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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 3 months
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Agar to MycoBAG? Possible?
#18991350 - 10/17/13 02:56 PM (10 years, 3 months ago) |
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Okay just a simple question. I inoculated 2 spawn bags of wheat berries with some MS around 2 weeks ago. One of the bags just barely shows some colonization, very tiny dots of mycelium, so I'll leave that one since its showing some signs of growth. And actually I just dropped the bag so now I can't even see those tiny grains with small growth haha.
I was wondering if anyone has ever opened a grain bag in a glove box and dropped a piece of agar in there? I mean I could just make a jar of grains, but I find it too be a pain for one jar.
I see my options as
A.) Leave the grain bag and wait for it to show growth
B.) Re-Shoot up the bag with more spore solution. ( Not a fan of this choice)
C.) Transfer an agar wedge to the bag in a glove box and tape up the hole.( I dont have an impulse sealer)
D.) Just forget about the bag and wait to use this agar on clean jars that I know I can control.
PS the bags are from out-grow! Which I must say he has great bags and the filter patches are great. He sent me some freebies to test them out. Thanks guys!
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Plenty of people inoc bags with agar wedges.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 3 months
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Re: Agar to MycoBAG? Possible? [Re: maddchef]
#18991418 - 10/17/13 03:13 PM (10 years, 3 months ago) |
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I know that plenty of people do, they usually have a flow hood though, which I stated I DO NOT. So would the technique be the same for doing agar wedges? Clean box, utensils, close box, wipe down everything with iso. Wait 10 minutes and then get to work.
Have you ever personally done an agar to bag transfer. My concern is not being able to get a good seal on the bag, i figure a small incision could be made then taped. IDK anyone else?
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8ow8
Soylent McGriddle



Registered: 05/22/13
Posts: 196
Loc: Canada
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My record with bags is terrible... but if you're not averse to taking advice from somebody with a 30% contam rate (6 out of 20 bags! ugh!)...
Yup, it is fully possible to innoc a bag using agar in a still air box. I just drop the agar in the top of the bag, and then use the wire seal method. A heat sealer would be fine too.
-------------------- “There you go. Giving a fuck when it ain’t your turn to give a fuck.” My Trade list
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 3 months
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Re: Agar to MycoBAG? Possible? [Re: 8ow8]
#18992097 - 10/17/13 05:57 PM (10 years, 3 months ago) |
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Quote:
8ow8 said: My record with bags is terrible... but if you're not averse to taking advice from somebody with a 30% contam rate (6 out of 20 bags! ugh!)...
Yup, it is fully possible to innoc a bag using agar in a still air box. I just drop the agar in the top of the bag, and then use the wire seal method. A heat sealer would be fine too.
Yeah, I got these for free so I might as well use them right? And no I find no trouble in taking your advice since you are the only one who has responded to my thread, which to might I add, Thank you! I'm not a fan of bags either, well atleast so far I am not. I'm sure if I had an impulse sealer and a flowhood I would love bags! And why did you get so many contams? Is it from MS or agar transfers? And how did you go about doing it? I would love to try to do an agar to bag. I figured it would be just as easy as agar to grain jar. What do you think effects your results so drastically? Do you use a GB? What are your contam rates on jars? I'd say out of 20 jars, maybe one of mine will contaminate, but that it because I get lazy doing a bunch of jars in a cramped GB....
Thanks for any advice!
IS
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
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Of course you can inoculate filter patch bags of grains with an agar wedge. It's how I do it daily.
New growers would have a better success rate inoculating quart jars though. Once you get the quart jars of grains colonized contamination free, inoculate your large spawn bags via grain to grain transfers. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 3 months
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Re: Agar to MycoBAG? Possible? [Re: RogerRabbit]
#18992183 - 10/17/13 06:14 PM (10 years, 3 months ago) |
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Quote:
RogerRabbit said: Of course you can inoculate filter patch bags of grains with an agar wedge. It's how I do it daily.
New growers would have a better success rate inoculating quart jars though. Once you get the quart jars of grains colonized contamination free, inoculate your large spawn bags via grain to grain transfers. RR
I know that's how you do it. I have purchased every damn video and book you have ever published... Actually except that book about how mushrooms could be used to save the environment, I forgot the title at the moment. It is next on my list, next to Stephen Kings Doctor Sleep. I've been waiting 10 years to read that book.
But I digress, YOU MR.RR use a flowhood and most likely use an impulse sealer. I do not, so please break it down for me. How would I seal my bag? Just a zip tie? And the main problem was that I already shot up this bag with MS but I used that same MS syringe to knock up an agar dish, so I figure I got nothing to lose.. the grains are very sterile still. I went to out-grows meetup and it was wonderful, learned a great deal.
PS, I have some button mushrooms growing on cased grain, do you still recommend using a peat-moss casing layer?
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 3 months
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Re: Agar to MycoBAG? Possible? [Re: RogerRabbit]
#18992189 - 10/17/13 06:16 PM (10 years, 3 months ago) |
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Quote:
RogerRabbit said: New growers would have a better success rate inoculating quart jars though. Once you get the quart jars of grains colonized contamination free, inoculate your large spawn bags via grain to grain transfers. RR
I already stated I can do jars without any contam problems. I am being lazy and don't want to prep grains for one jar, especially since I have one perfectly sterile bag
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Heh, you just got confused with Stamets, go on RR lol.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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8ow8
Soylent McGriddle



Registered: 05/22/13
Posts: 196
Loc: Canada
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Quote:
nn-IlliniSpiralDMT said: And why did you get so many contams? Is it from MS or agar transfers?
No Idea. My guess is some combination of slightly high moisture content (my contams were all bacterial), and possible having used 5u rather than 2u filter patches - apparently some contams gan get through the 5u - but I'm not sure if this is speculation or proven. Everything was thoroughly PC'd, and the plates were carefully prepared, so the contam was likely introduced during or after inoculation. Conclusion: too much moisture, either sloppy glove box use or something got through the patches.
Quote:
nn-IlliniSpiralDMT said: And how did you go about doing it? I would love to try to do an agar to bag. I figured it would be just as easy as agar to grain jar. What do you think effects your results so drastically? Do you use a GB?
I use a large GB. I follow the standard tek: sterilize everything as it goes in the GB, spray the interior thoroughly with oust, wait 5 min, then get to work. I tried to minimize the amount of time each bag was open. The twist tie / wire approach is simple: Just introduce a plenum of air, twist shut the top of the bag (without losing the plenum), and then tightly wrap wire or a heavy duty twist tie around the twisted portion of the bag.
Quote:
nn-IlliniSpiralDMT said: What are your contam rates on jars? I'd say out of 20 jars, maybe one of mine will contaminate, but that it because I get lazy doing a bunch of jars in a cramped GB....
About the same. Last batch was 1/16, batch before that 0/16. Jars are easier to inoculate simply because they're smaller. I agree totally with RR above - it's easier to start with grain in a jar, and then transfer the colonized grain to a bag. That's what I'm doing in the next batch. because there is so much more myc in a GTG transfer, the colonization rates are much much higher - dramatically reducing opportunities for contams to take hold.
Good luck!
-------------------- “There you go. Giving a fuck when it ain’t your turn to give a fuck.” My Trade list
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 17 hours
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Re: Agar to MycoBAG? Possible? [Re: 8ow8]
#18994736 - 10/18/13 09:53 AM (10 years, 3 months ago) |
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Quote:
8ow8 said: I use a large GB. I follow the standard tek: sterilize everything as it goes in the GB
Impossible/unnecessary..... sanitized maybe.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (10/18/13 09:54 AM)
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 3 months
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Re: Agar to MycoBAG? Possible? [Re: maddchef]
#18994840 - 10/18/13 10:23 AM (10 years, 3 months ago) |
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Quote:
maddchef said: Heh, you just got confused with Stamets, go on RR lol.
Back in 08 Stamets was RR....maybe not anymore
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 17 hours
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Quote:
nn-IlliniSpiralDMT said:
Quote:
maddchef said: Heh, you just got confused with Stamets, go on RR lol.
Back in 08 Stamets was RR....maybe not anymore
Paul Stamets and Mark R. Keith were never the same person at anytime.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 3 months
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Re: Agar to MycoBAG? Possible? [Re: PussyFart]
#18994895 - 10/18/13 10:39 AM (10 years, 3 months ago) |
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Quote:
8ow8 said:
Quote:
nn-IlliniSpiralDMT said: And why did you get so many contams? Is it from MS or agar transfers?
No Idea. My guess is some combination of slightly high moisture content (my contams were all bacterial), and possible having used 5u rather than 2u filter patches - apparently some contams gan get through the 5u - but I'm not sure if this is speculation or proven. Everything was thoroughly PC'd, and the plates were carefully prepared, so the contam was likely introduced during or after inoculation. Conclusion: too much moisture, either sloppy glove box use or something got through the patches.
Quote:
nn-IlliniSpiralDMT said: And how did you go about doing it? I would love to try to do an agar to bag. I figured it would be just as easy as agar to grain jar. What do you think effects your results so drastically? Do you use a GB?
I use a large GB. I follow the standard tek: sterilize everything as it goes in the GB, spray the interior thoroughly with oust, wait 5 min, then get to work. I tried to minimize the amount of time each bag was open. The twist tie / wire approach is simple: Just introduce a plenum of air, twist shut the top of the bag (without losing the plenum), and then tightly wrap wire or a heavy duty twist tie around the twisted portion of the bag.
Quote:
nn-IlliniSpiralDMT said: What are your contam rates on jars? I'd say out of 20 jars, maybe one of mine will contaminate, but that it because I get lazy doing a bunch of jars in a cramped GB....
About the same. Last batch was 1/16, batch before that 0/16. Jars are easier to inoculate simply because they're smaller. I agree totally with RR above - it's easier to start with grain in a jar, and then transfer the colonized grain to a bag. That's what I'm doing in the next batch. because there is so much more myc in a GTG transfer, the colonization rates are much much higher - dramatically reducing opportunities for contams to take hold.
Good luck!
Quote:
Notahacker420 said:
Quote:
nn-IlliniSpiralDMT said:
Quote:
maddchef said: Heh, you just got confused with Stamets, go on RR lol.
Back in 08 Stamets was RR....maybe not anymore
Paul Stamets and Mark R. Keith were never the same person at anytime.
Damn do I feel I always had thought that Stamets was RR and that Roadkill was Mark R. Keith....
But whatever, my pscilly, thanks for clearing that up!
Anyway I'm just going to the transfer, I figure I have 5 dishes I can use and if it goes south I can just knock up a grain jar with some MS
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 3 months
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Notahacker420 are you from Illinois by any chance???
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 17 hours
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--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 3 months
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Re: Agar to MycoBAG? Possible? [Re: PussyFart]
#18995699 - 10/18/13 01:35 PM (10 years, 3 months ago) |
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Quote:
Notahacker420 said:

MY MAN
Country boy or city dweller? Have you gotten your concealed carry license yet?
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 17 hours
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Quote:
nn-IlliniSpiralDMT said: Country boy or city dweller? Have you gotten your concealed carry license yet?
I live in the city unfortunatley, and no one has their CCL yet.
They have just started classes, applications will be available in january...so the earliest will be march.
I am getting it.....you bet your ass....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (10/18/13 01:40 PM)
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 3 months
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Re: Agar to MycoBAG? Possible? [Re: PussyFart]
#18995812 - 10/18/13 02:04 PM (10 years, 3 months ago) |
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Quote:
Notahacker420 said:
Quote:
nn-IlliniSpiralDMT said: Country boy or city dweller? Have you gotten your concealed carry license yet?
I live in the city unfortunatley, and no one has their CCL yet.
They have just started classes, applications will be available in january...so the earliest will be march.
I am getting it.....you bet your ass....
Yeah me too, I have some cousins that are in LE and I should be taking some classes whenever they are available. Either way the price is going to be 200-350$ to take the stupid classes and waste 30+ hours of my time to instruct me on things I already know how to do
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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You two go flirt elsewhere lol
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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