|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
|
Question on taking prints
#18958344 - 10/10/13 08:45 AM (10 years, 3 months ago) |
|
|
Would it be best to take a print by picking a cap where the veil has yet to break and then allowing it to break or doing it yourself insideof the SAB or should the contaminant airborne spores not be an issue? Is there anything you try to do to minimize them besides taking it in a SAB or are a few acceptable since the mushies will outrace the few that will be there?
Oh, one more thing. Every syringe I've made after a day or so the spores always seem to settle against the wall of the syringe and shaking it doesn't seem to even do anything. How do you make them cluster and float in the middle like everything I've gotten from sponsors? Is it my water, or an additive in theirs maybe?
Edited by krypto2000 (10/10/13 08:48 AM)
|
hgmstl
Dough boy



Registered: 10/07/12
Posts: 463
Loc: The spot.
Last seen: 9 years, 10 months
|
Re: Question on taking prints [Re: krypto2000]
#18958366 - 10/10/13 08:53 AM (10 years, 3 months ago) |
|
|
You will want to wait for someone more experienced. But...
Quote:
krypto2000 said: Oh, one more thing. Every syringe I've made after a day or so the spores always seem to settle against the wall of the syringe and shaking it doesn't seem to even do anything. How do you make them cluster and float in the middle like everything I've gotten from sponsors? Is it my water, or an additive in theirs maybe?
My guess is your putting too many spores in each syringe.
I don't think you will have a lot of luck trying to print a cap that you picked before the veil breaks.
-------------------- Yo yo yo
|
bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Question on taking prints [Re: krypto2000]
#18958368 - 10/10/13 08:54 AM (10 years, 3 months ago) |
|
|
Quote:
krypto2000 said: Would it be best to take a print by picking a cap where the veil has yet to break and then allowing it to break or doing it yourself insideof the SAB or should the contaminant airborne spores not be an issue? Is there anything you try to do to minimize them besides taking it in a SAB or are a few acceptable since the mushies will outrace the few that will be there?
Oh, one more thing. Every syringe I've made after a day or so the spores always seem to settle against the wall of the syringe and shaking it doesn't seem to even do anything. How do you make them cluster and float in the middle like everything I've gotten from sponsors? Is it my water, or an additive in theirs maybe?
Just do it the way everyone else does. No one is having big problems doing it carefully with opened cap shrooms. Also if you don't want to see clumping add some jet dry or sex lube(like a drop). All of this is covered in RR's videos on spore printing.
|
SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,500
Loc: USA
Last seen: 4 seconds
|
Re: Question on taking prints [Re: hgmstl]
#18958393 - 10/10/13 09:06 AM (10 years, 3 months ago) |
|
|
Post a pic of your syringe because i cannot imagine how youre unable to break the spores up.
Krypto, sorry man but i just dont get it.. you have almost 5000 posts and have been a member since 2006. You should know this i would think.
The way i do my prints is after the veil breaks. I take the cap. Set in on aluminum foil in sab, let it drop a few spores. Then, move the cap to a wide mouth half pint jar and let it finish droping spores. Screw the lid on loosely. Recieve a nice print. Remove cap. Put the lid back on. Never had problems with my own prints.
Edited by SupaThaRipper (10/10/13 09:07 AM)
|
bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
|
Quote:
SupaThaRipper said: Post a pic of your syringe because i cannot imagine how youre unable to break the spores up.
Krypto, sorry man but i just dont get it.. you have almost 5000 posts and have been a member since 2006. You should know this i would. Think.
The way i do my prints is after the veil breaks. I take the cap. Set in on aluminum foil in sab, let it drop a few spores. Then, move the cap to a wide mouth half pint jar and let it finish droping spores. Screw the lid on loosely. Recieve a nice print. Remove cap. Put the lid back on. Never had problems with my own prints.
 he has been asking all sorts of weird questions and hypotheticals lately. If you just concentrated on growing shrooms the experience would show you that some of the questions you've been asking lately are dumb but I doubt you have the grows under your belt to see that.
http://www.shroomery.org/forums/dosearch.php?where=body&tosearch=main&namebox=166571&limit=25&sort=d&way=d&page=0 Half of these topics are 
Especially this gem http://www.shroomery.org/forums/showflat.php/Number/18931171#18931171
Quote:
FooMan said: I have to be honest, these questions are pretty damn strange bro, especially for someone who has been around as long as you. Please don't make the mods start keeping an eye on you.
Edited by Trusted cuItivator (10/10/13 09:29 AM)
|
SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,500
Loc: USA
Last seen: 4 seconds
|
Re: Question on taking prints [Re: bodhisatta]
#18958428 - 10/10/13 09:22 AM (10 years, 3 months ago) |
|
|
Lol i know it makes lul hard sometimes. I dont know if hes joking around half the time or not. Dont get me wrong though krypto, im not knocking you. Just dont understand you lol
|
krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
|
|
I've explained it before, but I used to have hardly any issues at all, but since moving I have been losing half of my grows to contams. My practices have only improved, yet it doesn't seem to get better, so I'm checking everything along the way. No matter how sterile I am it doesn't seem to make a difference. Also I'm not asking how to make the spores NOT clump, I've heard of the jetdry trick, I want them to clump. Mine just drift down onto the wall and stick to it shading it a dark purople/black and no amount of shaking gets them loose.
|
bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Question on taking prints [Re: krypto2000]
#18958502 - 10/10/13 09:55 AM (10 years, 3 months ago) |
|
|
Quote:
I used to have hardly any issues at all, but since moving I have been losing half of my grows to contams. My practices have only improved, yet it doesn't seem to get better
Then you were getting away with horrible procedure before and now that you're in a new place that demands proper procedure you're failing. Try doing things right 
Quote:
No matter how sterile I am it doesn't seem to make a difference.
You're missing something
Quote:
Also I'm not asking how to make the spores NOT clump, I've heard of the jetdry trick, I want them to clump. Mine just drift down onto the wall and stick to it shading it a dark purople/black and no amount of shaking gets them loose.
It's either jetdry/sex lube or nothing. They're going to stick or not stick. Unless you want to find another additive we don't know about.
|
krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
|
Re: Question on taking prints [Re: bodhisatta]
#18958602 - 10/10/13 10:21 AM (10 years, 3 months ago) |
|
|
Short of getting a flowhood or doing agar to ensure clean cultures (which I'm trying) I'm not sure what I can do to improve. I've cleaned the whole house (not specifically for mycology work), made a 'clean room' to isolate my grow to, I do everything in a SAB, wear gloves, facemask, clean everything, etc. I've tried underhydrating things in case it was too wet, it just doesn't seem to matter. Not that this place is dirty, but I feel like I'm trying to do sterile work in a landfill.
|
bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Question on taking prints [Re: krypto2000]
#18958626 - 10/10/13 10:30 AM (10 years, 3 months ago) |
|
|
A lot of people go for the overkill. Face mask, breathing mask, etc with their SAB and even alcohol spray. Sure a face mask and all that doesn't hurt (maybe the alcohol hurts actually). Thing is you can get 90+% success using just wrist length gloves, alcohol wipes for lids, and warm soapy mist in the SAB. I prefer to use a hard table as the bottom and the lid of the tote used for the SAB shot away. It's really your gestures and procedures. Keeping your hands from above sterile media, flaming and skillful use of the scalpel etc... Rather than all the precautions that are not the big issue. People try to avoid the big issue of keeping still sterile air and using quick choreographed movements because it's hard all the while trying to make up for it with everything else. Sure putting a full tyvek suit on and using a SAB inside of a tent is the best way to go aside from a laminar hood, but people have been getting away with a SAB on their kitchen table with the windows closed. I like to tape paper towel over the holes on my preped SAB for 20+ minutes before I go to use it to make sure the air inside has had time to drop out contams onto the slightly wet surfaces inside.
Do like 20 petri's at a time. Good practice and you'll probably end up with a few good ones.
Edited by Trusted cuItivator (10/10/13 10:31 AM)
|
krypto2000
Unknown


Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 3 months
|
Re: Question on taking prints [Re: krypto2000]
#18958849 - 10/10/13 11:19 AM (10 years, 3 months ago) |
|
|
That's pretty much what I do other than flipping the SAB upside down, and I don't use alcohol except on the injection ports. I do wipe the box with soapy water, wear a face mask, and gloves however. I just started a few MS petris so we'll see how they do. I still need practice pouring agar, but the ones I've done the past two times haven't contaminated at least (though one set was done just yesterday so I can't say much yet).
|
|