anyone ever done this with Agar wedge? [Re: Pestile]
    #18996093 - 10/18/13 03:16 PM (10 years, 4 months ago)

I would pressure cook the syringe before I tried it (assuming it wouldn't melt, no idea if syringes could withstand 15 psi) and apply the wedge to the plunger in a SAB.

This could actually help me, Ive been having a weird problem - when I add an agar wedge to a grain jar then shake it up, half of the agar wedge gets stuck to the lid of the jar and no matter how hard I shake it just stays there with WBS stuck to it. I then have to let the jar colonize upside down. Its happened to me 4 of my last 10 tries. This method would certainly eliminate that problem.


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