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sytar
Radiant


Registered: 09/01/13
Posts: 381
Last seen: 9 years, 5 months
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Re: first grow 12 days after inoc [Re: N2ocean]
#18988378 - 10/16/13 09:27 PM (10 years, 3 months ago) |
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From what I understand from reading RR people keep their jars too warm because of that experiment showing best mycelial growth at 86 degrees. But that experiment used agar plates and the inside of the jars generated heat so people dropped the temp down to around 80-82 to capitalize on these optimum grow temperatures. If I recall correctly RR did some experiments using jars and found the jars started growing quite a bit slower around 82 degrees. Seems like more recent recommendations for jar temps tend to be around the mid 70s. Risk of contams also goes up a lot around 80 from what I understand.
-------------------- I post from my phone. Excuse the typos and autocorrects.
Edited by sytar (10/16/13 09:30 PM)
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 18 hours, 28 minutes
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Re: first grow 12 days after inoc [Re: sytar]
#18988646 - 10/16/13 10:11 PM (10 years, 3 months ago) |
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I've had 1/2 pint brf jars colonized fully in a little over 2 weeks before but they were the short wide mouth jars and they always colonized much quicker for me than those tall jars your using.
When I was doing cakes I would say my average was 3 to 4 weeks for the short wide mouth jars and 4 to 5 weeks for the tall half pints
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 21 days
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Re: first grow 12 days after inoc [Re: farmerofduck]
#18988927 - 10/16/13 11:04 PM (10 years, 3 months ago) |
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Quote:
farmerofduck said: 12 days just seems a long time to only have tiny spots of growth. Maybe my substrate mix is off? What is the main factor for mycelium growth rate?
Did you boil all of these jars at the same time? Did you steam sterilize, or PC?
The bottom set of pictures looks really wet, and the top set looks good. If your growing Cubes, just expect to get on their schedule. They will grow when they are ready, they don't hurry up for us.
I like to get really into my growth cycle, put all of that energy into the sterility process, then get really into another hobby and forget about my jars, and bags. In a few weeks, you will be pleasantly surprised at how far along they have come.
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NecroMyce
Mostly Ghostly



Registered: 05/12/13
Posts: 747
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Re: first grow 12 days after inoc [Re: N2ocean]
#18990287 - 10/17/13 10:36 AM (10 years, 3 months ago) |
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Quote:
N2ocean said: Yea, i'd be patient, brf jars take a while. I've had plenty of batches take forever, well over a month to colonize, I've got a batch of golden teachers that's sooooo slow right now. I inoculated them on 9/8 and they're about 2/3-3/4 done. I have some ecuadors that i did on the same day that have been done for a couple weeks now, I gotta birth them but I'm lazy and I've got these in an incubator at perfect conditions and everything... The other 1/2 pint jars were mentioned and in my experience i've had much better success and faster/more even colonization with the shorter wider jars. I really suggest getting some of these for your next batch... Also, i've found that temps one of the biggest factors for colonization speed, you're reporting good temps, so not to worry, but if anything's gonna slow down growth, it's less than optimal temps.
Also, i'm guessing you got these spore syringes off a website. Another thing i've experienced is super slow colonization from any syringe I order. Make spore prints from this batch, and i like mine dark with spores and have found that these always colonize much faster! Most websites put as few spores as possible in each syringe. Granted you don't need a syringe black w spores, but it doesn't hurt if you have a good amount in them.
Patience, they are looking good so far... 
p.s. you mentioned that some people claim 14 day full colonization. meh, i've never had that w/ spore prints onto brf jars. Even w Grain to Grain transfers 14 days is good... Don't worry too much about not hitting these "benchmarks" that people claim. most of them are exaggerated by at least a little bit anyway.
yeah usually mine take about a month to fully colonize or even get close. I ordered 4 different syringes online and the Penis Envy were about 80% within almost 2 weeks. Where as some of the other jars hadnt even shown any signs and some were barley sparking life. If i was impatient about the slow colonization usually ill just start another batch and to keep my attention span controlled hahad
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farmerofduck
Stranger


Registered: 07/22/13
Posts: 28
Last seen: 10 years, 3 months
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Re: first grow 12 days after inoc [Re: NecroMyce]
#18990480 - 10/17/13 11:18 AM (10 years, 3 months ago) |
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Thanks for the detailed reply. I got my syringes from THE maybe its slow because they're golden teachers? I'm gonna go out and try to find the wide mouth jars this weekend and use them on the next batch. I tried to find them before but they weren't in stock.
What's the next best and easiest tek next to pf? For jars not bulk...
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N2ocean
Picture-er


Registered: 03/19/13
Posts: 42
Last seen: 8 years, 11 months
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Re: first grow 12 days after inoc [Re: farmerofduck]
#18993051 - 10/17/13 09:03 PM (10 years, 3 months ago) |
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Well if you have a pressure cooker, you could start on grain jars. They can be inoculated w a spore syringe same as the brf tek. Fruiting them is the more challenging part. You can use your same SGFC as well. Which i recommend at least starting out. Read up some teks on grain, i wish i could give you some recent up to date ones, but most of the info from some of the older teks holds true.
My preference.
Use rye, it's easier to get the moisture content right over wild bird seed. Soak the rye for 24 hrs. then simmer for 20min to a half hour, and you'll have the rye grain at the right moisture to put in 1 qt jars. rye berries that's where i get my stuff. amazon's the best, it's $30 w/shipping, so more expensive than wbs, but better and easier. I'm sure you can find cheaper if you get it locally in store.
For fruiting you'll want to read up fairly heavily on what exactly fruiting entails, what causes the substrate to begin the fruiting process, and how you can mimmick these conditions. *important research topic
You won't have to use manure but you will be mixing your grain jars after they're fully colonized onto another substrate medium. Commonly a mix of cococoir and verm. You'll then have to wait for that to colonize and then you'll be placing this substrate in fruiting conditions.
I suggest in the beginning to mix your grain jars and coco/verm substrate in containers that will fit inside your current SGFC, it won't require a huge amount of substrate and it's something you're familiar with. If your current SGFC holds moisture well and causes your BRF cakes to fruit then it's a solid fruiting chamber and you don't have to worry about a constructing a bad "new" fruiting chamber.
That's my advice for "moving on" keep it as simple as possible, and stick to as many familiar processes as you can.
For your response to golden teachers being slow, personally i think they are.
Also, I should probably have put this up higher because i think it's important: Do at least 3-5+ more batches of BRF cakes. Become familiar with the mushroom lifecycle, perfect your sterile techniques!!!! keep researching all of this time about what you want to do next. Don't rush things. RR's videos mushroomvideos.org are really informative.
Also, work with spore prints and creating your own syringes, it will make you pay extra attention to sterility, save you money, and give you better syringes. It will also familiarize yourself with mushroom biology. Look up teks on how to properly capture a spore print!
Once you are comfortable doing the BRF tek and propogating a spore line, then I'd say start into grain teks.
hope this helps
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