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OfflineNoobiesnack
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Registered: 10/11/09
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Does the ideal LC Recipe ever change?
    #18960311 - 10/10/13 04:41 PM (10 years, 3 months ago)

Trying to get into growing edibles and just knocked up some ryeberry spawn bags with stropharia.  Only a little LC left in the purchased syringe.  Can I use similar LC recipes that are used for cubensis (Sugar in the raw is what I want to try because it's available to me) with the rest of the edibles?  I don't have a flow hood and think using the last of my LC to start another LC would help me stretch out this species I purchased so that I can grow and return to this species over and over.  Does anyone have advice on building a 'gene bank' of sorts?  I know if I am going to grow a few strains I do not want to purchase the same 3 LC syringes offline over and over.


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Offlinedrake89
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Re: Does the ideal LC Recipe ever change? [Re: Noobiesnack]
    #18961019 - 10/10/13 07:13 PM (10 years, 3 months ago)

you can try...my luck has been minimal.  i would just suggest using proper injection and breather ports over large filter disks.  but i've been really good at growing yeast with LC...


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OfflineNoobiesnack
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Re: Does the ideal LC Recipe ever change? [Re: drake89]
    #18961106 - 10/10/13 07:30 PM (10 years, 3 months ago)

Quote:

drake89 said:
you can try...my luck has been minimal.  i would just suggest using proper injection and breather ports over large filter disks.  but i've been really good at growing yeast with LC...




do u mean for stropharia in general or other gourmet species in LC?  Why would it not work if you already have mycelium in liquid.


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Invisibleliamtheloser
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Re: Does the ideal LC Recipe ever change? [Re: Noobiesnack]
    #18961221 - 10/10/13 08:00 PM (10 years, 3 months ago)

Because liquid culture easy to contaminate.

I won't use lc anymore anyway.  Agar is much easier and faster.

If you drop a wedge of agar in a jar of grain,  you get growth so fast.  LC has always taken forever to colonize a jar of grain.  When I order LC syringe,  first thing I do is knock up a few petri dishes.  Then use those to knock up grain.

Edit: I don't have a flow hood either,  but haven't had a contamination yet!


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Edited by liamtheloser (10/10/13 08:01 PM)


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OfflineNoobiesnack
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Re: Does the ideal LC Recipe ever change? [Re: Noobiesnack]
    #18961378 - 10/10/13 08:48 PM (10 years, 3 months ago)

I have plenty of agar... but my SAB is very primitive (a tote with 2 holes cut in it) so most of my petris got contams when I tried.  It's such a useless SAB, that I just shot up my cakes in the open air a couple of weeks ago. I just lsyol'd the room, wore gloves+surg mask, and wiped everything with alcohol napkins.
  Should I order more petris and try again?  How is your SAB/Glove box set up? I'm really not confident in my SAB and I suck balls at building things. I need to get better at this though because I'm mainly interested in gourmet mushrooms and the sterile technique to do agar plates and g2g seems a lot higher than just inocculating cakes with MS syringes.


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Invisibleliamtheloser
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Re: Does the ideal LC Recipe ever change? [Re: Noobiesnack]
    #18962444 - 10/11/13 02:28 AM (10 years, 3 months ago)

Get more plates,  but get glass or polypropylene ones so you can premix agar and pc them.  My SAB is literally a 15 gallon unlidded tote on its side. No arm holes, no lysol. I just keep my work in the rear of it, limit exposure to air, never pass my hands over an open plate.

If you need help I can help you figure out agar.


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OfflineNoobiesnack
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Re: Does the ideal LC Recipe ever change? [Re: liamtheloser]
    #18964559 - 10/11/13 03:37 PM (10 years, 3 months ago)

Sounds good.  Do any shroomery sponsors sell the polypropylene petris?  I was going to buy the plastic ones from mycosupply, but if you can pre-mix and sterilize that sounds really awesome.

I guess I can try regular plastic ones because they come sterile too though?  I don't have to open them until I have my agar ready in the SAB.  I've done this once before but I think over half of them contaminated, I could probably do it more eloquently this time around as it's been a few years.


Edited by Noobiesnack (10/11/13 03:44 PM)


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Invisibleliamtheloser
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Re: Does the ideal LC Recipe ever change? [Re: Noobiesnack]
    #18964627 - 10/11/13 03:53 PM (10 years, 3 months ago)

None of the sponsors sell autocalvable ones.  I bought a bunch of PP culture jars I use for tissue cultures as well. But I autoclave cheap little glass ones too,  they cost about a buck each on ebay. When pouring hot agar into a dish in a still air box,  a lot of air turbulence is created from the heat rising off of the plates. That's why I premix and then pressure cook my plates.


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OfflineNoobiesnack
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Re: Does the ideal LC Recipe ever change? [Re: liamtheloser]
    #18964659 - 10/11/13 03:58 PM (10 years, 3 months ago)

Quote:

liamtheloser said:
None of the sponsors sell autocalvable ones.  I bought a bunch of PP culture jars I use for tissue cultures as well. But I autoclave cheap little glass ones too,  they cost about a buck each on ebay. When pouring hot agar into a dish in a still air box,  a lot of air turbulence is created from the heat rising off of the plates. That's why I premix and then pressure cook my plates.




Can you post a picture of the PP Culture jars?  You are saying that you use small glass jars and PP jars and sterilize them with agar in the base so u don't have to pour petri dishes? I bet that works good, unless I misunderstand what you are doing.


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Invisibleliamtheloser
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Re: Does the ideal LC Recipe ever change? [Re: Noobiesnack]
    #18964683 - 10/11/13 04:04 PM (10 years, 3 months ago)

Yeah,  I'll show you a picture.

It does work very nice.  I have to wait til I get home to the a picture.


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OfflineHumbled
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Re: Does the ideal LC Recipe ever change? [Re: liamtheloser]
    #18964747 - 10/11/13 04:23 PM (10 years, 3 months ago)

I use presterilized petris in a sleeve and pour hot agar into them but I agree with liamtheloser about pre pouring then sterilizing. It does seem to work better when using a still air box.


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Invisibleliamtheloser
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Re: Does the ideal LC Recipe ever change? [Re: liamtheloser]
    #18964809 - 10/11/13 04:41 PM (10 years, 3 months ago)

You can pick up small PP Petris at tedpella.com for really cheap.  I got the culture jars from a seminar,  they're like 50 cents each.

I use micropore tape to close my Petris and cultures, it much cheaper than parafilm


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Invisibleliamtheloser
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Re: Does the ideal LC Recipe ever change? [Re: liamtheloser]
    #18965772 - 10/11/13 08:48 PM (10 years, 3 months ago)

here are photos of the PP culture jars:





And here are PP test tubes that I use for making slants:


Here are cheap glass petri dishes, i think they're 60mm:


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