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Offlinehihihi1717
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Why not leave inoculated jars in the SAB?
    #18963847 - 10/11/13 12:50 PM (10 years, 4 months ago)

I know the standard pf tek is to remove the jars from the SAB and put them on a shelf or something where they'll get FAE, but why not leave them in a SAB with a 70% iso alcohol soaked rag for a few days so they stay as contaminate free as possible?


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OfflineMush4Brains
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Re: Why not leave inoculated jars in the SAB? [Re: hihihi1717]
    #18963853 - 10/11/13 12:52 PM (10 years, 4 months ago)

First of all, colonizing jars should not be getting FAE.  They should be getting Gas Exchange through the holes in your jar.

If you want to leave them in your SAB, go ahead, no alcohol needed.


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OfflineSagescruffy
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Re: Why not leave inoculated jars in the SAB? [Re: Mush4Brains]
    #18963859 - 10/11/13 12:53 PM (10 years, 4 months ago)

Because the dry verm layer is an effective filter.


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Offlinehihihi1717
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Re: Why not leave inoculated jars in the SAB? [Re: Mush4Brains]
    #18963861 - 10/11/13 12:53 PM (10 years, 4 months ago)

Does it help prevent contamination, though?

Ninja edit: Meant GE not FAE.


Edited by hihihi1717 (10/11/13 12:54 PM)


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OfflineMush4Brains
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Re: Why not leave inoculated jars in the SAB? [Re: hihihi1717]
    #18963868 - 10/11/13 12:55 PM (10 years, 4 months ago)

If you made the jars correctly, sagescruffy is right, your dry verm barrier is protecting your jars.  If you want to keep them in your SAB it could mean less contaminants have the opportunity to fall into your jars, but shouldn't make a difference.

If you're getting contaminated jars, it's more likely due to improper sterilization or a contaminated syringe.


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Offlinehihihi1717
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Re: Why not leave inoculated jars in the SAB? [Re: Mush4Brains]
    #18963875 - 10/11/13 12:57 PM (10 years, 4 months ago)

Okay, and on that note, does the black soot that appeared on top of my jars (when the needle that was just flame sterilized scrapes the side of the hole) cause any issues typically? Or is the dry verm going to protect it?


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OfflinePussyFart
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Re: Why not leave inoculated jars in the SAB? [Re: hihihi1717]
    #18963878 - 10/11/13 12:58 PM (10 years, 4 months ago)

Quote:

Sagescruffy said:
Because the dry verm layer is an effective filter.



Quote:

hihihi1717 said:
Does it help prevent contamination, though?



Yes, that is the definition of effective.


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Offlinehihihi1717
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Re: Why not leave inoculated jars in the SAB? [Re: PussyFart]
    #18963884 - 10/11/13 12:59 PM (10 years, 4 months ago)

Quote:

Notahacker420 said:
Quote:

Sagescruffy said:
Because the dry verm layer is an effective filter.



Quote:

hihihi1717 said:
Does it help prevent contamination, though?



Yes, that is the definition of effective.




I replied before Sagescruffy's post went through. No need to be condescending about it.


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OfflineMush4Brains
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Re: Why not leave inoculated jars in the SAB? [Re: hihihi1717]
    #18963886 - 10/11/13 01:00 PM (10 years, 4 months ago)

Soot is fine.


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Offlinehihihi1717
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Re: Why not leave inoculated jars in the SAB? [Re: Mush4Brains]
    #18963900 - 10/11/13 01:03 PM (10 years, 4 months ago)

Okay, thank you for your help! Hopefully I'll see some colonization soon!  :cheers:


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InvisibleFrankHorrigan
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Re: Why not leave inoculated jars in the SAB? [Re: hihihi1717]
    #18963914 - 10/11/13 01:06 PM (10 years, 4 months ago)

Quote:

hihihi1717 said:
I know the standard pf tek is to remove the jars from the SAB and put them on a shelf or something where they'll get FAE, but why not leave them in a SAB with a 70% iso alcohol soaked rag for a few days so they stay as contaminate free as possible?




I keep every single colonizing jar and petri as protected as possible from drafts as possible.

I keep my jars in regular plastic tubs with the lid on until they are fully colonized. 54 quart jars in a 35-gallon tub and zero problems with GE.

If you want to leave them in your SAB, go for it.

There are never too many precautions you can take when dealing with sterile media.

No matter how effective you think your filter is, nothing is perfect. Minimize the risk :wink:


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Offlinehihihi1717
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Re: Why not leave inoculated jars in the SAB? [Re: FrankHorrigan]
    #18963936 - 10/11/13 01:12 PM (10 years, 4 months ago)

Quote:

FrankHorrigan said:
Quote:

hihihi1717 said:
I know the standard pf tek is to remove the jars from the SAB and put them on a shelf or something where they'll get FAE, but why not leave them in a SAB with a 70% iso alcohol soaked rag for a few days so they stay as contaminate free as possible?




I keep every single colonizing jar and petri as protected as possible from drafts as possible.

I keep my jars in regular plastic tubs with the lid on until they are fully colonized. 54 quart jars in a 35-gallon tub and zero problems with GE.

If you want to leave them in your SAB, go for it.

There are never too many precautions you can take when dealing with sterile media.

No matter how effective you think your filter is, nothing is perfect. Minimize the risk :wink:




Definitely, I left my 70% iso alcohol soaked rag in the SAB over night with the jars, too. Flame sterilized after every jar (which sucks because I feel like I'm killing too many spores that are hanging out in the needle). Even made sleeves for the box out of garbage bags. I'll be a sad panda if I get more than a couple contams :/


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OfflinePussyFart
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Re: Why not leave inoculated jars in the SAB? [Re: hihihi1717]
    #18963945 - 10/11/13 01:14 PM (10 years, 4 months ago)

Quote:

hihihi1717 said:
Flame sterilized after every jar (which sucks because I feel like I'm killing too many spores that are hanging out in the needle).



You're not, chill out.


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OfflineSockadin
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Re: Why not leave inoculated jars in the SAB? [Re: PussyFart]
    #18964165 - 10/11/13 01:59 PM (10 years, 4 months ago)

Quote:

Notahacker420 said:
Quote:

Sagescruffy said:
Because the dry verm layer is an effective filter.



Quote:

hihihi1717 said:
Does it help prevent contamination, though?



Yes, that is the definition of effective.





Made my day! :aweohyou:


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OfflineSagescruffy
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Re: Why not leave inoculated jars in the SAB? [Re: Sockadin]
    #18964181 - 10/11/13 02:03 PM (10 years, 4 months ago)

When I made my post I saw that we posted within seconds of each other.


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