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InvisibleOneiricOutsider
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Registered: 10/05/13
Posts: 136
Loc: In the autumn shade
Re: Contams, contams and more contams. [Re: Pastywhyte]
    #18961243 - 10/10/13 08:07 PM (10 years, 3 months ago)

Ahh shit. Well follow the others' suggestions, although it seems like you're taking nearly every precaution. Perhaps try G2G/colonization in a different room with less air flow? Best of luck to you on your other jars, I think you'll get it this time :thumbup:


Edited by OneiricOutsider (10/10/13 08:08 PM)


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OfflineCitizenErased
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Registered: 05/10/13
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Last seen: 6 years, 11 months
Re: Contams, contams and more contams. [Re: OneiricOutsider]
    #18961251 - 10/10/13 08:11 PM (10 years, 3 months ago)

Do you think that it could in fact be the way that I've set up the holes in these buckets? Having two on the bottom, and four on top, rather than the other way around? Could it make that drastic of a difference?


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
Posts: 37,810
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Re: Contams, contams and more contams. [Re: CitizenErased]
    #18961273 - 10/10/13 08:17 PM (10 years, 3 months ago)

Personally I doubt it. Its not going to give very good conditions for fruiting and might make it easier for trich to move in later on down the road but, IME trich before the first flush is either bad spawn or bad pasteurization. Because your sub is just coir verm and gypsum, I'm tempted to blame the spawn.


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OfflineCitizenErased
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Re: Contams, contams and more contams. [Re: CitizenErased]
    #18961495 - 10/10/13 09:14 PM (10 years, 3 months ago)

I don't get it though. If they look and smell fine, how can I ever know whether they're good or not?


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OfflineCitizenErased
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Re: Contams, contams and more contams. [Re: CitizenErased]
    #18961506 - 10/10/13 09:16 PM (10 years, 3 months ago)

And which wouldn't give very good conditions for fruiting? 4/bottom,two/top or vice versa?


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InvisiblePastywhyteMDiscord
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Re: Contams, contams and more contams. [Re: CitizenErased]
    #18961670 - 10/10/13 09:54 PM (10 years, 3 months ago)

Quote:

CitizenErased said:
I don't get it though. If they look and smell fine, how can I ever know whether they're good or not?




IME molds in spawn can be buried in the jar's centre and if the contam is small you might not see or smell it. Best thing to do is ensure clean inoculate (agar) and good filters. Also check your sterilization times. I live at a fairly high elevation and when I was using a piece of shit PC that probably never got above 13psi I found that I needed to PC for 2-2.5 hours to be sure of clean grains.

Quote:

CitizenErased said:
And which wouldn't give very good conditions for fruiting? 4/bottom,two/top or vice versa?




Like I said a bucket is not something I have any experience fruiting in but in monotubs you want two loosely stuffed holes on the top and 4 tightly stuffed holes on bottom. No matter what, I think that you would want more holes on the bottom than the top. The loose poly will allow lots of FAE in, while the tightly stuffed holes provide the exhaust. Obviously less gas will exit through the tight holes which is why you would want more of those on the bottom :shrug:


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InvisibleMykes logos
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Registered: 08/05/12
Posts: 1,108
Loc: FL Flag
Re: Contams, contams and more contams. [Re: Pastywhyte]
    #18961707 - 10/10/13 10:03 PM (10 years, 3 months ago)

I think your issue is dirty spawn. To the untrained eye, molds and even bacterias may look just like mycelium.

Get some new genetics, some SFDs, and be sure your jars aren't open for more than 3 seconds while G2G'ing.

When spawning to tub or w/e, use a liner, and try the "layering" method rather than using your hands to mix.

Also, hold your breath during G2G transfers and while spawning myc to a tub.

I've grown a few different types of mushrooms, and the only times I had contamination, I was able to trace it back to earlier stages weeks before bad signs started showing up. It was usually bad inoculant/spawn.

Remember, growing mushrooms is just a race against time- you're just trying to get to that big flush before the mean green or w/e settles in.

Sterilize longer, become more sterile in your practices, and try again!

Good luck. Cheers.


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InvisiblePastywhyteMDiscord
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Re: Contams, contams and more contams. [Re: Mykes logos]
    #18961750 - 10/10/13 10:14 PM (10 years, 3 months ago)

Quote:

Mykes logos said:
Get some new genetics, some SFDs, and be sure your jars aren't open for more than 3 seconds while G2G'ing.




3 seconds is pretty short, I'd say he has five for sure :thumbup: I have noticed that its better to be slow and deliberate (causing little air disturbance in the SAB) than quick and jerky :shrug:

Quote:

Mykes logos said:
When spawning to tub or w/e, use a liner, and try the "layering" method rather than using your hands to mix.




I would never "layer" as it has been proven to take longer to colonize :shrug: I have also used my hands to mix. I don't even wear gloves *gasp* That's why we properly pasteurize in the first place :awesomenod:

Quote:

Mykes logos said:
Also, hold your breath during G2G transfers and while spawning myc to a tub.





So he can pass out with his arms in an SAB? :confused2: Just get a surgical mask. You could brush your teeth and use mouthwash as well if you were really paranoid. As for spawning you can pick your nose over a sub if its properly pasteurized :lol:


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OfflineCitizenErased
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Registered: 05/10/13
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Last seen: 6 years, 11 months
Re: Contams, contams and more contams. [Re: CitizenErased]
    #18962155 - 10/10/13 11:51 PM (10 years, 3 months ago)

I have a big box of surgical masks, that I dispose of after each use. And yeah, I shower, brush my teeth, etc.


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Re: Contams, contams and more contams. [Re: CitizenErased]
    #18962176 - 10/11/13 12:02 AM (10 years, 3 months ago)

Quote:

CitizenErased said:
And yeah, I shower, brush my teeth, etc.



Funny, I never do any of that before I do my G2G transfers, and they all turn out successful.

I just clean the immediate area, the SAB, and whatever goes inside.

Then I wear tyvek sleeves and surgical gloves, and there are sleeves attached to my SAB that my sleeved arms fit thru.

Works freakin awesome for me, might not for some....only advocated for the wickedly brave and confident.


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InvisibleSpitballJedi
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Registered: 10/13/12
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Re: Contams, contams and more contams. [Re: CitizenErased]
    #18962646 - 10/11/13 05:20 AM (10 years, 3 months ago)

Experiment with being more gentle when you mix in your spawn.

In my experience, I have a hard time accepting this super ninja contamination theory. It could be true, but I just don't completely buy in to it. I could be convinced otherwise, but it just don't sit right with me.


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