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Offlinehihihi1717
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Accidentally inoc'd 3:1:1 pf jars (12 half pints)
    #18981203 - 10/15/13 12:47 PM (10 years, 4 months ago)

How fucked are the jars? Will they grow but at a slower rate? Everything else is perfect, I just fucked up my ratio. Can't find anything on the forum about at 3:1:1 ratio. In any case, I'll let them sit and let you guys know if anything grows as time goes on. Just want to know how bad I fucked myself according to modern mycology standards in regardsto the pf tek.


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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: hihihi1717]
    #18981209 - 10/15/13 12:51 PM (10 years, 4 months ago)

You fucked up good. Just start over BRF and verm are cheap as hell. The jars are going to be way to dry with 3verm 1brf 1h2o


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Offlinehihihi1717
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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: bodhisatta]
    #18981218 - 10/15/13 12:53 PM (10 years, 4 months ago)

There's no hope at all for any growth?


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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: hihihi1717]
    #18981225 - 10/15/13 12:55 PM (10 years, 4 months ago)

I guess I would try it to see what happened if I had 12 other jars I could prepare the right way. If these are your only jars I have a feeling you'll just set yourself back a week


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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: bodhisatta]
    #18981230 - 10/15/13 12:56 PM (10 years, 4 months ago)

BTW how the hell did that happen. You should be testing for field capacity and adjusting the water content as needed by adding h2o or adding verm to make it just right. That mix would have obviously been off and you could have just added more water and BRF before loading your jars.


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Offlinehihihi1717
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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: bodhisatta]
    #18981238 - 10/15/13 12:58 PM (10 years, 4 months ago)

I'll order a new spore syringe and wait these jars out for a couple weeks. The substrate doesn't look dry at all, though, surprisingly. It looks very much like the substrate seen in the tek vid at mushroomvideos.com. Obviously mine is going to be different though. Hopefully I can get some pics up for a better look.


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Offlinehihihi1717
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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: hihihi1717]
    #18981245 - 10/15/13 01:00 PM (10 years, 4 months ago)

I fucked up the ratio by using outdated info thats says to use 3/4 cup verm, 1/4 cup brf and water and didn't realize this was a 3:1:1 ratio as opposed to 2:1:1 which I was in fact aiming for. So it's mostly just overlooking an information discrepency.


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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: hihihi1717]
    #18981262 - 10/15/13 01:05 PM (10 years, 4 months ago)

Where did you find that outdated info. Maybe the 3-1-1 works but sub optimal? you can do what you want with the jars though


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Offlinehihihi1717
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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: bodhisatta]
    #18981290 - 10/15/13 01:12 PM (10 years, 4 months ago)

I don't have a link readily available (on mobile), but it's the 490k + view vid on Youtube called How To Grow Magic Mushrooms, I believe. By my estimates, the video is at least 15-20 years old. They do a lot of shit in that video that would give our more experienced mycologists an aneurism if they watched someone doing it. They recommend 87 degrees Fahrenheit for a colonization temperature, for instance. If you're ever bored and want a few lulz, you might enjoy watching it, hehe.


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Offlinehihihi1717
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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: hihihi1717]
    #18981510 - 10/15/13 02:07 PM (10 years, 4 months ago)





Those are 3 of my 12 jars.

The Youtube video I mentioned can be found here:



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OfflineHyphaeKnoT
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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: hihihi1717]
    #18981825 - 10/15/13 03:31 PM (10 years, 4 months ago)

Yeah -- it's 1 cup verm., 1/2 cup BRF, and 1/2 cup distilled water.  Good luck mate!!


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OfflineMush4Brains
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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: HyphaeKnoT]
    #18981898 - 10/15/13 03:46 PM (10 years, 4 months ago)

Quote:

HyphaeKnoT said:
Yeah -- it's 1 cup verm., 1/2 cup BRF, and 1/2 cup distilled water.  Good luck mate!!





It is a 2:1:1 ratio.  It has nothing to do with cups, it could be gallons, or liters, or quarts, or pints.  You also don't use distilled water.


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OfflineGiggle_Grower
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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: Mush4Brains]
    #18981944 - 10/15/13 03:57 PM (10 years, 4 months ago)

I use spring or rain water. And you want the widemouth halfpint jars. Those reg mouth jars tend to stall fequently at around the bottom. If you cant find them in the canning section of a store(i never have, but others occasionally do) then you can order them from any ACE hardware store. They are the same price and usually take a week to arrive IME.


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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: Giggle_Grower]
    #18981957 - 10/15/13 04:01 PM (10 years, 4 months ago)

And fill those jars up with dry verm so it cant move around! :smile:


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Offlinehihihi1717
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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: Pestile]
    #18983914 - 10/15/13 11:04 PM (10 years, 4 months ago)

That actually is dry verm at the top, but for whatever reason it turns that way after we PC the jars. We even used micropore tape with the tin foil, but I don't know. What's weird is it looks like that with a 3:1:1 ratio. You'd think it would look like that if it were a higher amount of water. Perhaps the use of distilled water can do this?

Anyways, one of the jars has what is either cobweb mold or mycelium starting to colonize, but the spot where I see it is so small it is impossible to tell. It appears rhysomorphic (like a tiny, tiny area at the inoculation point where white strands appear to be stretching out), so I'm optimistic about jar #2. We'll see what happens in the coming days, though!

I guess assuming whatever jars that do colonize don't become contaminated I'll have at least some success. And then I can get another batch going in the mean time. If I have to, I'll settle for one or two jars this time around :shrug:


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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: hihihi1717]
    #18984024 - 10/15/13 11:25 PM (10 years, 4 months ago)

you should be okay its just a little less water then normal... just hope they dont stall due to dehydration. u also have less brf in there so u do have a little bit of more water going to verm.


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Offlinehihihi1717
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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: silverstem]
    #18984102 - 10/15/13 11:42 PM (10 years, 4 months ago)

Is there anything one can do to hydrate pf cakes while still colonizing? I'm guessing not but it's worth a shot if there's a way.


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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: hihihi1717]
    #18984126 - 10/15/13 11:48 PM (10 years, 4 months ago)

i wouldnt... you could run the risk of having pooling water or u could shoot up mold microspores into it... keep them as is.


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Offlinehihihi1717
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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: silverstem]
    #18984685 - 10/16/13 02:49 AM (10 years, 4 months ago)

Sounds good. I'll update this thread as I see progress. Hopefully I can report back on jar #2 and others soon.


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OfflineGiggle_Grower
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Re: Accidentally inoc'd 3:1:1 pf jars (12 half pints) [Re: hihihi1717]
    #18984761 - 10/16/13 03:56 AM (10 years, 4 months ago)

Agreed. Ride it out. And get the proper jars! Lol


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