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Offlineghostshadow422
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Registered: 10/02/13
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2 Different strains, same LC
    #18966637 - 10/12/13 12:59 AM (10 years, 3 months ago)

Hi guys this is my second grow, last grow was 2 years ago so restarting for scratch. That being said i ordered 4 strains of cube, huatla, b+, Amazon, and golden teacher. I decided to go straight to a multi spore LC of b+ and amazon. So I made the b+ LC and the golden teacher (1Cc) per cup of water. then proceeded to knock up the jars with the rest of the syringes. I ended up with a small amount of amazon left (prob another Cc or so), and not really thinking of the effects I shot it into the LC which contained the golden teacher. Well this all happened approx. 3 days ago. At this point the b+ LC is showing good signs of growth, but the other LC ( GT and Amazon) seems to be showing very litte growth, but definitely something. So I'm wondering are the GT and Amazon slow colonizers? Should I not have mixed spores? Not trying to be redundant, because I know this is asked a lot, but when should I expect pf tek growth? I have small mycelium spots on the b+ jars, but coincedentily, none on the Gt or Amazon jars. Is b+ just a very fast colonizer and is/or Gt Amazon slow? I have not touched the huatla spore syringe yet, I received my supplies from sporeworks. Wow rambled more than I realized, I hope I kept my post coherent though, thanks in advance for any ideas,
thoughts that may be shared with me. -ghost :eek:


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Offlineboommer
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Re: 2 Different strains, same LC [Re: ghostshadow422]
    #18966673 - 10/12/13 01:17 AM (10 years, 3 months ago)

FALLOW THE RULES...
1.  Please use the search posts function before asking a question.  Your question has already been asked and answered hundreds of times, so don't think you're going to get a better answer by asking it again.  In the 'search posts' link at the top of the page, you can select an individual user or the trusted cultivator group as a whole to filter results to come only from them.

3.  Spores are sold for microscopy use only so mycologists can ID mushrooms. If you're going to misuse them to grow a controlled substance, keep it to yourself.  Any post which mentions 'such and such' vendor, will be deleted and you'll receive an official warning.  Do not plug non sponsors.


with that said i searched it for you http://www.shroomery.org/forums/showflat.php/Number/4375637#4375637
also strains make little difference in colonization speed its more about that spores genetics inherited... i believe whats happening in ur lc is both strains are trying to be dominant and take over... start over is my suggestion.. i mean u have 40ccs on spores.


Edited by boommer (10/12/13 01:22 AM)


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OfflinePussyFart
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Re: 2 Different strains, same LC [Re: ghostshadow422]
    #18966765 - 10/12/13 02:01 AM (10 years, 3 months ago)

Quote:

ghostshadow422 said:
So I'm wondering are the GT and Amazon slow colonizers? Should I not have mixed spores?



No, there is no variety that is known to colonize faster or slower than any other.

And mixing spores is ok, they are all the same species.

Quote:

ghostshadow422 said:
Not trying to be redundant, because I know this is asked a lot, but when should I expect pf tek growth?




Not sure what pf tek growth is, but why not just inject spores into the cakes, and forget about the LCs.

This way you eliminate that whole grey area of LC, contams, and time wasted.

Quote:

ghostshadow422 said:
I have small mycelium spots on the b+ jars, but coincedentily, none on the Gt or Amazon jars. Is b+ just a very fast colonizer and is/or Gt Amazon slow?



Neither, they are all the same in the fact that they will always be unpredictable.


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Offlineghostshadow422
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Re: 2 Different strains, same LC [Re: PussyFart]
    #18967825 - 10/12/13 10:46 AM (10 years, 3 months ago)

because I wanted to b able to knock up more jars than the syringe 10cc would allow


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Offlineghostshadow422
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Re: 2 Different strains, same LC [Re: ghostshadow422]
    #18967834 - 10/12/13 10:49 AM (10 years, 3 months ago)

looking to inoculate all the jars I have


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OfflinePussyFart
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Re: 2 Different strains, same LC [Re: ghostshadow422]
    #18967981 - 10/12/13 11:33 AM (10 years, 3 months ago)

Then just use a few drops of spore solution per hole, per jar.

It is really that easy....stretch that shit son!


Edited by PussyFart (10/12/13 11:34 AM)


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Offlineghostshadow422
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Re: 2 Different strains, same LC [Re: PussyFart]
    #18969237 - 10/12/13 05:45 PM (10 years, 3 months ago)

yep, I did, trying to shoot up 60-70 jars lol


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OfflineSchmendrick
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Re: 2 Different strains, same LC [Re: ghostshadow422]
    #18969469 - 10/12/13 06:51 PM (10 years, 3 months ago)

Quote:

ghostshadow422 said:
yep, I did, trying to shoot up 60-70 jars lol




That's ~ 1/30th cc per hole (if you are doing 4 holes per jar.  I'm no expert, but I think those jars will take longer than normal to colonize :smile:  Better make sure your sterile technique is flawless.


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OfflinePussyFart
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Re: 2 Different strains, same LC [Re: Schmendrick]
    #18969540 - 10/12/13 07:10 PM (10 years, 3 months ago)

Less spore solution does not mean slower colonization times....not at all.


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OfflineSchmendrick
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Re: 2 Different strains, same LC [Re: PussyFart]
    #18969570 - 10/12/13 07:19 PM (10 years, 3 months ago)

Quote:

Notahacker420 said:
Less spore solution does not mean slower colonization times....not at all.




Not normally... 1cc of spore solution will colonize as fast as 2cc, but 1/30th cc is a REALLY small amount (~2/3 of a drop);  that's not even enough to break it's own surface tension and run down the side of the jar.  Common sense tells me that a 1cc dose which runs to the bottom of the jar depositing it's spores at multiple points along the way will probably colonize faster than a 1/30th cc dose which will just sit at the inoculation point.

Maybe I am wrong though... they are called "magic" mushrooms after all. Maybe they can do all sorts of things that defy common sense.

It's all academic anyway since I seriously doubt OP has the necessary muscle control to consistently distribute such a small dose 280 times in a row (70 jars X 4 holes each). 


Edited by Schmendrick (10/12/13 07:38 PM)


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Offlineghostshadow422
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Re: 2 Different strains, same LC [Re: Schmendrick]
    #18972313 - 10/13/13 02:10 PM (10 years, 3 months ago)

so the point is, you don't think I should make a few lc jars, but instead try to stretch 4 syringes into 60 jars? I dunno, I think the LC is a more efficient way to go, but that's beside the point, my question was whether 2 different strains put into the same LC would have problems, because it doesn't seem to be doing anything, the jars I knocked up are starting to colonize though at least, even though the temp here fluctuates a lot, hasn't been a week yet though, so should be all good. I'm gonna just LC the last syringe I have into 5 jars, so I can have enough stuff to knock up the rest of the jars


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Invisible36fuckin5
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Registered: 08/11/03
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Re: 2 Different strains, same LC [Re: ghostshadow422]
    #18972523 - 10/13/13 03:09 PM (10 years, 3 months ago)

The point is that if your LCs don't work out, which chances are decent they won't, then you just waited a month and wasted everything you have.

If you'd have just knocked up some jars, you could fruit them and make spore prints.

Your LC is slow because there are too many spores in it, or there's some contam. Too much competition. You're only supposed to use a drop to inoculate it.


--------------------
Redd Foxx said:
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Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Offlineghostshadow422
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Re: 2 Different strains, same LC [Re: 36fuckin5]
    #18973278 - 10/13/13 05:54 PM (10 years, 3 months ago)

point is, the second one is finally going now, and I got the other 4 jars going with LC as well, what is 1cc per LC too much? that's how much I put in each jar except for the one in question, so there was only 2 CCs in that ( 1 cc of each strain ). I am going to do spore prints for more lc once the cakes are done, I have 4 lc going now and 36 knocked up jars, so even if the one LC doesn't go through, I didn't waste a bunch of time, does that sound good?


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