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OfflineET80s
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Registered: 01/06/13
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contams on agar from syringe but not BRF cakes?
    #18947029 - 10/07/13 09:36 PM (10 years, 3 months ago)

i bought 3 syringes recently just wanting to be able to work with more then one strain.
i thought i would just put a little of each on some agar(i did 3 plates of each) to get them to grain eventually and this happened.



i thought maybe it was the agar batch, so i did another a couple days later and tried again but same thing happened every plate was botched with this stinky yellow crap and some ugly murky slime which ive seen before in some plates of mine but not for over a year.
anyways my question is how does this happen.... i knocked up 20 brf jars between the 3 strains from the same syringes used for the plates and the b+ looks fine.... the others not so much ill toss um, how come i cant get it to go on agar without contams ?
heres a pic of the b+ looking alright




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Invisible8ow8
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Registered: 05/22/13
Posts: 196
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Re: contams on agar from syringe but not BRF cakes? [Re: ET80s]
    #18948552 - 10/08/13 08:26 AM (10 years, 3 months ago)

Happens to me all the time, especially when using rye. A petri dish is an ideal environment - but your brf jars may be a sub-optimal environment for those contams, slowing them down enough that the myc can outgrow them. Once the myc is established, it inhibits most other organisms from developing - sometimes it only needs a little head start to beat out contams.

Back when I was doing multispore syringes, there were a few times I got a cornucopia of wierd stuff on the agar, but just healthy myc on the grain. It seemed to help to use a contam resistant grain (for whatever reason, rye seems to be best), and to keep the hydration level low (NO free water on the surface of the grains).

Stay vigilent, as always. contamination may yet year its ugly head!


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OfflineET80s
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Re: contams on agar from syringe but not BRF cakes? [Re: 8ow8]
    #18951779 - 10/08/13 09:01 PM (10 years, 3 months ago)

thanks for that info , ill dump the rest of what i have into some rye and see how that goes:thumbup:


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