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InvisibleWilly Wonka
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Pin to agar, to agar, to LC?
    #18965013 - 10/11/13 05:39 PM (10 years, 3 months ago)

I am going to clone a pin from a cluster growing in a mono tub.  I am going to grab the 1st pin I see see pop up from a cluster in the tub.  I am going to clone the pin on agar.  Once new mycelium from the pin begins to grow I am going to transfer it to a new dish.  Now here is where my question comes up.  Once P2 begins to grow out can I use the agar culture to inoculate a liquid culture and expect the cloned genetics to grow in the LC or will throwing the P2 culture into an LC allow for multi strains to grow thus negating the cloning work I already did?


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Invisiblelaughingsol
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Re: Pin to agar, to agar, to LC? [Re: Willy Wonka]
    #18965075 - 10/11/13 05:53 PM (10 years, 3 months ago)

Quote:

Willy Wonka said:
Once P2 begins to grow out can I use the agar culture to inoculate a liquid culture and expect the cloned genetics to grow in the LC or will throwing the P2 culture into an LC allow for multi strains to grow thus negating the cloning work I already did?




Was the culture previously isolated? or did you begin your monotub from a multispore inoculation?

If the pin is from a monotub inoculated via multispore methods, you may very well create a multi strain LC.  But I don't see how that would negate the cloning work you have done- you will still have the mycelium that you cloned, whether its a single strain or multiple strains, growing in your LC.


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InvisibleWilly Wonka
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Re: Pin to agar, to agar, to LC? [Re: laughingsol]
    #18965583 - 10/11/13 08:12 PM (10 years, 3 months ago)

The pin is from a multispore inoculation.  When searched the subject I found this:
Quote:

RogerRabbit said:
Pins are rapidly growing. It matters not if they've been exposed to the air since the mycelium will outrun any contaminants.  You simply take a small wedge from the leading edge of the mycelium and transfer it to a new plate.  The P2 plate is the one you use to inoculate grains, not the P1, which will have any contaminants from the original clone.
RR



After I read this I got the impression that I would be able to use P2 to inoculate either my master grain jar or my LC. There reason I am cloning a pin from a cluster is because I would love to grow a tub that pins from end to end. All the tubs I have grown are really hit or miss and I have never had a tub that was covered.  I figure until I start working with agar I wont get there.  Do I need to work my culture down to an isolate?


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OfflineRogerRabbitM
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Re: Pin to agar, to agar, to LC? [Re: Willy Wonka]
    #18965660 - 10/11/13 08:27 PM (10 years, 3 months ago)

Pins can have multiple strains, but that doesn't mean they always do.

If you have a clean culture on agar, I wouldn't take a chance on screwing it up with LC.  Simply inoculate a grain master with the wedge, and then do grain to grain transfers to get all the spawn you want.  It's far faster than LC.
RR


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InvisibleWilly Wonka
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Re: Pin to agar, to agar, to LC? [Re: RogerRabbit]
    #18965725 - 10/11/13 08:40 PM (10 years, 3 months ago)

Cool, thank you sir:wink:


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