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OfflineBeppe
Stranger
Registered: 09/19/13
Posts: 31
Last seen: 10 years, 2 months
Half my batches don't germinate
    #18991943 - 10/17/13 05:23 PM (10 years, 3 months ago)

method:
14 steam sterilized 1/2 pint PF tek (2:1:.8, verm:brf:50/50 water/brewed coffee)
3 steam sterilized .5L whole rice tek (3:1, whole brown rice cooked in 50/50 water/coffee:verm)
4 MS syringes (golden teacher, ecuador)

I followed the teks to the T. Used open air inoc on half of all jars/half liter glasses, and half in a GB. I used 4 different GT ms syringes throughout the entire time, and 1 Ecuador.

I had about 5/17 diff projects work flawless, the rest all showed 0% germination let alone myc growth. It's not the syringes, or the teks. My sterile conditions are optimal in general, and I have had no contams (sorry bout my first post it was just clumpy wet BRF, I have come to recognize it over time and this is why I cut my water atleast 20% for the PF recipee).

So WTFFFFF WHYWOJTTMUFNDK SPORES FUCKING GERMINATE lol. It can't be every cake/cup I knocked up was too hot. Any other ideas?

Btw pat on the back to myself for going 10+ trials with no trich or anything at all gross without a PC, and congrats to all of your communications skills for making this info available in such abundance.

No way this site isn't 24/7 monitored


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OfflineMidnight Cyclone
StrangerDanger
Registered: 05/29/13
Posts: 399
Loc: oo ess aye oo ess aye Flag
Last seen: 10 years, 11 days
Re: Half my batches don't germinate [Re: Beppe]
    #18992053 - 10/17/13 05:47 PM (10 years, 3 months ago)

Quote:

I had about 5/17 diff projects work flawless, the rest all showed 0% germination let alone myc growth




Clarify this for me? The 5/17 projects. Are you calling each jar a project, or are you saying you failed to get any colonization what so ever in any of the 14 jars on 12 out of 17 different grows? Ouch.

Out of those 5 that were successful, were all the jars colonized 100% and went through fruiting?


Quote:

It's not the syringes, or the teks. My sterile conditions are optimal in general, and I have had no contams




How can you be sure it's not the syringe?
Sterile condition are optimal in general? What does in general mean?


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