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OfflineJMcDoogle
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Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 3 years, 9 months
Need Help Sailing through Endless Skies - Starting Up Again
    #18993750 - 10/18/13 12:14 AM (10 years, 3 months ago)

Hello, and welcome to my misguided, informed, and quite lazy forum post! I am JMac! I would like to thank you for taking the time out of your time to glance at my grumblings. With that said, I have had a sudden realization that I do not have a productive hobby, so what else is better than cultivating. I will begin with a slight introduction.


I had previously grown shoddy and inpatient in my younger years ( not that long ago really ) using a PF-TEK with BRF+VERM and had a few success stories, I will have more questions than admissions in the beginning chapters of this post. So here is what I have as of now;

5 10-CC Spore Syringes from LSS - 2EQ,2GT,1MALABAR
Plenty of Jars
125 100x20 Three-Section Plastic Petri Dishes
1Bottle Agar Powder
23 QT PC
100's of Single Use Sterile Packaged Syringes
4-Blunt Applicator Needles
Brown Rice
Verm
Myco - Coir

I am doing heavy reading latley and I find to admit that I have endured myself into a daunting task of the begging stages of a very beneficial hobby, though rough at first - with work it will become fruitful, yes?

My one thing is, I dont like spending money frivelously, I have ordered the five strains(B+ and A-Strain were unavaliable,shame,) and I would like to know what my friendly viewers professional opinion is on how to best optimize the use of my five 10-cc liquid syringes into long lasting supply of culture. Possible Bulk prep after creating 1QT (or1pnt) spawn jars, probably using BRF+VERM tech at first, maybe WBS...

Giving what I have, how would you approach this endevour in the initial months - my plans are to; using what I have now create a sustainable source of cubes using a monotub bulk system with coir,verm,glycose.

I know I am vague, and probably stupid, please forgive these and offer your opinion without descretion.

------
10/27 Update
------

Quote:

Will update OP -

7 of 8 PP5 Containers show noticable growth - inoculated in glovebox using syringe on 10/25 around 5pm

The LC jars inoculated on 10/24, hard to tell, I think I did Tablespoons of Karo instead of TSP so the liquid is very dark, a flashlight does not help either. We will see in a week or so whether or not they will be viable.

inoculated ten quarts ball jars on 10/26 early midnight and 10/26 mid-day

We will see how they look here in a few days.

The pp5 RGS is 2 - 1/2 pints of each variety I have ordered, and one will be used for g2g, and another will be fruited for spore prints to go onto agar.




------
10/30 Update
------


------
11/3
------

Some pictures - will add information later.


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11/8
------
Here is a photo update! If you look from the last photos to these, you ccan see I've added a few more jars :wink:



The two pictures of the single jar, I am concerned about -

It looks really heavy, albeit it may be caused by the grain shrinkage and it trying to reach out to the glass - You be the judge of that - I was going to do another ten or so jars tomorrow using that a master g2g -

Its hasnt been that long, but it looks like my original idea of having a lasting supply from a few cc's is on its way to becoming a reality - thanks to all who have helped and inspired me along the way!

Onward!

------
11/18
------


I've done my first 56 Qt Mono -

I've got another 3 16 qts mini-monos and 4 6qt Mini-Mini-Mono's
Going aswell. Update on Jars I have 36-40 Quarts of Spawn Going
Currently, 10 or so plates of Agar - Including some Wild Aussie
spores generously sent from another member!

Onward!

---

“The meaning of life is just to be alive. It is so plain and so obvious and so simple. And yet, everybody rushes around in a great panic as if it were necessary to achieve something beyond themselves.”
― Alan Wilson Watts, The Culture of Counter-Culture: Edited Transcripts


Edited by JMcDoogle (11/17/13 08:47 AM)


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Offlinemushrume man
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #18993846 - 10/18/13 01:10 AM (10 years, 3 months ago)

Spores to agar, isolate a sector, agar wedge of (semi) isolate to wbs qts, g2bulk, clone a pin or fruit on agar, save master slant in fridge. Repeat for each strain.

This is best way to use spores to start, may be missing some things, the rest is up to you. Always nice to start with so many supplies! Good luck

Edit: You could make an Lc or GLC for brf cakes in place of wbs/bulk steps


--------------------


Edited by mushrume man (10/18/13 01:16 AM)


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InvisibleGeorge Sears
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: mushrume man]
    #18993862 - 10/18/13 01:18 AM (10 years, 3 months ago)

Congrats on your starting up again with this hobby! Boy, do I envy you right now..

Anyway, if I were you I would skip the crap with BRF cakes. That, however, depends on how much you'd like to grow. I personally recommend using WBS or RGS and using that to spawn to a bulk substrate. The reason for this is that, often, with cakes you're constantly worrying about maintaining the proper environment for them by manually misting about and fanning at least a few times a day; however, with a bulk monotub the growing process is basically set-and-forget if you take the proper sterility precautions.
Yields are far greater and I personally feel like you get more of your money's worth this way.

Just my opinion though. :shrug:

Edit: After actually reading your post carefully I realize that you already planned to spawn to bulk.. :smbfacepalm:

Still though, if you just sterilize an assload of WBS or rye berry/GS jars then you can just use pre-colonized jars to inoculate other jars without actually having to use more spore solution from your syringes.

This, hypothetically speaking, means you would only need about 1-6cc of whichever variety you'd like to grow in order to colonize about 40+ jars when it's all said and done.


--------------------


Edited by George Sears (10/18/13 01:23 AM)


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OfflineJMcDoogle
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Registered: 07/07/09
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: George Sears]
    #18998568 - 10/19/13 05:45 AM (10 years, 3 months ago)

Thanks for the replys. Your input will not go unnoticed. Heres what I think i'll be doing once everything arrives.

Today, I'll be making my way to the store to purchase 50# or so of Rye Grass Seed (from now on i'll be using RGS to shorten it up.) My spores will be arriving today, though my new 23qt pressure cooker probably not until tuesday. I may begin tomorrow to saturate the grains and allow them time to dry properly, perhaps even indulge in the plant food avenue... perhaps. So I'll have my syringes, but my agar petri dishes will not be here until tuesday most likely. I have hundreds of 10cc individual wrapped syringes, i'll ask a question of those later. So without the Agar prepped and ready as of now I believe I will be using the spore syringes as early as tuesday night / wednesday morning once the pc has arrived and I can properly sterilize the grains.

I believe strain questions should be directed to a single thread, but as this is my topic I believe I am okay in asking which one do you believe I should start off with.

I have yet to create a GH - though I do have 2 extra walk-in closets with little to no airflow measuring at 8x4 - I believe this space will be sufficient.

I find it hard to believe sterilite and other clear containers can range up to 30-40-50$ a piece, wow, looking forward to finding some at a discount somewhere.

So, to sum up the questions...

If possible, could anyone recommend the most cost-effective GloveBox - Zero Air Box construction.

What are the expected limitations in such a room 8x4 with 10 foot ceilings. ( I do have an extra room 13x12 with closet, though very dusty and has furnace inlet/outlet. )

Should I wait for all of the agar materials to arrive, thus creating glovebox to create agar petri dishes using Spore Syringes from LSS, or perhaps create a spawn jar by using 1-4cc of syringe in a traditional PF-TEK style jar using RGS or BRW/VERM - than use living culture from the jar for g2g transfers, hope everything goes right and create spore prints / pin or flesh agar transfers, perhaps maybe liquid culture?

I will be trying the use of Ziplok twist-n-seal and using Violets tek for my first attempt at my newfound hobby. Anything else I should think of -

Again thanks for reading my grumblings if you have, I know I am not the most informed/organized, but if have waded through this post and came out unharmed, please do offer any thoughts/inputs you may have without discretion.

EDIT: Oh, I forgot to ask, with the individually wrapped sterile 10cc syringes, I have four long blunt applicator needles that will fit perfectly, if I were to make a LC of some sort, would I be able to take of the applicator needle, and leave uncapped of any sort, and perhaps wrap with foil, I do not have any covers for them of any sort - just the four long steel applicator needles. Thanks!

“Things are as they are. Looking out into the universe at night, we make no comparisons between right and wrong stars, nor between well and badly arranged constellations.”


--------------------
The ego is nothing other than the focus of conscious attention.


Edited by JMcDoogle (10/19/13 05:47 AM)


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OfflineStromriderM
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #18998576 - 10/19/13 05:50 AM (10 years, 3 months ago)

Another poor victim :facepalm:

I have a start to finish bulk tek in my journal. These methods I am advocating in my journal are tried and true. Check it out if you really wanna grow some mushrooms


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OfflineJMcDoogle
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Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 3 years, 9 months
Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #18998617 - 10/19/13 06:26 AM (10 years, 3 months ago)

Quote:

Stromrider said:
Another poor victim :facepalm:

I have a start to finish bulk tek in my journal. These methods I am advocating in my journal are tried and true. Check it out if you really wanna grow some mushrooms




Could you possible reiterate for clarity what you mean by saying another poor victim - thanks in advance if you do.


--------------------
The ego is nothing other than the focus of conscious attention.


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OfflineStromriderM
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #18998624 - 10/19/13 06:34 AM (10 years, 3 months ago)

I was referring to you wanting to grow with the Violet tek but I really don't wanna start up this debate again. I just hate  to see people put all that time and effort in just to end up with a bunch of poor spindly looking shrooms. Your time and effort would be much better spent using time tested methods that yeild undeniably great results.


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OfflineJMcDoogle
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Registered: 07/07/09
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #18998989 - 10/19/13 09:50 AM (10 years, 3 months ago)

Stromrider, Thanks for the input!

I was at a local store just not and I was like hell, wonder what the ridicilous price on ziplock containers is here, and I dont know if I made a bad buy but I got Six Three Packs at 2.99 a piece - they were on sale.

So, basically a dollar a piece, I bought all they had so I decided to do a rain check for ten more units. I stopped by the feed store and they had some really good Perinial Rye Grass Seed for around 1.95# which I think is pretty pricey, so I just grabbed ten pounds, should be enough to start at the very least.

PS; as I was writing this post my cat was on my lap purring, started licking my cheek affectionatley and than decided to bite me out of nowhere.... really hard, I am bleeding. Cheers -


--------------------
The ego is nothing other than the focus of conscious attention.


Edited by JMcDoogle (10/19/13 09:51 AM)


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OfflineStromriderM
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #18999008 - 10/19/13 09:55 AM (10 years, 3 months ago)

Damn I think I would smack the shit out of that pussy! :lol:


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InvisibleViolet
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #18999296 - 10/19/13 11:24 AM (10 years, 3 months ago)

Quote:

Stromrider said:
Another poor victim :facepalm:



Quote:

Stromrider said:
I was referring to you wanting to grow with the Violet tek but I really don't wanna start up this debate again. I just hate to see people put all that time and effort in just to end up with a bunch of poor spindly looking shrooms.



Look new fella, if you "really don't wanna start up this debate again" then why would you drop those kinds of false statements?
I see you dropping untrue and half-true assumptions all-over these forums lately, often following or stirring such childish dramas.


In my experience it's pretty safe to say that if people don't get good results they either don't know proper conditions or the water element, or they don't select genetics; that or they simply did poorly somehow else, a newbie is a newbie.

Mushrooms don't grow "spindly" because of a tek. Try convincing anyone that straight-grain necessarily results in "spindly" mushrooms.
If you really believe it does you are simply ignorant to the obvious facts.

Some first flushes... (multi-spore even)


3rd flushes from some mediocre clones...


5th flushes even!


Quote:

Stromrider said:
Your time and effort would be much better spent using time tested methods that yeild undeniably great results.



Of course using bulk substrates is an entirely viable way to grow, especially to people who don't yet fully understand providing proper moisture and humidity conditions to their grows, and for those that prefer lean on the roundabouts of a tek instead of an implemented in-depth understanding of the multiple grow facets.
With both, even, results can be incredibly impressive and one could only be exited about pulling off results like this.
I can't help but wonder how growers like him, who truly get conditions for their grow method, could do if they gave a shot to adjusting their knowledge to a method like mine. Certainly he would do even better than me.

I had quite bumper output with bulk many times myself:

But as I had them and watered cased grains next to each other in the same chamber I began to see their differences. I definitely had to tweak my conditions and process, but once I learned the simple subtleties of ideal conditions (which bulk did not really help me learn but instead works around) I realized the watered cased grains were giving me a better yield over time and cut many big steps out of my process.  Not to mention they dried more quickly and people liked them more...  I can't imagine an experienced grower not catching that happen right in front of him of her.  Big parts of my process went entirely unused; the biggest sub, the biggest water, the biggest energy, the biggest clean work, the biggest failure risk juncture, the biggest garbage. Yet my big output stayed consistent and my friends start telling me they can plainly tell a difference too.
As they say, ":shrug:"


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


Edited by Violet (10/19/13 11:30 AM)


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InvisiblePestile

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Violet]
    #18999338 - 10/19/13 11:33 AM (10 years, 3 months ago)

Nice! :thumbup:
Those albinos look beautiful.


--------------------


The Corbett Report
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InvisibleViolet
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Pestile]
    #18999393 - 10/19/13 11:46 AM (10 years, 3 months ago)

Thanks!  They're the same exact culture in fact


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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OfflineStromriderM
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Pestile]
    #18999400 - 10/19/13 11:48 AM (10 years, 3 months ago)

But honestly Violet could you agree with the statement that an inexperienced grower using ms has a better chance of getting good results spawning grains to a bulk substrate

That is what my argument. I am not trying to pick a fight with you. I should have went with my instinct and kept my mouth shut. I knew this would happen :facepalm:


Edited by Stromrider (10/19/13 11:49 AM)


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InvisibleViolet
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #18999441 - 10/19/13 12:00 PM (10 years, 3 months ago)

No I cannot agree with that statement.  Bulk techs involve learning not only the simple sterile work my tech uses but also additional materials, treatments, and procedures that increase failure factors.  On a gradient of incremental learning and ease of successful completion I'd order it truffles, PFtek, agar, 'violet tek', bulk sub teks starting with edible isolates then multi-spore cubie, exotic species.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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OfflineJMcDoogle
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Violet]
    #18999450 - 10/19/13 12:01 PM (10 years, 3 months ago)

Quote:

Violet said:
Thanks!  They're the same exact culture in fact




Blessed are the hearts that can bend; they shall never be broken. To each his own - Violet, first and foremest, wow.. you're posting in my thread, welcome!

I have decided to go with VTEK first, alongside a RR' style rye berry bulk grow - I am going to be following each procedure to scientific standards, hopefully.

As I said in the past, I have 5 10cc LiqSporSyr, 2 EQ , 2 GT , 1 Malabar - Will be Getting Prints/Syringes of A-Strain,B+,and Thai in around two weeks.

I think the right thing to do in this situation is take around 1-2cc of each syringe and inoculate sterile RGS containers for each strain - as I await the arrival of my peteri dishes and purchase everything needed for correct agar.

I will be Isolating sectors of Rhizo growth ( I think thats the correct termonolgy ) over a series of two weeks maybe for each strain - working towards a fuller and singular isotype/isotope/isolate growth or whatever you may want to call it of each psy.cub/genus/species that I have. In the mean time also having some fully colonized grain will be good for some g2g or g2lc... ahh, I've engulfed a magnitude of information in the last two days, coupled with stress and running around I think I may be compiling jibberish that is nonsense and quite appauling. Regardless, these are my ideas - again please leave feedback without discretion.

I hope I did not scare away Violet...*waves.*


--------------------
The ego is nothing other than the focus of conscious attention.


Edited by JMcDoogle (10/19/13 12:03 PM)


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OfflineStromriderM
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Violet]
    #18999455 - 10/19/13 12:03 PM (10 years, 3 months ago)

:thumbup:
I disagree but we are both entitled to our own opinion


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #18999487 - 10/19/13 12:11 PM (10 years, 3 months ago)

Hahahah, VTEK is funny, like a Honda engine.

$2.99 for 3 containers isn't bad, the best I find 3 for is $2.59
FYI, those containers also work great for pre-poured agar as an alternative to single-use petris...

Since you have syringes I'd suggest you just put them to grains. Spore solution often doesn't fare well on petris particularly for peeps new to sterile technique.
You can make a very thin cake of grains in those containers – I call it "grain petri" & will be posting about it soon – and put just 2 or 3 droplets in the middle just as you would a petri. The spores stay and germinate together, no rolling around in water on agar, but grow outwards just like on agar.
Also, tweezers! My favourite.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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OfflineJMcDoogle
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Registered: 07/07/09
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #18999489 - 10/19/13 12:11 PM (10 years, 3 months ago)

Reasons why vTEK stands out above all others I've muddled around with in my brain. Again, I am not experienced, I've only taken in information at this point.

1 Grain
Plastics ( can I get a hoorah? )
Less Change of Contam Spread / Lost Materials In Mishaps
Space Saving
Option to spawn into bulk if desired.
Clear Directions
Valid Arguments..

I cannot be close-minded to something that has shown great results with little failure rate following proper sterile tech and procedure - It seems easy enough and has got my bid for the most appealing to me atm. Its not to say that I wont be doing a RR Style Rye alongside, but I see no major faults in either tech.


--------------------
The ego is nothing other than the focus of conscious attention.


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OfflineJMcDoogle
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19006813 - 10/21/13 01:00 AM (10 years, 3 months ago)

I posted this as a thread in the advanced section and in two days nobody has responded, maybe I'll get some help here -

Quote:

http://www.fpl.fs.fed.us/documnts/pdf1994/burds94a.pdf




This is the reference article -

Preserving Cultures Using Sterile Distiled Water in Cryovials

Abstract:

Prior to 1985, cultures at teh center for forest mycology research were maintained on a 1.5% malt extract agar test-tube slants. This system not only made it neccessary to transfer the entire collection every year but also permitted genetic change because continual growth occured. In 1985, the method of storing fungal cultures in sterile distilled water in CRYOVIALS was introduced. This study reports on the use of this method for long-term fungal storage. For varying periods of up to seven ( 7 ) years, 151 miscellaneous specials of wood-decaying Basidimycotina were stored in sterile distilled water. Water storage has numerous advantages: culture viability or growth rate is not significantly influenced; isolates can be stored longer; genetic stability is greater; the method is quick, easy, and inexpensive, and requires less space.

Cyrovials ( Internally Threaded & Self Standing

These can withstand autoclaving so if I were to buy them and try something along the lines of distilled water long term storage, I could always uses them as slighty more expensive alternative to the glass vacutainer blood collection tubes.

Heres what Im guessing - seven years, of unrefigerated storage is a possiblity, but even two years unrefigerated would be nice aswell.

Full Sterile - with option of threaded inside, or external -
which would you prefer - internal or external threads?

Take a fully colonized grain, or spore print - isolate, isolate, gather your monoculture - inside of SAB/GB/FLOW/FKN LABRATORY

Take 4-6-8 ML of Sterile Distilled Water and put onto your agar monoculture

Scrape it a bit, mix up the myc.

Suck it back up and put into the Cyrovial -

Than go about your business and save for 5 years and when needed re-enter onto agar - break down any contams if any - and sustain your strain? Or take fully colonized agar chunk, cut into 2x2 pieces and drop three or four into the cyrovial filled with sterile distilled water, seal and store. They feature a rubber type gasket internally and can be twisted completley air-tight aswell, so no need to wrap them.

Im new to all of this, so this all might be a huge waste of brainpower. Please leave any and all feedback, discreation unneccesary, so... what you think?  :sorry:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19007103 - 10/21/13 04:28 AM (10 years, 3 months ago)

Quote:

JMcDoogle said:
Thanks for the replys. Your input will not go unnoticed. Heres what I think i'll be doing once everything arrives.

Today, I'll be making my way to the store to purchase 50# or so of Rye Grass Seed (from now on i'll be using RGS to shorten it up.) My spores will be arriving today, though my new 23qt pressure cooker probably not until tuesday. I may begin tomorrow to saturate the grains and allow them time to dry properly, perhaps even indulge in the plant food avenue... perhaps. So I'll have my syringes, but my agar petri dishes will not be here until tuesday most likely. I have hundreds of 10cc individual wrapped syringes, i'll ask a question of those later. So without the Agar prepped and ready as of now I believe I will be using the spore syringes as early as tuesday night / wednesday morning once the pc has arrived and I can properly sterilize the grains.

I believe strain questions should be directed to a single thread, but as this is my topic I believe I am okay in asking which one do you believe I should start off with.

I have yet to create a GH - though I do have 2 extra walk-in closets with little to no airflow measuring at 8x4 - I believe this space will be sufficient.

I find it hard to believe sterilite and other clear containers can range up to 30-40-50$ a piece, wow, looking forward to finding some at a discount somewhere.

So, to sum up the questions...

If possible, could anyone recommend the most cost-effective GloveBox - Zero Air Box construction.

What are the expected limitations in such a room 8x4 with 10 foot ceilings. ( I do have an extra room 13x12 with closet, though very dusty and has furnace inlet/outlet. )

Should I wait for all of the agar materials to arrive, thus creating glovebox to create agar petri dishes using Spore Syringes from LSS, or perhaps create a spawn jar by using 1-4cc of syringe in a traditional PF-TEK style jar using RGS or BRW/VERM - than use living culture from the jar for g2g transfers, hope everything goes right and create spore prints / pin or flesh agar transfers, perhaps maybe liquid culture?

I will be trying the use of Ziplok twist-n-seal and using Violets tek for my first attempt at my newfound hobby. Anything else I should think of -

Again thanks for reading my grumblings if you have, I know I am not the most informed/organized, but if have waded through this post and came out unharmed, please do offer any thoughts/inputs you may have without discretion.

EDIT: Oh, I forgot to ask, with the individually wrapped sterile 10cc syringes, I have four long blunt applicator needles that will fit perfectly, if I were to make a LC of some sort, would I be able to take of the applicator needle, and leave uncapped of any sort, and perhaps wrap with foil, I do not have any covers for them of any sort - just the four long steel applicator needles. Thanks!

“Things are as they are. Looking out into the universe at night, we make no comparisons between right and wrong stars, nor between well and badly arranged constellations.”





Dude that is a ton of work for a really poor survival chance. I really wish you the best, but forget all that RGS bull. I tried and tried to make it work, it didn't. It was harder to get the water ratio correct than WBS, and I still have yet to test the strength of the fruits. (for the record I have a half stalled tub of this nightmarish RGS)

  Do your self a favor and make 15 Jars of BRF- 3 of each strain. This means you should still have 7cc's left in each syringe. Now start the Agar process. The 15 Jars can be your backup plan incase you have some trouble with Agar, you will still have a method to produce some mushrooms, and also make your self tons of prints.

  For the record, I am not saying anything against a plastic bottom watering tech, or RGS as a substrate. I have honestly noticed that RGS just does so much better in my back yard. My yard is green and fluffy, and it is so soft when you walk on it. RGS is amazing when applied to soil...were it should be V.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Sockadin]
    #19007115 - 10/21/13 04:44 AM (10 years, 3 months ago)

Thanks Sockadin,

I am going back and fourth in my mind -

But I keep going back to this tried and true, simple and effective tek. I believe I will take on violets for fun later, a month or so perhaps.

I think I am going to just use RR's tek, or a simple BRF, and do the jars like said - saving enough liquid for later use, and having a good amount of culture for grain to grain - bulk, and all things go well, plenty of spore prints. Again

As you said, all the while doing the jars and mini-mono's or simple cakes, working on my agar recipes and isolates, perfecting the craft. Hell, time is going to be spent regardless of agar work, and dishes are cheap as it is, might aswell create a super-"strain" while watching my jars colonize and babies fruit :wink:

-- Any input on the whole Cyrovials?


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Sockadin]
    #19007373 - 10/21/13 07:37 AM (10 years, 3 months ago)

Quote:

Sockadin said:
I really wish you the best, but forget all that RGS bull. I tried and tried to make it work, it didn't. It was harder to get the water ratio correct than WBS, and I still have yet to test the strength of the fruits. (for the record I have a half stalled tub of this nightmarish RGS)

Do your self a favor and make 15 Jars of BRF- 3 of each strain.

  For the record, I am not saying anything against a plastic bottom watering tech, or RGS as a substrate. I have honestly noticed that RGS just does so much better in my back yard.  RGS is amazing when applied to soil...were it should be V.



So you failed to prep RGS well. That doesn't make it "all that RGS bull", you should know better.

How sensible would it be to say Rye is for bread where it should be, WBS is for the birds where it should be, or brown rice is for dinner where it should be?


If you boil RGS a lot, it has to be strained a very long time due to all the excess water held amongst the many tiny grains.
However it's possible to adjust your preparation based on that very problem, like the grain prep for my tek. By only briefly boiling the grains to get them started you make it so that the water stuck amongst the grains is just right to finish hydration. Toss them in a strainer until most of the dripping stops and load up for sterilization.
I've done it many many times and always end up with a perfectly hydrated, loose, fine grain substrate that colonizes extra quickly with thick rhizos and gives me the best results of any grain I've yet used.



The cryovials work, although it may be a bit much for sterile work for someone new to sterile tech.
The abstract about it mentions transferring test-tube slants every year, which I'm pretty sure isn't nearly as necessary as the abstract says. We often keep slants for a decade, perhaps even more.
Culture vials may or may not be a better option for keeping cultures preserved but regardless it's still a bit of a risk, as you probably will not find out until the far-off day you decide to use it whether it's sterile for use or not.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Violet]
    #19009384 - 10/21/13 04:46 PM (10 years, 3 months ago)

Let me first address Violet. Then the OP.

Violet, we all think your grows are amazing, I am not saying that. I think you have some amazing results and definitely know what your talking about. My comment about RGS Bull, is only in relation to the struggle that I had with it. It works in the plastic tech, but it is a bitch to use as a true grain. The seeds colonize so tightly that they are a pain to remove from the jars, and you get a lot of clumping.

OP, if you follow Violet's tech you may very well have great results. But is it worth the cost of the equipment, prep and setup time?

Violet, I believe you have created a very easy to read and useful series of teks, and most of the information in them is useful beyond helpful. I love reading your theories and opinions on what is happening in specific situations and why. However, I personally would not recommend them to a new grower, or someone who is looking at getting back into growing because the over all cost and time consumption is higher than the standard PF Tek, and if it fails, the grower might have a larger sense of frustration when considering the costs and time.

  I would however recommend your teks to experienced growers, and would love to see what someone like Frank could end up with following your tek.

OP, I think it is awesome that you are coming back into this experience, as I myself have recently decided to continue my journey. It is with helpful people and civilized constructive conversation that we advance the knowledge and understanding in to community.

  Good luck with your grow!

  Violet, as always, it was a pleasure conversing with you and your perspectives.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Sockadin]
    #19009515 - 10/21/13 05:18 PM (10 years, 3 months ago)

Wow sock that was very well said. I'm impressed


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19010326 - 10/21/13 07:48 PM (10 years, 3 months ago)

:thumbup:

Im not trying to start a war, Im just defending what I think would be most productive for the OP.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Sockadin]
    #19010333 - 10/21/13 07:50 PM (10 years, 3 months ago)

I know


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19010580 - 10/21/13 08:36 PM (10 years, 3 months ago)

Thanks Sockadin.


Just one thing though... With the exception of not needing a PC (which even PFtekkers that want to keep growing will surely end up with one) my tech is just as inexpensive as PF (if not less; doesn't require vermiculite or perlite nor the elaborate maiming of a fancy FC).
Just a $2 2lb bag of brown rice is substrate for a dozen containers.

I've done PFtek many times and it certainly takes more time & effort than my current tek. Measuring/mixing, loading, wiping, verm-barrier-ing, steaming, needle-flaming, 4-inoc-per-jar-ing, long waiting, dunking dunking dunking... and for what output?
I boil, strain, sterilize, inoc, wait, bottom-water... and thoroughly enjoy the output.

As for what is a good recommendation for a new grower... Not every new grower is the same. Neither are newbies and "n00bs" really the same thing either; everyone is new at their beginning but certainly not everyone is a "n00b" as it's meant.  I agree that your recommendation is best for a "n00b" but I disagree that such a recommendation is called for to all beginners as if they're all on the same level. Who are we to determine which tek is right to recommend to each person? Is making only a single recommendation the right way to approach that?

My first successful grow was a "bulk" grow, and I was more than ready & able to start with that. I understand that everyone may not be like that, but I certainly had NO related skills, hardly even knew how to keep plants! If I could do it, anyone short of someone noobish certainly can.
I mixed PFtek along with my 2nd set of grow endeavors and learned quickly that it's extra annoying for so little payoff. From the angle I had it was hard to see why PFtek is supposed to be ANY easier.  IMO it's really only because a pressure cooker is not required. If someone has/gets one I don't see why they might as well not go straight for "bulk" grows. Honestly I think the self-maintaining bulk sub and mono FC is easier than PF cakes and a somewhat complex Shotgun FC (consider how many noobs we inform that their SGFC is improper).
There is no single scale of "easy" that applies to all learners.


Not to forget, it's not only the OP that reads our posts here. It's important to remember that what we say to each other may be read by each other once or twice but by complete strangers hundreds of times.


EDIT: I'm reminded to add that I mostly made the above statements about relative newbishness because our OP here is definitely smart and is doing his research. He's already talking about culture storage and sterile work. IMO recommending the likes of PFtek is particularly unfitting for him.  He may be new but he's got on his papa shoes.


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Edited by Violet (10/21/13 08:47 PM)


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Violet]
    #19010643 - 10/21/13 08:52 PM (10 years, 3 months ago)

I definitely agree with that. Doing pf tek and operating a sgfc is a pain in the ass and yeilds poor results for the amount of effort it takes. I don't find growing bulk anymore difficult. You just have to be able to read and follow instructions.  Some people just have a hard time with that I guess


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19011147 - 10/21/13 10:42 PM (10 years, 3 months ago)

Wow guys, I really appreciate your constructive arguments here, I really do. Please stay with me through this process * hugs *

This is the same question as before - I have done research, almost too much. Beyond the Violet Tek, which I will be running simultaneously - How would you go about this.

I want to do a minimal contam rate initial grow - to best use my avaliable 50'ccs of P.cubenis liquid spores.  Someone recommended 3 quart jars of each, making 15 quarts total.

Should I start with Organ Rye Berries, or Wild Bird Seed? Brown Rice Flour? I want to be able to do a grain to grain possibly for bulk innoculations in a clean-room or glovebox/sab. I may be a beginner, but I believe I can follow directions diligentially.

How would I begin to do my Agar, wait for a fully colonoized jar of each " strain " and take a piece of grain and drop onto the dish, or take one of each three quart jars, and create a liquid culture? Or simply take a drop of each syringe and put one drop onto each section of the three tiered petri dishes?


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19011240 - 10/21/13 10:58 PM (10 years, 3 months ago)

I would start with wbs. It is pretty forgiving with getting the moisture content just right. You could just use your syringe to inoculate a couple wbs jars then take your best looking jar and use it to inoculate several more with g2g transfers 

If you want to inoculate agar just use a drop from your syringe.  Or better yet just use your syringes to inoculate grains and/or cakes and save your agar for use with your own spore prints and for doing tissue clones


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19011301 - 10/21/13 11:11 PM (10 years, 3 months ago)



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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19011498 - 10/21/13 11:51 PM (10 years, 3 months ago)

I always do g2g. I have a good friend that always does glc. We are both successful so I know they both work. I guess it's just personal preference


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19011500 - 10/21/13 11:53 PM (10 years, 3 months ago)

Say I wanted to do a bulk g2g - I mean 100's of quarts...

Where would I find the cheapest Jars, from glass to plastic, anything resuable -

Im guessing widemouth Ball Quart Jars? But Arent they like 20$ for 15 of them?

Bags Perhaps?


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19011530 - 10/22/13 12:01 AM (10 years, 3 months ago)

Yeah probably spawn bags. How many mushrooms does one man need? :lol:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19011546 - 10/22/13 12:05 AM (10 years, 3 months ago)

One and done, minimialize risk by taking advantage of the time used to create one large batch, and store clean materials until I need them in the upcomming years, I say this, than I'll probably become addicted to the actual hobby of creation - and do a more legal edible/exotic grow to hone in on my skillsets -


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19011551 - 10/22/13 12:08 AM (10 years, 3 months ago)

Yeah you're already hooked and you don't even know it. Mushroom Cultivation is a very fun and satisfying hobby


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19011741 - 10/22/13 01:01 AM (10 years, 3 months ago)

No, theres no way. Its not possible...



Okay, maybe a little bit -

I have central heating and its really dry in here - going to have to experiment with a hygrometer ( think thats what its called ) and a cool mist humidifier and a steam humidifier so I can atleast have the outside of my GH / FC not so try as to leech the humidity at every chance it gets.


Pressure cooker arrives tomorrow, will be prepping grains in the morning before work, and after work.. well..

:dawerp:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19011755 - 10/22/13 01:07 AM (10 years, 3 months ago)

Awesome!

I run a humidifier in the room where my tubs  are  24/7 It is an ultrasonic humidifier.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19011794 - 10/22/13 01:17 AM (10 years, 3 months ago)

This is the one I think I am going to purchase tomorrow.


I think this will do the job? What do you think?

Thanks! PS - I sense a bromance forming, alteast on my part. Or maybe its just one of those teacher student romances. Creepy, I know right?

:nicesmile:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19011848 - 10/22/13 01:33 AM (10 years, 3 months ago)

That looks like a nice humidifier but I don't really know much about them.

Btw.... Your a homo :lol:
j/k you're alright man. We can have a bromance if that is what you really want. My wife might get jealous though


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19011870 - 10/22/13 01:39 AM (10 years, 3 months ago)

When I do my grow log, which I am reluctant to do with the legality of this hobby, Im actually thinking of doing it in this thread, rather than creating another thread to clog up the pages.

So, when the shops open, buying WBS - I think its best to buy without corn added right? Also I'll be buying Organic Rye Berries, I already have ten pounds of Rye Grass Seed -

Perhaps I'll do 4 Quarts of Each "strain" syringe - 2 WBS, 2 Rye, and 1 RGS in the ziplock containers, and see how they colonize and such.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19011990 - 10/22/13 02:22 AM (10 years, 3 months ago)

Yeah try to find wbs with the least amount of sunflower and corn. The red and white bags at Walmart work really well. They're cheap too. I just hate going to Walmart! That wbs is about the only reason I go to that God forsaken place :lol:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19012009 - 10/22/13 02:31 AM (10 years, 3 months ago)

http://gigabiting.com/wp-content/uploads/2011/04/walmart.jpg

Yes, I agree with you on the walmart issue. I find it really hard to go there, even with stores such as meijers * yes I know saying meijers give away my region, who cares * who are high on a corporate ladder, but still give more back to the united states.

I will on the other hand be paying up to 25% more for the wbs as I did with the Rye Grass Seed, and will be doing with the Rye Berries by choosing a local family run Feed Supply, they have lots of selection, most business is done to local hunters this time of the year anyways so I know the stock on berries and bird seed will be fresh, the prices are higher, but they're people just like me trying to make a place in this world - gatta give em credit!


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19012031 - 10/22/13 02:42 AM (10 years, 3 months ago)

Hell yeah thats what I like to hear. I tried buying wbs from my local feed store but it was so full of cracked corn that I didn't like using it. Plus the owner of  my llocal feed store is a friend of the family so I would have to answer a bunch of silly questions like hey man why do you buy so much bird seed or man you are going to have some fat birds!

You know how that goes


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19022850 - 10/23/13 11:42 PM (10 years, 3 months ago)

I just wanted to update you all -

I've begun  :feelsjohngoodman:

WBS - enough for twelve 1 quart
RGS - enough for nine pp5 containers
Four 1 Quart LC' Jars to be inoculated tomorrow morning

I bought a bunch of sterilite today, including a 120 litre massive clear one with super secure lid - monster mono perhaps?

The rgs will be ready in a few hours, and in the morning I will do the WBS into jars and sterilize and innoc using syringe -

Cant wait for this syringe bs to be over with and innoc using grain or lc for speedier colonization.


YAY! I am super happies :laugh:

Oh - update on the humidifier, I said fk it and bought a whole house humidifier so I can be happier throughout the house, and a humidifier for the room itself.

Oh yeah - going to make my glovebox now ( a huge tall clear sterilite  :smile: )


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19022937 - 10/23/13 11:58 PM (10 years, 3 months ago)

Oh, the feels man. I never went this far the first time, I did everything in an 8qt PC using BRF/V PF's -

Here is a picture of the closet I will be doing things in.








It was really dirty WBS - so I decided to wrap a bit of leftover screen for the sliding door I had around the pot - Worked great!


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19022939 - 10/23/13 11:58 PM (10 years, 3 months ago)

:rockon:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19023200 - 10/24/13 01:30 AM (10 years, 3 months ago)

What do you guys think ( we'll storm, since I think you're the only one following this thread ) about the LC method using 2 TBLSPNS of Light Corn Syrup Pint / 4 Quart - PCing and then 1cc of each syringe?

IT would be good for a lc injection when I get 24 more quarts next week.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19023206 - 10/24/13 01:31 AM (10 years, 3 months ago)

LCs in general suck.

Research agar and G2G.

It's much more promising.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: PussyFart]
    #19023299 - 10/24/13 02:09 AM (10 years, 3 months ago)

Quote:

Notahacker420 said:
LCs in general suck.

Research agar and G2G.

It's much more promising.




I think this ^


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19023354 - 10/24/13 02:31 AM (10 years, 3 months ago)

Quote:

Stromrider said:
Quote:

Notahacker420 said:
LCs in general suck.

Research agar and G2G.

It's much more promising.




I think this ^




Oh, I dont doubt this, thats why I've got a box of 125 presterilized 3 tier petri dishes, hundreds of syringes, a scalpel on the way ( old vintage one because its so retro its not funny ) the material list building up for the creation of a very large flow-hood - stainless steel table - surgical masks and other stuffs :wink:

I will be creating monocultures, and thats what the 120 litre tub is going to be for, my firt monoculture bulk grow when im ready..

BUT - Until than , I'll need plenty of culture to innoc my grains with, and I wont have a good prints to select from until I begin fruiting :wink: so, I'll be doing that along with g2g to innoc my jars.

I will be so far agars ass I will be named Sraga.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19023361 - 10/24/13 02:34 AM (10 years, 3 months ago)

You don't need plenty of culture to inoculate your grains with. Just inoculate a couple grain jars and then do g2g to expand your culture exponentially. G2g is really fast. You'll love it


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19023466 - 10/24/13 03:25 AM (10 years, 3 months ago)

Well the liquid culture right now is my guess at the only backup I'll have once innoculating all my jars for a fallout plan if all of them are contam or bad procudure... ya know first time back, work out the kinks -

Is there anything else you think I should add - with the products below mentioned, how long do you think my supplies will last, and what method would you do considering what I have avaliable to me, mention teks or personal preference - 5 shrooms to all help in this thread, I guess its my only way of giving back :/

I have 60cc's as noted ( many, many posts ago ... lol )

6 10cc Liquid Syringes 4 Different "Species"

Here are my supplies in-home as of today - I'll be growing some nice edibles, maybe shitakes or something along those lines... :mushroom2:

60'ccs of Liquid Syringe ( one looks cloudy, will post picture in a moment... It was the freebie they sent along with the purchase )

1 23 QT Presto Pressure Cooker

44 1 Pint Ziplock Twist n' Lock Clear PP5 Plastic Containers
40ish WM Ball Quart Mason Jars ( 12 Currently with 1 cigarette sized hole for  Vent and Syringe Sized Hole for Innoc

2 99L/105 QT Clear Sterilite
1 120 Qt Clear Long/Tall Sterilite
2 56qt Clear Sterilite
2 66qt BLUE ( OLD ) Rubbermade
3 Small Shoebox Sized Cheap $1 Clear Totes from HomeDepot (GreatBuy!)

1 66 QT " Virtually Indestructable Top Quality Rubbermade Rougneck Clear Container Intended for Glove/SAB

100# of WBS at 17$/50# ( Contains Small Amount of Corn and SF Seeds)
10# Rye Grass Seed from Local Feed
Gysum Via Powdered Drywall / Drywall Itself

125 3 Section Pre-Sterilized Packaged Peteri Dishes
56Grams 100% Pure Agar-Agar Powder
Disposable Latex Gloves

2 Tyvek Suits Intended for Lid Contam Barriers ( Cut one up so far )

2 Long Tyvek Sleeves ( Shoulder Length ) Cut from Tyvek Suit

2 Qts 91% Isopropyl Alcohol
1 Spray Bottle Dedicated to Alcohol
3 Large " Great Value " Disinfectant Spray

1 Cool Mist Humidifier New
1 Warm Style Humidifer *old*
1 Whole Home Humidifier *OTW*

Working Area 8x4 Walk In Closet w/ Secretarys Desk Inside (no airflow) carpeted, considering layering all walls and floor with plastic sheet ( dexter style ) could be used as sterile-ish work area for glove/sab or a fruiting area due to ease of creating high humidity.


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Edited by JMcDoogle (10/24/13 04:00 AM)


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19023491 - 10/24/13 03:40 AM (10 years, 3 months ago)

Edit your post and remove the vendors name please.

Then read the forum rules.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: PussyFart]
    #19023495 - 10/24/13 03:41 AM (10 years, 3 months ago)

Quote:

Notahacker420 said:
Edit your post and remove the vendors name please.

Then read the forum rules.




Vendor is a shroomery vendor? Didnt know I couldent use their name. Post edited to clarify intentions.

All due respect, hail the might hacker :bow2:


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Edited by JMcDoogle (10/24/13 03:56 AM)


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19023512 - 10/24/13 03:50 AM (10 years, 3 months ago)

That's just hacker. Straight to the point.  He's a good guy. I'd vouch for him any day! :smile:

He's earned a lot of respect in mush cult so you must :bow2:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19023516 - 10/24/13 03:52 AM (10 years, 3 months ago)

Post edited previously reflecting my anti authoritarian views when we all know its an necessity.


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Edited by JMcDoogle (10/24/13 03:59 AM)


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19023517 - 10/24/13 03:53 AM (10 years, 3 months ago)

Hey hey mcdoogle's 100th post :dancer:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19023520 - 10/24/13 03:55 AM (10 years, 3 months ago)

Oh and Btw it's not about being a mod. It's about protecting this community that we all enjoy having

Edit: I'd have said the same. He just beat me to it


Edited by Stromrider (10/24/13 03:56 AM)


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19023533 - 10/24/13 04:07 AM (10 years, 3 months ago)

I have 2 suggestions for your list.

1. Get a really tall clear bin for your sab. I made my first one out of a 66qt sterilite and I ended up having to scrap it because it wasn't quite tall enough to do g2g transfers properly. I'll post a pic ofmine when I get hhome. I'm at work using my phone right now

2. You want 70 percent alcohol not 90


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19023548 - 10/24/13 04:23 AM (10 years, 3 months ago)

Have you ever considered that you might be obsessive about your new beginning? I think it is fantastic, and Im laughing with you on this one. If I tried to go that far balls deep my wife would definitely make me shut it down.

  I have to say that I don't think I would use the Warm/Humidifier. This is going to breed many molds.

Nota says "Lc's in general suck."  This is because there is now real way to test for contaminates in you culture. The only good way is to take it from a LC and move it to agar. Then sector out the clean isolate until you have a perfect specimen to make a master grain jar.

  Use that jar to g2g a big whore fest of jars.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Sockadin]
    #19023561 - 10/24/13 04:29 AM (10 years, 3 months ago)

Quote:

Sockadin said:
Have you ever considered that you might be obsessive about your new beginning? I think it is fantastic, and Im laughing with you on this one. If I tried to go that far balls deep my wife would definitely make me shut it down.

  I have to say that I don't think I would use the Warm/Humidifier. This is going to breed many molds.

Nota says "Lc's in general suck."  This is because there is now real way to test for contaminates in you culture. The only good way is to take it from a LC and move it to agar. Then sector out the clean isolate until you have a perfect specimen to make a master grain jar.

  Use that jar to g2g a big whore fest of jars.




lol I understand I may be a bit obsessive in general, but what are you pointing at specifically? Materials - Attention to detail.. lol Im confused.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19024098 - 10/24/13 06:16 AM (10 years, 3 months ago)

Here is my Still air box


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19024189 - 10/24/13 06:50 AM (10 years, 3 months ago)

Hmm - Okay, Im mixing up some more WBS to do tonight when I get home - About to Simmer and Can - PC the ones I did last night using Franks procedure.

I'll be doing Strom's Start to Finish Bulk Tek with these jars, atleast the beginning stages :wink:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19024206 - 10/24/13 06:55 AM (10 years, 3 months ago)

:rockon:

Good night mcdoogle I'm off to bed


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19024478 - 10/24/13 08:44 AM (10 years, 3 months ago)

Well, I just innoced my Quart LC Jars - 1cc each - We will see how it goes in a few days - Will be Innocing my PP5 Plastics here as soon as they cool down - probably going to nap before work. Will keep you updated :wink:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19028756 - 10/24/13 10:38 PM (10 years, 3 months ago)

inoculated my PP5' Ziplock Containers ( RGS Violets Method ) before work - currently simmering about 12qt's worth WBS using FrankHorrigans generic tek


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19029236 - 10/25/13 12:50 AM (10 years, 3 months ago)

And the WBS has gone into the PC -

I want to say this, WITH discretion being I just had to deal with it -

But NEVER AGAIN - SO MESSY >.<

Bird... Seed... EVERYWHERE

IN MY SHOES


Doesent help I have 5 - 5 week old kittens watching and playing with every kernel, nearly stepping on them left and right and spilling stuff...


Oh - I'll remember this.. I will not forget.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19029250 - 10/25/13 12:57 AM (10 years, 3 months ago)

Turns out I have way more birdseed than I can fit in ten quarts, Is it advisable to open up PC' with haste * as is to relief the pressure manually, as to open it faster, and than insert another batch? I want to get them in jars and pc'ed before it dries out...


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19029363 - 10/25/13 01:56 AM (10 years, 3 months ago)

I would advise against it. Quick pressure drop can cause jars to crack. Just load the extra wbs in jars and leave it sitting until tomorrow. It won't dry out


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19033489 - 10/25/13 10:23 PM (10 years, 3 months ago)

What is the quickest one has seen growth in a jar / bag innoculating with syringe?


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19034164 - 10/26/13 03:47 AM (10 years, 3 months ago)

About 3 or 4 days is the quickest you'll see growth. It's usually closer to a week though


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19037125 - 10/26/13 07:00 PM (10 years, 3 months ago)

So i've got everything in the walk in closet -

All my jars that I have inoculated within the last three days - ten more in an hour or so ( will be 24 hours since I took off PC )

Thats all I will be doing, as to say 4 - 6 syringes are now down 3-5cc's each, I want to save them and use the RGS for g2g.

I spray the Disinfectant for a two-three seconds every time I leave the closet, it remains at a constant 81.5-82'f and there is a 6500K Daylight Compact Flourescent lightbulb running 12 on 12 off -

In your professional opinions, is this ideal?


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19037155 - 10/26/13 07:05 PM (10 years, 3 months ago)

Spraying disinfectant is pointless.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: abltsandwich]
    #19037166 - 10/26/13 07:06 PM (10 years, 3 months ago)

Quote:

abltsandwich said:
Spraying disinfectant is pointless.





Oh, lol whys that because i'll definatley stop, smells like a hospital up in there :/


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19041504 - 10/27/13 04:05 PM (10 years, 3 months ago)

Will update OP -

7 of 8 PP5 Containers show noticable growth - inoculated in glovebox using syringe on 10/25 around 5pm

The LC jars inoculated on 10/24, hard to tell, I think I did Tablespoons of Karo instead of TSP so the liquid is very dark, a flashlight does not help either. We will see in a week or so whether or not they will be viable.

inoculated ten quarts ball jars on 10/26 early midnight and 10/26 mid-day

We will see how they look here in a few days.

The pp5 RGS is 2 - 1/2 pints of each variety I have ordered, and one will be used for g2g, and another will be fruited for spore prints to go onto agar.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19041538 - 10/27/13 04:17 PM (10 years, 3 months ago)

Here are a few photos - Will add more later.


This is the above average growth of the 7/8 pp5 containers, others show a bit less growth, but still visable.




These pictures (2and3) detail the small black dots inside of the spore syringes that have made their home on the very edge of the containers, showing the spread of mycellium from them. The last picture is the jars of birdseed 45 minutes after pressure was released, immediatley after opening the PC. They did dry out a bit more to what I believe is optimal moisture content.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19042967 - 10/27/13 09:56 PM (10 years, 3 months ago)

Will Update OP

10/27 Midnight -

The WBS jars that were inoculated on 10/25 at 5pm are showing huge growth spurts - accidentally knocked heater, was only 80 in there for the last few hours im assuming, will turn up a smidge for 81'.

5/7 10/25 1QT WBS inoculated with Thai and EQ show great growth, almost more spread/depth to the mycellium than the RGS ( rye grass seed ) inoculated on 10/25 around 5 AM ( 12 hour difference ) the only difference is that due to the small containers, I may have injected less, and the big difference is that these containers have no fresh air flow, no ventilation, completley sealed - dont know that will result.

My four LC' are doing well, 2 of the moreso than others, 1 looks amazing, like I threw a bunch of cotton candy fibers in there and spun it around.

For Only three days inoculation Period with Spore Syringe - I am happy with the growth, two of the pp5 rgs have growths of over  3/4sqinch and the other not bad - the WBS growth seems to ahve run down the side of the jar, and is spreading evenly in 1-3inch spaces.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19043162 - 10/27/13 10:37 PM (10 years, 3 months ago)

I may be able to shine some light into one of your questions regarding the pricing for tubs and possibly an idea for a glove box.

I've found some fairly cheap tubs at TARGET. $10 or under depending if their running a sale, I don't use clear tubs because I've found they crack very easily if you don't use the right tools. The closest thing I ever found for cutting holes was a knife and that seems to slice right into the opaque colored tubs without a problem.

If I had a drill and slip on piece for cutting holes I'd get the clear ones.

You can construct a very economical glove box using a tub, slice two arm sized holes into it and tape some trash bags in there. Cut a large window into the lid of your tub and tape some plastic wrap over it. There you go! 'Poor Fuckers Glove Box' Tek:thumbup:

Oh shit, didn't even realize this thread was on its 4th page. Here I was thinking you were just starting out and I'm posting this shit well after you've started.


Edited by KittyKat (10/27/13 10:39 PM)


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19043364 - 10/27/13 11:27 PM (10 years, 3 months ago)

As an experiment I will be shaking one of my 1qt WBS jars that have just shown growth as of yesterday around midnight, I have three of them in nearly identical growth paterns currently (1/4inch growth, about thumb sized)

As an experiment regarding this post
http://www.shroomery.org/forums/showflat.php/Number/17924122#17924122

and my curiosity brought out by this post
http://www.shroomery.org/forums/showflat.php/Number/19043090

I am going to go upstairs and shake one of my jars -

We will see what happens.

All jars are numbered, and logged every day for signs of mositure content, growth, and any signs of contamination, approx growth site, localized or random, ect.

Business as usual.

Shook QT#4(MALABAR) which had similar growth to QT#? ( I forgot, its written down though. )

Both were inoculated same minute, reside in the same room at the same temperature, shown similar signs of growth - one is shaken, the others remain untouched.

Will keep updated on this side-experiment.


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Edited by JMcDoogle (10/27/13 11:43 PM)


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19044289 - 10/28/13 04:12 AM (10 years, 3 months ago)

Im going out on a limb here, but I too have decided to give plastic a try.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Sockadin]
    #19044302 - 10/28/13 04:16 AM (10 years, 3 months ago)

Three days, and 8 of my pp5 plastics show quarter sized growth. inoculated from spore syringe from sponsor.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Sockadin]
    #19044309 - 10/28/13 04:19 AM (10 years, 3 months ago)

Quote:

Sockadin said:
Im going out on a limb here, but I too have decided to give plastic a try.




Im sure unsure of whats going to happen when it runs out of air to consume, seeing as after sterilization and inoculation the jars are shut tight, I believe reading in violets tek you can open the jars a quarter turn and the pressure inside will level out in some way, ill look up details on the heresay.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19044357 - 10/28/13 04:45 AM (10 years, 3 months ago)

I was thinking about it, why couldn't we modify the top of the jars and install a synthetic filter disc as a breathable barrier for gas exchange?  I mean it is too late for your jars, but I might try it out. Also if your jars start to stall which they probably wont, just crack the cap.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Sockadin]
    #19044424 - 10/28/13 05:30 AM (10 years, 3 months ago)

Yep, I agree, but I dont think they'll stall, seeing as I've seen like fourty in one area on one of violets post and he/she does not use a breathable lid...

:laugh: Going to go check out the growth, its amazing what happens in a few hours.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Sockadin]
    #19044617 - 10/28/13 07:50 AM (10 years, 3 months ago)

Quote:

Sockadin said:
I was thinking about it, why couldn't we modify the top of the jars and install a synthetic filter disc as a breathable barrier for gas exchange?



You could do this. I recommend against it though. Silicone falls off of these lids quite easily. My first uses of these containers was done that way – it annoyed the shit out of me to sterilize a round of containers, inoculate them, and then have the filters fall off a bunch of them on accident.

Quote:

Sockadin said:
Also if your jars start to stall which they probably wont, just crack the cap.



Yep!

A "quarter-turn" is far too much. It only takes 1/8th turn to pop the lids off.
If necessary, loosen just 1/2-1 inch of the lid circumference (which is like 15-16 inches)

Quote:

Violet said:
I've done hundreds of these and full colonization has always been reached without the need for any gas exchange.
Perhaps with an agar wedge inoc of a not-so-speedy or multi-spore culture you could experience stalling before full colonization. If this occurs, only a slight crack of the lid is necessary to allow excess CO2 to be pushed out without compromising the rest of colonization.




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Edited by Violet (10/28/13 09:11 AM)


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Violet]
    #19044621 - 10/28/13 07:55 AM (10 years, 3 months ago)

Violet I have had trouble getting silicone to stick to my plastic jar lids before. I started scuffing them up with a rough grit sandpaper and it helped a lot. I imagine the it would work good for the plastic containers you use as well. Silicone just doesn't stick to a slick surface very well. It needs some nooks to grab onto


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19044786 - 10/28/13 09:10 AM (10 years, 3 months ago)

Yeah I've read where people got it to work out by roughing-up the surface silicone is to be applied to.  I didn't have to do this for my plastic quart jar lids but it definitely would have been necessary for the container lids.
My preference is to not modify (maim) them at-all. They almost never need gas exchange before fully-colonized and can be sterilized loose-lid so there's no need to allow the moisture loss thru a filter.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Violet]
    #19044809 - 10/28/13 09:18 AM (10 years, 3 months ago)

I could definitely see the moisture loss being an issue. Sometimes when I inoculate a new grain master with an agar wedge it takes a while to colonize. The grains on top dry out and don't want to colonize


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19056259 - 10/30/13 05:15 AM (10 years, 3 months ago)

My fist experience with these 5pp plastic bowls was interesting. I want to say after I burned my WBS and the whole house stunk, the RGS did well in the microwave. But now they look like they might be getting a little too dry, its hard to tell.

Also anyone ever have an issue with RGS and weevils? I got a package of RGS, and I swear there was a bunch of dead weevils in it. Luckily they are floaters when dead, so I got them all out.

I just read that they lay eggs in grain batches. Should I toss all of this RGS, or do you think the microwave did the trick?





Edit: Spelling


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Sockadin]
    #19056296 - 10/30/13 05:36 AM (10 years, 3 months ago)

Hmm, Im not sure I would throw it away if it were me, but thats me and Im not too experienced.

I would have just PC'ed it and then not cared about the eggs, but I donno about microwave.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19057036 - 10/30/13 11:09 AM (10 years, 3 months ago)

Any update for those jars you shook, did they slow down?


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19057728 - 10/30/13 01:51 PM (10 years, 3 months ago)

Quote:

MUSH HEAD420 said:
Any update for those jars you shook, did they slow down?




I'll go do a side-by-side right now for ya :wink:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19057975 - 10/30/13 02:36 PM (10 years, 3 months ago)



Heres some updated pictures.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19058109 - 10/30/13 02:58 PM (10 years, 2 months ago)

Shaking appears to have been ok for you ! Although all I read says it's hit or miss. When I saw your post about running upstairs to shake I did the same lol the jar I shook had a pea sized growth but alas the very next day there was 2 bb sized green dots and it has been destroyed :sad:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Rubestoad]
    #19058145 - 10/30/13 03:02 PM (10 years, 2 months ago)

Quote:

Rubestoad said:
Shaking appears to have been ok for you ! Although all I read says it's hit or miss. When I saw your post about running upstairs to shake I did the same lol the jar I shook had a pea sized growth but alas the very next day there was 2 bb sized green dots and it has been destroyed :sad:





What was your contam barrier like? Tyvek? Disks?

Im curious as to how the contam was presented, perhaps it was on the interior of the jar and when you shook you brought it out.

If you're confident in your contam.barrier on the jars, than I would keep an eye out on the rest.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19058165 - 10/30/13 03:07 PM (10 years, 2 months ago)

I'm using tyvek but I'm pretty sure my syringe is the culprit I'm very new and I posted just a bit ago about my start called introducing me and what I'm doing.

Sorry I'm not even sure how to post a link to it :confused:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Rubestoad]
    #19058447 - 10/30/13 03:54 PM (10 years, 2 months ago)

How good of a shake did you give it? As you probably notice it is about the same % of colonization if not more because of the fact that you can't see through to the middle.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19058465 - 10/30/13 03:57 PM (10 years, 2 months ago)

I shook it once , but more of a spin, err..


After I gave it one good shake I held it sideways and rolled it, as to gently distrubte rather than beat against the sides of the jar as in shake.


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19058508 - 10/30/13 04:07 PM (10 years, 2 months ago)

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Edited by MUSH HEAD420 (10/30/13 04:24 PM)


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: MUSH HEAD420]
    #19058586 - 10/30/13 04:27 PM (10 years, 2 months ago)

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Edited by MUSH HEAD420 (10/30/13 04:28 PM)


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19058624 - 10/30/13 04:36 PM (10 years, 2 months ago)

We'll see how it goes in the long run, could perform faster here, but turn out to be a dump in colonization when g2g is done/fruited.


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19058716 - 10/30/13 04:52 PM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19063711 - 10/31/13 02:19 PM (10 years, 2 months ago)

My shaken jar is now  30-50% colonized depending on the interior.

Im doing all this thinking on how im going to take my 16 qts and 8 pints to fruiting, mini mono alongside monster mono two regular 56 qt monos using 7 qts a piece, omg -

Im getting psyched...

:fuckyeahdance:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19063721 - 10/31/13 02:21 PM (10 years, 2 months ago)

Oh, and when I get home I'll begin the agar - just gatta grab a wine bottle from work tonight :smile:

:justcantwait:


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Edited by JMcDoogle (10/31/13 02:28 PM)


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19064207 - 10/31/13 03:58 PM (10 years, 2 months ago)

Your going to love playing around with agar. It's my most favorite thing about cultivation


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19065516 - 10/31/13 07:36 PM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19066870 - 10/31/13 10:55 PM (10 years, 2 months ago)

Quote:

MUSH HEAD420 said:
Quote:

JMcDoogle said:
My shaken jar is now  30-50% colonized depending on the interior.






Same here, the experimental shaken jar is going to be done so soon ... put it in the fridge and wait for the others to catch  up?



Quote:

MUSH HEAD420 said:
Quote:

JMcDoogle said:
My shaken jar is now  30-50% colonized depending on the interior.






Same here, the experimental shaken jar is going to be done so soon ... put it in the fridge and wait for the others to catch  up?




Even though it was one of the slower ones when I iniatially shook it, Im looking at it now, I think it'll be done in four days max - the others look 4-5 days away from 30-40%...

As I said, even though it was the slower one from the start, after the shake it took off like a bitch out of hell and I think im going to use that one for a g2g...I think it was EQ or Thai Lipi, forgot.. its all logged though -


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19066999 - 10/31/13 11:18 PM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: MUSH HEAD420]
    #19067176 - 10/31/13 11:57 PM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19067207 - 11/01/13 12:03 AM (10 years, 2 months ago)

Quote:

MUSH HEAD420 said:
5 jars, from 10%-20% were just shaken. We will see if any of them stall or contam.





I shook a few that were at 10-20% too last night - guilty of being impatient, but I have the same number unshaken - we will see the results here within a few days.

Agar is in the PC right now. I've decided to go forward in regards to the last post.


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19067222 - 11/01/13 12:06 AM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19067268 - 11/01/13 12:14 AM (10 years, 2 months ago)

Quote:

MUSH HEAD420 said:





omg, it took nearly .2 seconds before I was in a fit of laughter,

that made my day.


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19067355 - 11/01/13 12:27 AM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19067826 - 11/01/13 02:49 AM (10 years, 2 months ago)

Well, I just poured probably 11-12 plates full of 3 section agar -

the consistency didnt look right, perhaps it got too hot, too long.

We will see in a few hours when it cools.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19073087 - 11/02/13 12:31 AM (10 years, 2 months ago)

Will update OP;

Note: Im having trouble uploading photos through the
boards currently, all pictures i upload are chopped
in half or more, never showing the entire image.

---
Here are the pictures of the shaken vs non jars.
This experiment is showing some profound results
as of now. We will see what happens in the fc -

I went out and bought 3 6-8qt sterilites for
mini-monos to reflect the overall colonizaion and growth
of these three jars in their entirety.

Quote:

JMcDoogle said:





New


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19078815 - 11/03/13 04:35 AM (10 years, 2 months ago)



Updated OP -

Check the last picture, I may just be a little paranoid,
but this may be a small contam -

nontheless I shook the jar - we will find out..

What do you think?


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19078820 - 11/03/13 04:41 AM (10 years, 2 months ago)

That does kind of look like cobweb


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19078822 - 11/03/13 04:45 AM (10 years, 2 months ago)

Thats what I thought

That was my fastest jar too.

:justkillmenow:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19078828 - 11/03/13 04:50 AM (10 years, 2 months ago)

I feel your pain brother. Wait till you fuck up a whole spawn run! I fucked up 8 jars one time because my grain master had a contam hiding


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19078848 - 11/03/13 05:19 AM (10 years, 2 months ago)

Quote:

JMcDoogle said:




Quote:

Stromrider said:
That does kind of look like cobweb



:whathesaid:


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: PussyFart]
    #19079127 - 11/03/13 07:34 AM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: MUSH HEAD420]
    #19081485 - 11/03/13 05:18 PM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19083825 - 11/04/13 01:13 AM (10 years, 2 months ago)

Just was checking around the forums,
came across a post saying its easy to get
free composted manure around october in the midwest

so I checked craigslist, and within a few miles
three or four farms have composted h-poo avaliable
and fresh aswell, some from months ago -


Do you think this is a good idea opposed to
coir / verm seeing as its free?


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19084416 - 11/04/13 07:57 AM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19084527 - 11/04/13 08:42 AM (10 years, 2 months ago)

Poo works great but is not as contam resistant as coir. I like coir because it's so cheap and easy. It's almost fool proof. Almost :lol:

You have to pasteurize poo. The bucket tek will not suffice. If you properly pasteurize it then you should have no problem


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19084899 - 11/04/13 10:52 AM (10 years, 2 months ago)

That's how I just got my poo 2 weeks ago. And aged hpoo doesn't smell at all.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19088044 - 11/04/13 11:45 PM (10 years, 2 months ago)

Prepped a bunch more of WBS yesterday -

Wasnt able to find WM Quart Jars - So I just succumed to using
regular mouth jars -

24 More to PC Tonight -

another 24 More When the WM come in from order.


Next step, bulk spawn materials
picking up a bunch of coir/verm
and h/poo straw wednesday.

Its beginning to get interesting.

Still nothing showing on the agar plates,
Im assuming thats a good sign due to no contams

Will update soon!


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19088266 - 11/05/13 12:54 AM (10 years, 2 months ago)

haha, ive nearly got my PC to the brim with bs right now

Its taking a long time to simmer, but I've rigged a huge
strainer using window screen and a large tote.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19088920 - 11/05/13 07:47 AM (10 years, 2 months ago)

An update -

I have processed a ton of quarts, pc been going
for a few hours now -

Will have a lot of g2g to do tonight

Has anyone ever injected sterile distilled water
into their fully colonized grain jar and than sucked that water
back up and used it to inoculate?

Wondering what experiences people have had with that.

Thanks for looking!


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19088937 - 11/05/13 07:53 AM (10 years, 2 months ago)

Quote:

JMcDoogle said:
Has anyone ever injected sterile distilled water into their fully colonized grain jar and than sucked that water back up and used it to inoculate?
Wondering what experiences people have had with that.



That is called "grain liquid culture" or GLC. (I think it's a poor name, I prefer to call it water suspension transfer)
It works well - as long as the master jar is entirely contaminant-free and you keep it totally clean. It can be tedious transferring all that water though, and all the needle flaming...


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Violet]
    #19088977 - 11/05/13 08:14 AM (10 years, 2 months ago)

Right on, thanks Violet -

I'll just stick to a long stainless spoon
wiped with alcohol in a SAB to do the g2g.

:smile:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19088983 - 11/05/13 08:17 AM (10 years, 2 months ago)

Quote:

JMcDoogle said:
Right on, thanks Violet -

I'll just stick to a long stainless spoon
wiped with alcohol in a SAB to do the g2g.

:smile:





idk man, personally i wouldn't use a spoon for doing g2g - i just pour straight from master jar into the receiving jars - just one less thing coming in contact with your grains, ya know?  and alcohol doesn't sterilize your spoon.....if you must do it this way with a spoon, i would PC it for best results to get it sterile......but i think just avoid using utensils here altogether - just throwing that out there, its what works for me :shrug:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: PsiLisaBin]
    #19089026 - 11/05/13 08:28 AM (10 years, 2 months ago)

Well, I figured the less I had to open the jars and the
less air movement would be more effective at combating
contams.

See when your wabbling a jar around in the SAB it
takes a lot of movement, and I've not a sealed SAB
that would induce a lot of current,

Though popping the lid slowly, and carefully manuevering
a spoon within would create less air currents.

Im sure if I soaked the spoon in alcohol, after lysoling it,
and prior to lysol I'll scrub it with a stainless steal brush.

I dunno, I'll try both methods, ive got enough jars to do.


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19089060 - 11/05/13 08:40 AM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19098294 - 11/06/13 11:12 PM (10 years, 2 months ago)

30 Jars GTG Complete

EQ,MAL,andGT

THAI to be done tomorrow.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19103185 - 11/07/13 10:14 PM (10 years, 2 months ago)

Another batch of WBS going tonight
going to have enough for a few monos -

Found everything I need at a local hydro store,
question is.. their vermiculite is horticultural grade,
it is really fine, will it work okay?

Of the 30 Jars, no contams showing up -

Though I will update a picture on one of my grain jars
that I find a bit odd looking, but smells great.

Onwards!


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19103243 - 11/07/13 10:26 PM (10 years, 2 months ago)

Post deleted by MUSH HEAD420

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19103270 - 11/07/13 10:30 PM (10 years, 2 months ago)

Quote:

MUSH HEAD420 said:
Some people here take their medium grade verm and break it up to fine. I use fine verm and think it holds more moisture.

I just do not think it is as good for casing as it tends to compact more. I'm sure the pins will not have any problem breaking though it though.

As a mix for bulk sub I would go with fine.





Great, thanks for the replies throughout the past week or so,
good to know someones actually watching.

:murray:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19103369 - 11/07/13 10:53 PM (10 years, 2 months ago)

:popcorn: we started at close,to the same time your kicking my àss lol
Keep up the good work!


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19103720 - 11/08/13 01:07 AM (10 years, 2 months ago)

Here is a photo update!



The two pictures of the single jar, I am concerned about -

It looks really heavy, albeit it may be caused by the grain shrinkage and it trying to reach out to the glass - You be the judge of that - I was going to do another ten or so jars tomorrow using that a master g2g -

Its hasnt been that long, but it looks like my original idea of having a lasting supply from a few cc's is on its way to becoming a reality - thanks to all who have helped and inspired me along the way!

Onward!


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19103752 - 11/08/13 01:27 AM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19103760 - 11/08/13 01:32 AM (10 years, 2 months ago)

Wheres NAH or RR when you need em'

:rr:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19103767 - 11/08/13 01:35 AM (10 years, 2 months ago)

I don't like the looks of that jar and would be weary of using it as a grain master


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19106559 - 11/08/13 04:31 PM (10 years, 2 months ago)

Looks like sex.
It's wet.
It's dirty.
It's Funky.
Looks good to me.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Sockadin]
    #19108919 - 11/09/13 02:51 AM (10 years, 2 months ago)

What..

Do I gatta break a rule or say something stupid for
NAH's alarms to go off?

:ninjawe:

"Did you know the reason you trip when taking mushrooms is because they are poison, your dying. Horribly"

Did you guys know that!!?!?!?!

:trollcop:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19108922 - 11/09/13 02:54 AM (10 years, 2 months ago)

AS far as an update -

My Jars are PCing currently,

10 Done, another 10 In - Probably going to go to bed after this
batch.

I'll be doing some mono's monday or tuesday.

Wait for it, wait for it...

:fuckyeahdance:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19110145 - 11/09/13 12:11 PM (10 years, 2 months ago)

Another 27 Jars G2G

Looking forward to this next week :smile:

Next up - Pasturization of Sub


2 monos at 7 Qts Spawn Each ( 55 Litre ) EQ/GT
Another at 12 Quarts Spawn( 99 Litre ) MAL


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19124034 - 11/11/13 11:17 PM (10 years, 2 months ago)

Working on Agar Tonight -

I have a few good isolates selected.


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19124052 - 11/11/13 11:21 PM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19124461 - 11/12/13 01:18 AM (10 years, 2 months ago)

Quote:

MUSH HEAD420 said:
:awesanta: what kind of traits are you trying to use? colonization speed,looks of the myc,fruits?




Dumb Luck

:dawerp:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19124467 - 11/12/13 01:19 AM (10 years, 2 months ago)

Quote:

JMcDoogle said:
Quote:

MUSH HEAD420 said:
:awesanta: what kind of traits are you trying to use? colonization speed,looks of the myc,fruits?




Dumb Luck

:dawerp:




I started with a grain from the fastest colonizing MS

Three Tier Petris

best of the three

Most Rhizo.


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19124649 - 11/12/13 02:33 AM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19124760 - 11/12/13 03:04 AM (10 years, 2 months ago)

Quote:

MUSH HEAD420 said:
I notice people try to get the rhizo growth, is there a reason for this?
I didn't think rhizo growth was preferable.

I can see taking the fastest colonizer or cloning a fruit...




You got me,

I've been told that I am a noob...

I dont think so.. I've seperated all sorts of growth,

I'll keep masters of the finals, fruit into mini monos
inside of a SGFC and than determine which has the qualities
I like the most, clone to agar, isolate one more time,
and call that MY BITCH.

:nodofunderstanding:  :hahyeahwoo:


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19124814 - 11/12/13 03:44 AM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19129009 - 11/12/13 10:32 PM (10 years, 2 months ago)

Guess whos pasturizing a bunch of bulk...

This guy :smile:

:shockandawe:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19129840 - 11/13/13 02:47 AM (10 years, 2 months ago)

5 6QT Mini Monos with 1 QT Spawn 2 QT Sub

1 12QT Mini Mono with 4 QT Sub 2 QT Spawn


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19130733 - 11/13/13 11:01 AM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19132757 - 11/13/13 07:11 PM (10 years, 2 months ago)

Great Minds Think Alike.

That is my exact plan.

Are you watching me? I'll wave.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19135206 - 11/14/13 08:55 AM (10 years, 2 months ago)

Made my SGFC and stuck a cake in there I made from leftover

g2g's that I jammed inside a plastic pp5 container and let it consolidate and it turned into a nice hard cake.

Should see some results in the next week or so.

Has anyone had any luck in the north, placing cakes in the ground
before the winter freeze and having anything pop up in spring?


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19135299 - 11/14/13 09:20 AM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19135345 - 11/14/13 09:31 AM (10 years, 2 months ago)

Their in their pp5 containers still.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19135387 - 11/14/13 09:40 AM (10 years, 2 months ago)

That is fine too, it will hopefully spend all its fruiting capabilities from the top of the sub, you won't need to worry about moisture as much as you would out of the container.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19140963 - 11/15/13 01:22 PM (10 years, 2 months ago)

Just X'fered some wild australian cube prints to agar...

:justcantwait:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19141007 - 11/15/13 01:41 PM (10 years, 2 months ago)

I have some wild Aussie prints too and have worked a bit with them before.  If your experience is to be anything like mine, expect to work with them over some spore generations before you start easily seeing results like you want.  No awesome cultivar genetics ever showed up for me, so instead of focusing on bringing out traits I wanted over many grows, my attention drifted elsewhere.
But you could have lots of fun so 'domesticating' a wild spore line yourself!


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Violet]
    #19148132 - 11/17/13 01:10 AM (10 years, 2 months ago)

Starting another batch of spawn -

I think 1 Brick Coir / 2.5 Qts Verm / 4.5 Qts Water Give or Take. x 2

As always, proper pasturization using Candy Thermometer for 90 mins at 135-150'f

Even if not completley accurate, I believe in so doing this would
achieve better results than the bucket tek.

BTW, I have cats. I am using leftover Tidy Cat Kitty Litter
Containers, Very Sturdy Plastics with a Lid.



They Work Wonders for this. Just Food For Thought!


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19148235 - 11/17/13 02:17 AM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19148268 - 11/17/13 02:35 AM (10 years, 2 months ago)

Dont laugh Mush,

No.. you're going to laugh.. Or Cry..

But...



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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19148271 - 11/17/13 02:36 AM (10 years, 2 months ago)

Can I say.. No Ziplock Waste?

:aweohyou:


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19148293 - 11/17/13 02:50 AM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19148774 - 11/17/13 08:46 AM (10 years, 2 months ago)

I turned my kitty poo litter box pasturized sub into a big mono

heres a picture of that,

and also I've got a few 16 qt and 6 qts mini-monos started aswell.

going to do 2 110 qt's in a few days.

I have 36 Qts of spawn going currently...

time to go buy more wbs, im out. jars are being freed up by the day,
its going to be time for another PC run here next week.



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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19151321 - 11/17/13 07:07 PM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19151531 - 11/17/13 07:58 PM (10 years, 2 months ago)

Quote:

MUSH HEAD420 said:
Quote:

JMcDoogle said:

going to do 2 110 qt's in a few days.




:awesanta:

Do you put a top layer of coir on top, mix it all, or ice your monos? just wondering.




This picture is from around 5 am this morning,

At the time of the picture they are only around 3 1/2 days since inoculation.



The side by side.

One has a top layer of sub,

The other does not.

You can see the difference.


On the ones I did today, I did not use any top layer.
Only mixed up the grains really well with my hands ( in a glove )


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OfflineStromriderM
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19153151 - 11/18/13 08:16 AM (10 years, 2 months ago)

Yeah screw that top layer. I had to learn that lesson first hand as well. I never use a top layer and my 66 qt tubs are always colonized in less than a week. That's using 6 qts of spawn to 10 qts bulk sub


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OfflineJMcDoogle
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19154560 - 11/18/13 02:51 PM (10 years, 2 months ago)

Made a nice big SGFC and stuck a bunch of left-over grain from
g2gs inside a small pp5 container, let it consolidate,

and well..

My first pins in years!

:smile:



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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19155662 - 11/18/13 05:34 PM (10 years, 2 months ago)

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OfflineJMcDoogle
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19155704 - 11/18/13 05:41 PM (10 years, 2 months ago)

Quote:

MUSH HEAD420 said:
So is that grain with a layer of coir... Idk what I'm lookin at

tell me your process please, I tried to fruit an early jar by putting it in a pp5 alone, consolidate the grain then plop it on the lid and put it in the sgfc cased with verm on top and bottom... it was a nice solid cake of grain and mycelium but it did nothing but sit there and never pin. :shakefist:




So after I did my g2gs I had like an inch or two left of grain
and I didnt want to re-open a jar to dump it in, so I just put the
lid on it and forgot about it for a few days. Than I was remembering
Violets Thread and her casing of top-soil she uses on her RGS. So I
just went outside and grabbed a bit from a bag in the garage and plopped it on there, no sterilization or anything. I really didnt
think anything would happen. I got it wet, which turned the white myc
dark in a few areas from it draining into the pp5, but after a few days the myc ate that up and it went back to white. I left it above
my stove with the top only on slightly for like four days, and when
I went to the store for more coir a couple days ago I picked up some
perlite, made a SGFC out of a 110QT Sterilite. I put no light on it
or anything, it was just in front of a window, and its been really
dreary here the past few days. I about shit when I saw the Hyphal Knots... So now I took an old snake-light and put a 6500k bulb in it
this morning and its on during the day with the sun, and off when it gets dark.

We'll see what happens.

Sorry for the story. Im not in the mood for simple, technical info :wink:


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OfflineJMcDoogle
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19158464 - 11/19/13 06:49 AM (10 years, 2 months ago)

Wow, this little bugger is pinning heavily.

It helps when your spore vendor has been in business for like 20 years,
even their MS is awesome :wink:

Jk. I know that has nothing to do with it.

If I have as good luck with no contams with my bulk, and the
pinset i've achieved on this tiny 1/2 wbs cake... I'll be one
happy guy.


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19159666 - 11/19/13 01:24 PM (10 years, 2 months ago)

Post deleted by MUSH HEAD420

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Edited by MUSH HEAD420 (11/19/13 02:02 PM)


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OfflineJMcDoogle
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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19161975 - 11/19/13 08:48 PM (10 years, 2 months ago)

Nice work Mush.

I'll be trying a new filter out next grain run.

Heres some info from a post I've made on another thread.



This is called EcoFelt, EcoFi, FriendlyFelt, amongst many other names.

It can be used in place of SFD's and can be PC'ed many times
before degradation becomes a factor.

I picked up three sheets for five dollars today at a fabric store.
I should've gotton all white, I dont know what I was thinking.
:shrug:

$1+/- for thirty, fourty, fifty SFD replacements
depending on the size you choose to cut out.

It is highly cost effective for the frugal, and has
proven to work very well. It is not true felt, this is a polyester
fibre smashed together, same thing as Polyfil, or the stuffing you
pull from your pillows to filter contams from grain jars. If it can
protect sterile grain from contams in a fluffy state, why not in a
flat-mash paper style?

You be the judge.


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19175966 - 11/22/13 06:32 PM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19175974 - 11/22/13 06:34 PM (10 years, 2 months ago)

Clones from ms are not bad...tranferring from them can be


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: cronicr]
    #19175987 - 11/22/13 06:39 PM (10 years, 2 months ago)

Where else would you get a clone from anyway lol, no need to clone an iso


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I'm tired do me a favor


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: cronicr]
    #19175993 - 11/22/13 06:40 PM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19176005 - 11/22/13 06:44 PM (10 years, 2 months ago)

Yep


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I'm tired do me a favor


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: cronicr]
    #19180280 - 11/23/13 07:48 PM (10 years, 2 months ago)

Oh boy, OHHH BOY.

Things are looking fantastic.

:prettyflyforawhiteguy:


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Re: Need Help Sailing through Endless Skies - Starting Up Again *DELETED* [Re: JMcDoogle]
    #19180359 - 11/23/13 08:06 PM (10 years, 2 months ago)

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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19180385 - 11/23/13 08:15 PM (10 years, 2 months ago)

Since it's kind of like polyfill, could you use that for FAE instead of polyfill when fruiting?


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: MUSH HEAD420]
    #19182705 - 11/24/13 01:39 PM (10 years, 2 months ago)


Quote:

MUSH HEAD420 said:
So I could take a wedge of colonized agar from a MS clone, make a liquid culture with it, then inoculate grain to spawn to bulk?

Would the resulting fruits be similar to the clone?




Some consider LC unreliable. Instead you could put the wedge in a small bit of grain in a small jar with SHIP in lid. Clean water is injected after colonization and the mycelium shaken loose, using that water suspension as inoculum. This allows any contams to show easily unlike LC. It is usually not a problem with an agar wedge inoculation but the suspension method is more reliable/faster
Also known as grain LC


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Edited by mushrume man (11/24/13 01:52 PM)


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: mushrume man]
    #19211547 - 12/01/13 08:43 AM (10 years, 1 month ago)

Well, in the week since my stupid Ban Lottery Win a lot has happened -

3 56 Monos are into fruiting conditions and my 110 mono is nearly fully colonized

I have six-six quarts fruiting canopys & three 16 quarts pinning.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19211563 - 12/01/13 08:50 AM (10 years, 1 month ago)

I was wondering what happened to you. :lol:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19234226 - 12/05/13 10:12 PM (10 years, 1 month ago)

Wow, so a small update.

Been super busy with work, and I've come to a albeit late relization that I've
slightly over-done this...

Anyways, here are a few cool photos for your viewing pleasure.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19234276 - 12/05/13 10:23 PM (10 years, 1 month ago)

And two more -



This is a picture of a small mini wbs/coir cake I've set into fruiting conditions,
and a large 110 qt mono coming out of colonization.


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19234286 - 12/05/13 10:25 PM (10 years, 1 month ago)

Quote:

JMcDoogle said:




Giggity!


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: PussyFart]
    #19234293 - 12/05/13 10:27 PM (10 years, 1 month ago)

Great work .

Quote:

Notahacker420 said:
Quote:

JMcDoogle said:




Giggity!



Alright !!


Cyn


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: TheCyndicate]
    #19234382 - 12/05/13 10:51 PM (10 years, 1 month ago)


:fuckinawesome:

I think I am going to take what i've learned over this last month and
apply it to a more legal avenue on a larger scale - I just wanted to thank
everyone for their contributions, advice, criticism and all else. As you can
see I've loaded up pictures I am not proud of ie: the massive side and bottom
pinning from my first fruited 56 qt monotub. I wanted to show some of the bad
along with the good.

I also did all of this, ALL 48 quarts
of spawn using MS without isolates and it could've turned out a lot better, but
I wasnt expecting 0% contam rate and 100% fruiting, so i've got a lot of work
cut out for myself, the fruits may not be very large but my god are there a
lot of them and with this increasing paranoia :wink:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19234668 - 12/06/13 12:24 AM (10 years, 1 month ago)

:heytherebadboy:  :rocketcrotch:  :prettyflyforawhiteguy:  :greatjob:  :asianofapproval:  :seriousthumbsup:


that looks good and its from ms. good job


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: silverstem]
    #19236253 - 12/06/13 12:04 PM (10 years, 1 month ago)

Thanks everyone!

Yeah, Its kind of a pain, my fingers are getting scissor blisters :wink:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: JMcDoogle]
    #19236725 - 12/06/13 01:58 PM (10 years, 1 month ago)

You've not over done it. You're going about it perfectly. Now spread the love with all your friends :smile:


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Re: Need Help Sailing through Endless Skies - Starting Up Again [Re: Stromrider]
    #19248573 - 12/09/13 02:47 AM (10 years, 1 month ago)

I think I remember saying something about the insanity of your ambitions before. Nice Job SiR!!!
You get a Big Thumb..:thumbup:


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