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lonelyjew
Stranger

Registered: 05/02/13
Posts: 300
Last seen: 3 years, 1 month
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Re: Monotub help. [Re: PussyFart] 1
#18968137 - 10/12/13 12:31 PM (10 years, 3 months ago) |
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alright well im only gonna use aobut 1 cc per pint jar so then i won't shake
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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lonelyjew
Stranger

Registered: 05/02/13
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Last seen: 3 years, 1 month
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Also how should i sterilize the tub before putting the substrate/spawn in there? or is it even necessary?
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
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Quote:
lonelyjew said: Also how should i sterilize the tub before putting the substrate/spawn in there? or is it even necessary?
It is not necessary. You can wipe it out with bleach or lysol if you want but you don't have to
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lonelyjew
Stranger

Registered: 05/02/13
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Last seen: 3 years, 1 month
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alright thanks.
http://www.shroomery.org/11402/Fast-easy-fool-proof-inoculation
this is the tek im using for inoculation do I still flame sterilize between injections?
also anyone have any good teks for homemade alcohol flame?
serious though thanks for all the replies this place is awesome!!
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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BittrBuffalo
Deaconica

Registered: 05/19/13
Posts: 1,729
Loc: Church of the SubGenus
Last seen: 3 years, 4 months
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I use that one with BRF jars and mycobags. I flame sterilize the needle before stabbing it into the sponge initially, then just pull the needle up into the sponge before moving to the next jar/hole without removing it from the sponge entirely until I'm finished inoculating. You can sterilize it between jars to be extra safe, but (gasp!) I don't see where it's absolutely necessary with the method I use. It's worked for me. Contams are a bummer, so that's why everyone seems like they freak out and treat it like they're handling vials of ebola or something. There are a million variables and EVERYTHING CAN GO TO SHIT AT ANY STAGE OF THE GAME HERE PEOPLE! It's important to remember that your entire life hinges upon this moment, and one wrong move and there will be nothing left of your entire grow but an enormous evil mushroom cloud (har har) that will one day kill us all.
ANYWAY...I don't know if there's such an animal as too much flame sterilization, but four jars of blue-green mold is a real downer. I have had enough success doing it my way that I'm satisfied with it. As always, YMMV. I apologize in advance if my antics may one day indeed bring upon the Earth an enormous death cloud that murders the planet.
-------------------- Disclaimer: This post is a work of fiction, provided for entertainment purposes only. Any resemblance to actual persons or events, past or present, is strictly coincidental. All celebrity voices are impersonated. If you begin your ID request with, "I just ate a bunch of these mushrooms…should I not have done that?" I'm just gonna sit back and watch Darwin at work.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Don't use that sponge. Flaming the needle red hot sterilizes it while wiping it with alcohol either on TP or in a sponge only sanitizes it. You want a sterile needle, don't wipe it in shit afterwards.
You don't need any special kind of flame, a lighter works great. If you worry about the soot try to put the needle at the bottom of the flame, where the fire is blue close to the metal.
IME this reduces soot if you've got a steady hand.
Good luck on your grow
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lonelyjew
Stranger

Registered: 05/02/13
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Last seen: 3 years, 1 month
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yeah it seemed like the sponge was overkill/ineffective
and thanks! as soon as i get my spores i will start a grow log!
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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BittrBuffalo
Deaconica

Registered: 05/19/13
Posts: 1,729
Loc: Church of the SubGenus
Last seen: 3 years, 4 months
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The idea is to be able to do without having to use a flowhood or glovebox. The alcohol-soaked sponge would ideally kill mold spores on your lid and protect your lid from mold spores in the air. Flaming the needle first would kill any nasties in or on the needle itself, and the sponge would protect it from mold in the air.
-------------------- Disclaimer: This post is a work of fiction, provided for entertainment purposes only. Any resemblance to actual persons or events, past or present, is strictly coincidental. All celebrity voices are impersonated. If you begin your ID request with, "I just ate a bunch of these mushrooms…should I not have done that?" I'm just gonna sit back and watch Darwin at work.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Quote:
BittrBuffalo said: The idea is to be able to do without having to use a flowhood or glovebox.
You are missing the still air box. Either way, it is poor practice to work with sterile media in open air, I don't care what you've seen, done, or read 
Quote:
The alcohol-soaked sponge would ideally kill mold spores on your lid and protect your lid from mold spores in the air. Flaming the needle first would kill any nasties in or on the needle itself, and the sponge would protect it from mold in the air.
Alcohol is not effective at neutralizing mold spores.
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lonelyjew
Stranger

Registered: 05/02/13
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so if i inoculate in a room that has been entirely scrubbed down with diluted bleach and has had an air purifier running constantly would i still get contams or is a still air box nessacary?
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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lonelyjew
Stranger

Registered: 05/02/13
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Last seen: 3 years, 1 month
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its also a pretty small room if that matters.
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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A cheap hepa air purifier will not scrub the air like you think it will. Nor will spraying chemicals everywhere or rubbing everything with bleach.
Use the still air box. Get used to it. Research it. Love it.
It is the difference between 50% success and 95% success. I speak from personal experience as well as the assistance I've provided many others. There is no reason not to jump in.
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lonelyjew
Stranger

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Last seen: 3 years, 1 month
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well i guess i better get on that still air box.
besides i guess i would need it because i want to make prints anyway.
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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lonelyjew
Stranger

Registered: 05/02/13
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Last seen: 3 years, 1 month
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question about the SAB
Do i spray everything down with bleach water solution then put the jars and needles in there then i respray? what about sterilization of the needle how would i flame sterilize needle in the SAB or is it even necessary?
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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refried

Registered: 06/14/13
Posts: 3,675
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your flame should be outside on the counter. if you try to light a flame inside where you've sprayed alcohol you could get a fireball on you ass.
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BittrBuffalo
Deaconica

Registered: 05/19/13
Posts: 1,729
Loc: Church of the SubGenus
Last seen: 3 years, 4 months
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I was only explaining the logic behind the tek, not encouraging or discouraging its use, or attesting to the truthfulness of the premise. I've used it and it's worked. This is neither here nor there; I'm not necessarily advocating its use.
Does this make me bad at growing mushrooms? So far, no. Does it make me a bad human for admitting the fact that I've used it and it's worked, despite it being Not The Way You're Supposed To Do It? Probably.
That's it.
-------------------- Disclaimer: This post is a work of fiction, provided for entertainment purposes only. Any resemblance to actual persons or events, past or present, is strictly coincidental. All celebrity voices are impersonated. If you begin your ID request with, "I just ate a bunch of these mushrooms…should I not have done that?" I'm just gonna sit back and watch Darwin at work.
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BittrBuffalo
Deaconica

Registered: 05/19/13
Posts: 1,729
Loc: Church of the SubGenus
Last seen: 3 years, 4 months
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I think it's time we give something new a try...There's no "I" in "threesome", FrankHorrigan.
-------------------- Disclaimer: This post is a work of fiction, provided for entertainment purposes only. Any resemblance to actual persons or events, past or present, is strictly coincidental. All celebrity voices are impersonated. If you begin your ID request with, "I just ate a bunch of these mushrooms…should I not have done that?" I'm just gonna sit back and watch Darwin at work.
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Cb411
New grower



Registered: 08/03/13
Posts: 110
Loc: New York new york
Last seen: 8 years, 11 days
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You can just clean it with soap and water it works cuz the air inside is still. It dosnt need to be sprayed with bleach or Lysol or anything
-------------------- "Reality is a crutch for people who can't handle drugs. "
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lonelyjew
Stranger

Registered: 05/02/13
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Last seen: 3 years, 1 month
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Re: Monotub help. [Re: Cb411]
#18973224 - 10/13/13 05:41 PM (10 years, 3 months ago) |
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alright so i flame sterilize the needle then put it in the still airbox? do i still wipe down all the jars with alcohol/bleach solution?
-------------------- Everything I say is a lie, I pretend to do drugs so people will think I'm cool.
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Cb411
New grower



Registered: 08/03/13
Posts: 110
Loc: New York new york
Last seen: 8 years, 11 days
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Yeah
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