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OfflineJahLambsbread
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How to rehydrate tissue that has dried?
    #18982283 - 10/15/13 05:18 PM (10 years, 3 months ago)

I am only posting so much because every time I ask a question no one answers and instead just talk about something completely different.



How do you rehydrate a mushroom tissue that has been dried? Someone told me to let it dry and rehydrate it.


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OfflineJahLambsbread
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Re: How to rehydrate tissue that has dried? [Re: JahLambsbread] * 1
    #18982286 - 10/15/13 05:19 PM (10 years, 3 months ago)

and it's for cloning in agar


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InvisibleSpitballJedi
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Re: How to rehydrate tissue that has dried? [Re: JahLambsbread]
    #18982384 - 10/15/13 05:45 PM (10 years, 3 months ago)

You can make your agar a little wetter than usual, but basically, just put a small sample on agar.

Be prepared to isolate it from contamination. There will be contamination.

As soon as you see myc growth, you need to isolate it asap, before contamination can over run it.

Some may suggest using a less nutritive agar recipe. This can produce more distinguished rhyzomorphic growth.

I am not speaking from experience, but from logic and what I've read on the subject. I have not come across anyone hydrating the dry specimen before they put it on agar.

Search "tissue revival"


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Edited by SpitballJedi (10/15/13 05:49 PM)


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InvisibleFooMan
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Re: How to rehydrate tissue that has dried? [Re: JahLambsbread]
    #18982431 - 10/15/13 05:56 PM (10 years, 3 months ago)

Drop the tissue in some sterilized distilled water until it looks hydrated. It will eventually start growing in the water if aerated if theres a chance for it. Then drop it on your plate. You'll need to isolate away from contaminants, assuming the tissue starts growing. Good luck!





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Quick WBS Prep


Edited by FooMan (10/15/13 06:08 PM)


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InvisibleSpitballJedi
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Re: How to rehydrate tissue that has dried? [Re: SpitballJedi]
    #18982630 - 10/15/13 06:45 PM (10 years, 3 months ago)

Quote:

SpitballJedi said:
I have not come across anyone hydrating the dry specimen before they put it on agar.




I can't say that anymore :thumbup:


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