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OfflineCitizenErased
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Contams, contams and more contams.
    #18956922 - 10/09/13 10:20 PM (10 years, 3 months ago)

I really don't know what the deal is here. This is my third grow, the first of which being the only one to produce anything at all before contaminating, which was just over 1/2 oz dried. First I went with buckets, then tried a monotub, which went to shit fast. So I switched back to buckets for round three.

Rye berries, soaked for 24 hours, boiled for ten minutes, drained, shaken, dried (using the toilet paper test). Loaded into jars with polyfil in the center of the lids and foil over the tops. Pressure cooked at 15 psi for 90 minutes. Cooled overnight.

Still air box, cleaned thoroughly with soap and water, sprayed with lysol and wiped down with 90% iso for good measure("why not?", I thought). Then lathered lightly with soapy water again. Put everything inside, including master grain jar. Waited 15 minutes. Performed g2g slowly and carefully. 

Coir/verm/gypsum brought to field capacity, then pasteurized in jars, (keeping proper temps. 140-160 for an hour).

Smelled and examined all of my colonized rye. Found no problems. Dunked grains individually in jars, then mixed 2 jars of sub/1 jar of spawn individually among 7 buckets. Buckets have 1 inch holes on the bottoms just above the sub at the 4" mark on either side, and four holes of the same size around the top. Duct taped for colonization. Polyfil for fruiting. Stuffed as tightly as possible in the bottom holes, and as loosely as possible in the top holes. Showercaps on top, pointed down for colonization, and pulled up afterward. Electric fan on in room nearby but not directly on them. Temps at an average of 70-75 F. 

Waited 8 days to fruit for 4 of them, and 11 for three that didn't appear to be quite ready. Three days after putting the final three into fruiting, all but two buckets have trich, and I'm going to go ahead and assume the others most likely will by tomorrow.

What the shit am I doing so disastrously wrong?


Edited by CitizenErased (10/09/13 10:25 PM)


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InvisiblePastywhyteMDiscord
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Re: Contams, contams and more contams. [Re: CitizenErased]
    #18956949 - 10/09/13 10:27 PM (10 years, 3 months ago)

Could be your sterile procedure is not a good as you think, bad pasteurization (what kind of sub are you using), sub could be too wet, or else your inoculate is dirty.

Proper pasteurization and good field capacity for your substrate and agar for your inoculate will be your ticket :super:


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Offlinetwistedty
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Re: Contams, contams and more contams. [Re: Pastywhyte]
    #18957681 - 10/10/13 01:49 AM (10 years, 3 months ago)

bucket tek is like a lottery ticket my first five tubs i did with it did GREAT!

after that it was hit or miss. alot of misses than hits.

proper past in jars is the way to go.


FUCK THE BUCKET TEK. i find it sterilizes more than pastuerizes


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InvisiblebodhisattaMDiscordReddit
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Re: Contams, contams and more contams. [Re: twistedty]
    #18958352 - 10/10/13 08:50 AM (10 years, 3 months ago)

I don't think he is talking about the Damion5050 bucket tek but rather using a bucket as a fruiting chamber/monotub setup. He said he pasteurized his substrate in jars in the OP but some people can't read I guess.

Quote:

Coir/verm/gypsum brought to field capacity, then pasteurized in jars, (keeping proper temps. 140-160 for an hour).




My guess would be your sterile procedure with G2G transfers. Either that or the polyfil lids you have. Polyfill in a lid should be like a 1/4-3/8" hole and stuffed super tightly.


Edited by Trusted cuItivator (10/10/13 08:51 AM)


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InvisibleSpitballJedi
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Re: Contams, contams and more contams. [Re: bodhisatta]
    #18958758 - 10/10/13 10:59 AM (10 years, 3 months ago)

Could be the poly-fil. Could be the G2G. Could be the sterile process. Poly-fil or G2G are the more likely candidates IMO. I personally would never trust poly-fil for my grain jars.

I'm not exactly sure about your FC set up. In monotubs, we put 4 holes at substrate level and 2 holes at the top. It sounds like you have this reversed. Could be causing FAE issues that lead to contams. FAE is contams worst enemy.

And, just because it's green, doesn't mean it's trich.


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InvisiblePastywhyteMDiscord
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Re: Contams, contams and more contams. [Re: SpitballJedi]
    #18958885 - 10/10/13 11:26 AM (10 years, 3 months ago)

:whathesaid:


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OfflineCitizenErased
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Re: Contams, contams and more contams. [Re: Pastywhyte]
    #18959201 - 10/10/13 12:33 PM (10 years, 3 months ago)

Hm, so could I just modify my existing buckets rather than obtaining new ones? like if I were to drill two more holes at the bottom and simply tape the two extra at the top? Don't see why not, but just asking.


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InvisiblePastywhyteMDiscord
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Re: Contams, contams and more contams. [Re: CitizenErased]
    #18959348 - 10/10/13 01:12 PM (10 years, 3 months ago)

Probably but I don't fruit in buckets. Most people round these part prefer monotubs :shrug:


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InvisibleSpitballJedi
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Re: Contams, contams and more contams. [Re: Pastywhyte]
    #18959430 - 10/10/13 01:33 PM (10 years, 3 months ago)

:whathesaid:


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Offlinejimbojones
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Re: Contams, contams and more contams. [Re: SpitballJedi]
    #18959873 - 10/10/13 03:01 PM (10 years, 3 months ago)

I see three possible reasons for contamination here.

1. The jars you used for the gtg could have been contaminated.
  What was your initial inoculation procedure for the master grain jar?

2. If your cultures were clean, contams could have been introduced during the gtg transfers.  What exactly was your gtg procedure?

3. The polyfill filters could be letting contams in.


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InvisiblebodhisattaMDiscordReddit
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Re: Contams, contams and more contams. [Re: jimbojones]
    #18959885 - 10/10/13 03:04 PM (10 years, 3 months ago)

Quote:

jimbojones said:
I see three possible reasons for contamination here.

1. The jars you used for the gtg could have been contaminated.
  What was your initial inoculation procedure for the master grain jar?

2. If your cultures were clean, contams could have been introduced during the gtg transfers.  What exactly was your gtg procedure?

3. The polyfill filters could be letting contams in.




Thanks for the recap of what everyone already said. I think the OP will have an easier time reading this since you broke it down with numerals.


Edited by Trusted cuItivator (10/10/13 03:05 PM)


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InvisibleSpitballJedi
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Re: Contams, contams and more contams. [Re: bodhisatta]
    #18959902 - 10/10/13 03:08 PM (10 years, 3 months ago)

:rofl2:


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Offlinejimbojones
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Re: Contams, contams and more contams. [Re: bodhisatta]
    #18960276 - 10/10/13 04:31 PM (10 years, 3 months ago)

Quote:

bodhisatta said:

Thanks for the recap of what everyone already said. I think the OP will have an easier time reading this since you broke it down with numerals.




Yes, you're right I point out the same issues that everybody else did, but I'm the only one that actually asked questions about the OP's sterile technique. 

Sure you can tell somebody their sterile technique is to blame all day, but you gotta realize that they may have trouble understanding what correct sterile technique is.  This is why the specific steps of the OP's inoculation procedure of both the master and gtg's need to be looked at. Then we can all make suggestions on how exactly to rectify this contamination issue.


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InvisibleSpitballJedi
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Re: Contams, contams and more contams. [Re: jimbojones]
    #18960301 - 10/10/13 04:38 PM (10 years, 3 months ago)

You are a good customer service representative. I wished I got someone like you when I call in about my cable not working. Does the shroomery pay by the hour or are you salaried? I'm just a volunteer.


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InvisibleOneiricOutsider
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Re: Contams, contams and more contams. [Re: SpitballJedi]
    #18960402 - 10/10/13 04:58 PM (10 years, 3 months ago)

Lol it'd be awesome if the Shroomery paid some of their mods. OP, you sure it's trich? Pix worth a thousand...


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OfflineCitizenErased
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Re: Contams, contams and more contams. [Re: OneiricOutsider]
    #18960840 - 10/10/13 06:40 PM (10 years, 3 months ago)

I'll be able to take some pics within the hour. My master jar was inoculated g2g as well. I've been doing things this way exclusively since I had my first batch of spawn from MS. That is until the other day, when I used the rest of my left over spores (refrigerated) to inoculate 56 more jars. So...hopefully that works out.

The holes in my jars are around 1/4 inch in diameter. The polyfil is tightly fitted, and trimmed. My g2g is done inside of a still air box. I shower before hand, brush my teeth, put on clean clothes (that I spray with lysol for the hell of it). Then I put on an unused disposable showercap, and long sleeves with near-elbow length rubber gloves and a surgical mask (not a dust mask). I break up the master jar and put it inside of the clean box with the jars to be inoculated.

Then I wait 10 or 15 minutes for the air to settle and continue. I transfer the grains from the master by tilting and turning it so that they fall into the receiving jars, individually, and then close them up,as well as the master. I move on to the next jar and repeat. I do this as quickly, but with as little movement as I can manage to.


Edited by CitizenErased (10/10/13 06:43 PM)


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OfflineCitizenErased
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Re: Contams, contams and more contams. [Re: CitizenErased]
    #18960868 - 10/10/13 06:45 PM (10 years, 3 months ago)

Again, all of the spawn that I used for this grow looked and smelled fine. Plus, I mixed them individually into the buckets, thinking well if one jar is bad for some reason it won't fuck everything up. That's a big part of the reason I chose this route rather than to do a monotub, which I did end up doing, but that didn't go well at all. Shit turned black.


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OfflineCitizenErased
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Re: Contams, contams and more contams. [Re: OneiricOutsider]
    #18960998 - 10/10/13 07:07 PM (10 years, 3 months ago)













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OfflinePussyFart
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Re: Contams, contams and more contams. [Re: CitizenErased]
    #18961006 - 10/10/13 07:09 PM (10 years, 3 months ago)

:poopbanana:


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InvisiblePastywhyteMDiscord
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Re: Contams, contams and more contams. [Re: PussyFart]
    #18961096 - 10/10/13 07:28 PM (10 years, 3 months ago)

:whathesaid:


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