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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Cleaning your sab
#18943008 - 10/07/13 12:29 AM (10 years, 4 months ago) |
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I don't know if this is the best place to post this but here goes I have made a decent sab and I am still getting cont. Im pretty sure this is caused by incorrect usage I clean it with soap and water then let it dry and spray with Lysol and let sit for 20-30 min then wipe out the Lysol with a napkin. I then put a paper towel with 70% alcohol in it and wash my supplies with alcohol and load them through holes for your arms. I have been growing wild gourmets (or trying) starting on agar with pieces of mushrooms now I know wild mushrooms are cont. but I guess what I'm getting at is say I just put a piece of tissue from one wild mushroom in a petri said mushroom is in box and I move onto the next pulling the petri out do I need to respray with Lysol and wipe out again for another petri from same mushroom? Also when I'm done with one mushroom and move on to a diff. One does it need to be resprayed and wiped again? One more I have grown out the usually cont. mycelium and am going to do transfers if I have 2 cont. plates after I cut out a wedge from cont. plate and put in clean plate do I need to respray and wipe again and should the alcohol soaked paper towel be replaced? Confusing I know but could not figure a better way to ask.
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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LiquidGlass
Glass Blower


Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
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Are you cutting tissue from INSIDE the mushroom Cause if not you should be.
-------------------- Some art I've made Glass Art Gallery
  I was raised a christian and was a stone-faced acid head - Ken Kesey
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Yes the best i can with a scalpel I think maybe tweezers would be wise to use also thought because I have prob pulling the tissue out I also have prob. W/ getting sample from petri out and off scalpel into fresh petri maybe two scalpels would be the answer to that prob or tweezers
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
Edited by Imperfect Iam (10/07/13 01:40 AM)
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LiquidGlass
Glass Blower


Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
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I have had good luck using a half pint widemouth mason jar with about a 1/4 inch hole in the lid stuffed tightly with polyfil, and once everything is clean and in the SAB I will take the tissue sample, use teezers dip it in a petri dish filled with hydrogen peroxide, and submerge it for 3-5 seconds before placing on the agar
-------------------- Some art I've made Glass Art Gallery
  I was raised a christian and was a stone-faced acid head - Ken Kesey
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LiquidGlass
Glass Blower


Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
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As for the pre-cleaning. I saturate some paper towels and wipe down the whole thing inside and out, then I take a 55 gallon plastic drum liner bag and put the tub inside laying on its side, then I wipe everything down as I place it into the box. Then I tie the bag closed and very quickly cut a window on the top( which is actually the side of the tub) and carefully and quickly tape the edges. In the bottom of the tub(which is now facing you) I have made 2 arm holes and I make 2 tiny incisions and force my arms through and the plastic bag is sealed tight around my arms. Then I do the work
-------------------- Some art I've made Glass Art Gallery
  I was raised a christian and was a stone-faced acid head - Ken Kesey
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LiquidGlass
Glass Blower


Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
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I also use lots of lysol neutra-air or Oust in the room and also inside the tub right before I tie up the bag around it
-------------------- Some art I've made Glass Art Gallery
  I was raised a christian and was a stone-faced acid head - Ken Kesey
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huffinglue
tryin to stay sober


Registered: 09/26/08
Posts: 450
Loc: Texas
Last seen: 9 years, 6 months
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Id leave the inner surface of the sab wet too so all the icky shit gets stuck to it and not your petri dish.
-------------------- I fucking hate grammer nazis! Yes, I can't spell. Yes, I don't have perfect grammer. I post from my phone and dont give a shit about people whose lifes are so boring they get off on putting people down for not having perfect fucking grammer, even though they know excactly what there saying.. Fuck You. It's just a ride mang...
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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So it sounds like you are making a Sab in a sab but with a bag right? And the last one you answered I was thinking about doing that but I figured that was were all the cont. we're laying so cont. would get on the petri and climb in but it doesn't sound like that is the case. I will have to try that, your talking about the bottom also where your petri is sitting correct?
Edited by Imperfect Iam (10/07/13 03:22 AM)
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Quote:
LiquidGlass said: I have had good luck using a half pint widemouth mason jar with about a 1/4 inch hole in the lid stuffed tightly with polyfil, and once everything is clean and in the SAB I will take the tissue sample, use teezers dip it in a petri dish filled with hydrogen peroxide, and submerge it for 3-5 seconds before placing on the agar
I figured this would kill the tissue sample but apparently not, it sounds as if I need to go find me some mushrooms today and try this I know the oysters have got to be out as we just had a cold snap and on my last post I didn't see someone else had wrote that about not wiping it out after u spray it but it sounds as if you don't either also I was raised catholic and was a stoned face acid head myself lol!
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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LiquidGlass
Glass Blower


Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
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Haha I wasnt raised catholic, I was baptist, a little more relxed but still ended up an acid head haha
And no I dont really dry it from the alcohol so as huffinglue mentioned, the bad stuff is more likely to stick to it than float around, and peroxide will not kill live mycelium/tissue.
-------------------- Some art I've made Glass Art Gallery
  I was raised a christian and was a stone-faced acid head - Ken Kesey
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Kick ass I'm looking for some now I may have found one I'm making print now going to post now in I'd forum idk though but it resembles p. cinctulus will see in a sec. Maybe
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Not even the bottom were you set your Petris and equipment? Ps I made the print up and it is not p.cinctulus the spore print was brown;(
Edited by Imperfect Iam (10/07/13 08:30 PM)
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,865
Last seen: 1 day, 20 hours
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You should be flame sterilizing your scalpel.
You only need to spray box before you start using it. It helps to leave the bottom and sides of the box wet while you work. (with soap and water or 10% bleach)
When cloning a mushroom you should assume it's contaminated and transfer the mushroom mycelium to a new dish before any contaminants have time to show up.
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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I was referring to your sig but now I see it was a quote from a guy called Ken Kesey still I like it. Yeah I hated going to church every Sunday but I always loved communion time it meant it was almost over but the best part was checking out the hot ass chicks in there Sunday best about 12 years old sitting in church thinking of how I'd work them over good! LMAO
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Re: Cleaning your sab [Re: Kizzle]
#18948079 - 10/08/13 03:40 AM (10 years, 4 months ago) |
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But I'm still wondering do you clean off were your setting all your stuff as in the bottom of the Sab and I wonder is it so necessary work in a box in a box can't hurt I'm sure but I already think its uncomfortable and hard to see in, the only color I could find Is clear but it is still opaque!
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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LiquidGlass
Glass Blower


Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
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I knew that you were referring to my sig, do you not know who ken kesey was? He was a the author who wrote "One Flew Over the Cuckoos Nest" (awesome book) and also the main subject of "The Electric Kool-Aid Acid Test" another awesome book
-------------------- Some art I've made Glass Art Gallery
  I was raised a christian and was a stone-faced acid head - Ken Kesey
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LiquidGlass
Glass Blower


Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
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Quote:
cc1 said: But I'm still wondering do you clean off were your setting all your stuff as in the bottom of the Sab and I wonder is it so necessary work in a box in a box can't hurt I'm sure but I already think its uncomfortable and hard to see in, the only color I could find Is clear but it is still opaque!
I hold the tools the whole time and I have the mushroom in a ziplock before placing it in the SAB, I do not dry the bottom of the tub
-------------------- Some art I've made Glass Art Gallery
  I was raised a christian and was a stone-faced acid head - Ken Kesey
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Oh I think I see so you just open up the ziplock to do your work correct?
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,865
Last seen: 1 day, 20 hours
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Just spray the inside down, wait 10 minutes, wipe top dry so it doesn't drip. The arm holes should be wide enough that you can easily pull your arms in and out to flame sterilize tools when necessary.
If it's too hard to see you can cut out a little viewing window and tape some saran wrap or something similar over it.
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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No I didn't know that one flew over the cuckoos nest was great as far as the electric kool-aid I'm going to have to read that one all this by a stone face acid head no wonder acid is illegal ruins your life obviusly
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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