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OfflineJahLambsbread
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Registered: 08/08/10
Posts: 684
Last seen: 6 years, 4 months
Mycelium growth on clones
    #18967810 - 10/12/13 10:39 AM (10 years, 4 months ago)

I left four pieces of the middle of a mushroom cap in a quart jar for a few days.  Now there is a lot of mycelium. I would show pictures but I am not sure how to do that yet.


MY question is should I put this into a half pint mixed with brown rice flour, vermiculite, and distilled water just like when you inoculate it with a syringe?

Or should I try to make an agar plate(I don't have petri dishes only pyrex dishes right now)?


The agar plate seems like it is a waste because the mycelium is already growing good. I would rather put it in a half pint jar but I am no expert.


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Offlinekrypto2000
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Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
Re: Mycelium growth on clones [Re: JahLambsbread]
    #18967971 - 10/12/13 11:31 AM (10 years, 4 months ago)

It really doesn't matter, though what are you going to do with a single half pint? Is your goal to grow a single cake or do you want to expand it? Also it depends how clean the culture is, if it's clean then do whatever you want with it, if it's dirty there's no point in doing anything but agar. Also what do you mean you put it in a quart jar? An empty quart jar? It might just be growing where there is moisture, I wouldn't necessarily assume it's clean just because mycilium is all that you see, if there's hardly any nutrients in there there might be contaminants growing on the mycilium itself and around it which you just can't see. You normally don't want to close from the cap either, but from the stipe(sp?) which is part of the stem.


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
Posts: 10,573
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Re: Mycelium growth on clones [Re: JahLambsbread]
    #18968247 - 10/12/13 01:07 PM (10 years, 4 months ago)

You asked this already:

Quote:

JahLambsbread said:
three pieces of the middle of the cap were taken and put in a quart mason jar with nothing in it.
It was just being stored untill agar was obtained but mycelium already started to grow.

What is the next step to do now? because being low on spores is not good:(

If It is put in a half pint jar with pre mixed vermiculite; brown rice flower; and water will the mycelium spread?




Double posts are against forum rules :rules:


--------------------

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Here is how I get things done.
You should take a look. :hehehe:


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