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InvisibleImperfect Iam
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Cleaning your sab
    #18943008 - 10/07/13 12:29 AM (10 years, 3 months ago)

I don't know if this is the best place to post this but here goes I have made a decent sab and I am still getting cont. Im pretty sure this is caused by incorrect usage I clean it with soap and water then let it dry and spray with Lysol and let sit for 20-30 min then wipe out the Lysol with a napkin. I then put a paper towel with 70% alcohol in it and wash my supplies with alcohol and load them through holes for your arms. I have been growing wild gourmets (or trying) starting on agar with pieces of mushrooms now I know wild mushrooms are cont. but I guess what I'm getting at is say I just put a piece of tissue from one wild mushroom in a petri said mushroom is in box  and I move onto the next pulling the petri out do I need to respray with Lysol and wipe out again for another petri from same mushroom? Also when I'm done with one mushroom and move on to a diff. One does it need to be resprayed and wiped again? One more I have grown out the usually cont. mycelium and am going to do transfers if I have 2 cont. plates after I cut out a wedge from cont. plate and  put in clean plate do I need to respray  and wipe again and should the alcohol soaked paper towel be replaced? Confusing I know but could not figure a better way to ask.


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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InvisibleLiquidGlass
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Re: Cleaning your sab [Re: Imperfect Iam]
    #18943094 - 10/07/13 01:11 AM (10 years, 3 months ago)

Are you cutting tissue from INSIDE the mushroom Cause if not you should be.


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InvisibleImperfect Iam
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Re: Cleaning your sab [Re: LiquidGlass]
    #18943134 - 10/07/13 01:33 AM (10 years, 3 months ago)

Yes the best i can with a scalpel I think maybe tweezers would be wise to use also thought because I have prob pulling the tissue out I also have prob. W/ getting sample from petri out and off scalpel into fresh petri maybe two scalpels would be the answer to that prob or tweezers


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


Edited by Imperfect Iam (10/07/13 01:40 AM)


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InvisibleLiquidGlass
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Re: Cleaning your sab [Re: Imperfect Iam]
    #18943196 - 10/07/13 02:16 AM (10 years, 3 months ago)

I have had good luck using a half pint widemouth mason jar with about a 1/4 inch hole in the lid stuffed tightly with polyfil, and once everything is clean and in the SAB I will take the tissue sample, use teezers dip it in a petri dish filled with hydrogen peroxide, and submerge it for 3-5 seconds before placing on the agar


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Some art I've made Glass Art Gallery

I was raised a christian and was a stone-faced acid head
- Ken Kesey


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Re: Cleaning your sab [Re: LiquidGlass]
    #18943207 - 10/07/13 02:20 AM (10 years, 3 months ago)

As for the pre-cleaning. I saturate some paper towels and wipe down the whole thing inside and out, then I take a 55 gallon plastic drum liner bag and put the tub inside laying on its side, then I wipe everything down as I place it into the box. Then I tie the bag closed and very quickly cut a window on the top( which is actually the side of the tub) and carefully and quickly tape the edges. In the bottom of the tub(which is now facing you) I have made 2 arm holes and I make 2 tiny incisions and force my arms through and the plastic bag is sealed tight around my arms. Then I do the work


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Some art I've made Glass Art Gallery

I was raised a christian and was a stone-faced acid head
- Ken Kesey


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Re: Cleaning your sab [Re: LiquidGlass]
    #18943211 - 10/07/13 02:21 AM (10 years, 3 months ago)

I also use lots of lysol neutra-air or Oust in the room and also inside the tub right before I tie up the bag around it


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Some art I've made Glass Art Gallery

I was raised a christian and was a stone-faced acid head
- Ken Kesey


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Re: Cleaning your sab [Re: LiquidGlass]
    #18943232 - 10/07/13 02:37 AM (10 years, 3 months ago)

Id leave the inner surface of the sab wet too so all the icky shit gets stuck to it and not your petri dish.


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I fucking hate grammer nazis! Yes, I can't spell. Yes, I don't have perfect grammer. I post from my phone and dont give a shit about people whose lifes are so boring they get off on putting people down for not having perfect fucking grammer, even though they know excactly what there saying.. Fuck You. It's just a ride mang...


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InvisibleImperfect Iam
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Re: Cleaning your sab [Re: huffinglue]
    #18943291 - 10/07/13 03:19 AM (10 years, 3 months ago)

So it sounds like you are making a Sab in a sab but with a bag right? And the last one you answered I was thinking about doing that but I figured that was were all the cont. we're laying so cont. would get on the petri and climb in but it doesn't sound like that is the case. I will have to try that, your talking about the bottom also where your petri is sitting correct?


Edited by Imperfect Iam (10/07/13 03:22 AM)


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Re: Cleaning your sab [Re: LiquidGlass]
    #18943312 - 10/07/13 03:36 AM (10 years, 3 months ago)

Quote:

LiquidGlass said:
I have had good luck using a half pint widemouth mason jar with about a 1/4 inch hole in the lid stuffed tightly with polyfil, and once everything is clean and in the SAB I will take the tissue sample, use teezers dip it in a petri dish filled with hydrogen peroxide, and submerge it for 3-5 seconds before placing on the agar



I figured this would kill the tissue sample but apparently not, it sounds as if I need to go find me some mushrooms today and try this I know the oysters have got to be out as we just had a cold snap and on my last post I didn't see someone else had wrote that about not wiping it out after u spray it but it sounds as if you don't either also I was raised catholic and was a stoned face acid head myself lol!


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Cleaning your sab [Re: Imperfect Iam]
    #18943677 - 10/07/13 07:23 AM (10 years, 3 months ago)

Haha I wasnt raised catholic, I was baptist, a little more relxed but still ended up an acid head haha

And no I dont really dry it from the alcohol so as huffinglue mentioned, the bad stuff is more likely to stick to it than float around, and peroxide will not kill live mycelium/tissue.


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Some art I've made Glass Art Gallery

I was raised a christian and was a stone-faced acid head
- Ken Kesey


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InvisibleImperfect Iam
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Re: Cleaning your sab [Re: LiquidGlass]
    #18943733 - 10/07/13 08:07 AM (10 years, 3 months ago)

Kick ass I'm looking for some now I may have found one I'm making print now going to post now in I'd forum idk though but it resembles p. cinctulus will see in a sec. Maybe


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Cleaning your sab [Re: Imperfect Iam]
    #18946686 - 10/07/13 08:27 PM (10 years, 3 months ago)

Not even the bottom were you set your Petris and equipment? Ps I made the print up and it is not p.cinctulus the spore print was brown;(


Edited by Imperfect Iam (10/07/13 08:30 PM)


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Re: Cleaning your sab [Re: Imperfect Iam]
    #18947490 - 10/07/13 11:14 PM (10 years, 3 months ago)

You should be flame sterilizing your scalpel.

You only need to spray box before you start using it. It helps to leave the bottom and sides of the box wet while you work. (with soap and water or 10% bleach)

When cloning a mushroom you should assume it's contaminated and transfer the mushroom mycelium to a new dish before any contaminants have time to show up.



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Re: Cleaning your sab [Re: LiquidGlass]
    #18948064 - 10/08/13 03:31 AM (10 years, 3 months ago)

I was referring to your sig but now I see it was a quote from a guy called Ken Kesey still I like it. Yeah I hated going to church every Sunday but I always loved communion time it meant it was almost over but the best part was checking out the hot ass chicks in there Sunday best about 12 years old sitting in church thinking of how I'd work them over good! LMAO


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Cleaning your sab [Re: Kizzle]
    #18948079 - 10/08/13 03:40 AM (10 years, 3 months ago)

But I'm still wondering do you clean off were your setting all your stuff as in the bottom of the Sab and I wonder is it so necessary work in a box in a box can't hurt I'm sure but I already think its uncomfortable and hard to see in, the only color I could find Is clear but it is still opaque!


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Cleaning your sab [Re: Imperfect Iam]
    #18948082 - 10/08/13 03:42 AM (10 years, 3 months ago)

I knew that you were referring to my sig, do you not know who ken kesey was? He was a the author who wrote "One Flew Over the Cuckoos Nest" (awesome book) and also the main subject of "The Electric Kool-Aid Acid Test" another awesome book


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Some art I've made Glass Art Gallery

I was raised a christian and was a stone-faced acid head
- Ken Kesey


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Re: Cleaning your sab [Re: Imperfect Iam]
    #18948085 - 10/08/13 03:43 AM (10 years, 3 months ago)

Quote:

cc1 said:
But I'm still wondering do you clean off were your setting all your stuff as in the bottom of the Sab and I wonder is it so necessary work in a box in a box can't hurt I'm sure but I already think its uncomfortable and hard to see in, the only color I could find Is clear but it is still opaque!




I hold the tools the whole time and I have the mushroom in a ziplock before placing it in the SAB, I do not dry the bottom of the tub


--------------------
Some art I've made Glass Art Gallery

I was raised a christian and was a stone-faced acid head
- Ken Kesey


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Re: Cleaning your sab [Re: LiquidGlass]
    #18948118 - 10/08/13 04:12 AM (10 years, 3 months ago)

Oh I think I see so you just open up the ziplock to do your work correct?


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Cleaning your sab [Re: Imperfect Iam]
    #18948186 - 10/08/13 05:00 AM (10 years, 3 months ago)

Just spray the inside down, wait 10 minutes, wipe top dry so it doesn't drip. The arm holes should be wide enough that you can easily pull your arms in and out to flame sterilize tools when necessary.

If it's too hard to see you can cut out a little viewing window and tape some saran wrap or something similar over it.


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Re: Cleaning your sab [Re: LiquidGlass]
    #18952002 - 10/08/13 09:54 PM (10 years, 3 months ago)

No I didn't know that one flew over the cuckoos nest was great as far as the electric kool-aid I'm going to have to read that one all this by a stone face acid head no wonder acid is illegal ruins your life obviusly


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Cleaning your sab [Re: Kizzle]
    #18952018 - 10/08/13 09:56 PM (10 years, 3 months ago)

And yes I am flame sterilizing my scalpel


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Cleaning your sab [Re: Kizzle]
    #18956810 - 10/09/13 09:56 PM (10 years, 3 months ago)

Yeah being opaque it is hard to see I guess Saran Wrap would work but I would probably want to use some thin plexiglass, more for looks than anything good idea thought. I bought some wet wipes says kills 99.9% germs I wonder if that would be good shit for cleaning inside of box and your equipment? I'm getting ready to load in some jars and I think I will use that to wipe them down. Oh by the way I revived your thread on cont. because those pics were way cool but I wanted to add I wish I would have taken pics of some of the shit I had in Petris for months, I didn't realize how dangerous some of this shit was didn't know if you saw that or not?


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Cleaning your sab [Re: Imperfect Iam]
    #18957648 - 10/10/13 01:25 AM (10 years, 3 months ago)

Alcohol's not a very good disinfect. The "kills 99.9% of germs" claims on certain products are misleading. Usually they're only referring to certain bacteria and viruses.

Fortunately it's not even necessary to kill the contaminants. On a material like plastic the vast majority are removed just by normal cleaning methods.


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Re: Cleaning your sab [Re: Kizzle]
    #18957713 - 10/10/13 02:19 AM (10 years, 3 months ago)

Quote:

Usually they're only referring to certain bacteria




That is exactly why I use it, I have had more contams from bacteria than from mold and using alcohol seems to help, or at least in my own head it does . . .


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- Ken Kesey


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Re: Cleaning your sab [Re: Kizzle]
    #18959471 - 10/10/13 01:42 PM (10 years, 3 months ago)

I just figured those wet wipes would be nice and easy but I guess no easier than using a soapy rag because I came to find it kind of leaves a sudsy residue on your equipment. I think now I am going overboard trying to keep cont. out, but I did my third agar pour w/ soufflé cups(the reason behind the soufflé cups is in another post but first two agar pours I got a lot of mold the third one 0% cont. rate! I'm making progress I was starting to think this was above my capabilities but 50 cups 0 cont. I am pretty proud!


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Cleaning your sab [Re: Kizzle]
    #18967124 - 10/12/13 04:26 AM (10 years, 3 months ago)

How many times do u usually transfer a wild mushrooms running mycelium? Just until you see no cont. are is there a minimum you always do, before transferring to grains? Also do you always transfer before it hits the sidewalls of plates as in my experience they almost always cont. when it hits, I know what Paul Stamets says but would like your opinion!


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Cleaning your sab [Re: Imperfect Iam]
    #18968871 - 10/12/13 03:45 PM (10 years, 3 months ago)

IF it stops sectoring you have an isolate, like if it grows in a perfice circle, but if the ciclre isnt round and grows toward the edge faster in some spots you can keep transferring


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I was raised a christian and was a stone-faced acid head
- Ken Kesey


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Re: Cleaning your sab [Re: LiquidGlass]
    #18969679 - 10/12/13 07:55 PM (10 years, 3 months ago)

Cool thanks man! I transferred to grains on 2nd transfer of turkey tail and on 2nd day got a green cont., I was wondering, should I pitch the grains now so I can save jar, are can I wait and see what the t.t. mycelium does to it. I know that's probably a stupid question, just figured I would ask, it's not like t.t. Are hard to come by here. I also had made another transfer to agar that took off like a mofo, so I do have a backup.


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Cleaning your sab [Re: Imperfect Iam]
    #18969885 - 10/12/13 08:48 PM (10 years, 3 months ago)

You want to do one transfer as soon as the first mycelium starts spreading outward just to isolate it from contaminants which show up shortly after the mushroom mycelium. I wouldn't even wait to see if any contaminants start growing or it's just that much harder to isolate from them.


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Re: Cleaning your sab [Re: Kizzle]
    #18969992 - 10/12/13 09:21 PM (10 years, 3 months ago)

Thanks Kizzle, but about the grains that I transferred to that have a green cont. started in them, go ahead and pitch correct? I want to save the jars but kind of wanted to see what the turkey tail mycelium would do to it, as I have only 10 jars go ahead and pitch, and jars can be saved right? Ps I am going to have some pics on cont. in Petris soon (And soufflé cups) but nothing compared to yours I double zip locked bagged them so everything should be ok?


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Cleaning your sab [Re: Imperfect Iam]
    #18970350 - 10/12/13 10:57 PM (10 years, 3 months ago)

You can reuse jars. Just don't open mold contaminated jars indoors unless you sterilize them first.


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Re: Cleaning your sab [Re: Kizzle]
    #18970915 - 10/13/13 02:15 AM (10 years, 3 months ago)

Meaning dump the grains in a ziplock Baggie seal throw in trash, then wipe out with alcohol, would that be good? Then once inside, put in PC for a while then pull them out and clean w:/ soapy water, then rinse out soap? I'm kind of confused? I know I can look up old threads but you seem to know wtf your doing for sure! I have read and read, but speaking with someone who has def. ran across a multitude of cont. makes me feel 100% better, hell I just read a thread talking about a pink cont. that gives you spinal meningitis, fuck me! It almost scares me enough to quit and I am loving this hobby! Again thanks Kizzle


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Cleaning your sab [Re: Imperfect Iam]
    #18970983 - 10/13/13 03:02 AM (10 years, 3 months ago)

pc the contamed grain jars before opening them so you dont release extra spore in your house


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I was raised a christian and was a stone-faced acid head
- Ken Kesey


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Re: Cleaning your sab [Re: LiquidGlass]
    #18971090 - 10/13/13 04:39 AM (10 years, 3 months ago)

Gotcha I am gonna figure this out eventually, I do believe moving onto diff. Species are going to be a lot easier after all these cont, and wonderful people such as yourself giving me a big hand. I have always pretty much been interested in growing my own mushrooms since about the age of 15 ( but back then only concerned of one kind LOL) as I read high times a lot And would see the grow kits in there and read up on it a little back then and seen you pretty much had to have a PC, it was game over, and my parents would not have approved, and PCs were out of my price range back then. That was app. The year 1992. A LOT has changed since then and finding Paul stamets book GG&MM made me think I can do this, so I have most all materials, books, and equipment for doing this now! It has been a lot of trial and error but I am getting closer everyday, I have done one grow of pearl oysters from a kit, if you have any interest as to what I did with the mushrooms besides eat them there is a post in gourmet and medicinal mushrooms. It is labeled prickly pear used in substrate, I think you might enjoy it. Anyway I'm working on reishi, enoki, and turkey tail ATM and if I can get these wild ones figured out, I,m pretty sure if someone would make a trade with me for some cubes or something of that nature I' m pretty sure I could pull it off no prob. I could always buy a syringe ore filled with spores, but I want to get fully in on this site and start some trading I can't wait! Ps sorry for always the long and not very well written posts but I have a hard time getting my point across in few words thanks so much for all the help I' sure I' m not done asking yet but don't feel as if you have to reply, but i would def. be bummed to quit hearing from you if any of my labors come to fruit or you want any wild gourmet prints or Petris grown out w/ mycelium don't hesitate to ask I will do what I can to come up with what you would like u deserve it no doubt peace


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Cleaning your sab [Re: Imperfect Iam]
    #18971101 - 10/13/13 04:49 AM (10 years, 3 months ago)

How that pic. Got in there I don't know, but have been trying to make it my avatar for 3 days now! Internet has been shut off I am on cell and can't get it sized right, and now they want me to push radio button, as If I know how to do that even if Internet was working, hell I'm sure you can do it from cell.I am just computer illiterate, but working on that also


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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InvisibleLiquidGlass
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Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
Re: Cleaning your sab [Re: Imperfect Iam]
    #18971145 - 10/13/13 05:26 AM (10 years, 3 months ago)

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