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Offlineoneloveoneloveonel
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BRF Cakes not pinning!?
    #18955420 - 10/09/13 05:12 PM (10 years, 4 months ago)

How's it going everybody; happy fall!



So last Saturday (five days ago), I dunked my four BRF cakes in distilled water and left them in the refrigerator for 24 hours.  After, I gave them a healthy coating of vermiculite and placed them in the fruiting chamber (duh!), and of course I let them sit for and hour or so until misting so the verm didn't wash away...

I've done my research and have found that it can take up to a week even two weeks for pinning to occur using the PF tek, however two of my cakes are on their second flush and seem to have ceased their magical growing abilities! 

The yield of these first two cakes seemed low (5 mushrooms total) compared to what I have seen here on shroomery; but I'm particularly concerned about how much longer they're taking to pin compared to their first flush!

Only one out of the four cakes have started fruiting since(a first flush cake)!  My roommate and I started a log for misting and fanning to keep up maintenance due to our schedules.



I am using a good five inches of perlite for humidification and fanning and misting AT LEAST 4+ times a day. These are Golden Teacher 1/2 pint cakes and yes this is my first time cultivating mushrooms.

Also, I would like to note that when I first inoculated the jars I only used around 1.5cc of the aqueous spore solution through one hole at the top of each of the jars.  I did this because, number one to conserve on the spore syringe, and number two I had researched that only shooting the jar once would reduce the probability of contamination...

...This did slow the growth of the mycelium however I did in fact wait a full week after each jar had become fully colonized before birthing.  But could this one inoculation point be the reason as to why my cakes have a rather low yield and delayed pinning!?

I hope all this information helps and thank you very much in advance.

Peace, one love.


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InvisibleTrentBoyett
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Re: BRF Cakes not pinning!? [Re: oneloveoneloveonel]
    #18955465 - 10/09/13 05:20 PM (10 years, 4 months ago)

Just be patient, sometimes they don't all pin at the same time, give it a few more days.

Are you fanning enough to evaporate the water from the cakes between mistings? Evaporation is a main pinning trigger.

Also is your SGFC elevated to allow airflow through the perlite(I can't tell from the pic)?

EDIT: Flushes can be weird, they're not always even, I wouldn't call five mushrooms a flush, that was just a few early bloomers.


Edited by TrentBoyett (10/09/13 05:23 PM)


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Offlinespacechildo
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Re: BRF Cakes not pinning!? [Re: oneloveoneloveonel]
    #18955469 - 10/09/13 05:21 PM (10 years, 4 months ago)

Your sgfc shows lots of condensation, which tells me you didn't build it according to specs.
1/4" holes in a GRID pattern 2" apart on all 6 sides.
every hole should then have 4 additional holes around them, 2" away.
Hence the word grid.

Not trying to be condescending, I just thought I'd be more precise in case you don't know what a grid pattern means :smile:

Take a picture of your whole setup. The sgfc must be elevated to work.


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Offlined9c6
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Re: BRF Cakes not pinning!? [Re: spacechildo]
    #18955498 - 10/09/13 05:27 PM (10 years, 4 months ago)

It took mine around 12 days to pin.


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InvisibleSpitballJedi
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Re: BRF Cakes not pinning!? [Re: spacechildo] * 1
    #18955518 - 10/09/13 05:31 PM (10 years, 4 months ago)

Sometimes it takes a while. Some cakes can be slower because of the variety of genetics in a MS syringe.

Hopefully the water I see on the walls of your SGFC is from spraying and not from condensation.

You don't need to mist on a particular schedule. Some people may need to mist more or less than others depending on your particular climate.

Spray cakes directly with a fine mist until they glisten, then fan a little. Once they no longer glisten, mist and fan again.

Once you start seeing how the cycle of misting/fanning is going, you can just about calculate when it needs it again. This knowledge will help you decide if you should mist/fan again before you leave the house.


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Offlinespacechildo
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Re: BRF Cakes not pinning!? [Re: SpitballJedi]
    #18955582 - 10/09/13 05:48 PM (10 years, 4 months ago)

Quote:

SpitballJedi said:
Sometimes it takes a while. Some cakes can be slower because of the variety of genetics in a MS syringe.

Hopefully the water I see on the walls of your SGFC is from spraying and not from condensation.

You don't need to mist on a particular schedule. Some people may need to mist more or less than others depending on your particular climate.

Spray cakes directly with a fine mist until they glisten, then fan a little. Once they no longer glisten, mist and fan again.

Once you start seeing how the cycle of misting/fanning is going, you can just about calculate when it needs it again. This knowledge will help you decide if you should mist/fan again before you leave the house.




Wouldn't the dry rings around his holes suggest this is condensation?
If the water on the walls had the time to dry up around his holes,
wouldn't it have had the time to dry up the rest too?


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InvisibleSpitballJedi
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Re: BRF Cakes not pinning!? [Re: spacechildo]
    #18955630 - 10/09/13 05:56 PM (10 years, 4 months ago)

Not necessarily. The farther you are from the source of incoming dry air, the slower the effect of drying.

If you spray the walls of your SGFC and put the lid on, it will take longer for the space between the holes to dry than the area directly around the holes.

So, it would depend on how heavily and long ago he sprayed and how much FAE he's getting.


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Offlinespacechildo
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Re: BRF Cakes not pinning!? [Re: SpitballJedi]
    #18955664 - 10/09/13 06:00 PM (10 years, 4 months ago)

Yes, that makes sense.
Upon further inspection, the rim of his sgfc is also dripping wet.
So I'll make an educated guess and say he relatively recently misted the walls,
but his sgfc still is not built to specs.
There should be more holes visible in the picture if he had them spaced 2" apart in a grid.. but idk :shrug:
Guess we'll have to wait for op's response :wink:


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Offlineoneloveoneloveonel
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Re: BRF Cakes not pinning!? [Re: spacechildo]
    #18955671 - 10/09/13 06:02 PM (10 years, 4 months ago)



This here is a picture of my terrarium from the outside. Yes, currently drinking some Octoberfest and drying out three shrooms (embarrasing) from the one cake that decided to fruit!.....

I screwed up with my first terrarium by drilling WAY too many holes in it. WAY TO MANY!! So I had to invest in a new fruiting chamber and this time I strategically placed 1/8inch holes about 1.75 inches give or take apart from each other in a grid formation. I do admit that this may be shy of RR recommendations only because I went drill happy with the first terrarium. How foolish of me.

Do you think I still need more holes?

Also foolish of me, I don't have it elevated above the ground. But yes I do in fact have holes in the bottom. I gotta fix that.

Yes, the condensation is from misting.

I will continue to wait it out for the other three cakes to pin but thanks for the awesome feedback my friends.

Peace, one love.


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Invisibleskuzzy1232
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Re: BRF Cakes not pinning!? [Re: oneloveoneloveonel]
    #18955686 - 10/09/13 06:05 PM (10 years, 4 months ago)

Humidity might actually be too low ...  you are fanning way to much sir , i suggest twice a day at the most .


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InvisibleTrentBoyett
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Re: BRF Cakes not pinning!? [Re: oneloveoneloveonel]
    #18955697 - 10/09/13 06:06 PM (10 years, 4 months ago)

There should be 1/4 holes every two inches on all six sides, so literally everywhere, even the top and bottom, and you have to elevate the fc off the table so air can come up through the holes on the bottom.


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InvisibleTrentBoyett
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Re: BRF Cakes not pinning!? [Re: skuzzy1232]
    #18955708 - 10/09/13 06:08 PM (10 years, 4 months ago)

Quote:

skuzzy1232 said:
Humidity might actually be too low ...  you are fanning way to much sir , i suggest twice a day at the most .




The humidity us probably too low because of the lack of holes allowing the air to flow through the perlite and get hydrated.


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InvisibleSpitballJedi
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Re: BRF Cakes not pinning!? [Re: oneloveoneloveonel]
    #18955720 - 10/09/13 06:09 PM (10 years, 4 months ago)

Your spacing is fine, but they really should be 1/4" holes to allow good airflow.

Also, having it sit on the table is like having no holes in the bottom. It has to be elevated so the fresh air can come in through the bottom and move up through the damp perlite. This air flow is how RH and FAE is maintained.


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Offlineoneloveoneloveonel
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Re: BRF Cakes not pinning!? [Re: oneloveoneloveonel]
    #18955753 - 10/09/13 06:17 PM (10 years, 4 months ago)

Sorry, I don't have a quarter inch drill bit.  How would drilling an additional ten or so holes on each side sound to catch up to par?!

One love.


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InvisibleSpitballJedi
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Re: BRF Cakes not pinning!? [Re: oneloveoneloveonel]
    #18955777 - 10/09/13 06:23 PM (10 years, 4 months ago)

I'm not trying to be a dick, but I really don't know if a different wrong way will work better. :shrug:

I'm a sheep and try to only do it the way I'm told.


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Invisibleskuzzy1232
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Re: BRF Cakes not pinning!? [Re: TrentBoyett]
    #18965913 - 10/11/13 09:21 PM (10 years, 4 months ago)

Could be but I've have grown fine cakes in a shot gun terrarium sitting on the ground not built to spec and maintained proper humidity. I have never seen a shotgun terrarium at a hydrometer reading of 100% not have condensation on the walls , I will say however that i think it is a collection of thing's there are more than one factor sir. I think the holes are fine and i think you might even want to tape up some of those holes on the sides to up the humidity RR gave me that advice once. fanning twice a day is optimal for a tub that size as far as FAE goes so it will be fine  its more than enough. I experimented a lot with mine before moving to mono tubs.


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Offlinespacechildo
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Re: BRF Cakes not pinning!? [Re: skuzzy1232]
    #18967045 - 10/12/13 03:49 AM (10 years, 4 months ago)

RR told you to tape up some holes on his sgfc design?
I highly doubt that..


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OfflinePussyFart
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Re: BRF Cakes not pinning!? [Re: skuzzy1232]
    #18967070 - 10/12/13 04:01 AM (10 years, 4 months ago)

Quote:

skuzzy1232 said:
I have never seen a shotgun terrarium at a hydrometer reading of 100% not have condensation on the walls



Well then you have not met the search function yet.

You can have 100% RH with 0 condensation if there is no temperature differential.

And in a properly built SGFC, there will be no temperature differential.

Quote:

skuzzy1232 said:
i think you might even want to tape up some of those holes on the sides to up the humidity RR gave me that advice once.



:callingbullshit:      Prove it.

Taping holes on a SGFC will only lower the humidity.


Edited by PussyFart (10/12/13 04:01 AM)


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