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Offlinekrypto2000
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Re: Check my SAB/Agar procedure and a couple questions on handling petris. [Re: SpitballJedi]
    #18958864 - 10/10/13 11:22 AM (10 years, 3 months ago)

By no pour jars you guys are referring to pcing them in half pints and using those instead of proper petris? I've thought about doing that, but it seems like a half measure. The only real benefit I see with it is ease of doing MS or liquid culture since you can inoculate them without opening them, but if you ever want to do isolation work I don't see the point, and if all you're doing is nocing up a petri to transfer to grain you might as well skip it and just do a g2g.


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InvisibleSpitballJedi
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Re: Check my SAB/Agar procedure and a couple questions on handling petris. [Re: krypto2000]
    #18959004 - 10/10/13 11:50 AM (10 years, 3 months ago)

A Jedi you will become, Pattawan.


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Offlinekrypto2000
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Re: Check my SAB/Agar procedure and a couple questions on handling petris. [Re: SpitballJedi]
    #18959056 - 10/10/13 12:04 PM (10 years, 3 months ago)

I hope so :mushroom2:


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InvisiblePitbullgrin
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Re: Check my SAB/Agar procedure and a couple questions on handling petris. [Re: krypto2000]
    #18961803 - 10/10/13 10:24 PM (10 years, 3 months ago)

10g agar 10g LME 500ml/2cups h2o.1/4 pint jars with plastic unfiltered lids PCd for 45 min. Used for print to tissues. Use what works for you. Agar is fun!! :headbang3:


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InvisiblebodhisattaMDiscordReddit
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Re: Check my SAB/Agar procedure and a couple questions on handling petris. [Re: Pitbullgrin]
    #18962802 - 10/11/13 07:09 AM (10 years, 3 months ago)

Quote:

Pitbullgrin said:
10g agar 10g LME 500ml/2cups h2o.1/4 pint jars with plastic unfiltered lids PCd for 45 min. Used for print to tissues. Use what works for you. Agar is fun!! :headbang3:




I always got mad condensation using those with metal filtered lids. Unless your talking the whole jar being plastic like the 1/4pt zip locks those implode without a gas exchange.


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Offlinekrypto2000
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Re: Check my SAB/Agar procedure and a couple questions on handling petris. [Re: bodhisatta]
    #18963003 - 10/11/13 08:47 AM (10 years, 3 months ago)

Is condensation bad other than it obscuring your view?


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InvisiblebodhisattaMDiscordReddit
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Re: Check my SAB/Agar procedure and a couple questions on handling petris. [Re: krypto2000]
    #18963020 - 10/11/13 08:57 AM (10 years, 3 months ago)

Yes it aids in bacterial infections. It makes myc growth happen everywhere if you use spores, which isn't good for sectoring and isolation. It makes isolation hard to do since the water carries everything across the agar's surface.

I had these problems on every no pour jar I ever made using 10g agar 10g ELME/LME and 500ml h2o


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Offlineskippydude
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Re: Check my SAB/Agar procedure and a couple questions on handling petris. [Re: bodhisatta]
    #18963102 - 10/11/13 09:25 AM (10 years, 3 months ago)

.5ml Gentamicin per 500ml agar would eliminate most of your bacteria problems, but condensation is still bad. Store your dishes where the temperature is stable to avoid it. Storing dishes upside down helps too. In my culture fridge the slants are kept in a thick Styrofoam box as a buffer against temperature change from opening the door.


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