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Offlinejohn311
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Registered: 10/10/13
Posts: 10
Last seen: 10 years, 4 months
Building a small "lab" question
    #18961156 - 10/10/13 07:40 PM (10 years, 4 months ago)

I have an empty room of my home that I would like to put a plastic sort of green house type room inside of with a stainless steel table and positive pressure from a true heap pushing air into the room..I would go in in a full tyvek suite,gloves,etc etc...Does this sound like a good idea or a waste of time..plan was to carry my pc in and do everything else in there from spawning jars to fruiting tubs..any advice?Or jut flat out don't waste the money?
PS it says one post but I lost my password to my other shroomery account lol.


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Offlinejohn311
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Re: Building a small "lab" question [Re: john311]
    #18961191 - 10/10/13 07:50 PM (10 years, 4 months ago)



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OfflineDorfnob
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Re: Building a small "lab" question [Re: john311]
    #18961219 - 10/10/13 07:59 PM (10 years, 4 months ago)

I'm not an expert but I'd say it could be a waste of time for a few reasons. First off if your going as far as putting in a lab room I'm assuming your using tyvek for the lids so idt any contams will get in if you leave them out in the open so to speak to colonize. you dont need a huge room to noc jars up in just make a still air box way, cheaper and less time consuming, another reason idk how accurate this is but what happens if your colonizing tubs and it comes down with the green meanie now your sealed room could be seriously contAmed. It's not a bad idea if you have the extra room and money but if you do it have a second seAled area for the fruiting. The air filter thing would be nice but I believe with proper procedure and cleanliness most contamination could be avoided. But hey if you do put in a lab it's going to look B.A.


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Offlinejohn311
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Registered: 10/10/13
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Last seen: 10 years, 4 months
Re: Building a small "lab" question [Re: Dorfnob]
    #18961246 - 10/10/13 08:08 PM (10 years, 4 months ago)

I feel like if the air was clean around the tubs from the hepa and everything was brought into that room inside my PC and then bleached, I wouldn't get contams.im pretty sure all my contams come from the dirty air.


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Offlinejohn311
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Posts: 10
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Re: Building a small "lab" question [Re: john311]
    #18961255 - 10/10/13 08:12 PM (10 years, 4 months ago)

I just have like 700$  to spend on making contams not happen...that was my best solution. Lol I'd love expert advice.


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OfflineDorfnob
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Re: Building a small "lab" question [Re: john311]
    #18961294 - 10/10/13 08:25 PM (10 years, 4 months ago)

It'd definitely cut back on the contams but what kind of bulk sub recipe do you use? Cuz if you use manure I'm pretty sure that has contaminates that survive pasturization.


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Offlinejohn311
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Registered: 10/10/13
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Re: Building a small "lab" question [Re: Dorfnob]
    #18961370 - 10/10/13 08:46 PM (10 years, 4 months ago)

Wbs jars to Cori/germ substrate


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Offlineg3n3h4x0r
Fungal Geneticist

Registered: 09/29/13
Posts: 73
Last seen: 8 years, 10 months
Re: Building a small "lab" question [Re: john311]
    #18961661 - 10/10/13 09:52 PM (10 years, 4 months ago)

I have my own genetics lab in my apartment that I'm waiting to make more permanent once I get a better place.  There are some things that are necessary to keep my things clean and other that are just there because I like them that way.  I do go to greater lengths to keep things sterile within my lab, and especially during the early stages of preparing mushrooms.

Most people would consider such an effort too extreme, but if it's something you'd like to do, I'd say go for it.  I create my own fume hood sort of thing to do bacterial cloning in, use gloves, sterilize everything, etc. when in most genetics labs people don't even use gloves and just do it on a tabletop.  The fact of the matter is, a bad result costs me more resources to figure out, so it makes more sense (for the use of my more valuable time) to go to the greater lengths. 

If you feel the same way, I'd do it.  Again, people are getting great results just being careful in the normal air of their own home, so it's not necessary.  It could be pretty cool, though.

If you do, give us some pics!


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Offlinedrake89
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Re: Building a small "lab" question [Re: g3n3h4x0r]
    #18963559 - 10/11/13 11:38 AM (10 years, 4 months ago)

well some people seem to have success in open air, but I do not know how.

for $700 you could buy, i mean build from new parts, a 24x24" flowhood.  get a stainless table, keep your room clean and you should be good.


--------------------
Fiery Fungi (like us on faeboo)


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Offlinejohn311
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Re: Building a small "lab" question [Re: drake89]
    #18963910 - 10/11/13 01:06 PM (10 years, 4 months ago)

I thought about a flow hood, but I have two issues with that..I'm very unsure of how to achieve laminar flow with the right fan/filter...and if I had a heap setup rated at 99.9 for a 400sf room inside a 100 sf room wouldn't that be just as clean as a flow hood but the whole room?


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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: Building a small "lab" question [Re: john311]
    #18972276 - 10/13/13 01:58 PM (10 years, 4 months ago)

Quote:

john311 said:
I thought about a flow hood, but I have two issues with that..I'm very unsure of how to achieve laminar flow with the right fan/filter...




Read up.

Quote:

and if I had a heap setup rated at 99.9 for a 400sf room inside a 100 sf room wouldn't that be just as clean as a flow hood but the whole room?




Not at all. You'd have to install expensive filters and blowers throughout the ceiling, as well as going to crazy lengths to keep things clean. And you'd still need a flowhood or SAB. For $700, buy a nice big AA PC and a flowhood.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Invisiblelipa

Registered: 07/24/07
Posts: 2,684
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Re: Building a small "lab" question [Re: drake89]
    #18973915 - 10/13/13 08:50 PM (10 years, 4 months ago)

Quote:

drake89 said:
well some people seem to have success in open air, but I do not know how.

for $700 you could buy, i mean build from new parts, a 24x24" flowhood.  get a stainless table, keep your room clean and you should be good.




This guys answer is the best one for 700$.  If you have a hood almost everything happens in front of it as far as culturing goes. Your technique will take care of the contamination issues.


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Offlinejohn311
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Registered: 10/10/13
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Re: Building a small "lab" question [Re: lipa]
    #18975316 - 10/14/13 05:16 AM (10 years, 4 months ago)

I started my flow hood build yesterday,i am building a 2x2 out of cabinet grade varnished plywood.ill look into some sort of cheapo heap for the whole room and keep it as clean as possible..I appreciate all the input.


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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: Building a small "lab" question [Re: john311]
    #18975982 - 10/14/13 10:30 AM (10 years, 4 months ago)

Quote:

john311 said:ill look into some sort of cheapo heap for the whole room




:facepalm:

No filters in the room! It only creates more air currents, which will only throw up contams that will get into your work. The ONLY filter you need is a flowhood.

If you have to have something in there, get one of those passive air filters.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Invisiblelipa

Registered: 07/24/07
Posts: 2,684
Trusted Cultivator
Re: Building a small "lab" question [Re: 36fuckin5]
    #18976200 - 10/14/13 11:35 AM (10 years, 4 months ago)

Quote:

36fuckin5 said:
Quote:

john311 said:ill look into some sort of cheapo heap for the whole room




:facepalm:

No filters in the room! It only creates more air currents, which will only throw up contams that will get into your work. The ONLY filter you need is a flowhood.

If you have to have something in there, get one of those passive air filters.




A whole room filter is very ideal for when the room is not being used. Especially if you live in an agricultural area with lots of dust. You can put it on a 15 min timer to stay on for a little while after the room is closed up. Set it near the entry way on the floor so it can scrub the lower part of the room.

Being as clean as possible is always a good thing and should never be frowned upon. It is part of having an organized lab space. I keep a filter on in the lab to reduce the amount of dust that seeps into the room through doors and windows.  I live in an equestrian area that is very dry and must take extra time to ensure dust does not follow me into my work area. Everyones situation is different but never is it too much to be too clean.


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: Building a small "lab" question [Re: lipa]
    #18976300 - 10/14/13 12:04 PM (10 years, 4 months ago)

Quote:

lipa said:
Quote:

36fuckin5 said:
Quote:

john311 said:ill look into some sort of cheapo heap for the whole room




:facepalm:

No filters in the room! It only creates more air currents, which will only throw up contams that will get into your work. The ONLY filter you need is a flowhood.

If you have to have something in there, get one of those passive air filters.




A whole room filter is very ideal for when the room is not being used. Especially if you live in an agricultural area with lots of dust. You can put it on a 15 min timer to stay on for a little while after the room is closed up. Set it near the entry way on the floor so it can scrub the lower part of the room.

Being as clean as possible is always a good thing and should never be frowned upon. It is part of having an organized lab space. I keep a filter on in the lab to reduce the amount of dust that seeps into the room through doors and windows.  I live in an equestrian area that is very dry and must take extra time to ensure dust does not follow me into my work area. Everyones situation is different but never is it too much to be too clean.




The issue, however, is circulating air currents. By the laws of physics, any air that you move will be replaced by dirty air. Unless you have an airlock and a serious clean room, a HEPA filter is counter-productive IME.

This is why I said to use a passive filter (such as an Ionic Breeze) instead of something with a blower.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineSexySmurff
HyperQube
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Registered: 06/21/04
Posts: 522
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Re: Building a small "lab" question [Re: 36fuckin5]
    #18976666 - 10/14/13 01:39 PM (10 years, 4 months ago)

running a hepa filter to reduce particulate load in the air is never harmful.

enclose the room, run the hepa and turn off when you want to perform inoculations. 

but don't grow in there.


--------------------
Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: Building a small "lab" question [Re: SexySmurff]
    #18977051 - 10/14/13 03:13 PM (10 years, 4 months ago)

Quote:

SexySmurff said:
running a hepa filter to reduce particulate load in the air is never harmful.




Except for when it circulates dirty air in from outside. Unless your room is sealed, this is GOING to happen.

Quote:

enclose the room, run the hepa and turn off when you want to perform inoculations. 

but don't grow in there.




Completely sealing a room is harder than you think.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineSexySmurff
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Registered: 06/21/04
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Re: Building a small "lab" question [Re: 36fuckin5]
    #18977154 - 10/14/13 03:31 PM (10 years, 4 months ago)

I know and ive prolly never achieved it.  But I always at least do this cover soft surfaces ie carpet and put up plastic sheeting to form an enclosure that  I must enter through a zipper.  Kind of in the vein of Dexter. And I do have a 24 x 18 flow hood


--------------------
Exalted is He from what they utter. Exalted is He from what they imagine. Exalted is He that exists without a place. Exalted is He that was there forever without a time or a beginning. Exalted is He that will be there forever without a time or an end. Exalted is He that will forever be praised. That is your God.


Edited by SexySmurff (10/14/13 03:42 PM)


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Invisiblefastfred
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Re: Building a small "lab" question [Re: SexySmurff]
    #18977249 - 10/14/13 03:49 PM (10 years, 4 months ago)

Nothing wrong with running a hepa in the room.

Most just run their flowhood for awhile.  It's exactly the same difference, except you can use a cheaper hepa and run your flowhood less.

If you're using a flowhood you've already given up on using any principle based on still air.

Personally, I can't believe the OMC is still hung up on laminar flow and hasn't learned to build proper enclosed flow hoods.  The amount of money wasted is staggering.


-FF


--------------------
It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy

The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed

"If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP)

I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid


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