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bakenast
Muffen Stuffer


Registered: 03/14/11
Posts: 920
Last seen: 1 year, 1 month
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agar recipe question
#18989965 - 10/17/13 09:08 AM (10 years, 4 months ago) |
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ive got plenty of agar ,but im out of LME that im use to using,, is there anything simple i can substitute for it so i dont have to order more... does brf/wbsf work? or maybe cornstarch? i think i have these already.. thanks
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: agar recipe question [Re: bakenast]
#18990310 - 10/17/13 10:44 AM (10 years, 4 months ago) |
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I wouldn't use BRF. Potatos can be cut up and simmered for their starch to make something like PDA(potoato dextrose agar) You can also use potato flakes. Some people get away with karo agar. Look at Frank's agar journal
agar recipes
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: agar recipe question [Re: bodhisatta]
#18991156 - 10/17/13 02:15 PM (10 years, 4 months ago) |
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Brown rice flour, or any grain flour, works quite well! I use grainwater agar for all agar- When none is handy I simply blend BRF thoroughly into water, let the sediment settle, and use the 'milky' water. This can result in nutrient-dilute agar if you don't use a good bit of BRF per water, so boiling the BRF into the water a bit can ensure richer solution (and perhaps shorten sterilization time by a few minutes if agar is added and sterilized right after boiling).
PDA (potato-dextrose agar) is the same thing, but requires another sugar (dextrose) since the potatoes aren't quite enough. This clearly isn't the case for grains.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
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Re: agar recipe question [Re: Violet]
#18991234 - 10/17/13 02:35 PM (10 years, 4 months ago) |
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Quote:
Violet said: Brown rice flour, or any grain flour, works quite well! I use grainwater agar for all agar- When none is handy I simply blend BRF thoroughly into water, let the sediment settle, and use the 'milky' water. This can result in nutrient-dilute agar if you don't use a good bit of BRF per water, so boiling the BRF into the water a bit can ensure richer solution (and perhaps shorten sterilization time by a few minutes if agar is added and sterilized right after boiling).
PDA (potato-dextrose agar) is the same thing, but requires another sugar (dextrose) since the potatoes aren't quite enough. This clearly isn't the case for grains.
Exactly what violet said, be careful though as you can make your agar TOO nutritious and cause stalled growth for your mycelium and increased growth for contaminates. Use 2 grams of BRF and boil in 500 ml of water, add 10 grams agar, and 1 gram karo.
I personally use 500ml water with 300 grams of potatos thrown in. I like to use a peeler to make the potato slices super thin. Boil that for 10 minutes. Strain out the chunks of potatos, add 9 grams agar, and 1 gram of karo.
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
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Brf agar
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
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Re: agar recipe question [Re: Stromrider]
#18991262 - 10/17/13 02:40 PM (10 years, 4 months ago) |
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Quote:
Stromrider said: Brf agar 
Don't you just love constructive criticism???
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
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Too nutritious
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: agar recipe question [Re: Stromrider]
#18991343 - 10/17/13 02:55 PM (10 years, 4 months ago) |
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No it's not, lol. For that matter, you control/effect the concentration, just like using 6,9,or 12g of malt extract in agar. So I don't see why it's logical to say that using a certain material ensures a too-nutritious mix.
Some people tell me grainwater agar is too nutritious, others tell me it's just dirty water, all of them think they represent the cutting edge of myco knowledge... where are the opinions from simple experience?
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 9 hours, 18 minutes
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Re: agar recipe question [Re: Violet]
#18991354 - 10/17/13 02:57 PM (10 years, 4 months ago) |
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I guess your right Just dilute it
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 4 months
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Re: agar recipe question [Re: Violet]
#18991401 - 10/17/13 03:10 PM (10 years, 4 months ago) |
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Quote:
Violet said: No it's not, lol. For that matter, you control/effect the concentration, just like using 6,9,or 12g of malt extract in agar. So I don't see why it's logical to say that using a certain material ensures a too-nutritious mix.
It isn't too nutritious, he obviously has not tried and it just trying to be SUPER GROWMAN. I made a agar recipe that RR said was too nutritious and at first nothing grew on the plates, no contams or mycelium. Then one week later the plates just exploded with growth. I added like 10 grams dog food, 12 grams agar, 750 ml water, 2.5 grams BRF, and some karo. SO no, i doubt just plain agar and BRF would be considered too nutritious.
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 9 hours, 18 minutes
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Hell yeah I am trying to be SUPER GROWMAN! Aren't we all?
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
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Re: agar recipe question [Re: Stromrider]
#18991469 - 10/17/13 03:23 PM (10 years, 4 months ago) |
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Quote:
Stromrider said: Hell yeah I am trying to be SUPER GROWMAN! Aren't we all?
What ever gets you hard amigo
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bakenast
Muffen Stuffer


Registered: 03/14/11
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Last seen: 1 year, 1 month
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good ideas, i was use to using 10g agar 9g LME without issues,, i forgot about being able to use dogfood.. thanks
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: agar recipe question [Re: bakenast]
#18993030 - 10/17/13 08:56 PM (10 years, 4 months ago) |
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Quote:
bakenast said: ive got plenty of agar ,but im out of LME that im use to using,, is there anything simple i can substitute for it so i dont have to order more... does brf/wbsf work? or maybe cornstarch? i think i have these already.. thanks
My agar recipe thread might provide some good ideas
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bakenast
Muffen Stuffer


Registered: 03/14/11
Posts: 920
Last seen: 1 year, 1 month
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ya, i read seen that thread are the list recipes youve tried? or were planning to try?? thanks
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bakenast
Muffen Stuffer


Registered: 03/14/11
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Last seen: 1 year, 1 month
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ya, i read seen that thread are the list recipes youve tried? or were planning to try?? thanks
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: agar recipe question [Re: bakenast]
#19010191 - 10/21/13 07:16 PM (10 years, 4 months ago) |
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relax, annie has ya covered on this.
sounds like ya need fert agar....
these pics should explain everything. hurry and gather these things, its cheap fast and easy.

youll need ferts, some grains/ or RGS, and some PP5 containers.once gathered, mix your fert water as directed. 10-10-10 is good.

once mixed, add the fert water to grain, and microwave boil. add agar. dont over do it, and dont forget to stir.

strain in a loose screen strainer, and bottle up!

works on many fungi , serves lots. germs spore fast, and encourages a good rhizo. full fruits easily, even with MS.

fert agar, annies choice for over 25 yrs. imagine fert agar and you..............
try it today, and, remember, " once ya fert, ya never revert"
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Edited by anne halonium (10/21/13 07:35 PM)
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bakenast
Muffen Stuffer


Registered: 03/14/11
Posts: 920
Last seen: 1 year, 1 month
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looks interesting, what the heck is fert? lol
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 9 hours, 18 minutes
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Re: agar recipe question [Re: bakenast]
#19012099 - 10/22/13 03:33 AM (10 years, 4 months ago) |
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Don't ask
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: agar recipe question [Re: Stromrider]
#19012627 - 10/22/13 08:55 AM (10 years, 4 months ago) |
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NPK fertilizer............available anywhere.
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