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OfflineIcesyn
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Raw Sugar MS Liquid Culture w/o Pressure cooker
    #18934787 - 10/05/13 05:09 AM (10 years, 4 months ago)

Gonna do about four jars two at 2% sugar to water and the others at three and four. Steaming for about 6 hours. That should do it.
Fingers crossed

:winning1:


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OfflineeLeSDenes
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: Icesyn]
    #18934835 - 10/05/13 05:40 AM (10 years, 4 months ago)

I would not even try it without the pressure cooker and you probably end up with contams because your doing it from MS.


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OfflineIcesyn
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: eLeSDenes]
    #18934881 - 10/05/13 06:10 AM (10 years, 4 months ago)

You seem to lack some knowledge here man. Besides, where's your optimism?

Sterile LC solution and guaranteed sterile spores. Contam? Only one way to know for sure. Of course I have doubts. As I would doing this with any other approach. But I'm experienced enough to stand my ground and share these flexible experimental procedures with you guys. Don't be shy with an unfamiliar method. Do some research and come back with something solid to prove your opinion. I'm sure you wont.

Let's see what happens.


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OfflineeLeSDenes
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: Icesyn]
    #18934904 - 10/05/13 06:26 AM (10 years, 4 months ago)

I've done lc enough to know it is a waste of time if yo not doing it right. If you have sterile spores it could work though. But if you have some experience why do you bother with ms lc? Why don't you just grow some nice fruits and do a clone lc?


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OfflineIcesyn
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: eLeSDenes]
    #18934924 - 10/05/13 06:35 AM (10 years, 4 months ago)

I have no live specimens to work with. And this is perhaps the third or fourth time I'm trying MSLC in the passed couple years. First time without a PC. Yeah MSLC can sometimes be a pain. But I'm sure that's not always the case. Sterile solution, sterile spores, why wouldn't it work. The only issue I see is the fact I'm using MS. Justified by my means and has worked out for many others.

Now let's see some optimism.


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Invisiblemaddchef
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: Icesyn]
    #18935109 - 10/05/13 08:28 AM (10 years, 4 months ago)

Lmao @ sterile spores. Id love to hear where you got this notion.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineIcesyn
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: maddchef]
    #18935127 - 10/05/13 08:35 AM (10 years, 4 months ago)

You know what I mean!

From a trusted vendor. Haven't had an issue so I say "sterile" with trust that my chosen vendor had supplied me with a clean sample. Not necessarily sterile, but pretty damn clean.


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Invisiblemaddchef
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: Icesyn]
    #18935232 - 10/05/13 09:01 AM (10 years, 4 months ago)

But that's why most lc's fail. Fairly clean isn't good enough for an lc. Say you had very minimal contams, 2 foreign spores per half cc. Problem becomes putting those 2 spores into a highly nutritious medium as they begin to multiply their ass off. If you have the spores, just go to agar with them, grow out a jar and make a glc, or drop a colonized grain in (not recommended).


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineIcesyn
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: maddchef]
    #18935291 - 10/05/13 09:14 AM (10 years, 4 months ago)

I suppose that's how you play the game with MSLC.

And also why I said fingers crossed. I mean, what if all four jars grow nice and healthy? Wont know till I try. Worth a shot anyways. I'll try tissue cloning next. Let me enjoy my ridiculous baby steps lol.


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OfflineeLeSDenes
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: Icesyn]
    #18935420 - 10/05/13 09:50 AM (10 years, 4 months ago)

You can get sterile spores with invitro fruiting. But i doubt that vendors do that at all. They work on a commercial scale and although they work in very clean environment they must have some shit on the prints anyway. If it is so important for you to have ms lc then put spores on agar, do a transfer and make LC.  I know it is disappointing but many of us tried this already and you can save your time and spores for something better.


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OfflineIcesyn
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: eLeSDenes]
    #18935471 - 10/05/13 10:04 AM (10 years, 4 months ago)

No thanks lol. I understand what you mean but I have had successful MSLCs in the past and have to hold off on working with agar for a little while longer. I have plenty of spores and raw sugar. I want to spend some time buffering my cultures until I have something substantial to share here.

Any advice for reducing sediments? Gotta grab some bottled spring water before I whip up some sterile sugar water.


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OfflineStromriderM
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: Icesyn]
    #18935534 - 10/05/13 10:29 AM (10 years, 4 months ago)

All this nonsense is why I love my agar!


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OfflineIcesyn
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: Stromrider]
    #18935544 - 10/05/13 10:34 AM (10 years, 4 months ago)

Nonsense?


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OfflineRogerRabbitM
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: Icesyn] * 1
    #18935562 - 10/05/13 10:38 AM (10 years, 4 months ago)

I doubt raw sugar is going to work very well, but you're right about not needing a pressure cooker to sterilize it.  We use a sterilizer on grains because they harbor bacterial endospores.  Sugar does not, so steaming will work fine.  I used to make Karo LC all the time with a simple boil.  I'm no fan of LC, but just wanted to clear the sterilization part up.
RR


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OfflineIcesyn
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: RogerRabbit]
    #18935574 - 10/05/13 10:42 AM (10 years, 4 months ago)

Quote:

RogerRabbit said:
I doubt raw sugar is going to work very well, but you're right about not needing a pressure cooker to sterilize it.  We use a sterilizer on grains because they harbor bacterial endospores.  Sugar does not, so steaming will work fine.  I used to make Karo LC all the time with a simple boil.  I'm no fan of LC, but just wanted to clear the sterilization part up.
RR



Thanks RR.

I'd like to add that I have had quite a few grows with steam sterilized rye grain. All successful. Run of luck perhaps?


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OfflineRogerRabbitM
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: Icesyn]
    #18935586 - 10/05/13 10:47 AM (10 years, 4 months ago)

Me too.  It doesn't 'always' harbor endospores, but can.  This is why we use a pressure cooker.  I've also written a lot in the gourmet forum about steaming grains for 8 hours at near ambient pressure(1 psi) as we do on our farm.  We also sterilize our supplemented sawdust blocks for 8 hours at 1 psi using my homebuilt wood fired boiler.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineIcesyn
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: RogerRabbit]
    #18935614 - 10/05/13 10:52 AM (10 years, 4 months ago)

Interesting  :thatsinteresting:

Still using that big sterilizer you've made a while back? I was impressed when I stumbled through your gallery.

[Edit]
Oh didn't catch that. I suppose you are haha


Edited by Icesyn (10/05/13 10:54 AM)


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OfflineStromriderM
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: Stromrider]
    #18935635 - 10/05/13 10:58 AM (10 years, 4 months ago)

Quote:

psillyshroomer said:
All this nonsense is why I love my agar!




By nonsense I mean worrying about contamination and testing lc. With agar I know what I got and I love that piece of mind.

Using a clean culture from agar to inoculate a grain master and then doing g2g ttransfers is the most reliable and efficient way to increase culture stock imo


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OfflineIcesyn
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: Stromrider]
    #18935646 - 10/05/13 11:04 AM (10 years, 4 months ago)

Quote:

psillyshroomer said:
Using a clean culture from agar to inoculate a grain master and then doing g2g ttransfers is the most reliable and efficient way to increase culture stock imo




I believe it


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OfflineIcesyn
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Re: Raw Sugar MS Liquid Culture w/o Pressure cooker [Re: Icesyn]
    #18953074 - 10/09/13 07:11 AM (10 years, 4 months ago)

Starting to see something. A few little white puffy specs that weren't there before. Spores are germinating.

Pics in a week or two, testing each of them with PF tek probably by the end of the month.

Fingers crossed!


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Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Kratom Powder for Sale   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Grow Bags   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Capsules   North Spore Injection Grain Bag


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