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OfflineeLeSDenes
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Ps. galindoi isolation
    #18950258 - 10/08/13 04:05 PM (10 years, 4 months ago)

hello everyone,

I am trying to isolate a super sclerotia producer. According to RR, i should pick spots early which are brown. I was reading the posts a lot and i found out that browning occurs weeks later for most of the people. I do not have any brown spots either. So my question is that if I should just pick some nice growing spots as in normal isolation or should i wait for those brownies? I also heard that if i transfer the brown thing I have to wait weeks for that too to turn brown...
anyway i post a picture because that's what everyone likes :smile:



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InvisibleFrankHorrigan
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Registered: 01/04/11
Posts: 10,573
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Re: Ps. galindoi isolation [Re: eLeSDenes]
    #18950486 - 10/08/13 05:00 PM (10 years, 4 months ago)

Be patient. If that plate is contam-free like it appears to be, you should let it grow out.


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OfflineeLeSDenes
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Re: Ps. galindoi isolation [Re: FrankHorrigan]
    #18950527 - 10/08/13 05:07 PM (10 years, 4 months ago)

thanks Frank! I thought i should keep the mycelium fresh and young and make transfers as early as possible. I'll just wait for those spots then. i am going to upload more pictures soon with every transfer i make.


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InvisibleFrankHorrigan
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Re: Ps. galindoi isolation [Re: eLeSDenes]
    #18950535 - 10/08/13 05:08 PM (10 years, 4 months ago)

Nah, it won't hurt it so long as you keep it wrapped well.

I waited for my MS dish to start producing tiny stones on the agar. Then I transferred those and isolated a couple badass stone-makers myself.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineeLeSDenes
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Re: Ps. galindoi isolation [Re: FrankHorrigan]
    #18953152 - 10/09/13 07:46 AM (10 years, 4 months ago)

This is the answer i have been looking for. Thanks Frank you are genius!


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