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Offlineaomoriakuma
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Registered: 09/09/13
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any LC teks you recommend?
    #18969563 - 10/12/13 07:16 PM (10 years, 4 months ago)

i know most of you dont recommend LCs, but im doing it for the experience and because i find it interesting, so dont try to talk me out of it!

anyway, so i just noc'd up 5 master jars, 2 GT and 3 Z+. i have about 2cc's of spore solution left of each and i thought, what better to do with them than turn it into more! So i figure ill try my hand at a liquid culture while i wait for my master jars to finish up (:

i looked at a few teks, but most seem outdated. from what i read, light malt extract works better than karo, and jars better than baby bottles etc. so i just wanted to see if anyone knows of a good tek that might help me do this right!

thanks!


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OnlineSupaThaRipper
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Re: any LC teks you recommend? [Re: aomoriakuma]
    #18969589 - 10/12/13 07:25 PM (10 years, 4 months ago)

I highly recommend getting a stir plate to maximize growth.

300ml water

1 tbsp light karo syrup.

Pressure cook for 30min @ 60psi


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OfflineOgreLokon
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Re: any LC teks you recommend? [Re: SupaThaRipper]
    #18969633 - 10/12/13 07:42 PM (10 years, 4 months ago)

Quote:

SupaThaRipper said:
Pressure cook for 30min @ 60psi




Do PCs go that high?


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Offlineitsaconspiracy
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Re: any LC teks you recommend? [Re: OgreLokon]
    #18969657 - 10/12/13 07:49 PM (10 years, 4 months ago)

no I'm pretty sure he means 15psi but 30mins sounds to long i think 20 would be plenty.


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Offlineaomoriakuma
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Re: any LC teks you recommend? [Re: itsaconspiracy]
    #18970300 - 10/12/13 10:45 PM (10 years, 4 months ago)

what exactly is a stir plate?

im assuming its something to agitate the myc growth..


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Offlinetwistedty
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Re: any LC teks you recommend? [Re: aomoriakuma]
    #18970425 - 10/12/13 11:18 PM (10 years, 4 months ago)

agars easier and you dont need stirrers :twocents:


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InvisiblePastywhyteMDiscord
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Re: any LC teks you recommend? [Re: twistedty]
    #18970550 - 10/12/13 11:50 PM (10 years, 4 months ago)

Quote:

twistedty said:
agars easier and you dont need stirrers :twocents:




:whathesaid: QFT

IMO LC does have a purpose in mycology for growing species like mexicana, which don't really like a shake. To speed my experiments with those up, I am planning to try and get some LC going right away. However that being said, I'd be more concerned with what I was using to inoculate the LC, rather than what kind of LC it was. From what I have read malt, karo, or SITR all seem to have a similar success rate provided the LC is clean. On the other hand the only inoculate I would trust for LC would have to be an agar wedge.

So if your growing cubes I would not bother with an LC period. Being able to shake cubes pretty much negates any benefit LC may have over just inoculating with a wedge, but LC does introduce a lot of drawbacks :shrug:

PS, you only need a drop of spore solution to inoculate an agar plate. 2cc's could do many many plates :awesomenod:


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Invisiblewildernessjunkie
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Re: any LC teks you recommend? [Re: Pastywhyte]
    #18970711 - 10/13/13 12:37 AM (10 years, 4 months ago)

Quote:

On the other hand the only inoculate I would trust for LC would have to be an agar wedge.




Agreed. Anything less is a gamble with contamination. My opinion is that if you cant do agar, you shouldn't do LC.

Though properly used, and verified clean LC can be an extremely powerful tool.


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OnlineSupaThaRipper
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Re: any LC teks you recommend? [Re: wildernessjunkie]
    #18971300 - 10/13/13 07:35 AM (10 years, 4 months ago)

Lmao 15 whoops


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Offlineaomoriakuma
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Re: any LC teks you recommend? [Re: SupaThaRipper]
    #18978984 - 10/14/13 09:59 PM (10 years, 4 months ago)

hmm, yeah i dont know why ive been so hesitant to try an agar wedge lol. ive heard nothing but good things, yet i somehow look right past it anytime i see mention of it.

ive been busy and havent gotten around to do the LC, so maybe ill go with a wedge instead. seems like the general consensus is that wedges are the way to go :p


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Offlinepartythug
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Re: any LC teks you recommend? [Re: aomoriakuma]
    #18981178 - 10/15/13 12:36 PM (10 years, 4 months ago)

If I'm doing any LC, I'll stick to a Grain Liquid Culture. Easy, fool-proof, and better for avoiding contamination, in my experience.

Use the search function for details. Nutshell: use one of those jars when fully colonized, or make a smaller jar just for a 60cc syringe or two. If you've got the right lids, properly sterilized water, and competent sterile technique, you'll do just fine. No need to mess with sugary liquids.


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Offlinemason man
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Re: any LC teks you recommend? [Re: aomoriakuma]
    #18981329 - 10/15/13 01:22 PM (10 years, 4 months ago)

Quote:

aomoriakuma said:
i know most of you dont recommend LCs




Is this true? I just started some today... I'm doing it for the purpose of having a lot of inoculate available, and I can get jars ready in shorter time.

I mean, LC just seems more convenient... plus, for a beginner, isn't opening a jar and putting a wedge in a technique that potentially has a higher chance of contam? Meaning you're sterile technique has to be pretty flawless.


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OfflinePussyFart
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Re: any LC teks you recommend? [Re: mason man]
    #18981351 - 10/15/13 01:29 PM (10 years, 4 months ago)

Quote:

mason man said:
Quote:

aomoriakuma said:
i know most of you dont recommend LCs




Is this true? I just started some today... I'm doing it for the purpose of having a lot of inoculate available, and I can get jars ready in shorter time.



I hate it when people think like this....lol.

Just inoculate the cakes or whatever with the spores.

You will have to wait for them to germinate reguardless, then you will have to wait for the LC to grow, shoot up test jars and grow them out, then finally inoculate your jars if you find that your LC is clean.

That is all fine and well, but your jars would be nearing completion by the time the LC is ready to be tested had you just used the spores in the first place.

LC adds nothing but time, risk, and more work IMO.

No spore print/syringe is ever 100% clean, and LC is the perfect breeding ground for anything nasty.

Unless you are starting the LC from a clean agar wedge, just skip it.

And once you are doing agar, you will most likely not want to mess with LCs....it's kinda funny how that works.
Quote:

mason man said:
Quote:

aomoriakuma said:
I mean, LC just seems more convenient... plus, for a beginner, isn't opening a jar and putting a wedge in a technique that potentially has a higher chance of contam? Meaning you're sterile technique has to be pretty flawless.



No, if done in a still air box or in front of a laminar flow hood making a transfer is way less risky than making an LC...in my opinion anyways.

The jar is only open for a second or 2, in a clean still environment, or in front of a sterile air flow.


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Offlinemason man
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Re: any LC teks you recommend? [Re: PussyFart]
    #18981374 - 10/15/13 01:37 PM (10 years, 4 months ago)

Quote:

Notahacker420 said:
That is all fine and well, but your jars would be nearing completion by the time the LC is ready to be tested had you just used the spores in the first place.




Guess I got bored while I'm waiting for my current jars to colonize and needed something new to try :smile:

Quote:

No spore print/syringe is ever 100% clean, and LC is the perfect breeding ground for anything nasty.




So will contams not be easily visible in a LC as they are in agar? If my LC ends up looking clean, does that mean it isn't necessarily?


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OfflinePussyFart
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Re: any LC teks you recommend? [Re: mason man]
    #18981401 - 10/15/13 01:41 PM (10 years, 4 months ago)

Quote:

mason man said:
So will contams not be easily visible in a LC as they are in agar? If my LC ends up looking clean, does that mean it isn't necessarily?



There is no way to visually confirm if an LC is clean or not, test jars must be shot up and grown out. Also there is no way to transfer away from a contaminated LC, you just toss the whole thing.

Contams on agar are obvious as day, and can usually be transferred away from very easily.


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Offlinemason man
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Re: any LC teks you recommend? [Re: PussyFart]
    #18981532 - 10/15/13 02:14 PM (10 years, 4 months ago)

Quote:

Notahacker420 said:
Quote:

mason man said:
So will contams not be easily visible in a LC as they are in agar? If my LC ends up looking clean, does that mean it isn't necessarily?



There is no way to visually confirm if an LC is clean or not, test jars must be shot up and grown out. Also there is no way to transfer away from a contaminated LC, you just toss the whole thing.

Contams on agar are obvious as day, and can usually be transferred away from very easily.




Okay, I know I've kind of hijacked this thread, but its all on-topic.

When using agar, is it always best to find a fast growing isolate? I've seen on here multiple times that isolates can be a crap shoot as well without testing a bunch of them to finding one which fruits.

Hacker, your "tale of 10 isolates" kind of confirms this as well. So if I'm not going for an isolate for fear of it being a non-fruiting strain, can I still use agar just for the sake of finding clean myc? Would I just take a sector with multiple strains in it to assure a more wide range of genetics?


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OfflinePussyFart
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Re: any LC teks you recommend? [Re: mason man]
    #18981802 - 10/15/13 03:26 PM (10 years, 4 months ago)

Quote:

mason man said:
When using agar, is it always best to find a fast growing isolate?



That's what I like to do...

Quote:

mason man said:
I've seen on here multiple times that isolates can be a crap shoot as well without testing a bunch of them to finding one which fruits.



This is true....this is why I like to take a sample from a fruit and isolate that...it's kind of a shortcut, and the cultures are almost guaranteed to fruit.

Quote:

mason man said:
Hacker, your "tale of 10 isolates" kind of confirms this as well. So if I'm not going for an isolate for fear of it being a non-fruiting strain, can I still use agar just for the sake of finding clean myc? Would I just take a sector with multiple strains in it to assure a more wide range of genetics?



Well, after a long and hard personal debate...I came to the conclusion that there might have been a lot of hidden contams in the substrate because I used the bucket tek vs proper pasteurization.

I am currently running 10 tubs of each isolate, slowly but surely, using proper pasteurization to confirm whether I got a bunch of non fruiting isolates from clones or did the hidden contams that I might have had hinder the substrate from fruiting.

It could go either way....only time will tell....I will update that post if and when I find anything out.


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Offlineaomoriakuma
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Re: any LC teks you recommend? [Re: PussyFart]
    #18982782 - 10/15/13 07:16 PM (10 years, 4 months ago)

ive been hijacked!

lol anyone have an agar tek they recommend? since im leaning in that direction now


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InvisiblebodhisattaMDiscordReddit
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Re: any LC teks you recommend? [Re: aomoriakuma]
    #18982890 - 10/15/13 07:29 PM (10 years, 4 months ago)

Quote:

aomoriakuma said:
ive been hijacked!

lol anyone have an agar tek they recommend? since im leaning in that direction now




It's the best direction you can lean in.


The obvious one

Frank's recipes


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Offlinetwistedty
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Re: any LC teks you recommend? [Re: bodhisatta]
    #18987762 - 10/16/13 07:17 PM (10 years, 4 months ago)

LCs :/.

i know there are alot of agar recipes out there, but i use just PDA from mycosupply its just add water pc and pour dishes.

ive done LCs for fun to see what everyone is talking about and man is it a PITA i had success with it but there is so many extra steps and i made a magnet stirrer and all other stupid shit its dumb.

i used clean wedge in lc not ms and ive said this 100x but id rather drop wedge in grain jar and then g2g. also you have to do ports in your lc jar sterile syringes and the list goes on of all kinds of other pita steps.

agar is just as easy to make as jello, just need a pc and any kind of glass vessel


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