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OfflineNeutronova
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Registered: 09/05/13
Posts: 5
Last seen: 9 years, 10 months
Newbie here with a contam question.
    #18983336 - 10/15/13 08:44 PM (10 years, 3 months ago)

As the title says im new to growing and have hit an utter brick wall in progress and am running out of patience.

I use the RR PF tek. I have tried steaming, but in hopes my problem was improper jar sterilization I bought a PC but I continue to run into the same problem.

As far as I can tell I follow the tek strictly, but after a series of jar inoculations (5 at a time) I have gotten trich eventually in every single jar (25 total) before any cake as come even close to consolidating (closest jar before contam was at maybe 70%). I keep a grow journal, which isn't very long cause I cant even get to the birthing phase of a grow.

Is it possible to buy contaminated LC? I have bought 2 syringes both from the same location and every single jar has failed despite my best attempts to work in as sterile of an environment as possible.

During my search on the forums the only thing I could find was basically RR saying.

"If your jar had green mold when you birthed it, either your inoculation procedure was poor, or your filter didn't do its job. If the green was right at the top by the verm barrier(assuming pf tek) it got in that way. If it grew from an inoculation point, it got in that way."

Is this the hard and fast rule when it comes to trich? and is there no other possibility other then I keep fucking up my inoculations?


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OfflineStupendous-Yappi
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Re: Newbie here with a contam question. [Re: Neutronova]
    #18983948 - 10/15/13 11:10 PM (10 years, 3 months ago)

Quote:

Neutronova said:
During my search on the forums the only thing I could find was basically RR saying.

"If your jar had green mold when you birthed it, either your inoculation procedure was poor, or your filter didn't do its job. If the green was right at the top by the verm barrier(assuming pf tek) it got in that way. If it grew from an inoculation point, it got in that way."

Is this the hard and fast rule when it comes to trich? and is there no other possibility other then I keep fucking up my inoculations?



That should apply to any contaminate, if the jars were truly sterilized. Those are the only two way's for something to get in.

Does the trich start at the verm barrier?


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Invisible8ow8
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Re: Newbie here with a contam question. [Re: Neutronova]
    #18985420 - 10/16/13 09:58 AM (10 years, 3 months ago)

Quote:

Neutronova said:
Is it possible to buy contaminated LC? I have bought 2 syringes both from the same location and every single jar has failed despite my best attempts to work in as sterile of an environment as possible.






Definitely possible. I've seen syringes with total contam cornucopias. When I use a new syringe, I always start by putting a drop in the center of 2 or 3 petri dishes. That will make it possible to see if the syringe is contaminated, and if so, to isolate mycelium and clear the contaminants. In my experience LC simply sucks because it is too prone to invisible contams that show up once you've inoculated. If you can't get spores from another source, I'd very strongly recommend getting started on agar.

Syringe or spores >> agar >> agar isolate >> grain


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OfflineKizzle
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Re: Newbie here with a contam question. [Re: 8ow8]
    #18985823 - 10/16/13 11:56 AM (10 years, 3 months ago)

Quote:

Is it possible to buy contaminated LC? I have bought 2 syringes both from the same location and every single jar has failed despite my best attempts to work in as sterile of an environment as possible.



Yes. I'd say that's very likely all things considered - 100% contamination rate, all green mold, especially if you're making your own LCs from spores.


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OfflineNeutronova
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Registered: 09/05/13
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Last seen: 9 years, 10 months
Re: Newbie here with a contam question. [Re: Kizzle]
    #18987752 - 10/16/13 07:14 PM (10 years, 3 months ago)

Thank you for the replies.

I will start researching Agar, I hear it is more complicated a procedure but every post I have came across all say its the way to go but its more advanced. To be on honest as I sit here typing this I have literally no clue what it is. but i'm sure since it seems so popular there will be easy to find posts on the matter in these forums.

Once again, thanks for helping out a newbie, any further general advice someone might have will be greatly appreciated as I scrap the LC approach and figure out what agar is :smile:


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OfflineMAIA
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Re: Newbie here with a contam question. [Re: Neutronova]
    #18987961 - 10/16/13 08:04 PM (10 years, 3 months ago)

Yeah, go with agar. Agar is a solid medium on which you can grow mycelium, either from spores, from mycelium itself or from sections of the fruitbody. There are plenty of recipes and tek's. Good luck with your demand for knowledge :smile:


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OfflineBoudicca
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Registered: 09/22/13
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Loc: Ontario
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Re: Newbie here with a contam question. [Re: MAIA]
    #18998721 - 10/19/13 07:49 AM (10 years, 3 months ago)

Sorry you're running into such trouble. My first grow was blessedly uneventful, but I've been having problems with trich for my second grow and it's very frustrating.

I think someone asked if your trich was showing up at the verm barrier. This is very important, if you follow RR's tek, they tell you to make sure to carefully wipe down the top inch or so where your verm barrier will be with an alcohol pad, and to make sure this area is completely free of the BRF mixture. I had inadvertently missed this step for one batch and ended up getting trich a few times starting at the barrier.

As others have suggested, perhaps try to obtain spore syringes from another supplier to see if you are having the same difficulty.

Good luck!


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OfflineNeutronova
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Registered: 09/05/13
Posts: 5
Last seen: 9 years, 10 months
Re: Newbie here with a contam question. [Re: Boudicca]
    #19004552 - 10/20/13 04:00 PM (10 years, 3 months ago)

Thank for the advice, I do wipe down the lips of the jars before putting in the top layer of verm. However when I opened the jars I noticed that top layer was soaked. I;m not sure if that's not normal, of if it means water is getting in during the sterilization, I tape the holes so I don't see how that's possible. But as far as the origin of the trich i'm pretty sure they where all at the inoculation points, which is why I was wondering if LC can be contaminated, because i'm pretty good (I think) with sterile inoculations. I'm going to get a spore syringe from a different source and see if that makes a difference and if it doens't then my methods are still the issue. Trouble shooting this stuff takes so much time and patience.

I'm happy to hear your first grow was incident free :smile:


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