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Offlinegreenery1
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Is this Mycelium (or what) in LC? (pictures)
    #18964286 - 10/11/13 02:33 PM (10 years, 3 months ago)

LC with honey, there was a lot of gunk in the batch (first time), and then that white blob that looks like a little fish there...




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OfflinePussyFart
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #18964297 - 10/11/13 02:35 PM (10 years, 3 months ago)

There is no way to visually tell if an LC is good or bad, test jars must be shot up and grow out.

That being said, that does not look healthy.


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OfflineMr. Wilson
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: PussyFart]
    #18964310 - 10/11/13 02:37 PM (10 years, 3 months ago)

dude looks like hes got a nice chunk of caramel in there


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Offlinegreenery1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Mr. Wilson]
    #18964322 - 10/11/13 02:40 PM (10 years, 3 months ago)

The white blob is covered in the brown caramel looking flakes, but the white blob is getting bigger...this is 2 weeks from inoculation, GTs, three other  jars nothing. The glass is corrugated which makes it harder to see clearly.


Edited by greenery1 (10/11/13 02:42 PM)


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Offlinespacechildo
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #18964475 - 10/11/13 03:14 PM (10 years, 3 months ago)

Looks like you trapped an orange frog in the first picture!
Been sitting by my computer all day, a bit slow in the head, and was like whaaat? animal cruelty?
Figured no, just my vivid imagination.


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Offlinegreenery1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: spacechildo]
    #18964531 - 10/11/13 03:28 PM (10 years, 3 months ago)

I thought it looked like a little puffer fish or a bull-head. Anyways I thought mycelium grew like a web through out, this is more like an expanding mucus-like blob. I was going to toss the whole batch, its been two weeks, then noticed this a few days ago. Or maybe its an adenoid?


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Offlinegreenery1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #18996152 - 10/18/13 03:31 PM (10 years, 3 months ago)

Still growing. I thought the caramelized gunk was just getting stuck in its fur, and it was I guess, but I see now that it's eating it, my LC is clearer and the gunk is disappearing. Those are some hungry GT spores! Anyone still in doubt if this is mycilium? Thanks.



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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #18996174 - 10/18/13 03:38 PM (10 years, 3 months ago)

Its probably mycelium. Now whether it's cubensis mycelium. . .


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Offlinegreenery1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Pastywhyte]
    #18996230 - 10/18/13 03:54 PM (10 years, 3 months ago)

ahh, another snag in the plot...so what other kinds of mycilium could it be?


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InvisiblePestile

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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #18996268 - 10/18/13 04:01 PM (10 years, 3 months ago)

You need to knock up a jar and see what color it brings forth. :wink:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #18996276 - 10/18/13 04:03 PM (10 years, 3 months ago)

Quote:

greenery1 said:
ahh, another snag in the plot...so what other kinds of mycilium could it be?




Quote:

Pestilence said:
You need to knock up a jar and see what color it brings forth. :wink:




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Invisiblespaceman101
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Re: Is this Mycelium (or what) in LC? (pictures) *DELETED* [Re: Pastywhyte]
    #18996748 - 10/18/13 06:14 PM (10 years, 3 months ago)

Post deleted by spaceman101

Reason for deletion: oops



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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #18996757 - 10/18/13 06:16 PM (10 years, 3 months ago)

That's some serious pruning were you swimming or just got out of a long bath:ass:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: spaceman101]
    #18996764 - 10/18/13 06:17 PM (10 years, 3 months ago)

so what is it no way to know until you knock up some jars...this is why i don't do LC's...


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: blueconfusion]
    #18996933 - 10/18/13 07:07 PM (10 years, 3 months ago)

Quote:

blueconfusion said:
so what is it no way to know until you knock up some jars...this is why i don't do LC's...



:whathesaid:

I'm actually thinkin about giving LC another go soon. Got my technique in my SAB down pretty good, and LC would have some advantages for species like pans and p mexicana. But there is no way I would think about inoculating said LC with anything but a agar wedge.


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Pastywhyte]
    #18997398 - 10/18/13 08:37 PM (10 years, 3 months ago)

Send me a syringe and I'll test it for you.


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OfflinePussyFart
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Enigma1]
    #18998324 - 10/19/13 01:48 AM (10 years, 3 months ago)

Quote:

Enigma1 said:
Send me a syringe and I'll test it for you.



:lmafo:


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Offlinegreenery1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: PussyFart]
    #18999266 - 10/19/13 11:16 AM (10 years, 3 months ago)

ah...I wasn't aware that mycilium could be contaminated even if it is growing.


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #18999302 - 10/19/13 11:27 AM (10 years, 3 months ago)

Oh, it can. On agar, in a grain jar, LC.


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Pestile]
    #18999569 - 10/19/13 12:34 PM (10 years, 3 months ago)

But are you sure thats mycelium growing and not say cobweb?


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: blueconfusion]
    #18999571 - 10/19/13 12:34 PM (10 years, 3 months ago)

Just a for instance


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Offlinegreenery1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: blueconfusion]
    #19000471 - 10/19/13 04:32 PM (10 years, 3 months ago)

I was hoping someone here could tell me that! :smile:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19000659 - 10/19/13 05:24 PM (10 years, 3 months ago)

knock up some brf or grain...:shrug:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: blueconfusion]
    #19001495 - 10/19/13 08:53 PM (10 years, 3 months ago)

Quit being scared and test it . If it's contaminated it's contaminated.


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Offlinegreenery1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Enigma1]
    #19002887 - 10/20/13 07:25 AM (10 years, 3 months ago)

need to get vermiculite, local hydroponics only sells it in huge amounts...


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19002969 - 10/20/13 08:07 AM (10 years, 3 months ago)

i buy huge bags http://www.menards.com/main/building-materials/insulation/loose-fill/vermiculite-insulation/p-1485908-c-5777.htm here it's actually the same stuff nothing nasty and costs less than if you were to buy it in gardening section...i compared:dancer:


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Offlinegreenery1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: blueconfusion]
    #19003008 - 10/20/13 08:28 AM (10 years, 3 months ago)

Hmm thats actually about the same size, but here it is 40USD. They also have perlite the same size, said they would give me half of each if I wanted. I guess you do go through it pretty fast so maybe I'll just go for that.


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19003037 - 10/20/13 08:43 AM (10 years, 3 months ago)

like i said $40 at the hydro store (everything will be marked up there) or $14 in the building materials section...just gotta do a little looking is all :shrug:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19003084 - 10/20/13 09:03 AM (10 years, 3 months ago)

Quote:

greenery1 said:
ah...I wasn't aware that mycilium could be contaminated even if it is growing.



LCs can carry contamination even if they are fluffy white myc growing in a crystal clear solution and must be  grown out to test.

I've had LCs that look like yours from carmelization of the sugars in the PC that grew clean  and I've had them that look like yours that were dirty
Even with a microscope contamination may not be visible. I've had bacteria stowe away within the mycelium, where it  grew clean looking on agar and spoiled every jar of grain I put it in.


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19003125 - 10/20/13 09:20 AM (10 years, 3 months ago)

just to reiterate LCs are flawed especially when doing MS with a cube.  if you want more spawn do G2G transfers it's a hard lesson to learn but there is not as much guessing, or if you can do MS to agar which is even better do that.  LCs in theory are great and very appealing but if you don't have your technique down i wouldn't recommend that, cause now really all you can do is grow out what you have and hope for the best...


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Offlinegreenery1
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: blueconfusion]
    #19004318 - 10/20/13 03:01 PM (10 years, 3 months ago)

I was originally going to do MS to agar instead of LC, but I was reading that agar is for strain isolation, and LC would be better if you just wanted to give your BRF a jump start when inoculating. I see now that is just as risky as just knocking up the BRF directly?

After BRF i was going to go to casing, my first go at it so I'm just using a pot at the moment to sterilize the LC and BRF cakes. If successful will invest in a PC, but if unsuccessful, um, will also invest in a PC... :smile:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19004634 - 10/20/13 04:16 PM (10 years, 3 months ago)

If you made LC without a PC, it's not very likely to be clean :frown:
Not having a PC kinda restricts you to the PF tek using MS syringes made from spore prints.

If you wish to go further in the hobby you'll need about a 20q PC
It's a $100 investment that you will never regret, I wore out my $100 Presto and picked up a $325 AA. I know it's wrong to have feelings for inanimate objects, but I still get a little chubber when using that "All American 930"


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19004683 - 10/20/13 04:28 PM (10 years, 3 months ago)

ah i see, thanks. I used a rice cooker (giving about 15 psi?). i was sure I read that LC could also be done that way, along with PF.


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OfflineISuckAtHalo
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19004697 - 10/20/13 04:31 PM (10 years, 3 months ago)

Quote:

skippydude said:
, but I still get a little chubber when using that "All American 930"



:whatwhatwhat:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19004728 - 10/20/13 04:40 PM (10 years, 3 months ago)

Quote:

skippydude said:
If you made LC without a PC, it's not very likely to be clean :frown:





Actually, the ingredients we use for LC are not filled with different types of endospores and such, so we don't have to pressure cook liquid cultures. A simple 30 min. boil will do. :smile:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19005041 - 10/20/13 06:01 PM (10 years, 3 months ago)

Quote:

greenery1 said:
ah i see, thanks. I used a rice cooker (giving about 15 psi?). i was sure I read that LC could also be done that way, along with PF.



If you are at 15 psi then you are indeed pressure cooking :thumbup:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Pestile]
    #19005075 - 10/20/13 06:06 PM (10 years, 3 months ago)

Quote:

Pestilence said:
Quote:

skippydude said:
If you made LC without a PC, it's not very likely to be clean :frown:





Actually, the ingredients we use for LC are not filled with different types of endospores and such, so we don't have to pressure cook liquid cultures. A simple 30 min. boil will do. :smile:



If ingredients are exposed to air at anytime during the process, prior to sterilization, then they have been exposed to endospores.


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19005161 - 10/20/13 06:23 PM (10 years, 3 months ago)

The point is that there is no grain core that needs to be heated up so pressure is not needed to sterilize the LC.
I could find a RR quote somewhere, but I really don't want to. :smile:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Pestile]
    #19005349 - 10/20/13 06:56 PM (10 years, 3 months ago)

An endospore has a hard outer shell on it that requires 251F to penetrate wether it is in, on or around anything, grain has nothing to do with it.
These things survive space travel and boiling water won't kill all of them.
If your not having issues I'd say are working in a pretty sanitary environment and have been lucky.


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19007111 - 10/21/13 04:39 AM (10 years, 3 months ago)

Quote:

skippydude said:
An endospore has a hard outer shell on it that requires 251F to penetrate wether it is in, on or around anything, grain has nothing to do with it.
These things survive space travel and boiling water won't kill all of them.
If your not having issues I'd say are working in a pretty sanitary environment and have been lucky.




You are right. I had some info mixed up, sorry about that. :smile:


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Offlinespacechildo
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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19007290 - 10/21/13 06:49 AM (10 years, 3 months ago)

Quote:

skippydude said:
Quote:

Pestilence said:
Quote:

skippydude said:
If you made LC without a PC, it's not very likely to be clean :frown:





Actually, the ingredients we use for LC are not filled with different types of endospores and such, so we don't have to pressure cook liquid cultures. A simple 30 min. boil will do. :smile:



If ingredients are exposed to air at anytime during the process, prior to sterilization, then they have been exposed to endospores.




What about pf-tek jars? they get exposed to open air when you mix verm and brf.
they still do fine without a pc..


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: spacechildo]
    #19007325 - 10/21/13 07:10 AM (10 years, 3 months ago)

Quote:

spacechildo said:
Quote:

skippydude said:
Quote:

Pestilence said:
Quote:

skippydude said:
If you made LC without a PC, it's not very likely to be clean :frown:





Actually, the ingredients we use for LC are not filled with different types of endospores and such, so we don't have to pressure cook liquid cultures. A simple 30 min. boil will do. :smile:



If ingredients are exposed to air at anytime during the process, prior to sterilization, then they have been exposed to endospores.




What about pf-tek jars? they get exposed to open air when you mix verm and brf.
they still do fine without a pc..



PF jars are pasteurized, A process that leaves behind beneficial bacteria which battles the trich for you


Edited by skippydude (10/21/13 07:14 AM)


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19007336 - 10/21/13 07:17 AM (10 years, 3 months ago)

PF jars are sterilized not pasteurized


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Stromrider]
    #19007339 - 10/21/13 07:19 AM (10 years, 3 months ago)

Quote:

Stromrider said:
PF jars are sterilized not pasteurized



They have to reach temps of 251F to be considered sterilized>
How are you doing that on the stove top?


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Stromrider]
    #19007341 - 10/21/13 07:19 AM (10 years, 3 months ago)

Quote:

If ingredients are exposed to air at anytime during the process, prior to sterilization, then they have been exposed to endospores.




This is not really accurate


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19007345 - 10/21/13 07:21 AM (10 years, 3 months ago)

You can steam sterilize but, it takes about a week to kill all the endospores. They have to germinate to be killed.


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19007347 - 10/21/13 07:22 AM (10 years, 3 months ago)

Quote:

skippydude said:
Quote:

Stromrider said:
PF jars are sterilized not pasteurized



They have to reach temps of 251F to be considered sterilized>
How are you doing that on the stove top?




You don't have to reach temps of 250 to sterilize. Killing endospores requires these high temps and grains are usually heavily laden with them and that is why we have to pc them. Things like brf and verm aren't full of endospores therefore they don't require those high temps for sterilization


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Stromrider]
    #19007354 - 10/21/13 07:24 AM (10 years, 3 months ago)

Quote:

Stromrider said:
Quote:

If ingredients are exposed to air at anytime during the process, prior to sterilization, then they have been exposed to endospores.




This is not really accurate



???
Open air = possible endospores, anyday of the week!
What is this test Skippy day? lol


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19007356 - 10/21/13 07:25 AM (10 years, 3 months ago)

Possible yes
Probable no


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Stromrider]
    #19007360 - 10/21/13 07:28 AM (10 years, 3 months ago)

Quote:

Stromrider said:
Possible yes
Probable no



Well said


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19007367 - 10/21/13 07:32 AM (10 years, 3 months ago)

:smile: thanks I enjoyed our discussion


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude] * 1
    #19007594 - 10/21/13 09:18 AM (10 years, 3 months ago)

Quote:

skippydude said:

If ingredients are exposed to air at anytime during the process, prior to sterilization, then they have been exposed to endospores.




brf+verm is mixed in open air. they do get sterilized by steaming in a pot.
if your statement was true pf-tek would need a pc too, wouldn't it?

btw,
Quote:

skippydude said:
You can steam sterilize but, it takes about a week to kill all the endospores. They have to germinate to be killed.




it takes 8 hrs to steam grains in a normal pot.


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: spacechildo]
    #19007652 - 10/21/13 09:43 AM (10 years, 3 months ago)

Quote:

spacechildo said:
it takes 8 hrs to steam grains in a normal pot.




Still not a good idea. I say get a job, save some money, and buy a pc you lazy hippy! :lol:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Stromrider]
    #19007704 - 10/21/13 10:01 AM (10 years, 3 months ago)

Back to the original question
Here is a LC that looks very similar to yours

Made with LME & Dextrose which caramelized a bit in the PC
It tests clean, so as previously stated, "You gotta throw some on grain to know for sure" :goodluck:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19007715 - 10/21/13 10:05 AM (10 years, 3 months ago)

True there is only one way to know 4 sure


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Stromrider]
    #19008689 - 10/21/13 02:00 PM (10 years, 3 months ago)

:popcorn:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Enigma1]
    #19032360 - 10/25/13 06:08 PM (10 years, 3 months ago)

Knocked up some BRF today, what I discovered was that the mycilium gets stuck in the syringe when trying to suck it up (18 gauge needle), I couldn't! had to take water from around it - I did do 4 new LC's last weekend and was pleased to see the two new GT jars both producing MC in 5 days. So I used those for the inoculation, and did 1 using that old GT in the original pics as a test.

So I guess one question: is the MC spread throughout the whole jar, or can you miss it if not stuck into the MC cloud?

Another issue that I realized is if you are sucking up 8-10 CC for a few jars of BRF, your supply runs out fairly quick...so I'm going to make a bigger jar of LC, also tipping the jar to reach the water is a bit disconcerting...

When mixing the VErm with water 2:1, I found quite a bit of water came out when I squeezed a handful, so I add more Verm, was still a problem, but then I was wondering if I wasn't squeezing it too hard, first time, so not sure if it was ok or not. It seemed fine after adding the BRF.


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19032497 - 10/25/13 06:40 PM (10 years, 3 months ago)

when i started making large LCs is when i ran into problems...


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: blueconfusion]
    #19032909 - 10/25/13 08:02 PM (10 years, 3 months ago)

Put a marble or a piece of broken glass in your LC jug, this will help, when shaking the jar, to break up the mycelium mass when ready for use.
If you don't get a little of the white stuff in each item you knock up, it is possible that nothing will grow. I've had it happen on grain and petri dishes.
Go to a 14 gauge needle if you can, at least 16 gauge for LCs as 18s clog very easy.

I use a heavy duty inoculation port, an 18 gauge needle with a .02 micron Whatman syringe filter for GE. Then draw the cultures with a separate 14g or 16g
I have a magnetic stir plate so I now use that to break up the myc. Before that I was able to shake these jars with a marble in it without wetting the filter(bacteria swims right through a wet GE filter)


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19043594 - 10/28/13 12:17 AM (10 years, 3 months ago)

Thanks Skippy. Most of my jars are growing after 2 days, a few had nothing so I re-shot them with MC, checked the syringe before to make sure I could see bits floating around it.

Is that a homemade port there in the picture? Haven't seen one like that.

I was planning to do bulk from the BRF cakes, any suggestions on a tek to follow? I was going to try Coir/Verm...


Edited by greenery1 (10/28/13 01:30 AM)


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19043725 - 10/28/13 12:49 AM (10 years, 3 months ago)

Quote:

skippydude said:






Brilliant! Great idea, man. :thumbup:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19044305 - 10/28/13 04:18 AM (10 years, 3 months ago)

Quote:

greenery1 said:
Thanks Skippy. Most of my jars are growing after 2 days, a few had nothing so I re-shot them with MC, checked the syringe before to make sure I could see bits floating around it.



You reinoculated after only 2 days of no growth?

Spores can take up to 2 weeks to germinate in some cases...


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: PussyFart]
    #19044699 - 10/28/13 08:36 AM (10 years, 3 months ago)

sorry it was from an LC, the other jars all had growth from the same LC, I was concerned that I might have missed the MC when drawing up, it was my first time :smile:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19047164 - 10/28/13 05:01 PM (10 years, 3 months ago)

Here's that original LC that I posted in this thread, the one jar that I tested here is nearly fully colonized in three days, where as the other jars (in the next post below) are a bit slower (from a younger LC) but both taken from the same spore print.



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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19047207 - 10/28/13 05:06 PM (10 years, 3 months ago)

Here's 2 jars which are just getting going after 3 days from a younger LC with GT mycillium.



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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19047494 - 10/28/13 05:41 PM (10 years, 3 months ago)

They look a little nasty, IMO.


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Pestile]
    #19047695 - 10/28/13 06:09 PM (10 years, 3 months ago)

:whathesaid:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Pastywhyte]
    #19047738 - 10/28/13 06:15 PM (10 years, 3 months ago)

They look fine.
Not very rhizo, but no sign of contamination from what I can see.
Odd ball choice on vessel size & shape for the PF tek? :takingnotes:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Pastywhyte]
    #19047786 - 10/28/13 06:25 PM (10 years, 3 months ago)

Here's that first glass in natural outside light, 13ml glasses:



Edited by greenery1 (10/28/13 06:27 PM)


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19047795 - 10/28/13 06:27 PM (10 years, 3 months ago)

Looks healthy to me.....


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19047837 - 10/28/13 06:33 PM (10 years, 3 months ago)

Quote:

greenery1 said:





These were the ones that looked kinda gross IMO. Looks like they have a greenish tinge which is probably just the lighting.


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Pastywhyte]
    #19047918 - 10/28/13 06:47 PM (10 years, 3 months ago)

I was going to go bulk next, Coir/Verm, for the sake of increasing yields, and because it seems quite simple - any advise here?

BTW, I always see '1 brick' of Coco used in teks, here I buy it loose in a bag, how much/quantity is '1 brick'?

Thanks for the feedback folks!


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Pastywhyte]
    #19047935 - 10/28/13 06:50 PM (10 years, 3 months ago)

Quote:

Pastywhyte said:
Quote:

greenery1 said:





These were the ones that looked kinda gross IMO. Looks like they have a greenish tinge which is probably just the lighting.



:whathesaid:


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: blueconfusion]
    #19047938 - 10/28/13 06:50 PM (10 years, 3 months ago)

7-8 quarts


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: blueconfusion]
    #19047941 - 10/28/13 06:51 PM (10 years, 3 months ago)

1 brick = 650 grams give or take.


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: Pastywhyte]
    #19081044 - 11/03/13 03:48 PM (10 years, 2 months ago)

Just a question about tubs, was wondering if I could try something like in the picture? 4 stacked drawers, I notice some of these tubs and drawers have a strong smell from the material they are made from (burnt tires or something) should avoid these? they are always the cheapest.

I will be spawning to bulk soon (verm/coir/coffee grounds), GT's, does the lid need to be airtight? Dark non-see-through tub? I have been searching the site for answers but so often find conflicting opinions, so thought I ask here. Thanks



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Re: Is this Mycelium (or what) in LC? (pictures) [Re: greenery1]
    #19081904 - 11/03/13 06:33 PM (10 years, 2 months ago)

Clear tubs that are 12"+ deep work best IMO


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: skippydude]
    #19082243 - 11/03/13 07:48 PM (10 years, 2 months ago)

Yeah once your subs in there's not much room for fruits


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Re: Is this Mycelium (or what) in LC? (pictures) [Re: cronicr]
    #19085703 - 11/04/13 02:35 PM (10 years, 2 months ago)

Alright thanks.


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Trays ready to fruit? [Re: greenery1]
    #19211163 - 12/01/13 03:44 AM (10 years, 1 month ago)

9 days crumbled from PF cake to coir/verm, just had one cake so made two small trays, how do they look? Ready for FC? Thanks.



Edited by greenery1 (12/01/13 01:06 PM)


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Re: Trays ready to fruit? [Re: greenery1]
    #19217752 - 12/02/13 03:17 PM (10 years, 1 month ago)

Will let that colonize more fully, then going to put my SGFC on the balcony, 68 at night to 86 during the day (this is winter now in bkk, but actually kinda chilly at night).


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trays pinning [Re: greenery1]
    #19223067 - 12/03/13 04:34 PM (10 years, 1 month ago)

A few pins just came up, going to put them in a SBFC tomorrow, question: the outside temp here in bangkok is about 68 at night and 86 during the day, I was going to put the FC on my balcony, should I make any adjustments to the FC due to the natural tropical environment?



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Re: trays pinning [Re: greenery1]
    #19223481 - 12/03/13 06:23 PM (10 years, 1 month ago)

You should fruit indoors with a SGFC at 70F-75F
86F is too high and will invite contamination


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Re: trays pinning [Re: skippydude]
    #19223518 - 12/03/13 06:29 PM (10 years, 1 month ago)

Quote:

skippydude said:
86F is too high and will invite contamination




Not outside its not. As it is it has a strong hold on the sub making it pretty contam resistant, and unlimited FAE is most contams worst nightmare :shrug: If OP's area has good enough RH, it will probably not even need a chamber, maybe some periodic heavy misting.


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Re: trays pinning [Re: Pastywhyte]
    #19232178 - 12/05/13 04:57 PM (10 years, 1 month ago)

thankss, will try that, some updates:



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Re: trays pinning [Re: greenery1]
    #19238880 - 12/06/13 10:51 PM (10 years, 1 month ago)

is the myc on the stipe normal?



Edited by greenery1 (12/06/13 10:53 PM)


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Re: trays pinning [Re: greenery1]
    #19238955 - 12/06/13 11:12 PM (10 years, 1 month ago)

That's sometimes called fuzzy feet.  It's your mushies telling you that they need more fresh air.

It's alright tho, they will still grow.  Just maximize FAE and they will be growing under better conditions.


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Edited by DannyDGAF (12/06/13 11:13 PM)


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split cap [Re: DannyDGAF]
    #19242902 - 12/07/13 10:37 PM (10 years, 1 month ago)

Have one split cap, I'm guessing due to rapid growth? The substrate of that tray has a tinge of yellow to it, contam?

The other tray looks to me that they are a bit short, still growing but the caps seem to be open prematurely, or is this normal?

How would you know if the sub is dry? Its shrinking here due to myc growth but not sure if its a bit dry.




Edited by greenery1 (12/07/13 10:50 PM)


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Re: split cap [Re: greenery1]
    #19243277 - 12/08/13 12:44 AM (10 years, 1 month ago)

Those looks bad mon :eek:

The tray with the split cap looks like it's excreting a lot of metabolites.
That is usually a sign of a contamination. 
Your spawn glasses looked pretty normal from your colonization pics,
but all these trays look like they have some pretty bad bacterial juju going on to me.

They look like they're in a FC on top of perlite, is that right?
I read somewhere you were thinking about putting them outside.

I'd start pointing my finger at the LC, but let's see what some other people have to say.

:goodluck:


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Re: split cap [Re: PirateSwazey]
    #19243761 - 12/08/13 06:01 AM (10 years, 1 month ago)

the iphone cam seems to make colors more vivid then they really are, not sure whats going on with the split cap tray, and the other tray looks like the gills are opening and they have finished growing.


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Re: split cap [Re: greenery1]
    #19244189 - 12/08/13 09:50 AM (10 years, 1 month ago)

Put a drop of that LC on agar
If it's bacterial it will get ugly quick

IMO the bacteria got on board when you fruited at 86F
You really should fruit SGFCs inside at 70F-75F


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Re: split cap [Re: skippydude]
    #19245195 - 12/08/13 02:03 PM (10 years, 1 month ago)

LC for this one already went off, tossed it, but I have 23 glasses standing by from a different LC, going to make mono's out of them. These two trays in the pics are the only ones I will use the FC for.


Going to try Manure/straw for one and Verm/coff/coir for the other.


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Re: split cap [Re: greenery1]
    #19249708 - 12/09/13 11:39 AM (10 years, 1 month ago)

veils broke, cut and dried, the next round of glasses r almost ready, how do these look to eat?



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Re: split cap [Re: greenery1]
    #19278921 - 12/15/13 11:32 AM (10 years, 1 month ago)

well here's to the LC which started this thread, and a nice 2g first 'successful' trip!

On the come down I began over-layering utube music, combined with some of my own piano music, and had a sort of 'satori' - was instantly in a new space and totally absorbed by its beauty and odd pathos. I was having a great laugh reading peoples comments to some flaky 'zen' new age music - bamboo flues, synth pads, oh the works, really nicely done schlock - people would say the dumbest things, like it was the greatest music they had ever heard, etc...and then one lady's comment about it really struck me as very authentic and true, it didn't make me laugh, so I went to her profile and started watching her home videos with an over lay of the 'zen' lounge music, then turned on my own music, and voila! Transported, put on my recorder and found a whole new world there. I've heard lots of stuff like this which is great, but anyways this was new for my own work.


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