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OfflineJahLambsbread
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agar prep
    #18981044 - 10/15/13 11:55 AM (10 years, 4 months ago)

ok i have oatmeal and one tek for agar says 40 grams of oatmeal to nine grams agar agar to a certain amount of distilled water. It also says to use Neopeptone but idk what that is.

This is kind of simple, should I ad dextrose because I have some. OR do you think I should go get potatoe flakes?

I also have yeast flakes


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OfflineAmanita virosa
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Re: agar prep [Re: JahLambsbread]
    #18981131 - 10/15/13 12:19 PM (10 years, 4 months ago)

do yourself a favor.  Go get a bag of ALL NATURAL dry dog food.  Make sure it says all natural on the bag.  the brand doesnt matter. pulverize it and mix it with equal parts agar by weight.  10 grams dog food and 10 grams agar in 500 ml of water will pour a sleeve of 25 plates.  best agar you can make for the money.  ALL NATURAL!! look for lassie brand or nutri science brand.

Here is a comparison of DFA(DOG FOOD AGAR) and PDYA (POTATO DEXTROSE YEAST AGAR)  same strain of mushroom  which one looks better to ya?


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InvisiblebodhisattaMDiscordReddit
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Re: agar prep [Re: Amanita virosa]
    #18981219 - 10/15/13 12:53 PM (10 years, 4 months ago)

Don't use distilled water for anything in this hobby except to fill up a humidifier.

Frank has an agar journal
Here I found it for you
AGAR!!

Otherwise use 10g nutrient 10g agar 500ml h2o to start
I use extra-light malt extract you can use ELME or LME
Dog food works if it's all natural, the water you soak your grain in too. potato flakes etc..


Edited by Trusted cuItivator (10/15/13 12:54 PM)


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OfflinePussyFart
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Re: agar prep [Re: bodhisatta]
    #18981407 - 10/15/13 01:43 PM (10 years, 4 months ago)

Quote:

bodhisatta said:
Don't use distilled water for anything in this hobby except for making spore syringes.



:thumbup:


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OfflineJahLambsbread
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Re: agar prep [Re: PussyFart]
    #18981656 - 10/15/13 02:50 PM (10 years, 4 months ago)

I just wasted 500 ml of distilled water damn.

I pressure cooked it and it was still liquid.  I there something I did wrong besides distilled water?  I put 10 grams dog food and ten grams agar to 500 ml water.  the water seemed like WAY too much


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OfflinePussyFart
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Re: agar prep [Re: JahLambsbread]
    #18981828 - 10/15/13 03:32 PM (10 years, 4 months ago)

It is going to be liquid until it cools to room temperature.

You want to pour your dishes right before it solidifies, so there is less condensation that forms.

Your agar will still be fine if you used distilled, it's just not the best you could have chosen...it still works just fine.


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InvisiblebodhisattaMDiscordReddit
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Re: agar prep [Re: PussyFart]
    #18982310 - 10/15/13 05:26 PM (10 years, 4 months ago)

Quote:

Notahacker420 said:
Quote:

bodhisatta said:
Don't use distilled water for anything in this hobby except for making spore syringes.



:thumbup:




True that thanks for fixing.


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Invisibleabltsandwich
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Re: agar prep [Re: bodhisatta]
    #18982325 - 10/15/13 05:30 PM (10 years, 4 months ago)

Distilled water does well in liquid cultures for clarity but that's off topic don't want to derail.


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OfflinePussyFart
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Re: agar prep [Re: abltsandwich]
    #18982357 - 10/15/13 05:38 PM (10 years, 4 months ago)

Quote:

abltsandwich said:
Distilled water does well in liquid cultures for clarity but that's off topic don't want to derail.



Distilled water will leach minerals out of whatever it touches.

It is not good for anything except spore syringes....not even LCs...Tap water would perform slightly better.


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OfflineAmanita virosa
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Re: agar prep [Re: PussyFart]
    #18982639 - 10/15/13 06:47 PM (10 years, 4 months ago)

Quote:

Notahacker420 said:
Quote:

abltsandwich said:
Distilled water does well in liquid cultures for clarity but that's off topic don't want to derail.



Distilled water will leach minerals out of whatever it touches.

It is not good for anything except spore syringes....not even LCs...Tap water would perform slightly better.



Distiller water is fine for agar. It doesn't matter because there is so much solute in the agar. In just goes into solution. Distiller water does leach nutrients out of LIVING cells though. That why ya shouldn't drink it constantly.


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OfflineJahLambsbread
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Re: agar prep [Re: Amanita virosa]
    #18990481 - 10/17/13 11:19 AM (10 years, 4 months ago)

Ok can I use potato flakes with butter and garlic in them to mix with agar? 

I also have fries from wendy's. Would that work??


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OfflinePussyFart
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Re: agar prep [Re: JahLambsbread]
    #18990485 - 10/17/13 11:20 AM (10 years, 4 months ago)

Quote:

JahLambsbread said:
Ok can I use potato flakes with butter and garlic in them to mix with agar? 

I also have fries from wendy's. Would that work??



:asianofapproval:


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