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Invisiblethakryptonian
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Registered: 09/03/13
Posts: 10
Metabolites "myc [sorry RR] Piss" questions
    #18962579 - 10/11/13 04:17 AM (10 years, 4 months ago)

Hey everyone i had a questions about metabolites.

If a cake has a decent amount of metabolites would it still be able to fruit?
Can metabolites enriched areas be removed ?
Any harm handling them?

I understand that quite often incubating at high temps can cause bacteria to flourish and metabolites are the defence mechanism for myc . will the Myc eventually beat the contam or are the metabolites a red flag to dispose.

Ive searched alot on this site and could not find any information on my specific questions.

Simply hypothetical no pics sorry


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Invisibletbagtag
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: thakryptonian]
    #18962651 - 10/11/13 05:26 AM (10 years, 4 months ago)

Metabolites are not always a sign of bacteria. Sometimes it's just from the mycelium being stressed.


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Invisiblethakryptonian
swim


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Re: Metabolites "myc [sorry RR] Piss" questions [Re: tbagtag]
    #18969005 - 10/12/13 04:32 PM (10 years, 4 months ago)

yea, but will the myc eventually overcome the stress and go back to healthy white?

also im still wondering

If a cake has a decent amount of metabolites would it still be able to fruit?
Can metabolites enriched areas be removed ?
Any harm handling them?


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OfflineStromriderM
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: thakryptonian]
    #18969054 - 10/12/13 04:48 PM (10 years, 4 months ago)

If you have a lot of metabolites especially early on in the colonization phase then it is probably a bacteria contamination


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InvisibleSpitballJedi
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: thakryptonian]
    #18969101 - 10/12/13 05:02 PM (10 years, 4 months ago)

Quote:

thakryptonian said:
yea, but will the myc eventually overcome the stress and go back to healthy white?




Does your myc understand the principles of present moment awareness? If not, then you will have remove the stress factors.

Pics would help


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Offlinekrypto2000
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: SpitballJedi]
    #18969147 - 10/12/13 05:19 PM (10 years, 4 months ago)

They're not dangerous to handle, just don't go drinking them. What do you mean by 'remove the problem areas' or however you put it? If it's causes by stress just pour the metabolites out when you birth them if they're pf-cakes or if it's bulk grain either pour them out before breaking up the grain to spawn or simply leave the metabolites in there and mix it into the substrate. If it's caused by bacteria depending what you're doing you *might* be able to remove he bacteria spot, but typically you want to throw it away. If you're not sure either post pics and we can tell you or fruit it and see. If you're doing cakes bacteria spreading from one cake to another isn't a huge issue if it's just a small spot. If it starts to consume a big part of a cake then simply remove it from the fruiting chamber before it spreads and toss it out.


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Invisiblethakryptonian
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Registered: 09/03/13
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: krypto2000]
    #18969263 - 10/12/13 05:52 PM (10 years, 4 months ago)

Does your myc understand the principles of present moment awareness?

I dont know what that means?

krypto2000 I didn't know you could pour them out i thought it was part of the cake

no pics just hypothetical


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InvisibleSpitballJedi
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: thakryptonian]
    #18969306 - 10/12/13 06:05 PM (10 years, 4 months ago)

I was being a smart-ass.

But really, metabolites may show up for various reasons. A little is normal, a lot can mean there's a problem.

When and if you get to the point where you have them, come back with a pic of your set up so it can be analyzed.

Until then, use the search feature in the top right hand corner of your screen to research metabolites or myc piss.

This topic has been discussed a 10googol times.


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OfflineSchmendrick
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: SpitballJedi]
    #18969333 - 10/12/13 06:12 PM (10 years, 4 months ago)

Quote:

SpitballJedi said:
This topic has been discussed a 10googol times.




I have told you 10googol times never to exaggerate.


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InvisibleSpitballJedi
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: Schmendrick]
    #18969398 - 10/12/13 06:29 PM (10 years, 4 months ago)

:lol:


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Offlinekrypto2000
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: thakryptonian]
    #18969473 - 10/12/13 06:52 PM (10 years, 4 months ago)

Quote:

thakryptonian said:
Does your myc understand the principles of present moment awareness?

I dont know what that means?

krypto2000 I didn't know you could pour them out i thought it was part of the cake

no pics just hypothetical




Do you actually have metabolites showing up or are you asking a hypothetical? Most times with jars, at least in my experience, metabolites first start showing up as simply yellow liquid which pools in the bottom, looks exactly like piss, there's no particular spots I tend to notice that it comes from. If you mean like a concentrated, almost gel like, appearance on the outside of a grain or cluster of brf then that basically the same thing, just more concentrated, or it could be a contaminant.

If you're doing a dunk tek then you can wash them off, but again it doesn't really matter. Typically those ime kind of dissapear and get integrated into the mycilium network or they make their way down to the bottom to form myc piss by the time the spawn is fully colonized. If you're doing pf-cakes and you either have liquid or metabolites pooling in the bottom by the time they're ready for birth you should and more or less have to pour those out during the spawning process anyway.

The only time I think it really matters is when they're drowning in it which can slow the growth, but the only time it's really convenient to safely pour that off is with a bulk grow and typically if it's drowning in myc piss then it's likely producing so much because your substrate is already pretty contaminanted or at the very least oversaturated.


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OfflineSnktido
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: thakryptonian]
    #19234386 - 12/05/13 10:51 PM (10 years, 2 months ago)

So metabolites result from ?

1. trying to get a foothold, breaking down grain
      -RR said that contamination may not even be present.
2. partially colonized, trying to fight off invasion
3. mostly colonized, stressed, shaking/brusing.

correct me if I am wrong. This is what I get out of it.

How long after appearance of metabolites should I wait before I trash the substrate? One guy said that his metabolites turned white after a while.


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OfflineSnktido
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: Snktido]
    #19234410 - 12/05/13 10:57 PM (10 years, 2 months ago)

Great answer! High colonized temp!

-RR

A large amount of metabolites generally means a bit of bacteria in the jar. The metabolites are antibiotic secretions, so when they're in large amounts, it usually means something is up. High colonization temps will also lead to metabolite production, so remember to colonize jars at room temperature.

A jar with a good filter will contain any contaminants that are within, so don't toss it out. Don't use any jar with a lot of metabolites for grain to grain transfers, but if they colonize fine, they're good to use for spawning to bulk.
RR


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OfflineSnktido
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: Snktido]
    #19234425 - 12/05/13 11:01 PM (10 years, 2 months ago)

O yeah, lots of metabolites if your grain is not fully exfoliated.


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OfflinePussyFart
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: Snktido]
    #19234431 - 12/05/13 11:03 PM (10 years, 2 months ago)

Quote:

Snktido said:
O yeah, lots of metabolites if your grain is not fully exfoliated.



:lolwut:


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Offline2bittoker
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: Snktido]
    #19234490 - 12/05/13 11:22 PM (10 years, 2 months ago)

Quote:

O yeah, lots of metabolites if your grain is not fully exfoliated.




Exfoliation is a process that occurs in plants. Fungi are not plants


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“I slept and dreamt that life was joy. I awoke and saw that life was service. I acted and behold, service was joy.”
"Love does not claim possession, but gives freedom"
― Rabindranath Tagore

Stuff for New Growers
Where new growers should start: RogerRabbit's PF Tek video    How it Should and Shouldn't Look 
My Simplified Bulk Growing  My OJ Shroom Tek


Edited by 2bittoker (12/05/13 11:23 PM)


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OfflineDannyDGAF
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: 2bittoker]
    #19234495 - 12/05/13 11:24 PM (10 years, 2 months ago)

I always thought that's what females do to their skin o_O


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Offline2bittoker
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: DannyDGAF]
    #19234515 - 12/05/13 11:28 PM (10 years, 2 months ago)

Quote:

DannyDGAF said:
I always thought that's what females do to their skin o_O



As it refers to a process in anything even close to mushrooms, exfoliation just refers to the loss of leaves during a plants seasonal life cycle http://en.m.wikipedia.org/wiki/Exfoliation_(botany)


--------------------
“I slept and dreamt that life was joy. I awoke and saw that life was service. I acted and behold, service was joy.”
"Love does not claim possession, but gives freedom"
― Rabindranath Tagore

Stuff for New Growers
Where new growers should start: RogerRabbit's PF Tek video    How it Should and Shouldn't Look 
My Simplified Bulk Growing  My OJ Shroom Tek


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OfflineSnktido
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: Snktido]
    #19235408 - 12/06/13 07:26 AM (10 years, 2 months ago)

ok, term use imprecisely, I apologize. To my understanding if your grain did not fully absorb water and break its husk, then your mycelium will produce metabolites to break down that husk in order to access the nutrients inside. I believe that in the spawn stage mycelium does not depend heavily on the husk material and more on the stored nutrition inside. Cellulose and other compounds from the husk would play a more important role later in the fruiting stage.

This is my speculation and would need more experience to verify. It would be nice if someone with a bigger belt can help do so.

Exfoliation: to remove the surface of (a bone, the skin, etc.) in scales or laminae. I our case to break or remove the outer husk.


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OfflineSnktido
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Re: Metabolites "myc [sorry RR] Piss" questions [Re: Snktido]
    #19235440 - 12/06/13 07:42 AM (10 years, 2 months ago)

I made a bunch of grain tubes. Results showed that seeds (too dry) with intact husks showed no signs of activity. Grain tubes with broken husks (fully saturated) showed colonization in the top layer and some metabolites accumulated at the bottom. Metabolites disappear after full colonization.  Wet fluffy grain (over saturation) showed little, extremely slow growth.


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