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OfflineDeadPhan
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Registered: 05/05/04
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Using spores from print on unaltered no pour jar lids for agar.
    #18985297 - 10/16/13 09:06 AM (10 years, 3 months ago)

so, i have been having a horrible run since august.  initially i believe i got a bad batch of syringes.  after innoculating straight on grain i had nothing but contams. 

i since put some solution from syringes onto agar and was able to clean up and isolate away from contams resulting in some finally clean jars after a long time.

in that amount of time, i also recieved a few spore prints from generous members on here due to my run of bad luck. 

however, in just over two weeks time, being from the oldest inoculation of spores from print, i havent had any germination yet. 

might one assume that the unaltered lids dont provide enough GE for spores from prints, likely being on the dryer side, to germinate in a shorter amount of time.  i keep the lids on very loose.  some of the prints seems not as dry, and hopefuly, those being ones i only moved onto agar about a week ago, will hopefully germinate.

just curious if i should maybe be making sure the spores get into the agar rather than just lying on top?  or if the unaltered lid will make it that much harder.  im going to be doing a bunch of innoculations today from print to agar and i plan on trying to make a divot where i will cool off the loop in the middle of the plate/jar and get the spores to land in there so as to maybe give them a chance of hydrateing better.  just wondering if anyone has an assumptions or suggestions. 
all of these prints are no older than early september.


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InvisibleSpitballJedi
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Registered: 10/13/12
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Re: Using spores from print on unaltered no pour jar lids for agar. [Re: DeadPhan]
    #18985429 - 10/16/13 10:01 AM (10 years, 3 months ago)

You don't need GE for spore germination. There should be plenty of air in the jar already. The GE hole is more to prevent a vacuum which might make it hard to get the lid off.

In RR's video on strain isolation, he uses an inoculation loop and kind of swirls the dry spores around on the agar.


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