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OnlineSupaThaRipper
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Pan cyan spore syringe?
    #18948377 - 10/08/13 06:52 AM (10 years, 3 months ago)

So I recieved a pan cyan rdu spore syringe from a reliable vendor(reliable by heard reputation.)
So far ive been pretty worried. I dont know what the deal is or if this is expected. I inoculated 7 rye berry spawn jars and 5 lme agar plates all in a sab. So far, no jars show any growth and 4 out of the 5 plates show absolutely no growth. One however is questionable... Is this mycelium or contaminate? Please ignore the brown flakes. I had some medical tape fall in the pot and burn.. said fuck it and used it anyways lol. Btw inoculated 10-1



I know the agar is fine because my galindoi and mexicana plates are turning out fine..


Ugh..
Is that decent looking pan cyan mycelium or just contam?
Is it possible I didnt shake the syringe enough MAYBE, consisering the red spore is hard to see..?
Honestly I dont know what else it could be other than a bad syringe..

Any ideas?


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OnlineSupaThaRipper
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Re: Pan cyan spore syringe? [Re: SupaThaRipper]
    #18948385 - 10/08/13 06:58 AM (10 years, 3 months ago)

Ps I am pretty new to agar.. my recipe is 500ml water, 10g lme, and 9g agar agar flakes. I got this recipe of Deathryders blog.

Oh and here are the spawn jars knocked 10-1


Edited by SupaThaRipper (10/08/13 06:58 AM)


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OfflineNakor420
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Re: Pan cyan spore syringe? [Re: SupaThaRipper]
    #18948394 - 10/08/13 07:04 AM (10 years, 3 months ago)

That culture does indeed look like Pan mycelium...though if I were you...I'd transfer out of that plate IMMEDIATELY.


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OnlineSupaThaRipper
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Re: Pan cyan spore syringe? [Re: Nakor420]
    #18948401 - 10/08/13 07:09 AM (10 years, 3 months ago)

Why immediately? Does it not look healthy?


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OfflineNakor420
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Re: Pan cyan spore syringe? [Re: SupaThaRipper]
    #18948406 - 10/08/13 07:14 AM (10 years, 3 months ago)

Well it says non sterile pour...and I see some dark spots all over the plate...what are those dark spots?


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OnlineSupaThaRipper
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Re: Pan cyan spore syringe? [Re: Nakor420]
    #18948415 - 10/08/13 07:19 AM (10 years, 3 months ago)

Non sterile pour indeed lmao. :P the black stuff is actually some cloth tape i dropped in the pan when making the agar. It burnt up. Oops


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Re: Pan cyan spore syringe? [Re: SupaThaRipper]
    #18948421 - 10/08/13 07:22 AM (10 years, 3 months ago)

Oh I see. :hehehe:

Yeah looks like pan myc...


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OnlineSupaThaRipper
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Re: Pan cyan spore syringe? [Re: Nakor420]
    #18948443 - 10/08/13 07:33 AM (10 years, 3 months ago)

Dont worry buddy, transfering now :smile:


And yeah i was pouring them all in sab but this was the day i broke my collar bone and the pain was unbearable so i said fuck it on the last few plates. Haha


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OfflineNakor420
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Re: Pan cyan spore syringe? [Re: SupaThaRipper]
    #18951645 - 10/08/13 08:37 PM (10 years, 3 months ago)

I see....sorry bout your broken bone...seems you know what you are doing...so yeah...do your transfers and rock on.  :rockon:


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OfflineMush4Brains
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Re: Pan cyan spore syringe? [Re: SupaThaRipper]
    #18951667 - 10/08/13 08:40 PM (10 years, 3 months ago)

Quote:

SupaThaRipper said:
Dont worry buddy, transfering now :smile:


And yeah i was pouring them all in sab but this was the day i broke my collar bone and the pain was unbearable so i said fuck it on the last few plates. Haha





Excellent box dude :thumbup:.

You should post a tutorial on how you made it.

The only downside is the heat off the bulb might cause convection.


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Re: Pan cyan spore syringe? [Re: Mush4Brains]
    #18951671 - 10/08/13 08:41 PM (10 years, 3 months ago)

Interesting thought on convection...being a florescent bulb it is likely cool enough to not be an issue, and if he's just turning it on during his work it won't be heating long enough to move anything.


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Edited by Nakor420 (10/08/13 08:42 PM)


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OfflineMush4Brains
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Re: Pan cyan spore syringe? [Re: Nakor420]
    #18951688 - 10/08/13 08:43 PM (10 years, 3 months ago)

^ All true statements.


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OnlineSupaThaRipper
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Re: Pan cyan spore syringe? [Re: Mush4Brains]
    #18951830 - 10/08/13 09:11 PM (10 years, 3 months ago)

Thanks :wink: unfortunately i dont like it considering you cant use a alcohol lamp in there :/ id rather have a flowhood! :o and leave the gb for unsterile transfers.

And yeah the lamp doesnt generate heat at all while im working.


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OfflineNakor420
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Re: Pan cyan spore syringe? [Re: SupaThaRipper]
    #18951856 - 10/08/13 09:17 PM (10 years, 3 months ago)

Forget alcohol lamps...get yourself one of these bad boys. :laugh:



But be careful...it heats up to 1500 degrees F. This thing will melt your finger off,lol.


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Edited by Nakor420 (10/08/13 09:19 PM)


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OfflineMush4Brains
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Re: Pan cyan spore syringe? [Re: SupaThaRipper]
    #18951875 - 10/08/13 09:22 PM (10 years, 3 months ago)

Quote:

SupaThaRipper said:
Thanks :wink: unfortunately i dont like it considering you cant use a alcohol lamp in there :/ id rather have a flowhood! :o and leave the gb for unsterile transfers.

And yeah the lamp doesnt generate heat at all while im working.




I guess flowhoods seem convenient, but a good SAB is just as effective.


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OfflineNakor420
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Re: Pan cyan spore syringe? [Re: Mush4Brains]
    #18951880 - 10/08/13 09:23 PM (10 years, 3 months ago)

I dropped the loot for a series 1....best thing I ever bought. LOVE it...so much easier than working in that damn sweat box.


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OnlineSupaThaRipper
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Re: Pan cyan spore syringe? [Re: Nakor420]
    #18951909 - 10/08/13 09:29 PM (10 years, 3 months ago)

What is that thing ha it looks like a tiny forced air heater. Link?

Yeah a sab is even more difficult to work in with, as mentioned before, broken collar bone. I seriously dream about working in front of a flowhood... literally dream lol


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OfflineNakor420
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Re: Pan cyan spore syringe? [Re: SupaThaRipper]
    #18951934 - 10/08/13 09:36 PM (10 years, 3 months ago)



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OnlineSupaThaRipper
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Re: Pan cyan spore syringe? [Re: Nakor420]
    #18951952 - 10/08/13 09:41 PM (10 years, 3 months ago)

Damn dude thats badass. Im going to have to get me on of those! Do you have one? How fast does that turn neddles, scalpels, or whatever red hot?


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OfflineNakor420
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Re: Pan cyan spore syringe? [Re: SupaThaRipper]
    #18951960 - 10/08/13 09:43 PM (10 years, 3 months ago)

Instantly....no I don't have one but soon...


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