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JahLambsbread
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Registered: 08/08/10
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Last seen: 6 years, 4 months
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agar prep
#18981044 - 10/15/13 11:55 AM (10 years, 4 months ago) |
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ok i have oatmeal and one tek for agar says 40 grams of oatmeal to nine grams agar agar to a certain amount of distilled water. It also says to use Neopeptone but idk what that is.
This is kind of simple, should I ad dextrose because I have some. OR do you think I should go get potatoe flakes?
I also have yeast flakes
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Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
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do yourself a favor. Go get a bag of ALL NATURAL dry dog food. Make sure it says all natural on the bag. the brand doesnt matter. pulverize it and mix it with equal parts agar by weight. 10 grams dog food and 10 grams agar in 500 ml of water will pour a sleeve of 25 plates. best agar you can make for the money. ALL NATURAL!! look for lassie brand or nutri science brand.
Here is a comparison of DFA(DOG FOOD AGAR) and PDYA (POTATO DEXTROSE YEAST AGAR) same strain of mushroom which one looks better to ya?
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Don't use distilled water for anything in this hobby except to fill up a humidifier.
Frank has an agar journal Here I found it for you AGAR!!
Otherwise use 10g nutrient 10g agar 500ml h2o to start I use extra-light malt extract you can use ELME or LME Dog food works if it's all natural, the water you soak your grain in too. potato flakes etc..
Edited by Trusted cuItivator (10/15/13 12:54 PM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Quote:
bodhisatta said: Don't use distilled water for anything in this hobby except for making spore syringes.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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JahLambsbread
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Registered: 08/08/10
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I just wasted 500 ml of distilled water damn.
I pressure cooked it and it was still liquid. I there something I did wrong besides distilled water? I put 10 grams dog food and ten grams agar to 500 ml water. the water seemed like WAY too much
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
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It is going to be liquid until it cools to room temperature.
You want to pour your dishes right before it solidifies, so there is less condensation that forms.
Your agar will still be fine if you used distilled, it's just not the best you could have chosen...it still works just fine.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Quote:
Notahacker420 said:
Quote:
bodhisatta said: Don't use distilled water for anything in this hobby except for making spore syringes.

True that thanks for fixing.
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abltsandwich
JFK = Jelly Donut




Registered: 06/16/09
Posts: 11,537
Loc: Dildoville
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Distilled water does well in liquid cultures for clarity but that's off topic don't want to derail.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
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Quote:
abltsandwich said: Distilled water does well in liquid cultures for clarity but that's off topic don't want to derail.
Distilled water will leach minerals out of whatever it touches.
It is not good for anything except spore syringes....not even LCs...Tap water would perform slightly better.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 7 months, 11 days
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Quote:
Notahacker420 said:
Quote:
abltsandwich said: Distilled water does well in liquid cultures for clarity but that's off topic don't want to derail.
Distilled water will leach minerals out of whatever it touches.
It is not good for anything except spore syringes....not even LCs...Tap water would perform slightly better.
Distiller water is fine for agar. It doesn't matter because there is so much solute in the agar. In just goes into solution. Distiller water does leach nutrients out of LIVING cells though. That why ya shouldn't drink it constantly.
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JahLambsbread
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Registered: 08/08/10
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Last seen: 6 years, 4 months
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Ok can I use potato flakes with butter and garlic in them to mix with agar?
I also have fries from wendy's. Would that work??
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Quote:
JahLambsbread said: Ok can I use potato flakes with butter and garlic in them to mix with agar?
I also have fries from wendy's. Would that work??
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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