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Mush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 3 months
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Eat them all 
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Blake_Shroom
Stranger



Registered: 09/09/09
Posts: 1,070
Last seen: 1 year, 3 months
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9.5 oz.... my new personal record
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highc
creator



Registered: 08/31/07
Posts: 3,592
Loc: maryland
Last seen: 7 years, 3 months
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Sexayy
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Blake_Shroom
Stranger



Registered: 09/09/09
Posts: 1,070
Last seen: 1 year, 3 months
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Re: 20 Tub Isolate grow [Re: highc]
#18958425 - 10/10/13 09:20 AM (10 years, 4 months ago) |
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A lot of the other tubs are pinning, but it isnt picture worthy yet. Maybe tomorrow they'll be looking real good like
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Brain Fart
Mushroom Nerd



Registered: 12/19/07
Posts: 2,538
Loc: Your Mom
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Sir, thank you for this. It is motivating me to stop being lazy lol
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Blake_Shroom
Stranger



Registered: 09/09/09
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Last seen: 1 year, 3 months
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Quote:
Blake_Shroom said:

9.5 oz.... my new personal record 
This whole tub was sterile... Any idea why that happened? Same culture for all these tubs. This one "stalled" for a bit when it was pinning. As you can see from my posts, my other tubs were well ahead of this one, even though they pinned 1-2 days apart.
I'm just worried that they wont be potent. I truly dont want to test them myself, i already planned an acid trip for today. i'll give some to some friends and see what they think 
Total yield so far off of 4 tubs and 1 bucket: 34 oz dried 
all tubs that were picked were put outside since its going to rain for like the next 2 weeks. hopefully i pull an oz off of each spent tub!
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Mush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 3 months
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Not sure why they came out sterile, but I would clone and make a master from a sterile fruit.
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Blake_Shroom
Stranger



Registered: 09/09/09
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Last seen: 1 year, 3 months
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Well they are already dry. I would have loved to have sterile fruits. I think if I cloned and tried to grow out again that the culture would be way too old
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Are you sure this is an isolate?
The way your tubs are varying so much between each (esp with the sterile tub) I would think your isolate my actually be a close-isolate but not quite.
Quote:
Mush4Brains said: Not sure why they came out sterile, but I would clone and make a master from a sterile fruit.
Not if it were an isolate, he'd have the same genetics he started with
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Oeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon
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Could be a spontaneous mutation. Something triggering that whole mycelial body to bypass spore production or something along those lines. Sporeless mushrooms are neat. Especially in species where spores are a health hazard, like oyster
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Blake_Shroom
Stranger



Registered: 09/09/09
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Last seen: 1 year, 3 months
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All of my other tubs + these 4 have looked the same, minus the one with the bigger ones. The sterile one is weird, I think i did something to the environment and it triggered it to not produce spores.
There is also a good chance I mislabled some petris
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Fair enough, I was just curious.
Regardless of petri labels being correct or not, you did a whole lot right here!
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Mush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 3 months
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Quote:
FrankHorrigan said: Are you sure this is an isolate?
The way your tubs are varying so much between each (esp with the sterile tub) I would think your isolate my actually be a close-isolate but not quite.
Quote:
Mush4Brains said: Not sure why they came out sterile, but I would clone and make a master from a sterile fruit.
Not if it were an isolate, he'd have the same genetics he started with 
Yes, but if it were a genetic mutation that arose then the clone will have the mutation and you can propagate it further.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Makes sense 
I've just never seen a spontaneous mutation in my isolates I've been working with for quite some time
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Mush4Brains
LOOL HACKED!!!

Registered: 07/31/13
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I don't doubt that you haven't, something like that to affect the whole tub would have to occur early in the spawn run, I would think. Could it be from physical conditions? I really have no clue, I mean, I took a few genetics courses during college, but I'm no expert (especially when talking about mushroom genetics). If it was an environmental factor, it won't be cloneable, but if it was a genetic mutation that came from a true isolate, it can be cloned I believe.
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Blake_Shroom
Stranger



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Mush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 3 months
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Do you get a lot of bottom pinning without using a liner? Doesn't look like that bucket turned out as good as your first one .
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Blake_Shroom
Stranger



Registered: 09/09/09
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Last seen: 1 year, 3 months
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Nope. I actually very rarely get bottom pins. Now side pins, that's a different story. I get a good amount of those. But it works good for me. If there are Amy bottom pins, they never mature until I toss the substrate outside and upside down.
I do have a tub with a liner going. Theres no pics of it because it hasn't even started knotting up yet. I think there's a bacteria issue with it. I may just toss it outside since its going to be rainy and humid for the next week or 2
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Blake_Shroom
Stranger



Registered: 09/09/09
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The 2 buckets produced 1.5 oz each, not bad. I've picked this tub. Its the only one that is different shaped, and it has consistently yielded higher, although its 56 qrt instead of 66 like all my others.

I'll post yields tonight
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Kiya_Star427
Live FREE or be a SLAVE!


Registered: 10/14/13
Posts: 355
Loc: New York
Last seen: 5 years, 2 months
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Cool so you didn't grow these on cakes? cuz it looks lie the whole bottom is just myocil
Edited by Kiya_Star427 (10/15/13 02:33 PM)
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