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Offlinemushbecrazy
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liquid cultures
    #18939343 - 10/06/13 09:14 AM (10 years, 3 months ago)

Can i just put the whole rye berry covered in myceliym. Or is it better scrap some off. Im using 1tea spoon honey per cup water. Brought to boil poured in jars PC'ed 20mins. Planing on just dumping a couple berrie a jar. Last thing can i put peroxide in there? If so how much a cup?


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OfflineRauhfasertapete
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Re: liquid cultures [Re: mushbecrazy]
    #18939365 - 10/06/13 09:21 AM (10 years, 3 months ago)

the method is not doable, its necessarily unsterile; an LC has to be sterile as possible. Why would one want to try to turn a cake into a LC? Either spawn it, take spores and put these on agar, or put a few grains from your cake on agar.

don´t use peroxide on your mycelium, it just damages any living tissue it gets in contact with.


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InvisiblePastywhyteMDiscord
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Re: liquid cultures [Re: mushbecrazy]
    #18939370 - 10/06/13 09:23 AM (10 years, 3 months ago)

Quote:

mushbecrazy said:
Can i just put the whole rye berry covered in myceliym.




You could but it would be better to put that on agar if you want to be sure of clean inoculate.

Quote:

mushbecrazy said:
Or is it better scrap some off.




:nojustno:

Quote:

mushbecrazy said:
Last thing can i put peroxide in there? If so how much a cup?



I wouldn't. The only use peroxide has in mycology is battling cobweb.

Really if your just growing cubes I would just go straight to agar. This will let you be sure your inoculate is clean. Then knock up a jar with a wedge, then g2g. Will be pretty much as fast as LC in the end but a lot less risk along the way.

IMO LC is risky. I am planning to experiment with it for species that cannot be shaken but, if I was just growing cubes the benefits are minimal compared to the risks :shrug:


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Offlinemushbecrazy
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Re: liquid cultures [Re: Pastywhyte]
    #18939392 - 10/06/13 09:32 AM (10 years, 3 months ago)

Pan trop. Is what im trying now. Ive got a few ms agar plates and a couple with A rye berry. Was just hopeing to get a jump start. Also made a horsepoo/straw cake 2ins thick seems to be colonize pretty quick. Heard they were offal slow.


--------------------
I'm just some lunatic macaroni mushroom, is that it?
-Joe Pesci


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OfflineRauhfasertapete
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Re: liquid cultures [Re: Pastywhyte]
    #18939397 - 10/06/13 09:34 AM (10 years, 3 months ago)

Quote:

Pastywhyte said:
I am planning to experiment with it for species that cannot be shaken




what kind of creature is that and why can´t you shake it?


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Offlinemushbecrazy
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Re: liquid cultures [Re: Rauhfasertapete]
    #18939401 - 10/06/13 09:35 AM (10 years, 3 months ago)

I was wondering that to. Maybe a truffle


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Re: liquid cultures [Re: mushbecrazy]
    #18939415 - 10/06/13 09:39 AM (10 years, 3 months ago)

Well my pan experience is pretty limited but I have found that pans can be fast or slow. My best attempt at pans never needed to be shook they colonized so hard and fast. I do believe that temps were part of that equation, everything that stalled on me did so in lower temps (70-74F) and the stuff that took off, did so in summer where my indoor temps were around 75-78F. I have heard of people liking LC for pans but if it was me, I would still use a wedge to inoculate my LC. I plan to do some LC for p mexicana soon as they don't like to be shook. I will definitely be using a wedge to inoculate.


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Offlinemushbecrazy
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Re: liquid cultures [Re: Pastywhyte]
    #18939426 - 10/06/13 09:45 AM (10 years, 3 months ago)

Guess ill pour out jars, i was reading a thread about cutting a wedge out of BRF vermiculite jar. Had a guidline but no mesurement thats why i asked.


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Re: liquid cultures [Re: mushbecrazy]
    #18939443 - 10/06/13 09:49 AM (10 years, 3 months ago)

P galindoi. One of these jars was shook, the others were not, can you guess which one? Funny thing is that we shake jars to speed them up, but the one that was shook was also inoculated 2 weeks before the others :shrug:



Quote:

mushbecrazy said:
Guess ill pour out jars, i was reading a thread about cutting a wedge out of BRF vermiculite jar. Had a guidline but no mesurement thats why i asked.




Personally that just sounds like a vector for contams to me. I have heard of some people getting away with it, but I'm not gonna try it myself :shrug:


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OfflineRauhfasertapete
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Re: liquid cultures [Re: Pastywhyte]
    #18939464 - 10/06/13 09:53 AM (10 years, 3 months ago)

maybe that one was not completely sterile. Shaking also spreads contaminants.


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Re: liquid cultures [Re: Rauhfasertapete]
    #18939479 - 10/06/13 09:57 AM (10 years, 3 months ago)

Quote:

Rauhfasertapete said:
maybe that one was not completely sterile. Shaking also spreads contaminants.




I'm pretty sure that I can recognize when something is contamed :lol: Its actually still colonizing and forming stones, just really slow. Many TC's especially stonesun and FrankHorrigan have reported stalling from shaking p mex, p tamp, p galindoi, etc.


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OfflineRauhfasertapete
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Re: liquid cultures [Re: Pastywhyte]
    #18939509 - 10/06/13 10:06 AM (10 years, 3 months ago)

you can recognise contamination as soon as it has grown so much that it is visible. but some contams can be in your jar, but not multiply to such amounts that you can see them. Some grows are fucked but you don´t know why or what it is; mycelium may grow, but it may smell strange and won´t fruit. everything´s possible.


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Offlinemushbecrazy
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Re: liquid cultures [Re: Rauhfasertapete]
    #18939542 - 10/06/13 10:13 AM (10 years, 3 months ago)

I use the force when it come to detecting contaminate.


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Re: liquid cultures [Re: mushbecrazy]
    #18939590 - 10/06/13 10:26 AM (10 years, 3 months ago)

Quote:

Rauhfasertapete said:
you can recognise contamination as soon as it has grown so much that it is visible. but some contams can be in your jar, but not multiply to such amounts that you can see them. Some grows are fucked but you don´t know why or what it is; mycelium may grow, but it may smell strange and won´t fruit. everything´s possible.




:bathtub40lol:

Believe me, you ain't telling me anything I don't know but, even if the bacteria's invisible and does not smell (usually becomes visible and does smell after shaking) and the mold mycelium is pure white (won't be for long after you shake it) and looks exactly like p mexicana mycelium (unlikely), you can usually spot a contam from how the mycelium your trying to culture looks at the edge of the uncolonized portion. A hard line, or melted look is usually a great indicator of bacteria, while most molds are easily ID'd just from the look of the mycelium (even trich) but will sporulate given enough time. That jar is 2+ months old. I guarantee that if it stalled due to contam be it bacteria or mold, I would be able to tell by now :shrug:

Quote:

mushbecrazy said:
I use the force when it come to detecting contaminate.



:raisemyglass:


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Offlinekrypto2000
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Re: liquid cultures [Re: mushbecrazy]
    #18939607 - 10/06/13 10:29 AM (10 years, 3 months ago)

Squirt some some sterile h2o into your colonized jar, extract some mycilium, and then squirt that into your LC jar. If your LC and Spawn are both clean it should be fine. If this is with a pf-cake I'm not sure how well it would work though it's hard to breakup inside of the jar and the top verm layer may have contaminants on it which could get into the syringe.


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Offlinemushbecrazy
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Re: liquid cultures [Re: mushbecrazy]
    #18939616 - 10/06/13 10:31 AM (10 years, 3 months ago)

:jiggletits: just have to keep from shaking them good to know


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Re: liquid cultures [Re: mushbecrazy]
    #18939628 - 10/06/13 10:36 AM (10 years, 3 months ago)

The only way to guarantee a clean inoculate for LC is to use an agar wedge. Of course this by no means will guarantee that your LC is clean as it could become contamed through poor procedure ie: contam introduced through inoculation, poor filter, filter gets wet etc. . .


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Offlinemushbecrazy
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Re: liquid cultures [Re: krypto2000]
    #18939630 - 10/06/13 10:36 AM (10 years, 3 months ago)

Quote:

krypto2000 said:
Squirt some some sterile h2o into your colonized jar, extract some mycilium, and then squirt that into your LC jar. If your LC and Spawn are both clean it should be fine. If this is with a pf-cake I'm not sure how well it would work though it's hard to breakup inside of the jar and the top verm layer may have contaminants on it which could get into the syringe.






I got rye jars so this may work :n00bslayer: i was just going to cut a chunk and dunk it in there under flow hood


--------------------
I'm just some lunatic macaroni mushroom, is that it?
-Joe Pesci


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