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Offlineforrest
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Re: Can you use normal pyrex plate as petri dish [Re: MacMerdin]
    #18976699 - 10/14/13 01:47 PM (10 years, 3 months ago)

i first also got a lot condensation with the no-pour agar. but now i transfer the hot jars from the pc to a plastic bin, where they heat the air when they cool down zo they don't condensate as much.


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Invisible36fuckin5
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Re: Can you use normal pyrex plate as petri dish [Re: MacMerdin]
    #18977045 - 10/14/13 03:11 PM (10 years, 3 months ago)

Quote:

MacMerdin said:
How do you get condensation with a filter?




I just do. IDK.


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Invisiblemushmagic
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Re: Can you use normal pyrex plate as petri dish [Re: MacMerdin]
    #18979055 - 10/14/13 10:12 PM (10 years, 3 months ago)

Quote:

MacMerdin said:
How do you get condensation with a filter?




Condensation has nothing to do with there being a filter. It's from the difference of the temperature of the inside and outside of the jar.


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OfflineJahLambsbread
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Re: Can you use normal pyrex plate as petri dish [Re: JahLambsbread]
    #18979253 - 10/14/13 10:56 PM (10 years, 3 months ago)

ok I ordered some petri dishes with agar. do I pc them before the agar is poured in or after?

And can someone tell me a quick way to rehydrate mushroom stem tissue?  just put it in a jar with a wet tissue right?


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OfflinePussyFart
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Re: Can you use normal pyrex plate as petri dish [Re: JahLambsbread]
    #18979278 - 10/14/13 11:05 PM (10 years, 3 months ago)

Quote:

JahLambsbread said:
ok I ordered some petri dishes with agar. do I pc them before the agar is poured in or after?



What kind of dishes, plastic or glass?

The plastic ones should come sterile, so all you would have to do is pour in the sterile agar in a SAB or in front of a flow hood and let set.


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OfflineMacMerdin
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Re: Can you use normal pyrex plate as petri dish [Re: mushmagic]
    #18979983 - 10/15/13 04:11 AM (10 years, 3 months ago)

Quote:

mushmagic said:
Quote:

MacMerdin said:
How do you get condensation with a filter?




Condensation has nothing to do with there being a filter. It's from the difference of the temperature of the inside and outside of the jar.




But once the difference in temperature levels out the condensation should also go away (or rather evaporate through the filter).  So, yes, a filter DOES have something to do with it.  Or does a glass of ice water ALWAYS have condensation on the outside even when the glass of water has become room temperature?  No?  Why?  Because the condensation evaporated once the glass became the same temperature as the air.  Same thing happens inside a jar if you have a filter that allows moisture exchange.

Also, maybe he used SFDs which are moisture proof (are they moisture proof when the moisture is in the gaseous stage?).  Or maybe letting my jars cool in the PC (never being a differential in temperature from glass to air inside PC) has something to do with it?

All I know is I can show photos of the 6 jars I have right now with ZERO condensation in them.

I never even thought condensation was an issue with these until today.  :confused:

p.s.  I use capitals to emphasize not yell, so please don't take offense to my postings.


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Invisiblemushmagic
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Re: Can you use normal pyrex plate as petri dish [Re: MacMerdin]
    #18980443 - 10/15/13 08:40 AM (10 years, 3 months ago)

Quote:

MacMerdin said:
Quote:

mushmagic said:
Quote:

MacMerdin said:
How do you get condensation with a filter?




Condensation has nothing to do with there being a filter. It's from the difference of the temperature of the inside and outside of the jar.




But once the difference in temperature levels out the condensation should also go away (or rather evaporate through the filter).  So, yes, a filter DOES have something to do with it.  Or does a glass of ice water ALWAYS have condensation on the outside even when the glass of water has become room temperature?  No?  Why?  Because the condensation evaporated once the glass became the same temperature as the air.  Same thing happens inside a jar if you have a filter that allows moisture exchange.

Also, maybe he used SFDs which are moisture proof (are they moisture proof when the moisture is in the gaseous stage?).  Or maybe letting my jars cool in the PC (never being a differential in temperature from glass to air inside PC) has something to do with it?

All I know is I can show photos of the 6 jars I have right now with ZERO condensation in them.

I never even thought condensation was an issue with these until today.  :confused:

p.s.  I use capitals to emphasize not yell, so please don't take offense to my postings.




No offense taken. I'm not totally sure about the condensation leveling out because yea, sometimes the condensation still seems to happen even when you leave it in the PC to completely cool down 100% to room temp before taking it out for some reason. However the filter really won't let the moisture evaporate that fast. I've had some leftover grains in the bottom of a jar from G2G a while back and decided to try and dry them out in the filtered jar for shits and giggles because I had heard of storing dry colonized grains for later use but they just did not seem to dry out at all even after weeks of sitting there. Maybe I'll just have to try spraying the wall of a jar with a filtered lid and see how long it takes to evaporate.


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Re: Can you use normal pyrex plate as petri dish [Re: mushmagic]
    #18980515 - 10/15/13 09:05 AM (10 years, 3 months ago)

The natural temperature of glass and metal is slightly lower than ambient air temperature.

Your agar jars also have a high absolute humidity.

These two things together make it difficult for condensation to dissipate inside the jar.

A high absolute humidity results in condensation even with only a slight temperature differential.

Condensation on your glass of ice water can form in low absolute humidity because the temperature differential is vast between the glass and the ambient air.

Although the terms absolute and relative humidity can and often are used interchangeably, they have distinct definitions.


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OfflineTheBoomking
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Re: Can you use normal pyrex plate as petri dish [Re: MacMerdin]
    #18980543 - 10/15/13 09:14 AM (10 years, 3 months ago)

These are what I use:



Advantages:  Can pre-pour the agar (also known as no-pour) and then PC.  No need to pour the agar after PCing (ideal if you don't have a flow hood IMO).

Disadvantages:  Can not see through the lid so a sideways or bottom view is only possible.




yeah I used this as well as a substitute for petri dishes. the disadvantage with not being able to see easily is a huge disadvantage in my experience. especially when testing old syringes to see if they're still viable. I have growth but can;t see well enough to make be sure its the right mycelium and not mold. A great all around substitute nonetheless. :thumbup:


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Offlinehamloaf
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Re: Can you use normal pyrex plate as petri dish [Re: TheBoomking]
    #18980563 - 10/15/13 09:22 AM (10 years, 3 months ago)

Try looking at your cultures in those jars from the bottom.  It's not the best view but the whole culture can be seen from that angle.  Get parafilm.  It's way easier to work with over saran wrap and costs just a dollar more or something.


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