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Offlineslugworth
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Registered: 09/30/13
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Help - Noob - Only few CC's left - salvaging anything
    #18946643 - 10/07/13 08:20 PM (10 years, 4 months ago)

Hey All, so I'm in need of some assistance.

A few weeks ago I started my first batch of BRF using MS...I believe it failed - possibly because I wasn't quick enough with inoculation during open oven procedure, I also may have introduced some mold when wiping with lysol wipe after flaming syringe tip, and/or maybe the TIT (placed inside a luggage chest) got too warm as it was at 84 degrees (in the water, meaning cakes were prolly much warmer and welcoming to contams) for at least a week, and I left the foil on for a week or more.  Those are my hypothesis' at the moment.  I buried all the cakes (except one) yesterday - When I pulled em from the jars they did not smell but they did have a cob webby mold on em, very dry like and firm when attempting to break them up.

Anyways my question to you guys is - One of my jars, seems to have some viable mycellium in it.  You can see it is surrounded by what I believe is mold; but would it be possible to take a biopsy or cutlet of this small chunk and make some sort of LC or other clone project out of it ?  OR is it a complete bust ?  I am desperate and would try anything.

I only had two syringes - the first was lost to this project.  I still have a few cc's in the second one.  I Inocculated 5 jars a few days ago which are colonizing in different areas of the house (I corrected all mistakes listed above and used a glovebox this time - except I did stick myself with the syringe prior to first jar because my gf was coming downstairs and I had to rush to pack everything up - I feel like I am failing miserably with this hobby that I want so bad to have some success with - I am a details type guy but working in a household where the other half/boss is not kosher makes things a tad difficult).  A few CC's were also sacrificed in an attempted honey water LC, fingers crossed this works out so my spore supply will survive.

SO in short if anyone has any suggestions for the small mycellium patch in the contaminated jar or the last few CC's that I do have left (A simple LC tek or otherwise) to extend my supply - ANYTHING would be a great help - I feel like I need a personal mentor



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OfflineMush4Brains
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: slugworth]
    #18946661 - 10/07/13 08:22 PM (10 years, 4 months ago)

Agar.


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Invisiblemaddchef
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: Mush4Brains]
    #18946677 - 10/07/13 08:25 PM (10 years, 4 months ago)

Honestly an lc or agar plates are your real options. Or G2G to expand your remaining spores but boy oh boy do I hope you own a pc. You apparently can steam an lc for x amount of time per RR but I wouldn't know  where to tell you to look.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleNimpo
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: maddchef]
    #18946690 - 10/07/13 08:28 PM (10 years, 4 months ago)

As stated above, LC or agar.

I use my own LC method which hasn't failed or contam'd after 9 different grows. Same as a GLC but in a growbag since i find bags easier.


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OfflineMush4Brains
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: maddchef]
    #18946718 - 10/07/13 08:33 PM (10 years, 4 months ago)

Quote:

maddchef said:
Honestly an lc or agar plates are your real options. Or G2G to expand your remaining spores but boy oh boy do I hope you own a pc. You apparently can steam an lc for x amount of time per RR but I wouldn't know  where to tell you to look.




You can get away with microwaving LC.


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OfflineBlake_Shroom
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: Mush4Brains]
    #18946774 - 10/07/13 08:47 PM (10 years, 4 months ago)

Quote:

Mush4Brains said:
Quote:

maddchef said:
Honestly an lc or agar plates are your real options. Or G2G to expand your remaining spores but boy oh boy do I hope you own a pc. You apparently can steam an lc for x amount of time per RR but I wouldn't know  where to tell you to look.




You can get away with microwaving LC.



That's not something I would advise to someone who can't get the pf tek down. I think an lc is a bad choice here.

I know agar seems really intimidating, it did for me too. I had people tell me to use agar during my first grow/cloning and i did. I fucked up a little bit, but that's how you learn. I see a ton of waste if you try an LC. your innoculant could be contaminated.

Please dont make the mistake that I did in thinking that lc is even an option at this point. Or, at the very least, make an lc and start working with agar. That way you can make sure your culture is clean AND expand your myc:thumbup:


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Offlineslugworth
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: Mush4Brains]
    #18946787 - 10/07/13 08:50 PM (10 years, 4 months ago)

Any good Teks you guys would recommend.  I do not have a pressure cooker.  Can obviously purchase one however the Mrs would find that purchase and use quite precarious - we also have a glass stove top which from my understanding can pose an issue for the PC's (maybe only large ones ?).  For more discreet operations which I can easily move to the basement I have researched some electric PC's, some members have attested to their usefulness but I wasn't able to find any at target, wegmans, kmart or Big lots.  Need to get out to Bed bath and beyond. 

So for now I am stuck with steaming.  I have read some on g2g however the tek I read did not specify what exactly you purchase for a grain  ?  Why is g2g more reliable vs BRF ?  Best Tek recommendations ?

As per Agar, is this exclusively available online or are there common stores that might carry this ?  How about some jello ?

Any ideas if that little rhizomorphic spot in the one jar would be worth scraping and tossing in a jar to see if it colonizes into anything or complete loss ?


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Invisiblemaddchef
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: Mush4Brains]
    #18946790 - 10/07/13 08:50 PM (10 years, 4 months ago)

Doesn't surprise me since its a liquid. OP just don't be one of the tools that puts metal jar lids in your microwave radiation producing device.

Blake, I only say an lc because the op probably has all the stuff he needs around the house. As it sounds like he is too cheap or broke to order more spores, therefore too broke for agar supplies.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Invisiblemaddchef
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: maddchef]
    #18946798 - 10/07/13 08:52 PM (10 years, 4 months ago)

Leave the jar be, without agar its a lost cause. If you don't own a pc, disregard grain and G2G.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlineslugworth
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Registered: 09/30/13
Posts: 37
Last seen: 10 years, 2 months
Re: Help - Noob - Only few CC's left - salvaging anything [Re: slugworth]
    #18946806 - 10/07/13 08:53 PM (10 years, 4 months ago)

I have a honey LC brewing currently, I forget exactly which Tek I followed but it was one of the more commented ones, presumably a relatively successful procedure, pending my own human error that is.  I have a fresh bag of cracked corn if anyone knows how newb friendly this substrate is.


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Invisiblemaddchef
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: slugworth]
    #18946812 - 10/07/13 08:55 PM (10 years, 4 months ago)

Leave the corn alone as well without a pc.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlineslugworth
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Registered: 09/30/13
Posts: 37
Last seen: 10 years, 2 months
Re: Help - Noob - Only few CC's left - salvaging anything [Re: slugworth]
    #18946862 - 10/07/13 09:05 PM (10 years, 4 months ago)

Finances not the issue - have to operate in secrecy.  I work in healthcare and didn't wanna risk any tracing of suspicious mycological specimens being sent to the house, enemy number one being the Mrs asking what the hell came in the package today - this is also why I'm struggling with acquisition of a PC.  I have been looking into purchasing a hot plate which I could use in the basement (where she does not frequent) but not sure how that would work with PC i.e would it get warm enough.

My original spores were ordered by a friend who then sent them to a friends residence where I then picked them up.  Issue is busy life schedules and again the Mrs, make acquisition of more spores an annoying process, hence why I am so desperate get something rolling that I can LC from.


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OfflineBlake_Shroom
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: slugworth]
    #18946899 - 10/07/13 09:13 PM (10 years, 4 months ago)

Well, you have a fundamental issue my friend.

Is growing fungus worth risking your relationship?

That's the main issue now man. Take a good look at the pro's and con's and make a choice. You honestly wont be able to have a stealth grow going with your lady present. If she is someone you care about, then ditch the operation. Sucks, but angry girls can not only ruin your grow, but I would say that she will go further than just throwing away your stuff once she finds out


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InvisibleNimpo
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: slugworth]
    #18946902 - 10/07/13 09:14 PM (10 years, 4 months ago)

OP, Im doubting your "wife" excuse. If you live with parents, its alright to say.

No grown ass married man would be hesitant to order something to his own house. If she asks, its a surprise. Then, go buy her a surprise. Or make one. Marriage is about two people, right? Fill her in with what you are doing. How do you expect to fruit these bad boys if your wife cant know? How will she react when she stumbles across it?

Might as well just be upfront with her now and spare yourself a breakup, an angry discussion, or another contaminated grow.

Quote:

I work in healthcare and didn't wanna risk any tracing of suspicious mycological specimens being sent to the house




Its legal. :ancientaliens:

Buy a Visa giftcard with 25 bucks on it and use that to buy a syringe or two.


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OfflineSynKyd
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: Nimpo]
    #18946989 - 10/07/13 09:29 PM (10 years, 4 months ago)

Quote:

Nimpo said:

Might as well just be upfront with her now and spare yourself a breakup, an angry discussion, or another contaminated grow.





Truer words have never been typed! Get it out there and talk to her if you are even considering a grow.


--------------------

New inclusive poop emojis from Apple!

:shpongle::shpongle::shpongle::shpongle::shpongle:



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OfflineMush4Brains
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: Blake_Shroom]
    #18947263 - 10/07/13 10:28 PM (10 years, 4 months ago)

Quote:

Blake_Shroom said:
That's not something I would advise to someone who can't get the pf tek down. I think an lc is a bad choice here.

I know agar seems really intimidating, it did for me too. I had people tell me to use agar during my first grow/cloning and i did. I fucked up a little bit, but that's how you learn. I see a ton of waste if you try an LC. your innoculant could be contaminated.

Please dont make the mistake that I did in thinking that lc is even an option at this point. Or, at the very least, make an lc and start working with agar. That way you can make sure your culture is clean AND expand your myc:thumbup:




Yeah, I wasn't necessarily advocating an LC, just that you can get away with microwaving an LC.  You can also get away with antibiotic agar in the microwave as well.  Remember, even when we're sterilizing in a PC at 15PSI for 90minutes we're not achieving 100% sterilization if I recall correctly.

http://www.kitchenculturekit.com/Mushroom/agarplates.htm
http://www.nytimes.com/2007/03/27/health/27real.html?_r=0
http://www.sciencedaily.com/releases/2007/04/070423120145.htm
http://www.thefreelibrary.com/Microbial+inactivation+by+microwave+radiation+in+the+home+environment.-a0156366249

If you can kill bacteria and molds on a sponge, I don't see why you can't kill bacteria and molds in a liquid solution, a couple rounds of five minutes a piece should be satisfactory.  I've never personally tried microwaving agar, but before I had a PC doing PF Tek I did successfully do honey LCs.  Once I get access to the marketplace and trade for some petri dishes, I'll run some tests.

But this is neither here nor there.  OP get a pressure cooker.  Do things the right way or be plagued by contamination issues which will result in you quitting the hobby. 


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Offlineslugworth
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: Mush4Brains]
    #18948913 - 10/08/13 10:25 AM (10 years, 4 months ago)

Thanks for the input guys. The fundamental issue is in the works - its been in the works for years.  She is quite conservative with things of this nature however she is coming around in other avenues.  She has been asking what ive been researching lately - this isn't anything new as I'm always researching something however I'm sure you guys can attest to the amount of time and effort it takes to get started in this hobby.  I really appreciate the words of wisdom to bring it to light - this will certainly make life easier - its just a matter of finding a time when her mood is appropriate - hopefully in the next week or two well breach the topic, successfully. In the mean time stealth mode it is.

As for microwaving lc, any recs for a substrate / tek ?


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Offlineslugworth
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: slugworth]
    #18978857 - 10/14/13 09:35 PM (10 years, 4 months ago)

So after some chatting with the Mrs ive officially been cleared :ooo:!!! Thanks for the encouragement guys, likely would have taken much longer without it. Totally pumped to get things rolling for real now.  Ive had five brf jars and the honey lc colonizing for 11 days  now and there is not a spec of anything in a single jar.given my first set using a different syringe had a good deal of growth by the 4th day I'm wondering if this syringe must have been a complete Dud. Every single jar looks exactly as it did when they came out of the cook. Temps have been a bit lower(62-70) this time since I didn't use the Tit but I figured something would be growing by now ?  Figuring i have nothing to lose I put 2/5 in the Tit set at 75 degrees f.  I also shook up the syringe, flamed then put 3 more cc's in the honey hoping something will come of it

Anyone had this happen before ?


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OfflineSchmendrick
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Re: Help - Noob - Only few CC's left - salvaging anything [Re: slugworth]
    #18978935 - 10/14/13 09:51 PM (10 years, 4 months ago)

Quote:

slugworth said:
So after some chatting with the Mrs ive officially been cleared :ooo:!!! Thanks for the encouragement guys, likely would have taken much longer without it. Totally pumped to get things rolling for real now.  Ive had five brf jars and the honey lc colonizing for 11 days  now and there is not a spec of anything in a single jar.given my first set using a different syringe had a good deal of growth by the 4th day I'm wondering if this syringe must have been a complete Dud. Every single jar looks exactly as it did when they came out of the cook. Temps have been a bit lower(62-70) this time since I didn't use the Tit but I figured something would be growing by now ?  Figuring i have nothing to lose I put 2/5 in the Tit set at 75 degrees f.  I also shook up the syringe, flamed then put 3 more cc's in the honey hoping something will come of it

Anyone had this happen before ?




You might think you're in the clear, but take it from me... don't set up your grow in the kitchen.  Even if your wife is totally fine with it like mine was/is, if you start taking up counter space with jars, tubs, colonizing trays, fruiting chambers, stacks of petri dishes, hypodermic needles, scalpels and bottles af Agar... you are gonna have a problem :wink:

Also, get another fridge for your hobby... the wife also doesn't appreciate all of the door space being taken up by liquid cultures.

Even if you do most of the cooking (like I do), as soon as you start doing the above stuff, you will be surprised how fast it becomes "her kitchen" again :grin:


Edited by Schmendrick (10/14/13 09:55 PM)


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